gene mapping, marker gene mapping, marker-assisted
... 1999d; Tan et al. 1999, in review). In more than 100 clones, microsatellite sequences were immediately at the cloning site making them useless. There were 403 pairs of primers for their performance in amplifying channel catfish genomic DNA. Of this total, 257 pairs successfully amplified genomic DNA ...
... 1999d; Tan et al. 1999, in review). In more than 100 clones, microsatellite sequences were immediately at the cloning site making them useless. There were 403 pairs of primers for their performance in amplifying channel catfish genomic DNA. Of this total, 257 pairs successfully amplified genomic DNA ...
Gene Mapping, Marker-Assisted Selection, Gene Cloning, Genetic
... 1999d; Tan et al. 1999, in review). In more than 100 clones, microsatellite sequences were immediately at the cloning site making them useless. There were 403 pairs of primers for their performance in amplifying channel catfish genomic DNA. Of this total, 257 pairs successfully amplified genomic DNA ...
... 1999d; Tan et al. 1999, in review). In more than 100 clones, microsatellite sequences were immediately at the cloning site making them useless. There were 403 pairs of primers for their performance in amplifying channel catfish genomic DNA. Of this total, 257 pairs successfully amplified genomic DNA ...
dissertationes biologicae universitatis tartuensis 118
... The Rep helicase was crystallized in complex with single-stranded DNA (ssDNA) and ADP (Korolev et al., 1997). The complex consisted of 2 helicase molecules on the same DNA, one in open conformation and the other in the closed one, where the 2B domain was rotated 130o. For PcrA the crystal structures ...
... The Rep helicase was crystallized in complex with single-stranded DNA (ssDNA) and ADP (Korolev et al., 1997). The complex consisted of 2 helicase molecules on the same DNA, one in open conformation and the other in the closed one, where the 2B domain was rotated 130o. For PcrA the crystal structures ...
Mutagenesis Point mutations Deletions Insertions Types of
... Cloning. Gibson Assembly eliminates the need to engineer restriction enzyme cut sites within DNA when assembling fragments together. DNA molecules are designed such that neighboring fragments contain a 20-40 bp overlapping sequence. If the DNA fragments originate from PCR products, the overlapping s ...
... Cloning. Gibson Assembly eliminates the need to engineer restriction enzyme cut sites within DNA when assembling fragments together. DNA molecules are designed such that neighboring fragments contain a 20-40 bp overlapping sequence. If the DNA fragments originate from PCR products, the overlapping s ...
double-strand break repair pathways in dna structure
... (17-19). At the intersecting B-Z junctions, two bases are extruded from the helix and might be susceptible potential sites for DNA modification (20). Many proteins have been found to preferentially bind to the Z-DNA structure, such as the ADAR1 protein, vaccinia virus E3L protein, DLM-1 protein and ...
... (17-19). At the intersecting B-Z junctions, two bases are extruded from the helix and might be susceptible potential sites for DNA modification (20). Many proteins have been found to preferentially bind to the Z-DNA structure, such as the ADAR1 protein, vaccinia virus E3L protein, DLM-1 protein and ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... Chromosomes in a human cell are made up of two complementary strands of DNA molecules—one strand is on one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used t ...
... Chromosomes in a human cell are made up of two complementary strands of DNA molecules—one strand is on one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used t ...
Biology and Chemistry Primers - WWU Computer Science Faculty
... The double stranded DNA (dsDNA) includes exon regions made up triplets of bases pairs that encode a specific amino acid. Exons are expressed. T G G A C G T A C G T A T C G T A C C T G C A T G C A T A G C A ...
... The double stranded DNA (dsDNA) includes exon regions made up triplets of bases pairs that encode a specific amino acid. Exons are expressed. T G G A C G T A C G T A T C G T A C C T G C A T G C A T A G C A ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
Into to Linux Part 1-4
... – refer to the two ends of the same DNA molecule – After sequencing one end, you can turn it around and sequence the other end. – Long segment of DNA in between the two ends (usually 200-500 bp), who’s sequence is unknown – Once the two paired end reads are mapped, the intermediate sequence can be i ...
... – refer to the two ends of the same DNA molecule – After sequencing one end, you can turn it around and sequence the other end. – Long segment of DNA in between the two ends (usually 200-500 bp), who’s sequence is unknown – Once the two paired end reads are mapped, the intermediate sequence can be i ...
