DNA breathing dynamics distinguish binding from nonbinding
... Received February 28, 2012; Revised July 13, 2012; Accepted July 17, 2012 ...
... Received February 28, 2012; Revised July 13, 2012; Accepted July 17, 2012 ...
Comparison of the Detection Limits of the Culture and PCR Methods
... laborious, requiring prolonged incubation, selective enrichment, and reduction of the background flora, which should be followed by biochemical identification tests (12, 13). Most laboratories are unable to diagnose the anaerobic, microaerophilic, and fastidious bacteria responsible for human gastro ...
... laborious, requiring prolonged incubation, selective enrichment, and reduction of the background flora, which should be followed by biochemical identification tests (12, 13). Most laboratories are unable to diagnose the anaerobic, microaerophilic, and fastidious bacteria responsible for human gastro ...
Complete Characterization of the 3 Mouse Hereditary Hemochromatosis HFE Gene and
... indicate the size of the identified mRNAs. Lanes in A: 1, HepG2; 2, HepG2 TPA treated; 3, kidney; 4, lung; 5, small intestine; 6, large intestine; 7, testis; 8, leukocytes; 9, pancreas and 10, spleen. Lanes in B and C: 1, lung; 2, small intestine; 3, large intestine; 4, liver; 5, spleen; 6, kidney; ...
... indicate the size of the identified mRNAs. Lanes in A: 1, HepG2; 2, HepG2 TPA treated; 3, kidney; 4, lung; 5, small intestine; 6, large intestine; 7, testis; 8, leukocytes; 9, pancreas and 10, spleen. Lanes in B and C: 1, lung; 2, small intestine; 3, large intestine; 4, liver; 5, spleen; 6, kidney; ...
International Journal of Antimicrobial Agents ksgA mutations confer
... the growth of a wide variety of microorganisms, with reported low toxicity against plants, humans and other animals [2,4,5]. However, as an aminoglycoside, some degree of nephrotoxicity and ototoxicity is expected. Several Gram-negative bacteria, including Pseudomonas spp. and Escherichia coli strai ...
... the growth of a wide variety of microorganisms, with reported low toxicity against plants, humans and other animals [2,4,5]. However, as an aminoglycoside, some degree of nephrotoxicity and ototoxicity is expected. Several Gram-negative bacteria, including Pseudomonas spp. and Escherichia coli strai ...
Wheat, Fusarium toxins and disease: the good, the bad and the ugly
... ¾THE BAD: Fusarium head blight (FHB) is the most serious disease in wheat around the world. Fusarium graminearum is the causal agent of FHB which lowers wheat yield, damages grain quality, and causes animal feed refusal and illness in humans by the mycotoxins it produces- deoxynivalenol (DON), nival ...
... ¾THE BAD: Fusarium head blight (FHB) is the most serious disease in wheat around the world. Fusarium graminearum is the causal agent of FHB which lowers wheat yield, damages grain quality, and causes animal feed refusal and illness in humans by the mycotoxins it produces- deoxynivalenol (DON), nival ...
document
... Brown midrib (BMR) corn varieties have a natural mutation that results in low lignin in their cell walls, leading to increased digestibility, which is a highly desirable trait for silage maize production. Based on the sequence information from the public database, we designed oligos to amplify the C ...
... Brown midrib (BMR) corn varieties have a natural mutation that results in low lignin in their cell walls, leading to increased digestibility, which is a highly desirable trait for silage maize production. Based on the sequence information from the public database, we designed oligos to amplify the C ...
Rates of Spontaneous Mutation
... since 1993, including one for a long-terminal-repeat retrotransposon. These retro-element rates are roughly an order of magnitude lower than the RNA-virus rates listed in Table 2. Because of the large mutational target sizes employed, the rate differences among these viruses may be real. (The rate d ...
... since 1993, including one for a long-terminal-repeat retrotransposon. These retro-element rates are roughly an order of magnitude lower than the RNA-virus rates listed in Table 2. Because of the large mutational target sizes employed, the rate differences among these viruses may be real. (The rate d ...
Genetic mapping of mutations using phenotypic pools and
... will open up because the amplification potential is now too weak to detect the positive allele (1/42 < 0.025). As still more individuals are added to the pool, the probability of obtaining additional positive alleles increases and the window size drops smoothly until the next multiple of 20 individu ...
... will open up because the amplification potential is now too weak to detect the positive allele (1/42 < 0.025). As still more individuals are added to the pool, the probability of obtaining additional positive alleles increases and the window size drops smoothly until the next multiple of 20 individu ...
Price, DK, Zhang, F, Ashley, CT and Warren, ST: The chicken FMR1 gene is highly conserved containing a CTT 5\' untranslated repeat and encodes an RNA-binding protein. Genomics 31:3-12 (1996).
