2 - cellbiochem.ca
... bacterial DNA polymerase to bind and initiate DNA replication; • Several Ori can be found. ColE1 is the most frequently encountered; • Some Ori allow the plasmid to replicate frequently (high copy number plasmids – up to 100 copies per cell); others allow only a low rate of replication initiation (l ...
... bacterial DNA polymerase to bind and initiate DNA replication; • Several Ori can be found. ColE1 is the most frequently encountered; • Some Ori allow the plasmid to replicate frequently (high copy number plasmids – up to 100 copies per cell); others allow only a low rate of replication initiation (l ...
dissertationes biologicae universitatis tartuensis 118
... they both comprise a helix-hairpin-helix motif that is commonly involved in nonspecific double-stranded DNA (dsDNA) binding (Shao and Grishin, 2000). The ATP binding site is situated in a cleft between domains 1A and 2A that is lined with conserved helicase motifs (Fig. 1B). The Rep helicase was cry ...
... they both comprise a helix-hairpin-helix motif that is commonly involved in nonspecific double-stranded DNA (dsDNA) binding (Shao and Grishin, 2000). The ATP binding site is situated in a cleft between domains 1A and 2A that is lined with conserved helicase motifs (Fig. 1B). The Rep helicase was cry ...
Deconstructing the Genome: DNA at High Resolution
... released from the nuclei of cells at specific sites. These welldefined cuts generate fragments suitable for manipulation and characterization. A restriction enzyme recognizes a specific sequence of bases anywhere within the genome and then severs two covalent bonds (one in each strand) in the sugar- ...
... released from the nuclei of cells at specific sites. These welldefined cuts generate fragments suitable for manipulation and characterization. A restriction enzyme recognizes a specific sequence of bases anywhere within the genome and then severs two covalent bonds (one in each strand) in the sugar- ...
Non-Canonical Replication Initiation: You`re Fired!
... (origin recognition complex, Cdc6, and MCM proteins are sufficient to re-initiate DNA replication in G2/M cells. In the latter case, a Mec1 and Mre11-Rad50-Xrs2 (MRX) complex-dependent DNA damage signaling pathway is activated to restrain the extent of re-replication and to promote survival when ori ...
... (origin recognition complex, Cdc6, and MCM proteins are sufficient to re-initiate DNA replication in G2/M cells. In the latter case, a Mec1 and Mre11-Rad50-Xrs2 (MRX) complex-dependent DNA damage signaling pathway is activated to restrain the extent of re-replication and to promote survival when ori ...
Invisorb Spin Plasmid Mini Two
... Kits, since richer broths such as TB (Terrific Broth) or 2x YT lead to extremely high cell densities, which can overload the ...
... Kits, since richer broths such as TB (Terrific Broth) or 2x YT lead to extremely high cell densities, which can overload the ...
The Art of DNA Strings: Sixteen Years of DNA Coding Theory
... match. But in some situation, DNA strands may not form perfect base pairing and react in undesirable manner. One situation is formation of secondary structure in which first half strand of the DNA strand forms complementary with its own other half forming the hair pin like structure. This kind of st ...
... match. But in some situation, DNA strands may not form perfect base pairing and react in undesirable manner. One situation is formation of secondary structure in which first half strand of the DNA strand forms complementary with its own other half forming the hair pin like structure. This kind of st ...
EPICENTRE Enzyme Catalog
... activity. Klenow DNA polymerase blunt ends doublestranded DNA with singlestranded overhangs. The 3´→5´ exonuclease activity removes 3´ overhangs and the 5´→3´ polymerization activity fills in 5´ overhangs. ...
... activity. Klenow DNA polymerase blunt ends doublestranded DNA with singlestranded overhangs. The 3´→5´ exonuclease activity removes 3´ overhangs and the 5´→3´ polymerization activity fills in 5´ overhangs. ...
SNP Analysis of the PTC Gene Using PCR
... from individuals who show differences in their ability to taste PTC. In addition to the two primers, four deoxynucleotides (dATP, dCTP, dGTP, and dTTP) and a thermally stable DNA polymerase are required. The most commonly used DNA polymerase is the enzyme Taq polymerase, which is purified from the th ...
