Supplementary Figure Legends (doc 34K)
... gene dosage. Custom high resolution array-CGH enables the accurate characterization of the deletion type. NF1 complete and large partial deletions were observed in 4.2% and 0.5% of NF1 patients in the French cohort, respectively. In absence of large deletion, molecular investigation of NF1 is achiev ...
... gene dosage. Custom high resolution array-CGH enables the accurate characterization of the deletion type. NF1 complete and large partial deletions were observed in 4.2% and 0.5% of NF1 patients in the French cohort, respectively. In absence of large deletion, molecular investigation of NF1 is achiev ...
Similarity Searches on Sequence Databases: BLAST
... compositions, e.g. many proteins contain patches known as low-complexity regions: such as segments that contain many prolines or glutamic acid residues. • If BLAST aligns two proline-rich domains, this alignment gets a very good E-value because of the high number of identical amino acids it contains ...
... compositions, e.g. many proteins contain patches known as low-complexity regions: such as segments that contain many prolines or glutamic acid residues. • If BLAST aligns two proline-rich domains, this alignment gets a very good E-value because of the high number of identical amino acids it contains ...
Development of Co-Dominant Amplified Polymorphic Sequence
... Resistance to M. grisea isolate PO6-6 in RIL29 is located on chromosome 11. Both Pi1 and Pi7(t) initially were mapped using a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived fr ...
... Resistance to M. grisea isolate PO6-6 in RIL29 is located on chromosome 11. Both Pi1 and Pi7(t) initially were mapped using a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived fr ...
Reconstruction of the original mycoflora in pelleted feed by PCR
... crossing points from the qPCR of feed samples were converted into log10 CFU g1 feed using a standard curve of A. flavus. Thus, the level of fungal contamination in feed A, B, C, and D is equivalent to 4.79 (standard deviation: r = 0.13), 5.69 (r = 0.03), 5.48 (r = 0.14), and 4.77 (r = 0.05) log10 C ...
... crossing points from the qPCR of feed samples were converted into log10 CFU g1 feed using a standard curve of A. flavus. Thus, the level of fungal contamination in feed A, B, C, and D is equivalent to 4.79 (standard deviation: r = 0.13), 5.69 (r = 0.03), 5.48 (r = 0.14), and 4.77 (r = 0.05) log10 C ...
Advances in molecular ecology: tracking trophic links through
... 2. Numerous invasive and non-invasive methods have been developed to characterize predator–prey interactions but two principal areas dominate ‘molecular’ research. These are reviewed under the headings of monoclonal antibodies and DNA-based techniques. 3. Early ‘molecular’ studies of predator–prey f ...
... 2. Numerous invasive and non-invasive methods have been developed to characterize predator–prey interactions but two principal areas dominate ‘molecular’ research. These are reviewed under the headings of monoclonal antibodies and DNA-based techniques. 3. Early ‘molecular’ studies of predator–prey f ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person out there, it was great to hear about DNA’s commitment a ...
... in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person out there, it was great to hear about DNA’s commitment a ...
Reverse Transcription (cDNA Synthesis) | NEB
... with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 50°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length. ...
... with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 50°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length. ...
Synthesis and Evaluation of Guanidino Phthalocyanines for G
... structures. This is perhaps to be expected since the 20 amino acid residues of proteins have a far greater range of chemical and physical properties than do the four DNA bases. Nevertheless, many RNA and single stranded DNA have well-defined tertiary structures. ...
... structures. This is perhaps to be expected since the 20 amino acid residues of proteins have a far greater range of chemical and physical properties than do the four DNA bases. Nevertheless, many RNA and single stranded DNA have well-defined tertiary structures. ...
Phylogenetic analysis of members of the genus Rickettsia using the
... and ompA. Based on phylogenetic data, the current classification of the genus Rickettsia is inappropriate, specifically its division into two groups, typhus and spotted fever. Integration of phenotypic, genotypic and phylogenetic data will contribute to the definition of a polyphasic taxonomy as has ...
... and ompA. Based on phylogenetic data, the current classification of the genus Rickettsia is inappropriate, specifically its division into two groups, typhus and spotted fever. Integration of phenotypic, genotypic and phylogenetic data will contribute to the definition of a polyphasic taxonomy as has ...
DNA sequence selectivity of guanine–N7 alkylation by nitrogen
... known sequence, the lengths of the labeled fragments produced after alkylation and subsequent alkaline piperidine treatment indicate the position of the original alkylation (18). Fragments differing in size by only one nucleotide can be resolved on high resolution DNA sequencing gels, and the intens ...
... known sequence, the lengths of the labeled fragments produced after alkylation and subsequent alkaline piperidine treatment indicate the position of the original alkylation (18). Fragments differing in size by only one nucleotide can be resolved on high resolution DNA sequencing gels, and the intens ...
Understanding Genetics
... The nature of genes and how they are arranged and expressed is described in Lectures Four through Nine. The search for what the gene really is made of quickly focused on DNA. Circumstantial evidence favored it: DNA was in the right place at the right times in the right amounts. But these were correl ...
... The nature of genes and how they are arranged and expressed is described in Lectures Four through Nine. The search for what the gene really is made of quickly focused on DNA. Circumstantial evidence favored it: DNA was in the right place at the right times in the right amounts. But these were correl ...
Structure solution of DNA-binding proteins and complexes with
... Zinc-finger fragments used as search models (PDB code 1f2i is shown as an example). The zinc-finger fragments were truncated stepwise during the target structure-solution procedure to investigate systematically the tradeoff between fragment completeness and accuracy of the binding motif for the solu ...