Nucleotide sequence and taxonomic value of the
... 1987), very little is known about the immunochemistry of this organism. One problem is that C. pneumoniae is difficult to isolate in tissue culture. Immunochemical and genetic studies should lead to simple and non-culturedependent diagnostic methodologies for this important organism, as has been the ...
... 1987), very little is known about the immunochemistry of this organism. One problem is that C. pneumoniae is difficult to isolate in tissue culture. Immunochemical and genetic studies should lead to simple and non-culturedependent diagnostic methodologies for this important organism, as has been the ...
OsHUS1 Facilitates Accurate Meiotic Recombination in Rice
... mutation of OsHUS1, we performed immunofluorescent examination using antibodies against ZEP1 in Oshus1-1 PMCs. ZEP1 is the transverse filament protein of SC in rice and hence, a perfect tool to mark the course of synapsis [30]. In leptotenic and zygotenic Oshus1-1 PMCs, the ZEP1 patterns appeared as ...
... mutation of OsHUS1, we performed immunofluorescent examination using antibodies against ZEP1 in Oshus1-1 PMCs. ZEP1 is the transverse filament protein of SC in rice and hence, a perfect tool to mark the course of synapsis [30]. In leptotenic and zygotenic Oshus1-1 PMCs, the ZEP1 patterns appeared as ...
Biophysics 101 Genomics and Computational Biology
... In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities. Isolation and properties of Escherichia coli ATPase mutants with altered divalent metal specificity for ATP hydrolysis. Isolation of altered specificity mutants of the single-chain 434 repressor that re ...
... In vivo selection of basic region-leucine zipper proteins with altered DNA-binding specificities. Isolation and properties of Escherichia coli ATPase mutants with altered divalent metal specificity for ATP hydrolysis. Isolation of altered specificity mutants of the single-chain 434 repressor that re ...
J. Biol. Chem.
... treatment of advanced colorectal cancer and second line treatment for ovarian cancer (7–9). As with most chemotherapeutics, these CPT analogs are administered in combination with other agents (10 –13). Although the molecular mechanisms of single agents may be well understood, the combination of two ...
... treatment of advanced colorectal cancer and second line treatment for ovarian cancer (7–9). As with most chemotherapeutics, these CPT analogs are administered in combination with other agents (10 –13). Although the molecular mechanisms of single agents may be well understood, the combination of two ...
1051 Identification of the Extinct Hawaiian Eagle (Haliaeetus) by
... analysis. The cyt-b sequences of H. albicilla, H. leucocephalus, and H. leucogaster were from a different study and therefore arise from different individuals than the sequences obtained for these same species in this study. Nonetheless, we combined the gene regions into the full 704 bp of sequence ...
... analysis. The cyt-b sequences of H. albicilla, H. leucocephalus, and H. leucogaster were from a different study and therefore arise from different individuals than the sequences obtained for these same species in this study. Nonetheless, we combined the gene regions into the full 704 bp of sequence ...
pdf
... removal of up to 60 Tg of nitrogen annually, roughly an eighth of the global fixed nitrogen sink. Although denitrification has long been believed to be the major process responsible for fixed nitrogen loss from the oceans, recent studies show that anaerobic ammonium oxidation (anammox) is potentiall ...
... removal of up to 60 Tg of nitrogen annually, roughly an eighth of the global fixed nitrogen sink. Although denitrification has long been believed to be the major process responsible for fixed nitrogen loss from the oceans, recent studies show that anaerobic ammonium oxidation (anammox) is potentiall ...
Non-homologous end-joining factors of Saccharomyces cerevisiae
... processes leading to mutagenesis, tumorigenesis and cell death. Therefore, efficient repair of DSB is essential to maintain genome stability and cell viability. Two main mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), have evolved for the repair of DSB in eukaryotic ce ...
... processes leading to mutagenesis, tumorigenesis and cell death. Therefore, efficient repair of DSB is essential to maintain genome stability and cell viability. Two main mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), have evolved for the repair of DSB in eukaryotic ce ...
Mechanistic Comparison of High-Fidelity and Error
... to DNA polymerization, it is first necessary to define fidelity. DNA polymerase fidelity has been measured in different ways, including (a) comparison of kinetic constants for Watson-Crick and mismatched nucleotide incorporation reactions and (b) measurement of misincorporation frequency when compet ...
