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Genetics Objectives Unit VII, Biotechnology 1. Define biotechnology, Taq1, sticky ends, vector, plasmid, bioremediation, restriction enzyme, bioinformatics, and genomics. 2. What is meant by the universality of the genetic code? 3. Define polymerase chain reaction (PCR). Describe what is needed for PCR to happen, its process of DNA amplification, and list several uses. 4. Describe the process of gel electrophoresis and how it is used. 5. Define and describe DNA sequencing. Be able to read a DNA sequence from an autoradiogram. 6. Describe the process of using recombinant DNA technology to make a transgenic plant or animal. What are the advantages and disadvantages of these plants and animals? 7. List 2 examples each of transgenic plants and transgenic animals. 8. What was the first human drug marketed using DNA recombinant technology? What are the advantages of producing drugs this way? 9. Describe how the BLAST computer program is used in genetic research. 10. What is the job description of a genetic counselor? 11. What is gene therapy? 12. Give a brief summary of the stories of Ashi DeSilva, Jessie Gelsinger, and Max Randell. 13. When did the human genone project start, when was it completed, and what government agencies were involved. What type of chromosome mapping was used? 14. Describe the 4 types of chromosome maps: cytogenetic, linkage, physical, and sequence. Reading Ch. 19, all Ch. 20, all Ch. 22 (22.1 and 22.2 only) Labs CSI Lab, Museum of Science and Industry Webcutter Lab with BLAST Beta Globin Gene Exercise Lab