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Similarities and Differences between RNA and DNA Recognition by
Similarities and Differences between RNA and DNA Recognition by

... Let us first consider the problem confronting proteins interacting with either duplex DNA or the duplex portion of an RNA molecule. The threedimensional structure of double-stranded DNA is highly polymorphic (Kennard and Hunter 1989), but variations of two forms, A-form and Bform, are of relevance t ...
Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies
Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies

... identification of fetus-specific mRNA markers in maternal plasma from a chromosome involved in a chromosomal aneuploidy, all that remains would be to develop a technology for obtaining chromosome copy number information from such a marker (see later sections). One key advantage of plasma RNA markers ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR

... starch and casein, but could not produce hydrogen sulfide. It was ...
Unit VI – Structure and Function of DNA and RNA Unit VI: Structure
Unit VI – Structure and Function of DNA and RNA Unit VI: Structure

... blueprint for the synthesis of proteins, and proteins determine the traits of an organism. Proteins are made up of amino acids. Each amino acid is determined by a sequence of 3 base pairs of nitrogenous bases. (Ex. TACGATTTA) In this example there are 3 sets of 3 and may code for 3 different amino a ...
Chapter 7: Essentials of Molecular Biology Question: Which strand
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... is the consensus sequence for the -35 region of the promoter in bacteria? A. B. C. D. ...
Pourcel et al., Microbiology 2005
Pourcel et al., Microbiology 2005

... provides a new and robust identification tool. ...
draft dna profiling bill 2007
draft dna profiling bill 2007

... (xxxxi) “unknown deceased persons’ index” means an index of DNA profiles derived from forensic material of deceased persons whose identities are not known; (xxxxii) “validation” means process by which a procedure is evaluated to determine its efficacy and reliability for casework analysis and includ ...
J Mol Evol (2007) 65:541–554 - digital
J Mol Evol (2007) 65:541–554 - digital

... the possibility of amplifying more than one locus, and because individuals are likely to be heterozygous for these loci. We cloned PCR products resulting from the amplification of the complete second exon in all the species investigated here as well as long MHC fragments in which cloning was the onl ...
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... Hanahan and Weinberg [4] have summarized the capabilities a cell must acquire to become a cancer cell: 1) growth signal autonomy 2) evasion of apoptosis 3) insensitivity to antigrowth signals 4) sustained angiogenesis 5) limitless replicative potential and 6) capacity to invade tissue and grow at me ...
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Amplification of the BRCA2 Pathway Gene EMSY in Sporadic Breast
Amplification of the BRCA2 Pathway Gene EMSY in Sporadic Breast

... each other. The final ratios correspond to those of the test probes relative to the mean of the controls. Real-Time Quantitative PCR. Real-time PCR was carried out with an ABI Prism 7000 instrument (Applied Biosystems) under standard conditions using SYBR Green as detector. Briefly, PCR was done in ...
Part 1: Have Your DNA and Eat It Too!
Part 1: Have Your DNA and Eat It Too!

... 1. Create a 9 base DNA sequence and record it in your notebook. You will use this sequence to create a DNA strand that is nine bases long. You must use at least one of each base. You can choose any order with a few exceptions. You are NOT allowed to choose the following 3 bases in order: ATC, ATT, A ...
Nucleotide sequence of di hydrofolate reductase type VI
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... 17f 1 bp. Taking this into consideration,the hexamer -AGAAAT-, which was 18-bp downstream from the proposed - 35 sequence, could have formed the - 10 region. However, this sequence differed in three of the six base pairs found most commonly in E. coli - 10 sequences, -TATAAT-. If these regions did f ...
Curr. Microbiol. 42
Curr. Microbiol. 42

... group or for several groups by sets of degenerated primers. Three additional conserved blocks have recently been identified in the C-termini of Cry protoxins [29]. They may be exploited for extended PCR screening of cry genes, alone or together with the original five conserved blocks. For example, a ...
(base) sequence of the genome might reflect biological information
(base) sequence of the genome might reflect biological information

... When ATP1 was skipped 10, or 16 bases, the homologous area of ATP1 to the red-region of chromosome II was enlarged to 990 nt (Figure 2(c), 648 ~ 1,638), or 1,584 nt (Figure 2(d), 54 ~ 1,638), respectively. That is, the base sequence of the complete ATP1 gene had self-similarity to the gene-position ...
Introduction to Molecular Diagnostics
Introduction to Molecular Diagnostics

... Each complete human DNA helix contains a sequence of about three billion base pairs. The sequence of the bases is more than 99% identical in all people. The one percent of sequences that are variable is responsible for the differences that occur among humans. Our genes, which are the functional unit ...
Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić
Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić

... The aim of this work was to evaluate the applicability of SSCP analysis as a new tool for detection of phytoplasma molecular variability. Seventy phytoplasma isolates from grapevines, belonging to the 16SrXII-A ribosomal subgroup (stolbur/’Ca. P. solani’) were included in this study. Three different ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
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... for which it was desired to alter the surface of functional domains one ata time, with minimal misfolding o r structural alterations at a distance from the altered site. T h e first of these was called “alanine-scanning mutagenesis” and was used by CUNNINGHAMand WELLS(1989) to map the residues that ...
3-4 DNA F11 - De Anza College
3-4 DNA F11 - De Anza College

... And translated into a chain of amino acids in the cytoplasm. ...
Propeller-twisted Adenin.Thymine and Guanine.Cytosine Base Pairs
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... The coordinate system connected with a base pair was defined according to the Cambridge convention (Dickerson 1989). The Nl atom of pyrimidine base and N9 atom of purine base are symmetry-related by the x-axis, and the (/-axis goes through the C6 atom of pyrimidine and C8 atom of purine. The energy ...
Lesson Overview
Lesson Overview

... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: (1) The sugar in RNA is ribose instead of deoxyribose. (2) RNA is generally single-stranded and not double-s ...
Description of de novo copy number calls for cleft case
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... considers all possible copy number states including inherited deletions (e.g. `322'), MinimumDistance was developed specifically for detecting de novo copy number changes since the computational demands of the joint PennCNV HMM are substantial, and false positive identifications of de novo deletions ...
Lesson Overview
Lesson Overview

... Comparing RNA and DNA Each nucleotide in both DNA and RNA is made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. There are three important differences between RNA and DNA: (1) The sugar in RNA is ribose instead of deoxyribose. (2) RNA is generally single-stranded and not double-s ...
2 et 3 de couverture
2 et 3 de couverture

... DNeasy 96 Plant Kit (Qiagen). At CNR-IVV, samples were extracted according to Thomas et al. (1993) with some modifications. Fluorescent-labelled primers were used for PCR amplification. PCR products were electrophoresed on an ABI 3130 Genetic Analyzer (Applied Biosystems) and data analysis was perfo ...
localization of histone gene transcripts in newt lampbrush
localization of histone gene transcripts in newt lampbrush

... Ideal preparations have the bivalent chromosomes well separated from one another, all their lateral loops should be anchored down on the coverglass surface, and they should be reasonably free from nucleoli. These considerations determine the ideal oocyte size with which to work, as large as possible ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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