... from individuals who show differences in their ability to taste PTC. In addition to the two primers, four deoxynucleotides (dATP, dCTP, dGTP, and dTTP) and a thermally stable DNA polymerase are required. The most commonly used DNA polymerase is the enzyme Taq polymerase, which is purified from the th ...
Construction of plant BAC libraries This document
... incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place the stained gel pieces aside. 13. Using a scalpel, coverglass, or razor blade, connect the incisions on each even-numbered gel piece as shown in FIGURE 2e. You should now have three unst ...
... incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place the stained gel pieces aside. 13. Using a scalpel, coverglass, or razor blade, connect the incisions on each even-numbered gel piece as shown in FIGURE 2e. You should now have three unst ...
Chapter 11 - Nucleic Acid Structure and DNA Replication
... namely, proteins and DNA. Scientists of this era viewed proteins as being more biochemically complex because they are made from 20 different amino acids. Furthermore, biochemists already knew that proteins perform an amazingly wide range of functions. Complexity seemed an important prerequisite for ...
... namely, proteins and DNA. Scientists of this era viewed proteins as being more biochemically complex because they are made from 20 different amino acids. Furthermore, biochemists already knew that proteins perform an amazingly wide range of functions. Complexity seemed an important prerequisite for ...
Detection of Agrobacterium vitis by polymerase chain reaction in
... are lysed under these conditions. Therefore, the use of the simple boiling method in distilled water or Triton X-100 is appropriate only for pure cultures and isolated colonies. To increase the sensitivity of detection we have tested the effect of NaN3 with Triton X-100 (ABOLMAATY et al. 2000). This ...
... are lysed under these conditions. Therefore, the use of the simple boiling method in distilled water or Triton X-100 is appropriate only for pure cultures and isolated colonies. To increase the sensitivity of detection we have tested the effect of NaN3 with Triton X-100 (ABOLMAATY et al. 2000). This ...
The PCNA–RFC Families of DNA Clamps and Clamp
... The advantages of having a clamp–DNA polymerase complex for performing processive DNA replication, rather than a processive DNA polymerase because of its strong DNA-binding properties, is that the former complex in principle allows for facile release of torsional stress. As the DNA polymerase replic ...
... The advantages of having a clamp–DNA polymerase complex for performing processive DNA replication, rather than a processive DNA polymerase because of its strong DNA-binding properties, is that the former complex in principle allows for facile release of torsional stress. As the DNA polymerase replic ...
reactive_oxygen_species
... to produce DNA damage and tumorigenesis. Nrf2(a basic-region leucine zipper transcription factor) is activated by elevated ROS and is currently being highly researched. The Nrf2 is down regulated in prostate cancer and is important for treatment. Decreased Nrf2 means an elevated ROS and DNA damage ...
... to produce DNA damage and tumorigenesis. Nrf2(a basic-region leucine zipper transcription factor) is activated by elevated ROS and is currently being highly researched. The Nrf2 is down regulated in prostate cancer and is important for treatment. Decreased Nrf2 means an elevated ROS and DNA damage ...
The Preservation and Persistence of Human DNA in Soil during
... Thus, a clear need exists for a dedicated study of the presence and persistence of human DNA in soil, as the mere presence of human DNA in the soil can provide useful information in a number of circumstances. For example, in the event that a clandestine grave is located but is devoid of a body, the ...
... Thus, a clear need exists for a dedicated study of the presence and persistence of human DNA in soil, as the mere presence of human DNA in the soil can provide useful information in a number of circumstances. For example, in the event that a clandestine grave is located but is devoid of a body, the ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... mitochondrial genome is 16,569 base pairs (Fig. 1). Despite its small size, mtDNA encodes 22 transfer RNAs, 2 ribosomal RNAs and 13 polypeptides which are required for OXPHOS. Although the number 1000 is widely quoted as the mtDNA complement of a typical cell, there is in fact considerable variation ...
