(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real
... Salmonella species are important causes of enteritis throughout the world. In addition, Salmonella enterica serotypes Typhi and Paratyphi are important causes of enteric fever in underdeveloped countries that lack adequate sewage disposal and water treatment facilities.1,2 It is standard practice in ...
... Salmonella species are important causes of enteritis throughout the world. In addition, Salmonella enterica serotypes Typhi and Paratyphi are important causes of enteric fever in underdeveloped countries that lack adequate sewage disposal and water treatment facilities.1,2 It is standard practice in ...
Non-homologous end-joining partners in a helical dance: structural
... stabilizing DNA ligase IV at DSBs in NHEJ DNA DSBs (double-strand breaks) can be caused by ionizing radiation or toxic chemical exposure, but are also present as intermediates in V(D)J recombination and class switch recombination for antigen receptor diversity formation. Unrepaired DSBs lead to chro ...
... stabilizing DNA ligase IV at DSBs in NHEJ DNA DSBs (double-strand breaks) can be caused by ionizing radiation or toxic chemical exposure, but are also present as intermediates in V(D)J recombination and class switch recombination for antigen receptor diversity formation. Unrepaired DSBs lead to chro ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... tissue toxicity which is the treatment limiting factor in treatment of cancer with radiation: it is easy to kill the cancer with radiation, but much more difficult to not to destroy the surrounding normal tissue and thus kill the patient. Studies of radiation-induced DNA damage like those Peacocke a ...
... tissue toxicity which is the treatment limiting factor in treatment of cancer with radiation: it is easy to kill the cancer with radiation, but much more difficult to not to destroy the surrounding normal tissue and thus kill the patient. Studies of radiation-induced DNA damage like those Peacocke a ...
Analysis of the Molecular Basis of Flowering Time Variation in
... alleles are fully functional and confer very late flowering in the presence of the strong Col FLC allele. Given the identical amino acid sequence, it is likely that the three accessions Shakhdara, Kondara, and Kz-9 carry the same functional FRI allele. The F1 plants from the cross of Kz-9 to FRI(Sf2 ...
... alleles are fully functional and confer very late flowering in the presence of the strong Col FLC allele. Given the identical amino acid sequence, it is likely that the three accessions Shakhdara, Kondara, and Kz-9 carry the same functional FRI allele. The F1 plants from the cross of Kz-9 to FRI(Sf2 ...
HP1 binding to native chromatin in vitro is determined by the hinge
... from its mammalian homologues, whereas xHP1g is highly conserved. Functionally, xHP1a behaves identically to human HP1a. We observe unexpected differences between the two HP1 variants in binding native soluble chromatin, which seem to correlate with their distinct nuclear distributions in vivo. A su ...
... from its mammalian homologues, whereas xHP1g is highly conserved. Functionally, xHP1a behaves identically to human HP1a. We observe unexpected differences between the two HP1 variants in binding native soluble chromatin, which seem to correlate with their distinct nuclear distributions in vivo. A su ...
New variants of the tet(M) gene in Clostridium
... Sequence analysis revealed that except for tet(M)1, which was 100% identical to the tet(M) gene found in Streptococcus agalactiae 2603V/R (GenBank accession no. AE014233), the other alleles did not show complete identity with other tet(M) genes. The phylogenetic tree obtained by nucleotide sequence ...
... Sequence analysis revealed that except for tet(M)1, which was 100% identical to the tet(M) gene found in Streptococcus agalactiae 2603V/R (GenBank accession no. AE014233), the other alleles did not show complete identity with other tet(M) genes. The phylogenetic tree obtained by nucleotide sequence ...
Coffee B, Ikeda M, Budimirovic DB, Hjelm LN, Kaufmann WE and Warren ST: Mosaic FMR1 Deletion Causes Fragile X Syndrome and Can Lead to Molecular Misdiagnosis: A Case Report and Review of the Literature. American J of Medical Genetics Part A 146A:1358-1367 (2008).
... with normal levels of FMRP, indicating that this deletion is unrelated to the patient’s phenotype [Hegde et al., 2001]. The remaining 56 deletions fall into two distinct classes: small deletions (<10 kb) restricted to the 50 end of the gene that are due to instability of the CGG repeat and large del ...
... with normal levels of FMRP, indicating that this deletion is unrelated to the patient’s phenotype [Hegde et al., 2001]. The remaining 56 deletions fall into two distinct classes: small deletions (<10 kb) restricted to the 50 end of the gene that are due to instability of the CGG repeat and large del ...
Postconviction DNA Testing - Crime Scene Investigator Network
... of Attorney General Janet Reno. When she read about the use of DNA to exonerate someone wrongfully convicted of rape and homicide, she became concerned that others might also have been wrongly convicted. The Attorney General then directed the National Institute of Justice (NIJ) to identify how often ...
