Principles and challenges of genome
... frequently in the genome but relatively rarely within CpG islands56. This digestion step is often incorporated into protocols to deplete the sample of CpG-poor DNA before further processing, such as adaptor ligation. small genomes, such as the A. thaliana genome, do not require an amplification step ...
... frequently in the genome but relatively rarely within CpG islands56. This digestion step is often incorporated into protocols to deplete the sample of CpG-poor DNA before further processing, such as adaptor ligation. small genomes, such as the A. thaliana genome, do not require an amplification step ...
CHK2 kinase: cancer susceptibility and cancer therapy – two sides
... both CHK2 and the functionally related CHK1 kinase can be activated by the ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) kinases. These two kinases act as transducers of the DNA-damage signal as they are downstream of specific complexes of protein sensors that detect the insult ...
... both CHK2 and the functionally related CHK1 kinase can be activated by the ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) kinases. These two kinases act as transducers of the DNA-damage signal as they are downstream of specific complexes of protein sensors that detect the insult ...
DNAse I Qualification and Sample Treatment | Molecular Devices
... The Threshold Total DNA Assay quantitates contaminant DNA in biopharmaceutical drugs. DNase I can be used in conjunction with the Total DNA Assay for two purposes: To validate that the assay signal and subsequent quantitation generated by the Total DNA Assay specifically measures DNA and not a non-DN ...
... The Threshold Total DNA Assay quantitates contaminant DNA in biopharmaceutical drugs. DNase I can be used in conjunction with the Total DNA Assay for two purposes: To validate that the assay signal and subsequent quantitation generated by the Total DNA Assay specifically measures DNA and not a non-DN ...
RHD - Labex
... samples was performed using the Free Fetal DNA Kit® RhD (by using exon 5, 7 and 10) ...
... samples was performed using the Free Fetal DNA Kit® RhD (by using exon 5, 7 and 10) ...
Cellular functions of the BRCA tumour
... DNA-damaging agents led to the hypothesis that both genes function in the maintenance of genome stability, potentially through a direct role in DNA repair. This possibility received further support by the findings that BRCA1 and BRCA2 foci co-localize with the DNA-repair protein Rad51 that is essent ...
... DNA-damaging agents led to the hypothesis that both genes function in the maintenance of genome stability, potentially through a direct role in DNA repair. This possibility received further support by the findings that BRCA1 and BRCA2 foci co-localize with the DNA-repair protein Rad51 that is essent ...
J Urol - prostata
... Policlinico A. Gemelli, Largo A. Gemelli, 8, 00168 Roma, Italy. [email protected] Biologic factors affect the ability of radiation to effectively treat all patients with prostate cancer. The use of prognostic and genetic markers (12 lipoxygenase, p53, bc1-2 genes, ploidy) may aid in the develop ...
... Policlinico A. Gemelli, Largo A. Gemelli, 8, 00168 Roma, Italy. [email protected] Biologic factors affect the ability of radiation to effectively treat all patients with prostate cancer. The use of prognostic and genetic markers (12 lipoxygenase, p53, bc1-2 genes, ploidy) may aid in the develop ...
No Slide Title
... Cyclin-cdks are themselves regulated by phosphorylation of the cdk • Cyclin associated cdk is still inactive as a kinase • Threonine at position 160 (T160) of cdk2 has to be phosphorylated for the kinase to be active. The cdk activating kinase (CAK) is actually cyclin H-cdk7 • Threonine at position ...
... Cyclin-cdks are themselves regulated by phosphorylation of the cdk • Cyclin associated cdk is still inactive as a kinase • Threonine at position 160 (T160) of cdk2 has to be phosphorylated for the kinase to be active. The cdk activating kinase (CAK) is actually cyclin H-cdk7 • Threonine at position ...
DNA methylation controls histone H3 lysine 9 methylation
... Fig. 2. Location of repetitive and single-copy sequences in leaf interphase nuclei. (A) Sequences corresponding to the 180 bp centromeric pAL repeat (red) are always located at chromocenters. Sequences corresponding to the pericentromeric BAC F28D6 (green) are located at chromocenters in wild type, ...
... Fig. 2. Location of repetitive and single-copy sequences in leaf interphase nuclei. (A) Sequences corresponding to the 180 bp centromeric pAL repeat (red) are always located at chromocenters. Sequences corresponding to the pericentromeric BAC F28D6 (green) are located at chromocenters in wild type, ...
Illustrating Python via Bioinformatics Examples
... Timings on a MacBook Air 11 running Ubuntu show that the functions using list.append require almost the double of the time of the functions that work with list comprehensions. Even faster is the simple iteration over the string. However, the built-in count functionality of strings (dna.count(base)) ...
... Timings on a MacBook Air 11 running Ubuntu show that the functions using list.append require almost the double of the time of the functions that work with list comprehensions. Even faster is the simple iteration over the string. However, the built-in count functionality of strings (dna.count(base)) ...
bacterial plasmids - Acta Medica Medianae
... every single observed strain. The structure of plasmids is made of circular double chain DNA molecules which are replicated autonomously in a host cell. Their length may vary from few up to several hundred kilobase (kb). Among the bacteria, plasmids are mostly transferred horizontally by conjugation ...
... every single observed strain. The structure of plasmids is made of circular double chain DNA molecules which are replicated autonomously in a host cell. Their length may vary from few up to several hundred kilobase (kb). Among the bacteria, plasmids are mostly transferred horizontally by conjugation ...
the association of chloroplast dna with photosynthetic membrane
... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
Developmental Validation of the Quantifiler Real-Time
... a 3 non-fluorescent quencher, that is homologous to the amplicon region between the PCR primers. The TaqMan probes used in the Quantifiler kits incorporate an additional chemical modification at their 3 ends, known as minor groove binder (MGB), to increase the melting temperature (Tm ) and thereby ...
