Phylogenomics of type II DNA topoisomerases
... reminiscent of many other enzymes involved in DNA metabolism (e.g. DNA polymerases, primases, DNA helicases, thymidylate synthases and so on).(19) Evolution of the two bacterial Topo IIA All Topo II from Bacteria belong to the A family. This is quite significant, considering the number and variety o ...
... reminiscent of many other enzymes involved in DNA metabolism (e.g. DNA polymerases, primases, DNA helicases, thymidylate synthases and so on).(19) Evolution of the two bacterial Topo IIA All Topo II from Bacteria belong to the A family. This is quite significant, considering the number and variety o ...
Recognition of Local DNA Structures by p53 Protein
... crowding and chromatin accessibility [35]. Triplex-forming sequences are abundant, especially in eukaryotes [36,37]. Most polypurine-polypyrimidine DNA tracks are found in introns, promoters, and 50 and 30 untranslated regions. It was shown that triplex-forming sequences are enriched in genes relate ...
... crowding and chromatin accessibility [35]. Triplex-forming sequences are abundant, especially in eukaryotes [36,37]. Most polypurine-polypyrimidine DNA tracks are found in introns, promoters, and 50 and 30 untranslated regions. It was shown that triplex-forming sequences are enriched in genes relate ...
SURVEY AND SUMMARY New insights into Hoogsteen base pairs
... which the adenine base was flipped 180◦ to form a unique set of hydrogen bonds (H-bonds) (Figure 1). Two years earlier, Rich et al. had speculated that such a pairing scheme could explain how poly(rU) associates with poly(rA)-poly(rU) duplexes to form RNA triplexes (2). These bps are now referred to ...
... which the adenine base was flipped 180◦ to form a unique set of hydrogen bonds (H-bonds) (Figure 1). Two years earlier, Rich et al. had speculated that such a pairing scheme could explain how poly(rU) associates with poly(rA)-poly(rU) duplexes to form RNA triplexes (2). These bps are now referred to ...
CC 1 070-71..8171c ose chapter .. Page70
... classes of molecules with high triplex affinity can be identified. A pyrene–neomycin conjugate has therefore been synthesized by forming an amide linkage between neomycin B and 1-pyrenebutyric acid N-hydroxysuccinimide ester (Fig. 1). We report that pyrene–neomycin is more potent in stabilizing DNA ...
... classes of molecules with high triplex affinity can be identified. A pyrene–neomycin conjugate has therefore been synthesized by forming an amide linkage between neomycin B and 1-pyrenebutyric acid N-hydroxysuccinimide ester (Fig. 1). We report that pyrene–neomycin is more potent in stabilizing DNA ...
Non-homologous end-joining partners in a helical dance: structural
... proteins in the human NHEJ (non-homologous end-joining) pathway that repairs DNA DSBs (doublestrand breaks). The individual crystal structures show that the dimeric proteins are homologues with protomers containing head domains and helical coiled-coil tails related by approximate two-fold symmetry. ...
... proteins in the human NHEJ (non-homologous end-joining) pathway that repairs DNA DSBs (doublestrand breaks). The individual crystal structures show that the dimeric proteins are homologues with protomers containing head domains and helical coiled-coil tails related by approximate two-fold symmetry. ...
Centromere Stability: The Replication Connection
... cell’s damage response [42,50]. If RPA levels are reduced, DNA breakage occurs [51]. Thus, the amount of ssDNA produced during stress helps to modulate the appropriate response. The classic cell cycle model suggests that that accumulation of ssDNA and replication stress activate a checkpoint signali ...
... cell’s damage response [42,50]. If RPA levels are reduced, DNA breakage occurs [51]. Thus, the amount of ssDNA produced during stress helps to modulate the appropriate response. The classic cell cycle model suggests that that accumulation of ssDNA and replication stress activate a checkpoint signali ...
An Introduction to Peptide Nucleic Acid
... repulsion between an incoming PNA probe and a mass of DNA or RNA in a Southern blot, northern blot or in situ hybridization experiment. This means that the rate of hybridization is much higher for PNA probes than for DNA and RNA probes in these applications. Typically a more than 10-fold reduction i ...
... repulsion between an incoming PNA probe and a mass of DNA or RNA in a Southern blot, northern blot or in situ hybridization experiment. This means that the rate of hybridization is much higher for PNA probes than for DNA and RNA probes in these applications. Typically a more than 10-fold reduction i ...
Human DNA Ligase IV and the Ligase IV/XRCC4 Complex: Analysis
... complex has not been studied in this regard. Ligase IV/XRCC4 is the sole DNA ligase involved in the repair of double strand breaks (DSBs) via the non-homologous end joining (NHEJ) pathway. During the repair of DSBs generated by chemical/physical damage as well as the repair of the programmed DSB int ...
... complex has not been studied in this regard. Ligase IV/XRCC4 is the sole DNA ligase involved in the repair of double strand breaks (DSBs) via the non-homologous end joining (NHEJ) pathway. During the repair of DSBs generated by chemical/physical damage as well as the repair of the programmed DSB int ...
Bacillus subtilis serine/threonine protein kinase YabT is involved in
... attention to in vivo assays. YabT-encoding gene was previously reported to be expressed in a SigF-dependent manner (Wang et al., 2006). SigF is a special sigma factor dedicated to the regulation of spore development in B. subtilis (Haldenwang, 1995). When exposed to lack of nutrients, the ultimate s ...
... attention to in vivo assays. YabT-encoding gene was previously reported to be expressed in a SigF-dependent manner (Wang et al., 2006). SigF is a special sigma factor dedicated to the regulation of spore development in B. subtilis (Haldenwang, 1995). When exposed to lack of nutrients, the ultimate s ...
