Lactobacillus kimchii sp. nov., a new species from kimchi
... spoilage bacteria. The antimicrobial activity of lactic acid bacteria is known to be due to organic acids, hydrogen peroxide, diacetyl and bacteriocins (Dahiya & Speck, 1968 ; Jay, 1982 ; Klaenhammer, 1988). Many lactic acid bacteria have been isolated from kimchi in Korea and some of them have been ...
... spoilage bacteria. The antimicrobial activity of lactic acid bacteria is known to be due to organic acids, hydrogen peroxide, diacetyl and bacteriocins (Dahiya & Speck, 1968 ; Jay, 1982 ; Klaenhammer, 1988). Many lactic acid bacteria have been isolated from kimchi in Korea and some of them have been ...
Base-pair neutral homozygotes can be discriminated by calibrated
... edu/cgi-bin/hgPcr) to minimize the likelihood that undesired products would co-amplify and interfere with the target sequence melting curves. Table 1 shows the variants, PCR primer sequences, concentrations and amplicon lengths. Some of the small amplicon PCR primers were designed to tightly flank th ...
... edu/cgi-bin/hgPcr) to minimize the likelihood that undesired products would co-amplify and interfere with the target sequence melting curves. Table 1 shows the variants, PCR primer sequences, concentrations and amplicon lengths. Some of the small amplicon PCR primers were designed to tightly flank th ...
(Chapter 9): Molecular Structure of DNA and RNA
... • To fulfill its role, the genetic material must meet several criteria – 1. Information: It must contain the information necessary to make an entire organism – 2. Transmission: It must be passed from parent to offspring – 3. Replication: It must be copied • In order to be passed from parent to offsp ...
... • To fulfill its role, the genetic material must meet several criteria – 1. Information: It must contain the information necessary to make an entire organism – 2. Transmission: It must be passed from parent to offspring – 3. Replication: It must be copied • In order to be passed from parent to offsp ...
Molecular Characterization of a Powdery Mildew Resistance Gene
... Wheat powdery mildew, caused by the obligate biotrophic parasitic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide and is promulgated by the heavy use of high-yielding semidwarf cultivars, irrigation, and nitrogen fertilizer (1). Resistance t ...
... Wheat powdery mildew, caused by the obligate biotrophic parasitic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide and is promulgated by the heavy use of high-yielding semidwarf cultivars, irrigation, and nitrogen fertilizer (1). Resistance t ...
Ehlers-Danlos syndrome type VIIA and VIIB result from splice
... 1991]. These mutations interfere with the normal splicing of exon 6, which encodes 24 amino acids that include the protease cleavage site at the amino-terminal end of the triple helix of the chain. Similar mutations in the COL1A2 gene that encodes the proa2(I) chains of type I procollagen result in ...
... 1991]. These mutations interfere with the normal splicing of exon 6, which encodes 24 amino acids that include the protease cleavage site at the amino-terminal end of the triple helix of the chain. Similar mutations in the COL1A2 gene that encodes the proa2(I) chains of type I procollagen result in ...
Jurisdiction, Privacy, and Ownership: DNA Technology and Field
... A mass fatality is an event resulting in more deaths than the local available resources can process.4 A mass fatality event can be manmade (armed conflict), natural disaster (tsunami), or accident that may be due to manmade or natural causes (plane crash). Mass fatalities occur under chaotic and unc ...
... A mass fatality is an event resulting in more deaths than the local available resources can process.4 A mass fatality event can be manmade (armed conflict), natural disaster (tsunami), or accident that may be due to manmade or natural causes (plane crash). Mass fatalities occur under chaotic and unc ...
HIV favors integration in active transcription units (TUs)
... proteomics to isolate and identify some of the functional proteins used by these potential insertion vectors could expand our understanding of the mechanisms used. A bioinformatics data base could then be used to see if there any DNA binding proteins, chromatin related proteins, DNase proteins, DNA ...
... proteomics to isolate and identify some of the functional proteins used by these potential insertion vectors could expand our understanding of the mechanisms used. A bioinformatics data base could then be used to see if there any DNA binding proteins, chromatin related proteins, DNase proteins, DNA ...
Preimplantation genetic diagnosis: State of the ART 2011
... aneuploidy screening, it is important to examine as many chromosomes as possible and up to 15 probes have been used (Baart et al. 2007). Since FISH is limited by the number of chromosomes that can be examined, many groups are replacing FISH with array-comparative genomic hybridisation (see below). M ...