... of Attorney General Janet Reno. When she read about the use of DNA to exonerate someone wrongfully convicted of rape and homicide, she became concerned that others might also have been wrongly convicted. The Attorney General then directed the National Institute of Justice (NIJ) to identify how often ...
Alaska Scientific Crime Detection Laboratory
... Type or print legibly. This form is used to relay information which is rendered useless if the analyst cannot read it. Clearly describe the type of evidence container and the item(s) being submitted. The description and item number should match what is on the RLS. Use one item number for each item o ...
... Type or print legibly. This form is used to relay information which is rendered useless if the analyst cannot read it. Clearly describe the type of evidence container and the item(s) being submitted. The description and item number should match what is on the RLS. Use one item number for each item o ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... gene. Two probes are included for exon 9 of EPCAM (formerly known as TACSTD1), a gene located just upstream of MSH2. Deletions of this last exon of EPCAM can result in silencing of the MSH2 gene. This probemix contains nine quality control fragments generating amplification products between 64 and 1 ...
... gene. Two probes are included for exon 9 of EPCAM (formerly known as TACSTD1), a gene located just upstream of MSH2. Deletions of this last exon of EPCAM can result in silencing of the MSH2 gene. This probemix contains nine quality control fragments generating amplification products between 64 and 1 ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... acceptors on DNA, which was achieved by bending the DNA over spermine, a process that encases the polyamine in a deepened, narrowed, and highly electronegative major groove. Thus there are considerable data that support specific interactions of polyamines with nucleic acids. More importantly, when p ...
... acceptors on DNA, which was achieved by bending the DNA over spermine, a process that encases the polyamine in a deepened, narrowed, and highly electronegative major groove. Thus there are considerable data that support specific interactions of polyamines with nucleic acids. More importantly, when p ...
Human DNA Ligase IV and the Ligase IV/XRCC4 Complex: Analysis
... of termini (blunt, sticky, compatible, and incompatible) and then sequencing the rejoined products, both the activities employed during NHEJ and the order in which these activities are utilized were previously surmised. Importantly, though a particular cell type will typically repair a given set of ...
... of termini (blunt, sticky, compatible, and incompatible) and then sequencing the rejoined products, both the activities employed during NHEJ and the order in which these activities are utilized were previously surmised. Importantly, though a particular cell type will typically repair a given set of ...
Comparison of different PCR tests for detecting Shiga toxin
... SYBR Green I or TaqMan assay is a powerful technique. Real-time PCR techniques provide results available immediately after completion of the amplification reaction, with no need for any further processing of the samples and without opening the test tubes, greatly reducing the risk of carry-over cont ...
... SYBR Green I or TaqMan assay is a powerful technique. Real-time PCR techniques provide results available immediately after completion of the amplification reaction, with no need for any further processing of the samples and without opening the test tubes, greatly reducing the risk of carry-over cont ...
Forensic Sciences - Manitoba Education and Training
... line then turn away, and the students in the other line change one thing about their appearance (for example, untie shoe, remove earring, flip hair). The students in the first line then turn back and determine what is different. • Someone runs quickly (and without warning) through the room. Students ...
... line then turn away, and the students in the other line change one thing about their appearance (for example, untie shoe, remove earring, flip hair). The students in the first line then turn back and determine what is different. • Someone runs quickly (and without warning) through the room. Students ...
24 Recombination Hotspots in Nonallelic Homologous Recombination
... the fine-mapping of all homologous recombination (HR) processes can only help our admittedly basic comprehension of what is a fundamental cellular process. In recent years there has been a growing desire to be able to use patterns of linkage disequilibrium (LD) throughout the genome to design more e ...
... the fine-mapping of all homologous recombination (HR) processes can only help our admittedly basic comprehension of what is a fundamental cellular process. In recent years there has been a growing desire to be able to use patterns of linkage disequilibrium (LD) throughout the genome to design more e ...
Agarose Gel Electrophoresis: Best Practices
... Optimimal separation of the fragments is influenced by best practices followed upon the three steps of the workflow in agarose gel electrophoresis: ...
... Optimimal separation of the fragments is influenced by best practices followed upon the three steps of the workflow in agarose gel electrophoresis: ...
Group I and Group II Ribozymes as RNPs: Clues to the
... evolution of their protein-assisted splicing reactions. For example, the splicing factors for both types of introns include intron-encoded as well as cellular proteins, and the intron-encoded proteins, DNA endonucleases for group I introns and reverse transcriptases (RTs) for group II introns, also ...