... a 3 non-fluorescent quencher, that is homologous to the amplicon region between the PCR primers. The TaqMan probes used in the Quantifiler kits incorporate an additional chemical modification at their 3 ends, known as minor groove binder (MGB), to increase the melting temperature (Tm ) and thereby ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
Structural basis of PAM-dependent target DNA recognition by the
... domains1,2,7. Cas9 has been exploited in numerous gene-targeting applications, in which its sequence specificity is programmed by either dual crRNA–tracrRNA guides or chimaeric single-molecule guide RNAs (sgRNAs)8–19. PAM recognition is a critical aspect of Cas9-mediated DNA targeting, being a prere ...
... domains1,2,7. Cas9 has been exploited in numerous gene-targeting applications, in which its sequence specificity is programmed by either dual crRNA–tracrRNA guides or chimaeric single-molecule guide RNAs (sgRNAs)8–19. PAM recognition is a critical aspect of Cas9-mediated DNA targeting, being a prere ...
Synthesis and Evaluation of Guanidino Phthalocyanines for G
... strongly suggesting a regulatory role in vivo. In vitro studies have already shown that these repeats can fold into triple helix-containing structures called H-DNA. Upon H-DNA formation, the DNA double helix within one-half of the H-palindrome denatures into two single strands, and one of these comp ...
... strongly suggesting a regulatory role in vivo. In vitro studies have already shown that these repeats can fold into triple helix-containing structures called H-DNA. Upon H-DNA formation, the DNA double helix within one-half of the H-palindrome denatures into two single strands, and one of these comp ...
p53-associated 3 exonuclease activity in nuclear and cytoplasmic
... exonuclease activity of p53 must not be restricted to its noninduced state, but might also be exerted by a subclass of p53 after DNA damage when the protein is able to display its full range of possible biochemical activities (Albrechtsen et al., 1999). p53 was found in the nucleus, the cytoplasm or ...
... exonuclease activity of p53 must not be restricted to its noninduced state, but might also be exerted by a subclass of p53 after DNA damage when the protein is able to display its full range of possible biochemical activities (Albrechtsen et al., 1999). p53 was found in the nucleus, the cytoplasm or ...
Full-Text PDF
... ubiquitin-protein ligase APCCdc20. Cdt1 proteolysis and Geminin accumulation work in parallel to prevent re-licensing after initiation. Abolition of either process by itself does not measurably affect the extent of DNA replication, but if both processes are inactivated simultaneously then the genome ...
... ubiquitin-protein ligase APCCdc20. Cdt1 proteolysis and Geminin accumulation work in parallel to prevent re-licensing after initiation. Abolition of either process by itself does not measurably affect the extent of DNA replication, but if both processes are inactivated simultaneously then the genome ...
Power Point
... If two individuals with two pairs of heterozygous genes (each affecting a different trait) are mated, the expected genotypic and phenotypic ratios would be: ...
... If two individuals with two pairs of heterozygous genes (each affecting a different trait) are mated, the expected genotypic and phenotypic ratios would be: ...
animal genetics
... If two individuals with two pairs of heterozygous genes (each affecting a different trait) are mated, the expected genotypic and phenotypic ratios would be: ...
... If two individuals with two pairs of heterozygous genes (each affecting a different trait) are mated, the expected genotypic and phenotypic ratios would be: ...
Collaborative coupling between polymerase and helicase for
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
Chpt8_RecombineDNA.doc
... general recombination. Illegitimate or nonhomologous recombination occurs in regions where no large-scale sequence similarity is apparent, e.g. translocations between different chromosomes or deletions that remove several genes along a chromosome. However, when the DNA sequence at the breakpoints fo ...
... general recombination. Illegitimate or nonhomologous recombination occurs in regions where no large-scale sequence similarity is apparent, e.g. translocations between different chromosomes or deletions that remove several genes along a chromosome. However, when the DNA sequence at the breakpoints fo ...
Escherichia coli DNA ligase B may mitigate damage from
... Escherichia coli encodes two DNA ligases, ligase A, which is essential under normal laboratory growth conditions, and ligase B, which is not. Here we report potential functions of ligase B. We found that across the entire Enterobacteriaceae family, ligase B is highly conserved in both amino acid ide ...
... Escherichia coli encodes two DNA ligases, ligase A, which is essential under normal laboratory growth conditions, and ligase B, which is not. Here we report potential functions of ligase B. We found that across the entire Enterobacteriaceae family, ligase B is highly conserved in both amino acid ide ...
The Isolation of Mutagen-Sensitive nuv Mutants of
... 1988, 1991). Work with filamentous fungi has progressed much more slowly such that only a relatively small number of such mutants have been isolated and characterized. In Aspergillus nidulans only nine UV-sensitive (uvs) mutants have previously been extensively characterizedfor effects onrecombinati ...
... 1988, 1991). Work with filamentous fungi has progressed much more slowly such that only a relatively small number of such mutants have been isolated and characterized. In Aspergillus nidulans only nine UV-sensitive (uvs) mutants have previously been extensively characterizedfor effects onrecombinati ...
2 - cellbiochem.ca
... bacterial DNA polymerase to bind and initiate DNA replication; • Several Ori can be found. ColE1 is the most frequently encountered; • Some Ori allow the plasmid to replicate frequently (high copy number plasmids – up to 100 copies per cell); others allow only a low rate of replication initiation (l ...
... bacterial DNA polymerase to bind and initiate DNA replication; • Several Ori can be found. ColE1 is the most frequently encountered; • Some Ori allow the plasmid to replicate frequently (high copy number plasmids – up to 100 copies per cell); others allow only a low rate of replication initiation (l ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.