Transcription blockage by stable H-DNA analogs in
... stable under physiological pH and ionic conditions. Because of that, the reverse Hoogsteen triplex is a more likely candidate for biologically relevant roles (for detailed review of various types of triplexes and their properties see (5)). One way to form a triplex at a homopurinehomopyrimidine DNA ...
... stable under physiological pH and ionic conditions. Because of that, the reverse Hoogsteen triplex is a more likely candidate for biologically relevant roles (for detailed review of various types of triplexes and their properties see (5)). One way to form a triplex at a homopurinehomopyrimidine DNA ...
Construction of Catalase Double Knockout Escherichia coli Strain
... Table 1, parenthesis indicate names used in this report. Cultures were grown in 2xYT medium (1.6% tryptone, 1% yeast extract, 0.5% NaCl for liquid or with addition of 1.5% agar for plating) at appropriate temperatures with appropriate addition of antibiotics, as indicated below in the growth conditi ...
... Table 1, parenthesis indicate names used in this report. Cultures were grown in 2xYT medium (1.6% tryptone, 1% yeast extract, 0.5% NaCl for liquid or with addition of 1.5% agar for plating) at appropriate temperatures with appropriate addition of antibiotics, as indicated below in the growth conditi ...
Localization and structural analysis of the ribosomal RNA operons of
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
Charge Transport in DNA - Insights from
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
APOBEC3A deaminates transiently exposed single-strand DNA
... expression inhibited human L1.3 retrotransposition to ∼28% of control levels (Figure 1B), in agreement with previous reports (Chen et al., 2006; Muckenfuss et al., 2006; Bogerd et al., 2006b). In comparison to control levels, A3A also inhibited retrotransposition of TGF21 (to ∼20%), ZfL2-2 (to ∼17%) ...
... expression inhibited human L1.3 retrotransposition to ∼28% of control levels (Figure 1B), in agreement with previous reports (Chen et al., 2006; Muckenfuss et al., 2006; Bogerd et al., 2006b). In comparison to control levels, A3A also inhibited retrotransposition of TGF21 (to ∼20%), ZfL2-2 (to ∼17%) ...
Multiplex in-vitro Detection using SERS
... which has been shown to offer an improvement in detection limits of three orders of magnitude, in comparison to fluorescence, for the detection of dye-labelled DNA.25 However, the main advantage SERS has compared to fluorescence spectroscopy is the ability to detect multiple components simultaneousl ...
... which has been shown to offer an improvement in detection limits of three orders of magnitude, in comparison to fluorescence, for the detection of dye-labelled DNA.25 However, the main advantage SERS has compared to fluorescence spectroscopy is the ability to detect multiple components simultaneousl ...
A Highly Efficient Method for the Construction of a Plasmid
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
Click “Next”, again. Answer question.
... These four letters represent the nitrogenous bases connected by hydrogen bonds on opposite complementary DNA strands. A stands for nitrogenous base, adenine; T stands for the nitrogenous base, thymine; G stands for the nitrogenous base, guanine and C stands for the nitrogenous base, cytosine. These ...
... These four letters represent the nitrogenous bases connected by hydrogen bonds on opposite complementary DNA strands. A stands for nitrogenous base, adenine; T stands for the nitrogenous base, thymine; G stands for the nitrogenous base, guanine and C stands for the nitrogenous base, cytosine. These ...
Highly sensitive DNA sensor based on upconversion nanoparticles
... (Figure 1A). These nanoparticles could be easily dispersed in organic solvents such as hexane due to the presence of oleic acid as capping and stabilizing agent. The UCNPs were subsequently coated with a silica shell by a reverse microemulsion method. With this procedure it was possible to obtain mo ...
... (Figure 1A). These nanoparticles could be easily dispersed in organic solvents such as hexane due to the presence of oleic acid as capping and stabilizing agent. The UCNPs were subsequently coated with a silica shell by a reverse microemulsion method. With this procedure it was possible to obtain mo ...
Systematic and Applied Microbiology - digital
... analyzed, produced tyramine. However, in both cases the production of biogenic amines was only evaluated in a decarboxylase agar medium where, sometimes, false-positive reactions have been described [10]. The results of our study demonstrate that the E. faecium strains analyzed actually possess a ge ...
... analyzed, produced tyramine. However, in both cases the production of biogenic amines was only evaluated in a decarboxylase agar medium where, sometimes, false-positive reactions have been described [10]. The results of our study demonstrate that the E. faecium strains analyzed actually possess a ge ...
Plant Functional Genomics Plant Functional Genomics
... Large genomic DNA insert-containing libraries are essential for physical mapping, positional cloning, and genome sequencing of complex genomes. There are two principal large insert cloning systems that are constructed as yeast or bacterial artificial chromosomes (YACs and BACs, respectively). The YA ...
... Large genomic DNA insert-containing libraries are essential for physical mapping, positional cloning, and genome sequencing of complex genomes. There are two principal large insert cloning systems that are constructed as yeast or bacterial artificial chromosomes (YACs and BACs, respectively). The YA ...