... frequent inherited form of mental retardation. Fragile X syndrome segregates as an X-linked dominant disorder with reduced penetrance and has been mapped to the fragile X mental retardation (FMR1) gene located at human Xq27.3 (Warren and Ashley, 1995; Warren and Nelson, 1994). FMR1 contains a (CGG)n ...
... frequent inherited form of mental retardation. Fragile X syndrome segregates as an X-linked dominant disorder with reduced penetrance and has been mapped to the fragile X mental retardation (FMR1) gene located at human Xq27.3 (Warren and Ashley, 1995; Warren and Nelson, 1994). FMR1 contains a (CGG)n ...
Nucleic Acids: RNA and chemistry
... codes for a protein SO there needs to be a process for getting rid of the unwanted bases in the mRNA: that’s what splicing is! ...
... codes for a protein SO there needs to be a process for getting rid of the unwanted bases in the mRNA: that’s what splicing is! ...
Water SA
... SYBR Green is a non-specific dye which binds to any double-stranded DNA. Therefore, it will not only bind to PCR products but to non-specific products that have been amplified, as well as primer dimers. Due to this non-specificity, SYBR Green requires more time for optimisation than protocols based ...
... SYBR Green is a non-specific dye which binds to any double-stranded DNA. Therefore, it will not only bind to PCR products but to non-specific products that have been amplified, as well as primer dimers. Due to this non-specificity, SYBR Green requires more time for optimisation than protocols based ...
What is Sequence Alignment?
... Local Alignment: • Good for: searching for conserved patterns (domains or motifs) in DNA or protein sequences • Not good for: generating alignment of closely related sequences Global and local alignments are fundamentally similar and differ only in optimization strategy used in aligning similar resi ...
... Local Alignment: • Good for: searching for conserved patterns (domains or motifs) in DNA or protein sequences • Not good for: generating alignment of closely related sequences Global and local alignments are fundamentally similar and differ only in optimization strategy used in aligning similar resi ...
Evaluation of Forensic DNA Traces When Propositions of Interest
... traces, they commonly rely on only one number, expressing the rarity of the DNA profile in the population of interest. This is so because the focus is on propositions regarding the source of the recovered trace material, such as “the person of interest is the source of the crime stain.” In particula ...
... traces, they commonly rely on only one number, expressing the rarity of the DNA profile in the population of interest. This is so because the focus is on propositions regarding the source of the recovered trace material, such as “the person of interest is the source of the crime stain.” In particula ...
Use of an exogenous plasmid standard and quantitative PCR to
... seconds. Duplicate dilution series of purified E. faecalis DNA were used to construct the standard curve and negative duplicates (reactions without template) were run on each plate. Standard curves were based on reactions with starting quantities of 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg of E. ...
... seconds. Duplicate dilution series of purified E. faecalis DNA were used to construct the standard curve and negative duplicates (reactions without template) were run on each plate. Standard curves were based on reactions with starting quantities of 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg of E. ...
Reference Manual on Scientific Evidence Third Edition
... In sum, in little more than a decade, forensic DNA typing made the transition from a novel set of methods for identification to a relatively mature and wellstudied forensic technology. However, one should not lump all forms of DNA identification together. New techniques and applications continue to ...
... In sum, in little more than a decade, forensic DNA typing made the transition from a novel set of methods for identification to a relatively mature and wellstudied forensic technology. However, one should not lump all forms of DNA identification together. New techniques and applications continue to ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... cells as well as self-transfer between lacI33 bacteria. We found that TetR was frequently lost in episomal Lac⫹ revertant colonies that accumulate during selection with and without an imposed requirement for conjugal transfer. This loss was found many orders of magnitude more frequently than had bee ...
... cells as well as self-transfer between lacI33 bacteria. We found that TetR was frequently lost in episomal Lac⫹ revertant colonies that accumulate during selection with and without an imposed requirement for conjugal transfer. This loss was found many orders of magnitude more frequently than had bee ...
Alternative Splicing in Higher Plants
... Expressed sequence tags (ESTs) are often 500-700bp in length ESTs reflect a partial sequence of a gene (usually) given that genes are commonly >1000bp in length ESTs can be used to identify areas of the genomic sequence where there are genes – more intensive strategies are used to identify the full ...
... Expressed sequence tags (ESTs) are often 500-700bp in length ESTs reflect a partial sequence of a gene (usually) given that genes are commonly >1000bp in length ESTs can be used to identify areas of the genomic sequence where there are genes – more intensive strategies are used to identify the full ...