... evolution of their protein-assisted splicing reactions. For example, the splicing factors for both types of introns include intron-encoded as well as cellular proteins, and the intron-encoded proteins, DNA endonucleases for group I introns and reverse transcriptases (RTs) for group II introns, also ...
Designing synthetic MLPA probes - MRC
... http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?taxid=9606 Sequence between start and stop codon. In Map Viewer, numbers mentioned under CDS refer to numbering of the Genbank entry of NM sequence. Most of our probes are located in coding regions of genes. We use the term Forward Probe when the hy ...
... http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?taxid=9606 Sequence between start and stop codon. In Map Viewer, numbers mentioned under CDS refer to numbering of the Genbank entry of NM sequence. Most of our probes are located in coding regions of genes. We use the term Forward Probe when the hy ...
Two Components of the RNA-Directed DNA Methylation Pathway
... forming a heteromer required for RdDM [23–26]. Pol V-produced scaffold RNAs are bound not only by AGO4 but also by IDN2 and DRM2. AGO4 and IDN2 are required for the binding of DRM2 to Pol V-produced scaffold RNAs [27]. KTF1/SPT5L functions together with AGO4 and acts as an effector in the RdDM pathw ...
... forming a heteromer required for RdDM [23–26]. Pol V-produced scaffold RNAs are bound not only by AGO4 but also by IDN2 and DRM2. AGO4 and IDN2 are required for the binding of DRM2 to Pol V-produced scaffold RNAs [27]. KTF1/SPT5L functions together with AGO4 and acts as an effector in the RdDM pathw ...
Turning h Cro into a Transcriptional Activator
... more tightly than to OR2, while wild-type h Cro binds equally tightly to 0~1 and 0~2. In order to facilitate assays of activation by Cro67, we introduced four base changes into OR2 that make its sequence more like that of 0~1 (Figure 3); Figure 4 shows that Cro67 bound to 0~1 and the modified OR2 eq ...
... more tightly than to OR2, while wild-type h Cro binds equally tightly to 0~1 and 0~2. In order to facilitate assays of activation by Cro67, we introduced four base changes into OR2 that make its sequence more like that of 0~1 (Figure 3); Figure 4 shows that Cro67 bound to 0~1 and the modified OR2 eq ...
PCR and qPCR product guide
... extension steps of PCR, and thus fresh enzyme was not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s first “molecule of the year” in 19895. Although a vast improvement over using E. coli Pol I, Taq po ...
... extension steps of PCR, and thus fresh enzyme was not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s first “molecule of the year” in 19895. Although a vast improvement over using E. coli Pol I, Taq po ...
Formosa haliotis sp. nov., a brown-alga
... F. algae LMG 28216T and F. arctica LMG 28318T were used as reference strains for DNA–DNA hybridizations, analyses of fatty acids and phenotypic characterization. F. algae LMG 28216T was also included in random amplified polymorphic DNA (RAPD) analysis. Genomic DNA for RAPD, DNA–DNA hybridizations an ...
... F. algae LMG 28216T and F. arctica LMG 28318T were used as reference strains for DNA–DNA hybridizations, analyses of fatty acids and phenotypic characterization. F. algae LMG 28216T was also included in random amplified polymorphic DNA (RAPD) analysis. Genomic DNA for RAPD, DNA–DNA hybridizations an ...
Critical Factors for Successful Real-Time PCR
... In multiplex, real-time PCR, several genomic DNA targets are quantified simultaneously in the same reaction. Multiplex, real-time RT-PCR is a similar method, allowing simultaneous quantification of several RNA targets in the same reaction. The procedure can be performed either as two-step RT-PCR or ...
... In multiplex, real-time PCR, several genomic DNA targets are quantified simultaneously in the same reaction. Multiplex, real-time RT-PCR is a similar method, allowing simultaneous quantification of several RNA targets in the same reaction. The procedure can be performed either as two-step RT-PCR or ...
Current Topics LNA (Locked Nucleic Acid): High
... indeed very promising for a future development of LNAzymes for use in cells and for in ViVo applications. LNAzymes enhance hybridization, allowing them to access RNA structures not targeted by the corresponding DNAzymes; they show enhanced nuclease stability that increases the half-life in cells, an ...
... indeed very promising for a future development of LNAzymes for use in cells and for in ViVo applications. LNAzymes enhance hybridization, allowing them to access RNA structures not targeted by the corresponding DNAzymes; they show enhanced nuclease stability that increases the half-life in cells, an ...
Lecture 1. Introduction
... Large data size • The last page contains about 500 characters – Need 6,000,000 pages to show the human genome – Printed in 130 books ...
... Large data size • The last page contains about 500 characters – Need 6,000,000 pages to show the human genome – Printed in 130 books ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.