Evidence Suggesting that Pif1 Helicase Functions in DNA
... addition of telomeres at HO-endonuclease-induced doublestrand breaks (DSBs) and a high level of gross chromosomal rearrangements (GCRs) in which there is excessive telomere addition to nontelomeric sequences at sites of intrachromosomal DNA damage, presumably primarily DSBs (36, 42, 50). By analogy ...
... addition of telomeres at HO-endonuclease-induced doublestrand breaks (DSBs) and a high level of gross chromosomal rearrangements (GCRs) in which there is excessive telomere addition to nontelomeric sequences at sites of intrachromosomal DNA damage, presumably primarily DSBs (36, 42, 50). By analogy ...
Peptide nucleic acids 2
... 4.3 N M R studies of P N A - D N A and P N A - R N A hybrids . . . . . . . . . . . . . . . . . . . . . . . . . 4.4 Crystal structures of P N A - D N A complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5. PNA as a Potential Antisense and Antigene Drug . . . . . . . . . . . . . . . . ...
... 4.3 N M R studies of P N A - D N A and P N A - R N A hybrids . . . . . . . . . . . . . . . . . . . . . . . . . 4.4 Crystal structures of P N A - D N A complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5. PNA as a Potential Antisense and Antigene Drug . . . . . . . . . . . . . . . . ...
PCR and qPCR product guide
... (Taq)4. This polymerase could withstand the high temperatures required during the denaturation and extension steps of PCR, and thus fresh enzyme was not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s ...
... (Taq)4. This polymerase could withstand the high temperatures required during the denaturation and extension steps of PCR, and thus fresh enzyme was not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s ...
PO 4
... Ribose is a sugar, like glucose, but with only five carbon atoms in its molecule Deoxyribose is almost the same but lacks one oxygen atom ...
... Ribose is a sugar, like glucose, but with only five carbon atoms in its molecule Deoxyribose is almost the same but lacks one oxygen atom ...
PO 4
... Ribose is a sugar, like glucose, but with only five carbon atoms in its molecule Examples:mRNA, tRNA, and rRNA Deoxyribose is almost the same but lacks one oxygen atom Example:DNA Both molecules may be represented by the symbol ...
... Ribose is a sugar, like glucose, but with only five carbon atoms in its molecule Examples:mRNA, tRNA, and rRNA Deoxyribose is almost the same but lacks one oxygen atom Example:DNA Both molecules may be represented by the symbol ...
DNA . ppt - biology
... Ribose is a sugar, like glucose, but with only five carbon atoms in its molecule Deoxyribose is almost the same but lacks one oxygen atom ...
... Ribose is a sugar, like glucose, but with only five carbon atoms in its molecule Deoxyribose is almost the same but lacks one oxygen atom ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be detected independently in the same vial (a different form of “multiplexing”). Since ...
... agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be detected independently in the same vial (a different form of “multiplexing”). Since ...
Control of DNA excision efficiency in Paramecium
... within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk. INTRODUCTION Programmed DNA rearrangements occur in a wide range of ...
... within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk. INTRODUCTION Programmed DNA rearrangements occur in a wide range of ...
Dissection of molecular interactions of replication protein A in
... Replication protein A (RPA) is the major eukaryotic single-strand DNA (ssDNA) binding protein. RPA is composed of three subunits, RPA1, RPA2 and RPA3. RPA is essential for replication, repair, recombination, and checkpoint activation, and is required for maintaining genome integrity. In the cell, RP ...
... Replication protein A (RPA) is the major eukaryotic single-strand DNA (ssDNA) binding protein. RPA is composed of three subunits, RPA1, RPA2 and RPA3. RPA is essential for replication, repair, recombination, and checkpoint activation, and is required for maintaining genome integrity. In the cell, RP ...
Effect of bile salts on the DNA and membrane integrity of enteric
... pathogenic potential of an enteric bacterium is directly related to its ability to grow in the presence of bile salts. However, to determine if this hypothesis is true the mechanisms by which bacteria are able to grow in bile salt environments need to be ...
... pathogenic potential of an enteric bacterium is directly related to its ability to grow in the presence of bile salts. However, to determine if this hypothesis is true the mechanisms by which bacteria are able to grow in bile salt environments need to be ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... that time, one can not but be impressed with Peacocke’s courage and vision at undertaking this research direction. Results of these studies were published in journals that are to this day considered to be among the most prestigious. From that time until the end of his research carrier, Peacocke’s ma ...
... that time, one can not but be impressed with Peacocke’s courage and vision at undertaking this research direction. Results of these studies were published in journals that are to this day considered to be among the most prestigious. From that time until the end of his research carrier, Peacocke’s ma ...