Extending the season of stone fruit by breeding late
... habit. To maintain continuity of supply to the supermarkets, growers need an early mid-season variety, with good quality and reliable cropping and spreading habit. A second gap is in early September, between ‘Victoria’ and ‘Marjorie’s Seedling’. A prerequisite for reliable cropping in plum, which fl ...
... habit. To maintain continuity of supply to the supermarkets, growers need an early mid-season variety, with good quality and reliable cropping and spreading habit. A second gap is in early September, between ‘Victoria’ and ‘Marjorie’s Seedling’. A prerequisite for reliable cropping in plum, which fl ...
Classification of colorectal cancer based on correlation of clinical
... appearance could have markedly different biological properties was highlighted by a second form of familial CRC: Lynch syndrome, known also as hereditary nonpolyposis colorectal cancer. In this condition it is evident that a high proportion of adenomas will, if left untreated, progress to CRC and do ...
... appearance could have markedly different biological properties was highlighted by a second form of familial CRC: Lynch syndrome, known also as hereditary nonpolyposis colorectal cancer. In this condition it is evident that a high proportion of adenomas will, if left untreated, progress to CRC and do ...
A mixed group ll/group III twintron in the Euglena
... assemble domains of proteins. This process is termed 'exon shuffling' (1, 2). An alternative view is that introns have been added to ancestral genes (3, 4, 5). Consequently, introns represent mobile or transposable elements invading protein coding regions during gene evolution. Insertion of transpos ...
... assemble domains of proteins. This process is termed 'exon shuffling' (1, 2). An alternative view is that introns have been added to ancestral genes (3, 4, 5). Consequently, introns represent mobile or transposable elements invading protein coding regions during gene evolution. Insertion of transpos ...
How Do We Understand Life?
... and to reproduce. By dividing into two cells, the mother cell passes on the blueprint for life, encoded in the DNA, to its two daughter cells, along with all of the other kinds of molecules that are necessary for these cells to live. It is apparent, even from looking at this relatively simple bacter ...
... and to reproduce. By dividing into two cells, the mother cell passes on the blueprint for life, encoded in the DNA, to its two daughter cells, along with all of the other kinds of molecules that are necessary for these cells to live. It is apparent, even from looking at this relatively simple bacter ...
transgenic animals - Worcester Polytechnic Institute
... cells perform these actions were not discovered until the 1950’s and 1960’s, and involve strand separation and the addition of complementary nucleotides using DNA polymerase. The ability to manipulate transgenes resulted from several fundamental advances to DNA biochemistry and recombinant DNA techn ...
... cells perform these actions were not discovered until the 1950’s and 1960’s, and involve strand separation and the addition of complementary nucleotides using DNA polymerase. The ability to manipulate transgenes resulted from several fundamental advances to DNA biochemistry and recombinant DNA techn ...
voltammetric methods for determination of some anticancer drugs
... antibiotic. It has a planar heterocyclic ring structure, the basic side groups critical for intercalation into DNA and the quinone prosthetic group, which together could confer chemical reactivity. The positively charged, nitrogen-containing side chains project out from the molecule, and stabilize t ...
... antibiotic. It has a planar heterocyclic ring structure, the basic side groups critical for intercalation into DNA and the quinone prosthetic group, which together could confer chemical reactivity. The positively charged, nitrogen-containing side chains project out from the molecule, and stabilize t ...
wsp Gene Sequences from the Wolbachia of Filarial Nematodes
... Japan, and USA; W. bancrofti from New Guinea and Sri Lanka) showed identical wsp gene sequences. This also agrees with previous ftsZ results and confirms that the Wolbachia–filaria association is stable and species specific. Are the trees in Fig. 1 to be regarded as organism trees or as gene trees? ...
... Japan, and USA; W. bancrofti from New Guinea and Sri Lanka) showed identical wsp gene sequences. This also agrees with previous ftsZ results and confirms that the Wolbachia–filaria association is stable and species specific. Are the trees in Fig. 1 to be regarded as organism trees or as gene trees? ...
RNA Blue - RNA/DNA/PROTEIN ISOLATION REAGENT - Top-Bio
... subsequent centrifugation at 7 500 x g for 5 minutes at 4 ‐ 25oC. Add at least 1 ml of 75% ethanol per 1 ml RNA Blue reagent used for the initial homogenization. If the RNA pellet accumulates on a side of the tube and has tendency to float, sediment the pellet at 12 000 x g. ...
... subsequent centrifugation at 7 500 x g for 5 minutes at 4 ‐ 25oC. Add at least 1 ml of 75% ethanol per 1 ml RNA Blue reagent used for the initial homogenization. If the RNA pellet accumulates on a side of the tube and has tendency to float, sediment the pellet at 12 000 x g. ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.