... mitochondrial genome is 16,569 base pairs (Fig. 1). Despite its small size, mtDNA encodes 22 transfer RNAs, 2 ribosomal RNAs and 13 polypeptides which are required for OXPHOS. Although the number 1000 is widely quoted as the mtDNA complement of a typical cell, there is in fact considerable variation ...
Use of novel assays to measure in vivo base excision DNA repair
... oxidized (Nilsen & Kroken, 2001). If left unrepaired, oxidized bases lead to apurinic/apyrimidic (AP) sites and strand breaks (Friedberg et al., 2006a, chap. 2), and result in DNA mutations, genetic instability, and cancer. Oxidized bases and AP sites are mainly repaired using the BER pathway (Seebe ...
... oxidized (Nilsen & Kroken, 2001). If left unrepaired, oxidized bases lead to apurinic/apyrimidic (AP) sites and strand breaks (Friedberg et al., 2006a, chap. 2), and result in DNA mutations, genetic instability, and cancer. Oxidized bases and AP sites are mainly repaired using the BER pathway (Seebe ...
Molecular events during translocation and proofreading extracted
... single-stranded DNA template after a correct incorporation. Third, if an incorrect nucleotide is accidentally incorporated into the primer, the 3 terminus of the primer is switched to the exonuclease active site more than 30 Å away for an excision. When the corrected primer returns back to the pol ...
... single-stranded DNA template after a correct incorporation. Third, if an incorrect nucleotide is accidentally incorporated into the primer, the 3 terminus of the primer is switched to the exonuclease active site more than 30 Å away for an excision. When the corrected primer returns back to the pol ...
Solving a Crime Using DNA Analysis and Chemistry
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
IS1245 restriction fragment length polymorphism typing - HAL
... isolates from a wide variety of bird species were found to possess identical three-band patterns (2, 18). The three-band pattern found in birds was also found in a small fraction of the pig isolates. As this pattern was only rarely encountered among human isolates, birds were found not to be an impo ...
... isolates from a wide variety of bird species were found to possess identical three-band patterns (2, 18). The three-band pattern found in birds was also found in a small fraction of the pig isolates. As this pattern was only rarely encountered among human isolates, birds were found not to be an impo ...
as PDF
... cyanol and Bromophenol blue are the two common dyes used as loading buffers and they run about the same speed as DNA fragments that are 5000 bp and 300 bp respectively. The other less frequently used progress markers are Cresol Red and Orange G which run at about 125 bp and 50 bp, respectively. If s ...
... cyanol and Bromophenol blue are the two common dyes used as loading buffers and they run about the same speed as DNA fragments that are 5000 bp and 300 bp respectively. The other less frequently used progress markers are Cresol Red and Orange G which run at about 125 bp and 50 bp, respectively. If s ...
Directions for Use Uracil-DNA Glycosylase (UNG), Cod
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ...
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ...
Unraveling DNA helicases
... The ADP moiety is located at the bottom of the cleft between subdomains 1A and 2A. However, only 1A can bind ADP. This cleft is lined with conserved helicase motifs. No site-bound Mg2+ has been found in the two crystal structures. On the basis of structural information for PcrA, a possible mechanism ...
... The ADP moiety is located at the bottom of the cleft between subdomains 1A and 2A. However, only 1A can bind ADP. This cleft is lined with conserved helicase motifs. No site-bound Mg2+ has been found in the two crystal structures. On the basis of structural information for PcrA, a possible mechanism ...
the mass spectrometry-based method EpiTYPER
... Select a tab delimited text file with the genomic sequence of the designed amplicon. The function will write several output files in the directory of this tab delimited file. Repeat this this step for each amplicon. 21. In each of the directories of the tab delimited text file with the amplicon sequ ...
... Select a tab delimited text file with the genomic sequence of the designed amplicon. The function will write several output files in the directory of this tab delimited file. Repeat this this step for each amplicon. 21. In each of the directories of the tab delimited text file with the amplicon sequ ...
Alu electrophoresis PCR lab
... electrical current and a gel matrix to separate molecules like DNA and proteins. ...
... electrical current and a gel matrix to separate molecules like DNA and proteins. ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.