Sequence-specific RNA Photocleavage by Single
... before they could be broadly adapted as genomic or therapeutic tools. Since the first description of RNA interference (RNAi), there has been rapid progress towards its application as a therapeutic modality6. The mechanism of the RNAi is that the RNAi (the length of RNA must be longer than 21 bases), ...
... before they could be broadly adapted as genomic or therapeutic tools. Since the first description of RNA interference (RNAi), there has been rapid progress towards its application as a therapeutic modality6. The mechanism of the RNAi is that the RNAi (the length of RNA must be longer than 21 bases), ...
Full-Text PDF
... In all eukaryotic organisms, genome replication depends on activity of the primosome, a four-subunit complex of DNA primase and DNA polymerase α (Polα) [1]. The primosome initiates synthesis of both the leading and lagging strands by making chimeric RNA-DNA primers, which are required for the loadin ...
... In all eukaryotic organisms, genome replication depends on activity of the primosome, a four-subunit complex of DNA primase and DNA polymerase α (Polα) [1]. The primosome initiates synthesis of both the leading and lagging strands by making chimeric RNA-DNA primers, which are required for the loadin ...
Full Text
... Abstract: Sequence alignment is a bioinformatics application that determines the degree of similarity between nucleotide sequences which is assumed to have same ancestral relationships. This sequence alignment method reads query sequence from the user and makes an alignment against large and genomic ...
... Abstract: Sequence alignment is a bioinformatics application that determines the degree of similarity between nucleotide sequences which is assumed to have same ancestral relationships. This sequence alignment method reads query sequence from the user and makes an alignment against large and genomic ...
Analysis of Guanine Oxidation Products in Double
... DNA bases is accepted as one of the principal sources of genetic damage involved in genetic mutation, aging, and cell death [1–8]. Since the oxidation potentials for guanine, adenine, cytosine, and thymine are 1.29, 1.42, 1.6, 1.7 V, respectively [9], guanine is the most easily oxidized among the DN ...
... DNA bases is accepted as one of the principal sources of genetic damage involved in genetic mutation, aging, and cell death [1–8]. Since the oxidation potentials for guanine, adenine, cytosine, and thymine are 1.29, 1.42, 1.6, 1.7 V, respectively [9], guanine is the most easily oxidized among the DN ...
Estimation of grizzly bear population size for Swan Hills
... predict the probability of relative occupancy in the habitat areas surrounding the DNA site (pLR) based on environmental GIS predictor variables (Table 1). We assumed that the probability of detection (i.e. hair snag) for a bear that encountered a DNA hair snag site was constant across all sites sam ...
... predict the probability of relative occupancy in the habitat areas surrounding the DNA site (pLR) based on environmental GIS predictor variables (Table 1). We assumed that the probability of detection (i.e. hair snag) for a bear that encountered a DNA hair snag site was constant across all sites sam ...
The coiled-coil of the human Rad50 DNA repair protein contains
... he protein complex containing Rad50 and Mre11 (R兾M) plays a pivotal role in maintaining genome stability. Its vital importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes ( ...
... he protein complex containing Rad50 and Mre11 (R兾M) plays a pivotal role in maintaining genome stability. Its vital importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes ( ...
The genomic landscape of meiotic crossovers and gene
... identification at high accuracy and revealed an ∼80-kb transposition, which undergoes copy-number changes mediated by meiotic recombination. Non-crossover associated GCs were extremely rare most likely due to their short average length of ∼25–50 bp, which is significantly shorter than the length of ...
... identification at high accuracy and revealed an ∼80-kb transposition, which undergoes copy-number changes mediated by meiotic recombination. Non-crossover associated GCs were extremely rare most likely due to their short average length of ∼25–50 bp, which is significantly shorter than the length of ...
RecA-mediated strand exchange traverses
... However, there should be an efficient strand exchange between homologous DNA regions coding for functional proteins. In individual lines of organisms, their DNA is subject to stochastic mutations wherein base substitutions are the most frequent non-detrimental mutations in protein-coding genes. Thus ...
