Cleavage Close to the End of DNA Fragments (linearized
... determined by dividing the number of transformants from the digestion reaction by the number obtained from religation of the linearized DNA (typically 100–500 colonies) and subtracting from 100%. “Base Pairs from End” refers to the number of double-stranded base pairs between the end of the recognit ...
... determined by dividing the number of transformants from the digestion reaction by the number obtained from religation of the linearized DNA (typically 100–500 colonies) and subtracting from 100%. “Base Pairs from End” refers to the number of double-stranded base pairs between the end of the recognit ...
Synthesis and Biophysical Studies of Nucleic Acid-Binding
... phosphoester linkage. The most substantial difference between DNA and RNA is the nature of the sugar unit. DNA contains a 2-deoxyribose moiety as opposed to the ribose in RNA. Thus, RNA can be distinguished chemically from DNA by its greater instability in alkaline solutions owing to the potential f ...
... phosphoester linkage. The most substantial difference between DNA and RNA is the nature of the sugar unit. DNA contains a 2-deoxyribose moiety as opposed to the ribose in RNA. Thus, RNA can be distinguished chemically from DNA by its greater instability in alkaline solutions owing to the potential f ...
Volume 16 - American Academy of Forensic Sciences
... Throughout the past twelve years the Young Forensic Scientists Forum has provided a program for a group of attendees ranging from students to professionals new to their career in forensic science. The program has grown and continues to change each year since its inception to provide students and sci ...
... Throughout the past twelve years the Young Forensic Scientists Forum has provided a program for a group of attendees ranging from students to professionals new to their career in forensic science. The program has grown and continues to change each year since its inception to provide students and sci ...
TaqMan® SNP Genotyping Assays User Guide
... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
Unipro UGENE Manual
... external tool needs a corresponding executable file. The list of the external tools can be found on this page. The standard package does not include the tools, whereas the full package include all the required tools. The NGS package, besides containing the external tools, contains sample data for th ...
... external tool needs a corresponding executable file. The list of the external tools can be found on this page. The standard package does not include the tools, whereas the full package include all the required tools. The NGS package, besides containing the external tools, contains sample data for th ...
Peptide nucleic acids 2
... 5. PNA as a Potential Antisense and Antigene Drug . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.1 Transcription arrest by PNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.2 P-Loops as artificial transcription promoters . . . . . . . . . . . . . . . . . . . . ...
... 5. PNA as a Potential Antisense and Antigene Drug . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.1 Transcription arrest by PNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.2 P-Loops as artificial transcription promoters . . . . . . . . . . . . . . . . . . . . ...
Introduction to Forensic Sciences
... of the conclusions drawn from the investigation by responsible authorities. A few attempts were made to organize special areas within police departments for processing evidence. Investigating authorities individually obtained scientific information from academic departments of chemistry or pharmacol ...
... of the conclusions drawn from the investigation by responsible authorities. A few attempts were made to organize special areas within police departments for processing evidence. Investigating authorities individually obtained scientific information from academic departments of chemistry or pharmacol ...
Forensic Science - ACS Publications
... The developmental validation for Promega Corporation’s Plexor HY quantitative PCR assay was published (21), as was Applied Biosystems’ Quantifiler Duo assay (22). Quantitation of the amount of nuclear DNA in human telogen hairs was reported (23). Reduced volume Quantifiler assays were validated (24) ...
... The developmental validation for Promega Corporation’s Plexor HY quantitative PCR assay was published (21), as was Applied Biosystems’ Quantifiler Duo assay (22). Quantitation of the amount of nuclear DNA in human telogen hairs was reported (23). Reduced volume Quantifiler assays were validated (24) ...
HIV-1 Reverse Transcriptase-associated
... The reverse transcriptase (RT) of HIV-1 contains two active sites, polymerase and ribonuclease (RNase) H that are both absolutely necessary for viral replication and disease progression. While several drugs currently used in the clinic to treat HIV-1 target the polymerase activity of RT, none curren ...
... The reverse transcriptase (RT) of HIV-1 contains two active sites, polymerase and ribonuclease (RNase) H that are both absolutely necessary for viral replication and disease progression. While several drugs currently used in the clinic to treat HIV-1 target the polymerase activity of RT, none curren ...
