`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... Recent investigations, particularly sequence analysis of 16S rDNA, have revealed that phytoplasmas constitute a coherent, genus-level taxon. In the monophyletic phytoplasma clade, groups and subgroups have been delineated, many of which are being considered as putative species under the provisional ...
... Recent investigations, particularly sequence analysis of 16S rDNA, have revealed that phytoplasmas constitute a coherent, genus-level taxon. In the monophyletic phytoplasma clade, groups and subgroups have been delineated, many of which are being considered as putative species under the provisional ...
Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene
... cashmere fibre diameter; in long-term artificial fertilization and selection the excellent trait is always primarily preserved. The sequencing results showed that two unreported mutations, A214G and T218C were identified in the KAP8.2 gene. A214G did not change the amino acid, while T218C led to an ...
... cashmere fibre diameter; in long-term artificial fertilization and selection the excellent trait is always primarily preserved. The sequencing results showed that two unreported mutations, A214G and T218C were identified in the KAP8.2 gene. A214G did not change the amino acid, while T218C led to an ...
CC 1 070-71..8171c ose chapter .. Page70
... triplex melts at 32 °C. In the presence of 4 mM neomycin and 4 mM pyrene–neomycin, triplex Tm rises to 43 and 58 °C, respectively. These results show that pyrene–neomycin can stabilize the triplex much more effectively than neomycin at these low concentrations (rdb = 0.26). This is quite remarkable ...
... triplex melts at 32 °C. In the presence of 4 mM neomycin and 4 mM pyrene–neomycin, triplex Tm rises to 43 and 58 °C, respectively. These results show that pyrene–neomycin can stabilize the triplex much more effectively than neomycin at these low concentrations (rdb = 0.26). This is quite remarkable ...
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... cysteine desulfurase, which has a similar function as DndA was then identified in E. coli to support DNA phosphorothioation [10,11]. dnd operons are often located in chromosomal islands, where highly diverse genetic contexts were observed across different bacterial species [10]. However, there is de ...
... cysteine desulfurase, which has a similar function as DndA was then identified in E. coli to support DNA phosphorothioation [10,11]. dnd operons are often located in chromosomal islands, where highly diverse genetic contexts were observed across different bacterial species [10]. However, there is de ...
MART TOOTS Novel Means to Target Human Papillomavirus Infection
... target of interest rapidly. As part of this study, we have established this type of cellular system to monitor the genome replication of different HPVs. Using this system allowed us to identify several new compounds that specifically inhibit the replication of oncogenic high-risk HPVs. ...
... target of interest rapidly. As part of this study, we have established this type of cellular system to monitor the genome replication of different HPVs. Using this system allowed us to identify several new compounds that specifically inhibit the replication of oncogenic high-risk HPVs. ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... instance, purines are larger and thus one might expect that they would interact more strongly with surfaces in a confined space. The backbone structure of a polynucleotide is shown in Fig. 3a. Each monomer unit is a nucleotide, which consists of a base, phosphate group (PO4 ), and sugar. For DNA (RN ...
... instance, purines are larger and thus one might expect that they would interact more strongly with surfaces in a confined space. The backbone structure of a polynucleotide is shown in Fig. 3a. Each monomer unit is a nucleotide, which consists of a base, phosphate group (PO4 ), and sugar. For DNA (RN ...
O A RIGINAL RTICLE
... tried to find a rapid, sensitive and specific method of detecting such bacterium in water. Alike other developments, bacterial detection methods are developing through the time, it means the first methods were biochemical (enzymatic) while nowadays that's the molecular (genetic) ones which override ...
... tried to find a rapid, sensitive and specific method of detecting such bacterium in water. Alike other developments, bacterial detection methods are developing through the time, it means the first methods were biochemical (enzymatic) while nowadays that's the molecular (genetic) ones which override ...
Identity of zinc finger nucleases with specificity novel HSV-2 vaccine/therapy precursors
... complete sequenced genome of a strain of HSV-2, HG52 (Dolan et al. [27]), reveals that HSV-2 genomic DNA is organized into two unique regions of double-stranded DNA (long 126-kb and short 26-kb short) denoted as UL and US. These are bracketed by inverted repeat sequences (TRL-IRL and IRS-TRS) that r ...
... complete sequenced genome of a strain of HSV-2, HG52 (Dolan et al. [27]), reveals that HSV-2 genomic DNA is organized into two unique regions of double-stranded DNA (long 126-kb and short 26-kb short) denoted as UL and US. These are bracketed by inverted repeat sequences (TRL-IRL and IRS-TRS) that r ...