... However, there should be an efficient strand exchange between homologous DNA regions coding for functional proteins. In individual lines of organisms, their DNA is subject to stochastic mutations wherein base substitutions are the most frequent non-detrimental mutations in protein-coding genes. Thus ...
In the near future, EU thresholds for adventitious
... Impurity testing in seeds has traditionally been done using either single or sequential seed-pool tests. From an analytical seed sample (e.g. 3000 seeds) equal seed pools are made and analysed individually for the trait of interest. Using binomial statistics, the number of seed pools positive for th ...
... Impurity testing in seeds has traditionally been done using either single or sequential seed-pool tests. From an analytical seed sample (e.g. 3000 seeds) equal seed pools are made and analysed individually for the trait of interest. Using binomial statistics, the number of seed pools positive for th ...
Factorindependent transcription pausing caused by recognition of
... elongation is now recognized as an important step in regulation of gene expression. This regulation is accomplished through pausing of transcription. Several types of signals were shown to cause transcription elongation pausing: misalignment of the incoming nucleotide (Kireeva and Kashlev, 2009), se ...
... elongation is now recognized as an important step in regulation of gene expression. This regulation is accomplished through pausing of transcription. Several types of signals were shown to cause transcription elongation pausing: misalignment of the incoming nucleotide (Kireeva and Kashlev, 2009), se ...
Molecular events during translocation and proofreading extracted
... all inter-atomic or intra-molecular distances in a structure, thus a good measure of the particle size. For example, RB69 DNA polymerase in the open state is about 1 Å larger than that in the closed state judging by the difference in c1 for these two states (Figure 2C). ...
... all inter-atomic or intra-molecular distances in a structure, thus a good measure of the particle size. For example, RB69 DNA polymerase in the open state is about 1 Å larger than that in the closed state judging by the difference in c1 for these two states (Figure 2C). ...
Computer-Aided DNA Base Calling from Forward and Reverse
... an electropherogram that is a 4-component time series made of the samples of the emissions measured by the 4 optical sensors. In principle, the DNA sequence can be obtained from the electropherogram by associating each dominant peak with the corresponding base type and by preserving the order of the ...
... an electropherogram that is a 4-component time series made of the samples of the emissions measured by the 4 optical sensors. In principle, the DNA sequence can be obtained from the electropherogram by associating each dominant peak with the corresponding base type and by preserving the order of the ...
Analysis of expressed sequence tags from the Huperzia serrata leaf
... tetraketides (Morita et al. 2007, Wanibuchi et al. 2007). However, as of January 2009, there were only 10 nucleotide sequences from H . serrata available in the NCBI database. The limited information on the genetic contents of this plant triggered our efforts to construct a cDNA library from the H . ...
... tetraketides (Morita et al. 2007, Wanibuchi et al. 2007). However, as of January 2009, there were only 10 nucleotide sequences from H . serrata available in the NCBI database. The limited information on the genetic contents of this plant triggered our efforts to construct a cDNA library from the H . ...
xCh21 DNA W11
... Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
... Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
Construction of plant BAC libraries This document
... incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place the stained gel pieces aside. 13. Using a scalpel, coverglass, or razor blade, connect the incisions on each even-numbered gel piece as shown in FIGURE 2e. You should now have three unst ...
... incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place the stained gel pieces aside. 13. Using a scalpel, coverglass, or razor blade, connect the incisions on each even-numbered gel piece as shown in FIGURE 2e. You should now have three unst ...
Plant and Soil
... The seasonal fluctuations in the concentration of cultured denitrifying and N2 -fixing bacteria were followed in an ammonium fertilised and a control soil of a Norway spruce forest near Villingen/Black Forest from December 1994 to August 1998. The horizontal distribution of bacteria in three layers ...
... The seasonal fluctuations in the concentration of cultured denitrifying and N2 -fixing bacteria were followed in an ammonium fertilised and a control soil of a Norway spruce forest near Villingen/Black Forest from December 1994 to August 1998. The horizontal distribution of bacteria in three layers ...
Epigenetics and the environment: emerging
... phenotypic variations are indeed caused by differential epigenetic states, rather than resulting from genetic mutations. Therefore, as an alternative approach plants have been directly subjected to specific stresses, and long-term epigenetic effects were explored25. In fascinating recent work on ase ...