... aneuploidy screening, it is important to examine as many chromosomes as possible and up to 15 probes have been used (Baart et al. 2007). Since FISH is limited by the number of chromosomes that can be examined, many groups are replacing FISH with array-comparative genomic hybridisation (see below). M ...
Positive and Negative Selection on Noncoding
... polymorphism data for 78 genes from 15 wild-caught M. m. castaneus individuals and divergence to a closely related species, Mus famulus. We find high levels of nucleotide diversity and moderate levels of selective constraint in upstream and downstream regions compared with nonsynonymous sites of pro ...
... polymorphism data for 78 genes from 15 wild-caught M. m. castaneus individuals and divergence to a closely related species, Mus famulus. We find high levels of nucleotide diversity and moderate levels of selective constraint in upstream and downstream regions compared with nonsynonymous sites of pro ...
Identification of Two Methionine Transfer RNA Genes in the Maize
... isoaccepting methionine elongator tRNA species may exist. The presence of these tRNA genes complements the findings of Chao et al. (9) who suggest by analysis of rRNA genes that the genome of maize mitochondria is more closely related to eubacteria than are the mitochondria of yeast or mammals. The ...
... isoaccepting methionine elongator tRNA species may exist. The presence of these tRNA genes complements the findings of Chao et al. (9) who suggest by analysis of rRNA genes that the genome of maize mitochondria is more closely related to eubacteria than are the mitochondria of yeast or mammals. The ...
Gel Electrophoresis - Integrated DNA Technologies
... smaller than about 100 bases in an agarose gel because the sieving properties of agarose are not fine enough. On the other end of the scale, molecules longer than about 25,000 bp but shorter than around 2,000,000 bp will all run at the same rate. This is called limiting mobility. Nucleic acid molecu ...
... smaller than about 100 bases in an agarose gel because the sieving properties of agarose are not fine enough. On the other end of the scale, molecules longer than about 25,000 bp but shorter than around 2,000,000 bp will all run at the same rate. This is called limiting mobility. Nucleic acid molecu ...
Unraveling DNA Repair in Human: Molecular Mechanisms and
... deoxyribose-phosphate chain, which excises the base residue in the free form and creates an AP (apurinic/apyrimidinic) site on sugarphosphate backbone of DNA. The class of enzymes called glycosylases carries out this release. Different DNA glycosylases remove different kinds of damage, and the speci ...
... deoxyribose-phosphate chain, which excises the base residue in the free form and creates an AP (apurinic/apyrimidinic) site on sugarphosphate backbone of DNA. The class of enzymes called glycosylases carries out this release. Different DNA glycosylases remove different kinds of damage, and the speci ...
Straightforward PCR optimization and highly flexible
... user may take maximum advantage of the new Mastercycler nexus X2 and its different 64-well block and 32-well block formats to accommodate different sample numbers. Therefore, the Mastercycler nexus X2 provides the user with the utmost flexibility while simultaneously delivering high quality results. ...
... user may take maximum advantage of the new Mastercycler nexus X2 and its different 64-well block and 32-well block formats to accommodate different sample numbers. Therefore, the Mastercycler nexus X2 provides the user with the utmost flexibility while simultaneously delivering high quality results. ...
CAIcal: A combined set of tools to assess codon usage adaptation
... Example of how to use the CAIcal server The CAIcal was used to annotate the genomic discontinuity in the E4 gene of human papilomavirus 1 (HPV1). Papillomaviruses (PVs) are a family of small dsDNA viruses that cause a variety of diseases including cervical cancer. The genome of PVs is modular with t ...
... Example of how to use the CAIcal server The CAIcal was used to annotate the genomic discontinuity in the E4 gene of human papilomavirus 1 (HPV1). Papillomaviruses (PVs) are a family of small dsDNA viruses that cause a variety of diseases including cervical cancer. The genome of PVs is modular with t ...
¯ - Mangel Labs
... for a cold hit. The RMP is the probability that a randomly selected individual would have the same genetic profile as the evidence sample, and is therefore a statistic which is wholly irrelevant and of no concern to the trier of fact in a case such as this where the match is a result of a data bank ...
... for a cold hit. The RMP is the probability that a randomly selected individual would have the same genetic profile as the evidence sample, and is therefore a statistic which is wholly irrelevant and of no concern to the trier of fact in a case such as this where the match is a result of a data bank ...