Presence of multiple group I introns closely 23S rRNAs of lichen-forming
... subgroup introns featured the loop of the P8 helix; the IA3 and IB4 subgroups featured an extension from the peripheral loops towards the intron core structure. More importantly, all of these putative proteins contained one LAGLIDADG motif, identified by using Pfam v21.10 [17], characteristic of the ...
... subgroup introns featured the loop of the P8 helix; the IA3 and IB4 subgroups featured an extension from the peripheral loops towards the intron core structure. More importantly, all of these putative proteins contained one LAGLIDADG motif, identified by using Pfam v21.10 [17], characteristic of the ...
Tomé, S., Manley, K., Simard, J.P., Clark, G.W., Slean, M.M., Swami
... MutSb is required to drive CAG expansions [13,27,35] and to protect against repeat contractions [16,26,27,34–36,49]. The role of MutSb in expansions extends beyond its ability to bind slippedDNAs [50] as an ATPase-functional MutSb complex is necessary for CAG expansions [47] and downstream mismatch ...
... MutSb is required to drive CAG expansions [13,27,35] and to protect against repeat contractions [16,26,27,34–36,49]. The role of MutSb in expansions extends beyond its ability to bind slippedDNAs [50] as an ATPase-functional MutSb complex is necessary for CAG expansions [47] and downstream mismatch ...
Leuconostoc miyukkimchii sp. nov., isolated from brown algae
... Research project (CIPRES, www.phylo.org; Stamatakis et al., 2005) at the San Diego Supercomputer Center. To study the relatedness between strain M2T and its neighbours in the genus Leuconostoc with a higher resolution than that provided by 16S rRNA gene sequences, comparative sequence analysis of th ...
... Research project (CIPRES, www.phylo.org; Stamatakis et al., 2005) at the San Diego Supercomputer Center. To study the relatedness between strain M2T and its neighbours in the genus Leuconostoc with a higher resolution than that provided by 16S rRNA gene sequences, comparative sequence analysis of th ...
The RNAs of RNA-directed DNA methylation
... CMT2, which maintain symmetric CHG methylation (CMT3) or maintain asymmetric CHH methylation in regions of dense pericentric heterochromatin (CMT2) [11,12,87,100]. Proteins that recognize the different DNA methylation patterns help recruit chromatin modifying enzymes that chemically modify the assoc ...
... CMT2, which maintain symmetric CHG methylation (CMT3) or maintain asymmetric CHH methylation in regions of dense pericentric heterochromatin (CMT2) [11,12,87,100]. Proteins that recognize the different DNA methylation patterns help recruit chromatin modifying enzymes that chemically modify the assoc ...
Spinocerebellar ataxia type 1 (SCA1): Phenotype-genotype
... Sequence analysis revealed that the 14 normal SCA1 alleles with 29 and 30 triplets as well as the alleles with 35 ± 38 triplets are interrupted by the sequence CAT CAG CAT. In alleles with 39 ± 41 triplets the interruption is missing in six of seven cases. DNA sequences are summarised in Table 1. Al ...
... Sequence analysis revealed that the 14 normal SCA1 alleles with 29 and 30 triplets as well as the alleles with 35 ± 38 triplets are interrupted by the sequence CAT CAG CAT. In alleles with 39 ± 41 triplets the interruption is missing in six of seven cases. DNA sequences are summarised in Table 1. Al ...
Identification and isolation of active N2O reducers in rice paddy soil
... the 16S rRNA and functional gene diversities of microbial populations involved in specific functions. Previously, 13C-assimilating populations under denitrifying conditions were analyzed by DNA-based SIP analysis (Ginige et al., 2004; Osaka et al., 2006; Osaka et al., 2008; Saito et al., 2008). Howe ...
... the 16S rRNA and functional gene diversities of microbial populations involved in specific functions. Previously, 13C-assimilating populations under denitrifying conditions were analyzed by DNA-based SIP analysis (Ginige et al., 2004; Osaka et al., 2006; Osaka et al., 2008; Saito et al., 2008). Howe ...
An Introduction to Peptide Nucleic Acid
... The field of life science realized early on the important implications of these traits. If specific, single strands of DNA could be synthesized, then the base sequences of genes could be studied and manipulated using these defined molecules. With the advent of efficient chemistries for DNA/RNA synth ...
... The field of life science realized early on the important implications of these traits. If specific, single strands of DNA could be synthesized, then the base sequences of genes could be studied and manipulated using these defined molecules. With the advent of efficient chemistries for DNA/RNA synth ...
a method for detecting and typing of salmonella by multiplex pcr
... selling ready-made food is growing throughout the country. The veterinary service should have time to check the quality of all of these products. Only traditional bacteriological methods of isolation and identification of pathogens of toxicoinfection, which is not enough in terms of increasing turno ...