... phenotypic variations are indeed caused by differential epigenetic states, rather than resulting from genetic mutations. Therefore, as an alternative approach plants have been directly subjected to specific stresses, and long-term epigenetic effects were explored25. In fascinating recent work on ase ...
DNA Real Estate: The Myriad Genetics Case and the Implications of
... which have been estimated by the Human Genome Project to contain approximately twenty thousand to twenty-five thousand genes.5 Genes are encoded by means of deoxyribonucleic acid, commonly known as DNA;6 they are the source of hereditary traits and essentially serve as the “instructions to make mole ...
... which have been estimated by the Human Genome Project to contain approximately twenty thousand to twenty-five thousand genes.5 Genes are encoded by means of deoxyribonucleic acid, commonly known as DNA;6 they are the source of hereditary traits and essentially serve as the “instructions to make mole ...
RHD - Labex
... The results with Cp beyond 40 cycles are not to be considered as definitive, they must be checked ...
... The results with Cp beyond 40 cycles are not to be considered as definitive, they must be checked ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... To test whether the YAC clones harboring genes h49 and jl8 are mitotically stable in long term cultures, they were grown for 75 generations and examined by Southern blot analysis. No rearrangements were observed suggesting the YAC clones are propagated faithfully during mitosis. This observation was ...
... To test whether the YAC clones harboring genes h49 and jl8 are mitotically stable in long term cultures, they were grown for 75 generations and examined by Southern blot analysis. No rearrangements were observed suggesting the YAC clones are propagated faithfully during mitosis. This observation was ...
DNA phosphorothioation inStreptomyces lividans: mutational
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
Generalized Transduction by Phage P22 in Salmonella typhimurium. II. Mechanisms of Integration of Transducing DNA.
... bacteria. The molecular weight of most of the large integrated fragments is greater than 2 to 4 x IO8daltons but substantially less than 27 x lo6 daltons, the molecularweightof the transducingDNA injectedinto the bacteria.The integrated largedouble strandfragmentsof transducingDNA can be detected in ...
... bacteria. The molecular weight of most of the large integrated fragments is greater than 2 to 4 x IO8daltons but substantially less than 27 x lo6 daltons, the molecularweightof the transducingDNA injectedinto the bacteria.The integrated largedouble strandfragmentsof transducingDNA can be detected in ...
Competing Interests - Saudi Medical Journal
... samples, Taq polymerase (Promega, Madison, WI, USA) and dNTP-buffer mix. The PCR was performed following manufacturer instructions using Mastercycler epgradiant S eppendorf PCR system (Eppendorf, AG, Hambourg, Germany). After amplification, electrophoresis in 2.5% TBE agarose gel was undertaken, at ...
... samples, Taq polymerase (Promega, Madison, WI, USA) and dNTP-buffer mix. The PCR was performed following manufacturer instructions using Mastercycler epgradiant S eppendorf PCR system (Eppendorf, AG, Hambourg, Germany). After amplification, electrophoresis in 2.5% TBE agarose gel was undertaken, at ...
3D DNA Crystals and Nanotechnology
... crystallography began to reveal the structural and functional details of proteins, but nucleic acid crystal structures were largely limited to relatively simple dinucleoside phosphate structures [3], primarily owing to the expense of longer oligonucleotides of discrete sequences needed for crystalli ...
... crystallography began to reveal the structural and functional details of proteins, but nucleic acid crystal structures were largely limited to relatively simple dinucleoside phosphate structures [3], primarily owing to the expense of longer oligonucleotides of discrete sequences needed for crystalli ...
The New Genetics - Marine Biodiversity Center
... the experiment was repeated many times over. Trying to solve the mystery of the missing color blooms, Mendel imagined that the reproductive cells of his pea plants might contain discrete “factors,” each of which specified a particular trait, such as white flowers. Mendel reasoned that the ...
... the experiment was repeated many times over. Trying to solve the mystery of the missing color blooms, Mendel imagined that the reproductive cells of his pea plants might contain discrete “factors,” each of which specified a particular trait, such as white flowers. Mendel reasoned that the ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.