... to DNA polymerization, it is first necessary to define fidelity. DNA polymerase fidelity has been measured in different ways, including (a) comparison of kinetic constants for Watson-Crick and mismatched nucleotide incorporation reactions and (b) measurement of misincorporation frequency when compet ...
here
... aThe first and second values represent the total for molecular and histological analyses, respectively. bproxy for Symbiodinium density. cdid not assess due to having mix of different species. dexogenous control enot LOF samples. Jan. 2016 ...
... aThe first and second values represent the total for molecular and histological analyses, respectively. bproxy for Symbiodinium density. cdid not assess due to having mix of different species. dexogenous control enot LOF samples. Jan. 2016 ...
- Penn State University
... There are several genetic level points of control currently used by engineers to raise protein expression, one of which is codon optimization of the coding sequences used by microorganisms to produce proteins. Since there are 20 amino acids but 64 codons, there are instances were multiple codons are ...
... There are several genetic level points of control currently used by engineers to raise protein expression, one of which is codon optimization of the coding sequences used by microorganisms to produce proteins. Since there are 20 amino acids but 64 codons, there are instances were multiple codons are ...
The type 4 pilin of Moraxella nonliquefaciens exhibits
... residue of the mature protein (McKern et al., 1983; Taylor et al., 1987). The amino acid sequence of the amino-terminal end of the pili in M . nonliquefaciens NCTC 7784 was determined by Frnrholm & Sletten (1977). Comparison of the amino acid sequence of type 4 pilin from different species to the or ...
... residue of the mature protein (McKern et al., 1983; Taylor et al., 1987). The amino acid sequence of the amino-terminal end of the pili in M . nonliquefaciens NCTC 7784 was determined by Frnrholm & Sletten (1977). Comparison of the amino acid sequence of type 4 pilin from different species to the or ...
PierceEtAl2004BioBull - Region 11 Math And Science Teacher
... (Carr et al., 2002). A single, 800-bp PCR product was obtained, then cloned into the pPCR-ScriptAmp SK (+) plasmid (Stratagene) and sequenced (model CEQ 8000, Beckman-Coulter)using the CEQ DTCS Quick Start Kit (Beckman-Coulter)and T3 sequencing primer. The second independentanalysis of the ChileanBl ...
... (Carr et al., 2002). A single, 800-bp PCR product was obtained, then cloned into the pPCR-ScriptAmp SK (+) plasmid (Stratagene) and sequenced (model CEQ 8000, Beckman-Coulter)using the CEQ DTCS Quick Start Kit (Beckman-Coulter)and T3 sequencing primer. The second independentanalysis of the ChileanBl ...
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... they could find this particular molecule, it might reveal the chemical nature of the gene. Avery and his team extracted a mixture of various molecules from the heat-killed bacteria. They carefully treated this mixture with enzymes that destroyed proteins, lipids, carbohydrates, and some other molecul ...
... they could find this particular molecule, it might reveal the chemical nature of the gene. Avery and his team extracted a mixture of various molecules from the heat-killed bacteria. They carefully treated this mixture with enzymes that destroyed proteins, lipids, carbohydrates, and some other molecul ...
Comparison of the Detection Limits of the Culture and PCR Methods
... laborious, requiring prolonged incubation, selective enrichment, and reduction of the background flora, which should be followed by biochemical identification tests (12, 13). Most laboratories are unable to diagnose the anaerobic, microaerophilic, and fastidious bacteria responsible for human gastro ...
... laborious, requiring prolonged incubation, selective enrichment, and reduction of the background flora, which should be followed by biochemical identification tests (12, 13). Most laboratories are unable to diagnose the anaerobic, microaerophilic, and fastidious bacteria responsible for human gastro ...
Collaborative coupling between polymerase and helicase for
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
... holoenzyme destabilizes the first few base pairs of the fork thereby increasing the efficiency of helicase unwinding. The model implies that both enzymes are localized at the fork, but does not require a specific interaction between them. The model quantitatively reproduces homologous and heterologo ...
Frazier, Tamara: The Use of Computational Methods to Describe and Establish Utility of a DNA Sequence for Purposes of Patenting
... composition of matter if they are “made by man”—i.e. created in the lab or isolated from nature. In general, matter in its naturally occurring state cannot be patented, but isolated and purified “products of nature” are eligible for patent protection. Thus, it is now clear that “a DNA sequence itse ...
... composition of matter if they are “made by man”—i.e. created in the lab or isolated from nature. In general, matter in its naturally occurring state cannot be patented, but isolated and purified “products of nature” are eligible for patent protection. Thus, it is now clear that “a DNA sequence itse ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.