... Zinc-finger fragments used as search models (PDB code 1f2i is shown as an example). The zinc-finger fragments were truncated stepwise during the target structure-solution procedure to investigate systematically the tradeoff between fragment completeness and accuracy of the binding motif for the solu ...
Read the Nobel Lecture
... piece of DNA containing non-telomeric sequences. The idea was that if there was an enzyme that actively elongated telomeres, we might be able to detect it through its activity in association with telomere DNA. For this assay, we developed a substrate that was meant to mimic a telomere in the cell: a ...
... piece of DNA containing non-telomeric sequences. The idea was that if there was an enzyme that actively elongated telomeres, we might be able to detect it through its activity in association with telomere DNA. For this assay, we developed a substrate that was meant to mimic a telomere in the cell: a ...
PDF
... in twisting macromolecules. Vertical orientation of the field (Figure 2B) produces a torque-free tether, which might be useful in applications where stretching and twisting need to be uncoupled [20]. Using NdFeB magnets and the widely used 2.8 µm beads, one can easily produce forces up to about 20 p ...
... in twisting macromolecules. Vertical orientation of the field (Figure 2B) produces a torque-free tether, which might be useful in applications where stretching and twisting need to be uncoupled [20]. Using NdFeB magnets and the widely used 2.8 µm beads, one can easily produce forces up to about 20 p ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
Replication of Nuclear DNA
... synthesised strands are completed by the ligation of Okazaki fragments and repliconlength pieces by DNA ligase 1. What will now be especially interesting is the integration of these molecular events into higher levels of control, such as the specification of meristem identity. ...
... synthesised strands are completed by the ligation of Okazaki fragments and repliconlength pieces by DNA ligase 1. What will now be especially interesting is the integration of these molecular events into higher levels of control, such as the specification of meristem identity. ...
Interpretation Guidelines
... subject to stochastic effects, where two alleles at a heterozygous locus exhibit considerably different peak heights (i.e., peak height ratio generally <60%) or an allele fails to amplify to a detectable level (i.e., allelic dropout). ...
... subject to stochastic effects, where two alleles at a heterozygous locus exhibit considerably different peak heights (i.e., peak height ratio generally <60%) or an allele fails to amplify to a detectable level (i.e., allelic dropout). ...
DNA-based biosensor for the electrocatalytic determination of
... rent of NADH (de-los-Santos-Alvarez et al., 2006; Mano and Kuhn, 1999; Toh et al., 2003). This effect is not completely understood at the molecular level, although it is speculated that divalent cations can effectively counterbalance the negative charge of the catalyst favouring the approach of the ...
... rent of NADH (de-los-Santos-Alvarez et al., 2006; Mano and Kuhn, 1999; Toh et al., 2003). This effect is not completely understood at the molecular level, although it is speculated that divalent cations can effectively counterbalance the negative charge of the catalyst favouring the approach of the ...
The msh2 Gene of Schizosaccharomyces pombe Is
... and predisposition to a common form of colon cancer (hereditary nonpolyposis colon cancer) (46, 70). Inactivation of the murine MSH2 gene results in increased mutation rates, microsatellite instability, and cancer but has no effect on fertility (15). In contrast, MLH1-deficient mice and PMS2-deficie ...
... and predisposition to a common form of colon cancer (hereditary nonpolyposis colon cancer) (46, 70). Inactivation of the murine MSH2 gene results in increased mutation rates, microsatellite instability, and cancer but has no effect on fertility (15). In contrast, MLH1-deficient mice and PMS2-deficie ...
DNA Identification Science: An Introduction for Lawyers
... sentence variants called "alleles". Forensic identification, however, relies on relatively unique features that can distinguish between different people; conserved genes do not serve this purpose. Therefore, forensic scientists use non-coding DNA locations (or "loci"), to develop highly polymorphic ...
... sentence variants called "alleles". Forensic identification, however, relies on relatively unique features that can distinguish between different people; conserved genes do not serve this purpose. Therefore, forensic scientists use non-coding DNA locations (or "loci"), to develop highly polymorphic ...
Motifs that discriminate functions in genomic regions
... protein is its binding site, which can be characterized as a motif — either a specific string or a position-specific weight matrix, often a consensus of sequences at multiple binding sites. The binding sites for many regulatory proteins have been determined by sequencing DNA segments with a high aff ...
... protein is its binding site, which can be characterized as a motif — either a specific string or a position-specific weight matrix, often a consensus of sequences at multiple binding sites. The binding sites for many regulatory proteins have been determined by sequencing DNA segments with a high aff ...
File - Jaidean St Onge
... They have worked in the anthropological field for many years and have continued to keep researching. The journal is peer-reviewed and shows the research and methods that went into determining the results that they found. h. Relevance to biological anthropology: I found relevance to biological anthro ...
... They have worked in the anthropological field for many years and have continued to keep researching. The journal is peer-reviewed and shows the research and methods that went into determining the results that they found. h. Relevance to biological anthropology: I found relevance to biological anthro ...
Studies on Polynucleotides
... The two deoxyribonucleoside triphosphates used had equal specific activity. It can be seen that about 1.8 moles of dCMP were incorporated per mole of the template when only [3H]dCTP was present. When 1 In some experiments using the 1 strand of &30psu111 DNA (IL. C. Miller and J. H. van de Sande, unp ...
... The two deoxyribonucleoside triphosphates used had equal specific activity. It can be seen that about 1.8 moles of dCMP were incorporated per mole of the template when only [3H]dCTP was present. When 1 In some experiments using the 1 strand of &30psu111 DNA (IL. C. Miller and J. H. van de Sande, unp ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.