Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... containing plasmid DNAand 10pg sonicated chicken erythrocyte (carrier) DNA, prepared as described above. Plating in regeneration agar on SD selective media was followed by incubation at 30" for 4-5 days. Selection for Leu+, Trp+, orUra+ transformants was made in the presence of a growth-limiting qua ...
... containing plasmid DNAand 10pg sonicated chicken erythrocyte (carrier) DNA, prepared as described above. Plating in regeneration agar on SD selective media was followed by incubation at 30" for 4-5 days. Selection for Leu+, Trp+, orUra+ transformants was made in the presence of a growth-limiting qua ...
345 - Timstar
... THE POLYMERASE CHAIN REACTION (PCR) The PCR reaction is a DNA amplification technique that revolutionized almost all aspects of biological research. The procedure was invented by Dr. Kary Mullis while at Cetus Corporation in 1984. Dr. Mullis was awarded a Nobel Prize for his work in 1994. PCR amplif ...
... THE POLYMERASE CHAIN REACTION (PCR) The PCR reaction is a DNA amplification technique that revolutionized almost all aspects of biological research. The procedure was invented by Dr. Kary Mullis while at Cetus Corporation in 1984. Dr. Mullis was awarded a Nobel Prize for his work in 1994. PCR amplif ...
Extension Activity 1: Plasmid Mapping STUDENT MANU AL
... viruses that infect and destroy bacteria. Restriction enzymes recognize specific DNA sequences within the phage DNA and then cut the DNA at that site. Fragmented DNA no longer poses a threat to bacterial survival. Purified restriction enzymes can be used in the laboratory to cut DNA isolated from an ...
... viruses that infect and destroy bacteria. Restriction enzymes recognize specific DNA sequences within the phage DNA and then cut the DNA at that site. Fragmented DNA no longer poses a threat to bacterial survival. Purified restriction enzymes can be used in the laboratory to cut DNA isolated from an ...
Application Note: Targeted sequencing and chromosomal haplotype
... using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer p ...
... using just one primer pair complementary to a short locusspecific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer p ...
Molecular Diagnostics in Clinical Microbiology
... forward. Nucleic acid from the potential pathogen is extracted from the clinical sample, subsequently followed by an amplification-detection protocol, preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective s ...
... forward. Nucleic acid from the potential pathogen is extracted from the clinical sample, subsequently followed by an amplification-detection protocol, preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective s ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... limits of optical tweezers and what are the parameters that define these limits? In most cases, the performance of an optical tweezers instrument is limited by instrument drift and environmental noise. These factors can, to a large extent be suppressed by placing instruments in sound-isolated, tempe ...
... limits of optical tweezers and what are the parameters that define these limits? In most cases, the performance of an optical tweezers instrument is limited by instrument drift and environmental noise. These factors can, to a large extent be suppressed by placing instruments in sound-isolated, tempe ...
e Study of RNA Polymerase Pausing by Optical Traps
... RNAP molecules transcribing the his pause DNA template. Although there is a positional uncertainty error of up to 200 bp, the traces suggest regularly spaced pauses of eight concatenated sequences. Periodic pauses are observed and the two traces appear to have some correlation in terms of aligning t ...
... RNAP molecules transcribing the his pause DNA template. Although there is a positional uncertainty error of up to 200 bp, the traces suggest regularly spaced pauses of eight concatenated sequences. Periodic pauses are observed and the two traces appear to have some correlation in terms of aligning t ...
Supplementary Materials and methods (doc 154K)
... DNA extraction, quantification and digestion. To analyse the plasmid copy numbers in the different constructions, three to six independent DNA extractions were done per strain and qPCR was performed for each extraction in triplicate. DNA extractions ...
... DNA extraction, quantification and digestion. To analyse the plasmid copy numbers in the different constructions, three to six independent DNA extractions were done per strain and qPCR was performed for each extraction in triplicate. DNA extractions ...