Identifying the Genetic Material
... The 35S-labeled and 32P-labeled phages were used to infect two separate batches of E. coli bacteria. Because radioactive elements release particles that can be detected with machines, they can be followed, or traced, in a biological process. Scientists could determine whether it was the DNA, the pro ...
... The 35S-labeled and 32P-labeled phages were used to infect two separate batches of E. coli bacteria. Because radioactive elements release particles that can be detected with machines, they can be followed, or traced, in a biological process. Scientists could determine whether it was the DNA, the pro ...
Manual: FullVelocity(TM) SYBR® Green QPCR - Gene X
... High-quality cDNA is essential for successful amplification with the FullVelocity SYBR Green QPCR master mix. Total and poly(A)+ RNA can be rapidly isolated and purified using Stratagene's Absolutely RNA® isolation kits, which are available for nano-, micro- and miniprep scale RNA purifications (Cat ...
... High-quality cDNA is essential for successful amplification with the FullVelocity SYBR Green QPCR master mix. Total and poly(A)+ RNA can be rapidly isolated and purified using Stratagene's Absolutely RNA® isolation kits, which are available for nano-, micro- and miniprep scale RNA purifications (Cat ...
The presence of two UvrB subunits in the UvrAB complex ensures
... signi®cant average volume. Since these unwrapped complexes are expected to have lost the ATP molecule, we decided to analyse these complexes further by determining the volumes of UvrB±DNA complexes that were isolated by washing in the absence of ATP. As expected, no wrapped complexes were observed a ...
... signi®cant average volume. Since these unwrapped complexes are expected to have lost the ATP molecule, we decided to analyse these complexes further by determining the volumes of UvrB±DNA complexes that were isolated by washing in the absence of ATP. As expected, no wrapped complexes were observed a ...
Definitions for annotating CDS sequences
... 3. Defining the Linker Sequences In the context of this analysis, “Linkers” refers to nucleotide sequences that flank the relevant CDS that will be evaluated on the nucleotide level but not at the amino acid level. From a molecular biology perspective, these are often thought of as “junction sequenc ...
... 3. Defining the Linker Sequences In the context of this analysis, “Linkers” refers to nucleotide sequences that flank the relevant CDS that will be evaluated on the nucleotide level but not at the amino acid level. From a molecular biology perspective, these are often thought of as “junction sequenc ...
Impact of Sample Type and DNA Isolation Procedure on
... isolation differed from all other communities (Fig. 2B). The average Bray-Curtis distance ...
... isolation differed from all other communities (Fig. 2B). The average Bray-Curtis distance ...
Nucleotide sequence analysis - Bioinformatics Unit
... nucleotide and protein databases. The emphasis of this tool is to find regions of sequence similarity. These can yield clues about the structure and function of this novel sequence, and about its evolutionary history and homology with other sequences in the database. •The fund ...
... nucleotide and protein databases. The emphasis of this tool is to find regions of sequence similarity. These can yield clues about the structure and function of this novel sequence, and about its evolutionary history and homology with other sequences in the database. •The fund ...
Biotechnology Explorer™ Ligation and Transformation - Bio-Rad
... designed to express a cDNA (complementary DNA) in a mammalian cell line, which is different again from one designed to add a tag to a protein for easy purification. The primary characteristics of any good vector include: ...
... designed to express a cDNA (complementary DNA) in a mammalian cell line, which is different again from one designed to add a tag to a protein for easy purification. The primary characteristics of any good vector include: ...
A novel assay for examining the molecular
... loaded on the DNA by RF-C (11–18, reviewed in 2,19,20). On the leading strand, PCNA is thought to be loaded once to establish a processive polymerase complex which can synthesize long stretches of DNA without further modification. On the lagging strand, each Okazaki fragment is initiated after synth ...
... loaded on the DNA by RF-C (11–18, reviewed in 2,19,20). On the leading strand, PCNA is thought to be loaded once to establish a processive polymerase complex which can synthesize long stretches of DNA without further modification. On the lagging strand, each Okazaki fragment is initiated after synth ...
Lecture Script for “Teaching Genetic Linkage and
... provide an opportunity for students to connect to the scenario.) “For example, many states (such as California) are experiencing extreme drought, leading to reduced crop yields and rising food prices in places that depend on California food crops. Not surprisingly, to address the impact of these fut ...
... provide an opportunity for students to connect to the scenario.) “For example, many states (such as California) are experiencing extreme drought, leading to reduced crop yields and rising food prices in places that depend on California food crops. Not surprisingly, to address the impact of these fut ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.