Natural rules for Arabidopsis thaliana
... site was calculated. A series paired group t-tests were performed to inspect distribution difference of each type of nucleotide between up- and down-stream regions of 5’ and 3’ splicing sites. The exact number differences of A, G, C (cytosine), U nucleotides across each splicing site was also calcul ...
... site was calculated. A series paired group t-tests were performed to inspect distribution difference of each type of nucleotide between up- and down-stream regions of 5’ and 3’ splicing sites. The exact number differences of A, G, C (cytosine), U nucleotides across each splicing site was also calcul ...
29th International Conference on Animal Genetics ISAG2004/TOKYO
... Construction of a high resolution comparative gene map between human chromosome 14 and swine chromosomes using RH mapping. Hiroshi Yasue (National Institute of Agrobiological Sciences) ...
... Construction of a high resolution comparative gene map between human chromosome 14 and swine chromosomes using RH mapping. Hiroshi Yasue (National Institute of Agrobiological Sciences) ...
Heavy Chain Diversity Region Segments of the Channel Catfish
... the catfish, a teleost (bony) fish, is different from that known in sharks. The C gene, which encodes the predominant serum Ig and Ab of catfish (14, 15), exists as a single genomic copy (16, 17), a general conclusion that has been extended to other teleost fish (18 –21). In addition, it has been s ...
... the catfish, a teleost (bony) fish, is different from that known in sharks. The C gene, which encodes the predominant serum Ig and Ab of catfish (14, 15), exists as a single genomic copy (16, 17), a general conclusion that has been extended to other teleost fish (18 –21). In addition, it has been s ...
Isolation, Characterization, and Annotation: The Search for Novel
... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... of Hoechst here was in ~ 1.5 - 2 times less than in the case of HP1 or t-RNA. It means that Hoechst interacts with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was ...
... of Hoechst here was in ~ 1.5 - 2 times less than in the case of HP1 or t-RNA. It means that Hoechst interacts with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was ...
Identification of novel epigenetic biomarkers in colorectal cancer
... monoclonal population. However, cytogenetic studies have shown that the acquisition of new mutant alleles by the cells in the population in some cases may result in genetic heterogeneity within the population, leading to polyclonality [2]. The cancer stem cell hypothesis was first proposed 150 years ...
... monoclonal population. However, cytogenetic studies have shown that the acquisition of new mutant alleles by the cells in the population in some cases may result in genetic heterogeneity within the population, leading to polyclonality [2]. The cancer stem cell hypothesis was first proposed 150 years ...
How the probability of a false positive affects the value of DNA
... rape case. The error led the laboratory to issue a report that mistakenly stated that the defendant was a potential contributor of what the analysts took to be “seminal stains” on the victim’s clothing (18). The report also stated that the defendant’s profile was “included” in a mixed sample taken f ...
... rape case. The error led the laboratory to issue a report that mistakenly stated that the defendant was a potential contributor of what the analysts took to be “seminal stains” on the victim’s clothing (18). The report also stated that the defendant’s profile was “included” in a mixed sample taken f ...
Molecular Identification of Nematodes Manual
... to their specific “binding sites.” We use this particular form of PCR where two primers are used in the reaction. They were specifically designed from a DNA template sequence (previously obtained from the literature or personal DNA sequencing) to be complimentary to that specific sequence, they are ...
... to their specific “binding sites.” We use this particular form of PCR where two primers are used in the reaction. They were specifically designed from a DNA template sequence (previously obtained from the literature or personal DNA sequencing) to be complimentary to that specific sequence, they are ...
Chapter 28. Heterocycles and Nucleic Acids
... There are 61 different tRNAs, one for each of the 61 ...
... There are 61 different tRNAs, one for each of the 61 ...
Chapter 28. Heterocycles and Nucleic Acids
... There are 61 different tRNAs, one for each of the 61 ...
... There are 61 different tRNAs, one for each of the 61 ...
The value of a reported DNA match for proving that two samples
... defendant was a potential contributor of what the analysts took to be “seminal stains” on the victim’s clothing (18). The report also stated that the defendant’s profile was “included” in a mixed sample taken from vaginal swabs. After the sample switch came to light, the laboratory reassessed the ev ...
... defendant was a potential contributor of what the analysts took to be “seminal stains” on the victim’s clothing (18). The report also stated that the defendant’s profile was “included” in a mixed sample taken from vaginal swabs. After the sample switch came to light, the laboratory reassessed the ev ...
DIFFERENTIAL GENE RESPONSE TO MUTAGENS IN
... of certain compounds (the mutagens themselves, or their metabolic complexes around the genetic material) a situation might arise simulating differential mutability, when in fact it is selective establishment of autotrophic populations derived from the same proportion of mutant cells attaining variou ...
... of certain compounds (the mutagens themselves, or their metabolic complexes around the genetic material) a situation might arise simulating differential mutability, when in fact it is selective establishment of autotrophic populations derived from the same proportion of mutant cells attaining variou ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.