The first page should show the paper title, names and addresses of
... chromosome paints were labeled with biotin-dUTP or digoxigenin-dUTP in a DOP-PCR using 6MW primer (Telenius et al. 1992). Oligonucleotide probes specific for chicken CNM (Matzke et al. 1990) and quail BglII-repeat (Tanaka et al. 2000) were also used for centromere detection, namely, CNMpos, CNMneg, ...
... chromosome paints were labeled with biotin-dUTP or digoxigenin-dUTP in a DOP-PCR using 6MW primer (Telenius et al. 1992). Oligonucleotide probes specific for chicken CNM (Matzke et al. 1990) and quail BglII-repeat (Tanaka et al. 2000) were also used for centromere detection, namely, CNMpos, CNMneg, ...
Molecular events during translocation and proofreading extracted
... definition of conformational space with limited dimensionality (12). Each experimentally determined static structure is a snapshot of the protein structure. See (15,16) for examples within the context of polymerase structures. A large number of such snapshots taken under a variety of experimental co ...
... definition of conformational space with limited dimensionality (12). Each experimentally determined static structure is a snapshot of the protein structure. See (15,16) for examples within the context of polymerase structures. A large number of such snapshots taken under a variety of experimental co ...
DNA breathing dynamics distinguish binding from nonbinding
... fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG ...
... fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG ...
Full-Text PDF
... performed in centralised labs with expert users and specialized equipment, although there are several examples of portable systems that can be used in the field. Multiplexed PCR assays for simultaneous detection of resistance genes have also been developed [19–21]; however, although PCR is the gold ...
... performed in centralised labs with expert users and specialized equipment, although there are several examples of portable systems that can be used in the field. Multiplexed PCR assays for simultaneous detection of resistance genes have also been developed [19–21]; however, although PCR is the gold ...
Supplementary Figure Legends (doc 34K)
... exons 49 to 57. NF1 exons named according to NCBI nomenclature (exons numbered 1-58) are represented by rectangles (not proportional to their size) at the genomic level and at the corresponding cDNA level. Exons 49 to 57 duplication was studied at the cDNA level. After reverse transcription of NF1 m ...
... exons 49 to 57. NF1 exons named according to NCBI nomenclature (exons numbered 1-58) are represented by rectangles (not proportional to their size) at the genomic level and at the corresponding cDNA level. Exons 49 to 57 duplication was studied at the cDNA level. After reverse transcription of NF1 m ...
Chpt8_RecombineDNA.doc
... from two parental DNA molecules or different segments of the same DNA molecule; this will be the topic of this chapter. Transposition is a highly specialized form of recombination in which a segment of DNA moves from one location to another, either on the same chromosome or a different chromosome; t ...
... from two parental DNA molecules or different segments of the same DNA molecule; this will be the topic of this chapter. Transposition is a highly specialized form of recombination in which a segment of DNA moves from one location to another, either on the same chromosome or a different chromosome; t ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... storing DNA, RNA and amino-acid sequences is increasing exponentially (Matsumoto et al., 2000). As an example, the lengths of the 24 chromosomes in human are found to have 50 to 250 million base pairs (Human Genome Project Science, http://www.ornl.gov/sci/techresources/Human_Genome/project/info.shtm ...
... storing DNA, RNA and amino-acid sequences is increasing exponentially (Matsumoto et al., 2000). As an example, the lengths of the 24 chromosomes in human are found to have 50 to 250 million base pairs (Human Genome Project Science, http://www.ornl.gov/sci/techresources/Human_Genome/project/info.shtm ...
pdf
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
View PDF - OMICS International
... errors, 45% zygotes appeared to be balanced following these sequential errors, representing a phenomenon of aneuploidy rescue [Table 3]. The fate of the embryos resulting from such balanced oocytes is not understood, but may lead to the formation of mosaic embryos, or to those with uniparental disom ...
... errors, 45% zygotes appeared to be balanced following these sequential errors, representing a phenomenon of aneuploidy rescue [Table 3]. The fate of the embryos resulting from such balanced oocytes is not understood, but may lead to the formation of mosaic embryos, or to those with uniparental disom ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.