Meiotic Recombination inSchizosaccharomyces pombe: A Paradigm
... or the homolog (stage 3). Only interhomolog interaction gives rise to the physical connections (chiasmata) that aid homolog segregation at MI, but sister chromatid exchange does occur. The regulation of these two types of repair is an intriguing problem not yet solved. DSB repair occurs in steps. Fi ...
... or the homolog (stage 3). Only interhomolog interaction gives rise to the physical connections (chiasmata) that aid homolog segregation at MI, but sister chromatid exchange does occur. The regulation of these two types of repair is an intriguing problem not yet solved. DSB repair occurs in steps. Fi ...
Telomeres do the (un)twist: helicase actions at chromosome termini
... telomere length is heritable and associated with parental lifespan [25, 26]. Similarly, increased telomere length is correlated with improved left ventricular function and reduced cardiovascular disease risk, improved bone density and oocyte function, and reduced poststroke mortality and dementia [2 ...
... telomere length is heritable and associated with parental lifespan [25, 26]. Similarly, increased telomere length is correlated with improved left ventricular function and reduced cardiovascular disease risk, improved bone density and oocyte function, and reduced poststroke mortality and dementia [2 ...
Have a break: determinants of meiotic DNA double strand break
... sequences are tightly packed, leaving hardly any space for heterochromatin (Zickler and Kleckner, 1999). The genomes of higher eukaryotes, in contrast, are often extremely large, and the coordination of DSB formation throughout the genome may rely even more on epigenetic marks. Work performed by the ...
... sequences are tightly packed, leaving hardly any space for heterochromatin (Zickler and Kleckner, 1999). The genomes of higher eukaryotes, in contrast, are often extremely large, and the coordination of DSB formation throughout the genome may rely even more on epigenetic marks. Work performed by the ...
microencapsula tion of dna within cross
... are important risk factors for certain types of cancel Within the intestinal tract there exists a wide range of potentially toxic substances which may be a causative factor in cancer of the lower intestinal tract ...
... are important risk factors for certain types of cancel Within the intestinal tract there exists a wide range of potentially toxic substances which may be a causative factor in cancer of the lower intestinal tract ...
Press release
... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
Analysis of clones carrying repeated DNA sequences in two YAC
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
2. Assessing the probative value of DNA evidence
... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
Defining characteristics of Tn5 Transposase non
... amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific DNA (4). The Tn3 Tnp binds non-specific linear restriction fragments (5,6). Finally, many Tnps, including Tn5 Tnp, can integrate their transposons into random DNA sequences (7 ...
... amino acids of domain III binds supercoiled DNA nonspecifically (3) and that domain II can interact with linear non-specific DNA (4). The Tn3 Tnp binds non-specific linear restriction fragments (5,6). Finally, many Tnps, including Tn5 Tnp, can integrate their transposons into random DNA sequences (7 ...
Assessing the Probative Value of DNA Evidence
... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
Investigating the DNA-Binding Interactions of Small
... Cancer is one the leading causes of death in the United States. Researchers have focused on designing and developing therapeutics aimed at DNA in order to treat cancer and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a ...
... Cancer is one the leading causes of death in the United States. Researchers have focused on designing and developing therapeutics aimed at DNA in order to treat cancer and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a ...
Graph-based Methods for the Design of DNA - TUBdok
... evaluations of several DNA word sets from the literature is performed. 2. A new approach is developed for solving a minimal free energy (MFE) problem for the hybridization complex built by a pair of partially complementary DNA molecules. It introduces a concept of the hybridization graph to represen ...
... evaluations of several DNA word sets from the literature is performed. 2. A new approach is developed for solving a minimal free energy (MFE) problem for the hybridization complex built by a pair of partially complementary DNA molecules. It introduces a concept of the hybridization graph to represen ...
quantitation of male and female dna in mixed biological samples
... and a Y chromosome from their father and therefore males carry the XY genotype. Normal females receive an X chromosome from their mother and the other X chromosome from their father and are genotypically XX (Butler, 2005) (Figure 1). ...
... and a Y chromosome from their father and therefore males carry the XY genotype. Normal females receive an X chromosome from their mother and the other X chromosome from their father and are genotypically XX (Butler, 2005) (Figure 1). ...
Mutagenesis Point mutations Deletions Insertions Types of
... vectors, which are then transformed into E. coli. One or more fragments have been routinely assembled with general cloning vectors, such as pUC19, and assembled into NEB’s pTYB1 expression vector (NEB #N6701). The latter approach was used to express several methylase genes, which aided the genome tr ...
... vectors, which are then transformed into E. coli. One or more fragments have been routinely assembled with general cloning vectors, such as pUC19, and assembled into NEB’s pTYB1 expression vector (NEB #N6701). The latter approach was used to express several methylase genes, which aided the genome tr ...
Ciz1 cooperates with cyclin-A–CDK2 to activate mammalian DNA
... first terminating the assembly stage by phosphorylating and inactivating CDC6 and then, as its levels rise in late G1, activating the DNA replication machinery. Reversal of the natural order in which nuclei are exposed to cyclins E and A blocks initiation in vitro (Coverley et al., 2002), highlighti ...
... first terminating the assembly stage by phosphorylating and inactivating CDC6 and then, as its levels rise in late G1, activating the DNA replication machinery. Reversal of the natural order in which nuclei are exposed to cyclins E and A blocks initiation in vitro (Coverley et al., 2002), highlighti ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.