The presence of an RHD pseudogene containing a
... proposed that these techniques could be used for testing all D-negative pregnant women to ascertain whether they require administration of antenatal anti-D immunoglobulin.18,19 Testing would reduce wastage of this valuable resource by avoiding anti-D immunoglobulin administration to women with a D-n ...
... proposed that these techniques could be used for testing all D-negative pregnant women to ascertain whether they require administration of antenatal anti-D immunoglobulin.18,19 Testing would reduce wastage of this valuable resource by avoiding anti-D immunoglobulin administration to women with a D-n ...
Microscopic Mechanics of Hairpin DNA Translocation through
... sequence measurement. Furthermore, Nakane et al. (8) have shown that single nucleotide polymorphisms can be detected by measuring the average time required for unzipping a DNA duplex, while Soni and Meller (10) demonstrated the feasibility of an ultrafast sequencing method in which the DNA sequence ...
... sequence measurement. Furthermore, Nakane et al. (8) have shown that single nucleotide polymorphisms can be detected by measuring the average time required for unzipping a DNA duplex, while Soni and Meller (10) demonstrated the feasibility of an ultrafast sequencing method in which the DNA sequence ...
Unequal Crossing-over in Unique PABP2 Mutations in Japanese
... repeats are responsible for a number of hereditary neuromuscular diseases.7,14,15 These pathologic repeat expansions can be explained by the slippage model. However, it has been proposed that tracts of approximately 25 to 35 perfect trinucleotide repeats are required for instability and expansion vi ...
... repeats are responsible for a number of hereditary neuromuscular diseases.7,14,15 These pathologic repeat expansions can be explained by the slippage model. However, it has been proposed that tracts of approximately 25 to 35 perfect trinucleotide repeats are required for instability and expansion vi ...
Product description P018-G1 SHOX-v03 - MRC
... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
Parallel Session Abstracts
... Over the past decade, DNA barcoding has transitioned from academic battleground to major research enterprise. For the first few years, reviewers were unkind, critics were vocal, and funding was scarce. The situation has shifted; our community now oversees the largest research program ever undertaken ...
... Over the past decade, DNA barcoding has transitioned from academic battleground to major research enterprise. For the first few years, reviewers were unkind, critics were vocal, and funding was scarce. The situation has shifted; our community now oversees the largest research program ever undertaken ...
f o r e n s i c science - and
... evidence that can be collected for analysis, but may not always produce a DNA profile. Generally, all these pieces of evidence do not contain a substantial amount of biological material and are processed for DNA without going through any type of serological screening to maximize the amount of sample ...
... evidence that can be collected for analysis, but may not always produce a DNA profile. Generally, all these pieces of evidence do not contain a substantial amount of biological material and are processed for DNA without going through any type of serological screening to maximize the amount of sample ...
Redesign of the monomer–monomer interface of Cre recombinase
... The quaternary structure of the Cre complex creates a second challenge for engineering novel RT specificities. The four-fold symmetry in the functional protein complex imposes a pseudo-palindromic symmetry upon the RT site. The loxP site consists of two 13 bp palindromic half-sites separated by an a ...
... The quaternary structure of the Cre complex creates a second challenge for engineering novel RT specificities. The four-fold symmetry in the functional protein complex imposes a pseudo-palindromic symmetry upon the RT site. The loxP site consists of two 13 bp palindromic half-sites separated by an a ...
Methods for Improving Aptamer Binding Affinity
... with high affinity but, in starting libraries, the diversity of experimentally applicable oligonucleotide molecules is limited. Furthermore, aptamer structures are essential for their function, but may be adversely affected during PCR. DNA molecules that form stable structures resist PCR amplificati ...
... with high affinity but, in starting libraries, the diversity of experimentally applicable oligonucleotide molecules is limited. Furthermore, aptamer structures are essential for their function, but may be adversely affected during PCR. DNA molecules that form stable structures resist PCR amplificati ...
PDF
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
De novo DNA methylation through the 5′-segment
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.