Mcm10: A Dynamic Scaffold at Eukaryotic Replication Forks
... Abstract: To complete the duplication of large genomes efficiently, mechanisms have evolved that coordinate DNA unwinding with DNA synthesis and provide quality control measures prior to cell division. Minichromosome maintenance protein 10 (Mcm10) is a conserved component of the eukaryotic replisome ...
... Abstract: To complete the duplication of large genomes efficiently, mechanisms have evolved that coordinate DNA unwinding with DNA synthesis and provide quality control measures prior to cell division. Minichromosome maintenance protein 10 (Mcm10) is a conserved component of the eukaryotic replisome ...
Two Components of the RNA-Directed DNA Methylation Pathway
... domain and mediates the recruitment of Pol IV to chromatin at a subset of RdDM target loci [30,31]. DMS3, DRD1, and RDM1 are three important components in the RdDM pathway [32–34] and form a DDR complex required for Pol V occupancy on chromatin [17,35,36]. SUVH2 and SUVH9, which belong to the SRA do ...
... domain and mediates the recruitment of Pol IV to chromatin at a subset of RdDM target loci [30,31]. DMS3, DRD1, and RDM1 are three important components in the RdDM pathway [32–34] and form a DDR complex required for Pol V occupancy on chromatin [17,35,36]. SUVH2 and SUVH9, which belong to the SRA do ...
KingFisher Pure DNA Plant Kit
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
Damage response of XRCC1 at sites of DNA single strand breaks is
... To follow the damage response of XRCC1 in real time, we used laser micro-irradiation through an objective lens that predominantly produces SSBs in restricted sites within a single cell nucleus in real time (Lan et al., 2004). We followed the dynamics of XRCC1 in a single cell in real time using the ...
... To follow the damage response of XRCC1 in real time, we used laser micro-irradiation through an objective lens that predominantly produces SSBs in restricted sites within a single cell nucleus in real time (Lan et al., 2004). We followed the dynamics of XRCC1 in a single cell in real time using the ...
EndoFree® Plasmid Purification Handbook
... Do not exceed the maximum recommended culture volumes given at the beginning of each protocol (and on the card inside the back cover of this handbook). Using larger culture volumes will lead to an increase in biomass and can affect the efficiency of alkaline lysis, leading to reduced yield and purit ...
... Do not exceed the maximum recommended culture volumes given at the beginning of each protocol (and on the card inside the back cover of this handbook). Using larger culture volumes will lead to an increase in biomass and can affect the efficiency of alkaline lysis, leading to reduced yield and purit ...
Properties of Mitotic and Meiotic Recombination in the
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
Introduction - HAL
... The participation of pol µ in the DNA end processing of light chain and that of pol in heavy chain gene rearrangement raises the question whether the two DNA polymerase activities might partially compensate for each other during V(D)J recombination. Pol µ/pol double knockout mice are viable and ...
... The participation of pol µ in the DNA end processing of light chain and that of pol in heavy chain gene rearrangement raises the question whether the two DNA polymerase activities might partially compensate for each other during V(D)J recombination. Pol µ/pol double knockout mice are viable and ...
Replication of damaged DNA in vitro is blocked
... we examined the ability of various forms of p53, each differing in their ability to bind to DNA, to inhibit the replication of Vw16PyCAT in mouse FM3A cell extracts. Note that although FM3A cells have been reported to contain wild-type p53 whose protein levels are increased after UV irradiation (40) ...
... we examined the ability of various forms of p53, each differing in their ability to bind to DNA, to inhibit the replication of Vw16PyCAT in mouse FM3A cell extracts. Note that although FM3A cells have been reported to contain wild-type p53 whose protein levels are increased after UV irradiation (40) ...
Single-stranded heteroduplex intermediates in l Red homologous
... single stranded heteroduplexes at the replication fork. We created asymmetrically digestible dsDNA substrates by exploiting the fact that Reda exonuclease activity requires a 5' phosphorylated end, or is blocked by phosphothioates. Using these substrates, we found that the most efficient configurati ...
... single stranded heteroduplexes at the replication fork. We created asymmetrically digestible dsDNA substrates by exploiting the fact that Reda exonuclease activity requires a 5' phosphorylated end, or is blocked by phosphothioates. Using these substrates, we found that the most efficient configurati ...
Identification of a Preinitiation Step in DNA Replication That Is
... Using footprinting patterns to examine protein–DNA interactions at origin sequences in yeast, Diffley and coworkers have shown that origins oscillate between two states during the cell cycle. The prereplicative state forms during G1 and requires both ORC and cdc6 proteins (Cocker et al., 1996; Santo ...
... Using footprinting patterns to examine protein–DNA interactions at origin sequences in yeast, Diffley and coworkers have shown that origins oscillate between two states during the cell cycle. The prereplicative state forms during G1 and requires both ORC and cdc6 proteins (Cocker et al., 1996; Santo ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.