... selling ready-made food is growing throughout the country. The veterinary service should have time to check the quality of all of these products. Only traditional bacteriological methods of isolation and identification of pathogens of toxicoinfection, which is not enough in terms of increasing turno ...
Proof-of-principle rapid noninvasive prenatal diagnosis
... SNP panel. Six of these homoallelic patients with type I Gaucher disease were homozygotic for all 490 SNPs on our initial sequencing panel. The seventh sample shared the same haplotype within and 3′ to GBA, but a heterozygous region was clearly identified 144,388 nucleotides 5′ to the gene and beyon ...
... SNP panel. Six of these homoallelic patients with type I Gaucher disease were homozygotic for all 490 SNPs on our initial sequencing panel. The seventh sample shared the same haplotype within and 3′ to GBA, but a heterozygous region was clearly identified 144,388 nucleotides 5′ to the gene and beyon ...
Ch 6
... – Stop codons break genome into segments between consecutive Stop codons – The subsegments of these that start from the Start codon (ATG) are ORFs • ORFs in different frames may overlap ATG ...
... – Stop codons break genome into segments between consecutive Stop codons – The subsegments of these that start from the Start codon (ATG) are ORFs • ORFs in different frames may overlap ATG ...
Gene Prediction: Statistical Approaches
... – Stop codons break genome into segments between consecutive Stop codons – The subsegments of these that start from the Start codon (ATG) are ORFs • ORFs in different frames may overlap ATG ...
... – Stop codons break genome into segments between consecutive Stop codons – The subsegments of these that start from the Start codon (ATG) are ORFs • ORFs in different frames may overlap ATG ...
Complete comparative genomic analysis of two field isolates of
... variable region is located between hr1 and orf31 (bro-c) (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes ...
... variable region is located between hr1 and orf31 (bro-c) (v90/4, 15?0–27?1 kb; v90/2, 15?0–28?3 kb). In this 12?1 kb region (7?7 % of the genome), there are 261 of the 521 point mutations, accounting for 50 % of the total nucleotide changes. Of the 261 point mutations, 82 cause nonsynonymous changes ...
THE SYNTHESIS OF DNA AND NUCLEAR PROTEIN BY
... (d) Estimations oj Nucleolar V illume in Irradiated Gonocytes A number of the 20 fL sections used during the DNA estimations were subsequently counterstained with yellowish eosin in order to estimate the volume of the nucleoli in irradiated gonocytes. The nucleoli in 20 nuclei from each of the two i ...
... (d) Estimations oj Nucleolar V illume in Irradiated Gonocytes A number of the 20 fL sections used during the DNA estimations were subsequently counterstained with yellowish eosin in order to estimate the volume of the nucleoli in irradiated gonocytes. The nucleoli in 20 nuclei from each of the two i ...
GENE TRANSCRIPTION AND RNA MODIFICATION File
... of RNA transcription. Most of the promoter region is located just ahead of or upstream from the site where transcription of a gene actually begins. By convention, the bases in a promoter sequence are numbered in relation to the transcriptional start site (Figure 12.4). This site is the first base us ...
... of RNA transcription. Most of the promoter region is located just ahead of or upstream from the site where transcription of a gene actually begins. By convention, the bases in a promoter sequence are numbered in relation to the transcriptional start site (Figure 12.4). This site is the first base us ...
Nuclear DNA Content Estimates in Multicellular
... contradict this view and present a compelling case that an ancient divergence separates green plants into two major monophyletic lineages: the Chlorophyta and the Streptophyta (McCourt, 1995; Karol et al., 2001). The Chlorophyta contain the classical ‘green algae’, primarily the Chlorophyceae and Ul ...
... contradict this view and present a compelling case that an ancient divergence separates green plants into two major monophyletic lineages: the Chlorophyta and the Streptophyta (McCourt, 1995; Karol et al., 2001). The Chlorophyta contain the classical ‘green algae’, primarily the Chlorophyceae and Ul ...
microencapsula tion of dna within cross
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
mic.sgmjournals.org
... DNA. This was based on genomic DNA organization in Escherichia coli and Bacillus subtilis. However, several bacteria have been found to have more than one chromosome; in addition, an unconventional linear structure has also been reported in several bacteria (for reviews, see Casjens, 1998; Volff & A ...
... DNA. This was based on genomic DNA organization in Escherichia coli and Bacillus subtilis. However, several bacteria have been found to have more than one chromosome; in addition, an unconventional linear structure has also been reported in several bacteria (for reviews, see Casjens, 1998; Volff & A ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.