Phylogenomics of type II DNA topoisomerases
... the formation of a compensatory negative superturn into the topologically closed enzyme-free DNA. The positive superturn formed around gyrase is then converted into a negatively supercoiled one by the strand-transfer reaction of the enzyme, producing two negative superturns per cycle of topoisomeris ...
... the formation of a compensatory negative superturn into the topologically closed enzyme-free DNA. The positive superturn formed around gyrase is then converted into a negatively supercoiled one by the strand-transfer reaction of the enzyme, producing two negative superturns per cycle of topoisomeris ...
Male-killing i n two species of insect Wolbachia 1~ THE ROYAL
... A. bipunctata and A. encedon lines bearing male-killers were then tested for the presence of Wolbachia using Wolbachia-specific PCR. Initially, we tested the male-killing strains for Wolbachia using a primer pair (ftsZfl, ftsZrl) that permits amplification of the ftsZ gene of all Wolbachia, but not ...
... A. bipunctata and A. encedon lines bearing male-killers were then tested for the presence of Wolbachia using Wolbachia-specific PCR. Initially, we tested the male-killing strains for Wolbachia using a primer pair (ftsZfl, ftsZrl) that permits amplification of the ftsZ gene of all Wolbachia, but not ...
How Cations Can Assist DNase I in DNA Binding and Hydrolysis
... region that interacts with DNA (orange, bottom). The highlighted DNA binding region was defined by the residues losing their solvent accessibility when complexed with DNA, as described in the Materials and Methods section. doi:10.1371/journal.pcbi.1001000.g001 ...
... region that interacts with DNA (orange, bottom). The highlighted DNA binding region was defined by the residues losing their solvent accessibility when complexed with DNA, as described in the Materials and Methods section. doi:10.1371/journal.pcbi.1001000.g001 ...
20.4 RNA polymerase I has a bipartite promoter
... Ten subunits of the yeast RNA polymerase II have been located on the crystal structure, as shown in Figure 20.3 (1714). The catalytic site is formed by a cleft between the two large subunits (#1 and #2), which hold DNA downstream in "jaws". The structure is generally similar to that of bacterial RN ...
... Ten subunits of the yeast RNA polymerase II have been located on the crystal structure, as shown in Figure 20.3 (1714). The catalytic site is formed by a cleft between the two large subunits (#1 and #2), which hold DNA downstream in "jaws". The structure is generally similar to that of bacterial RN ...
pptx - Department of Computer Science and Engineering
... BMEG3102 Bioinformatics | Kevin Yip-cse-cuhk | Spring 2015 ...
... BMEG3102 Bioinformatics | Kevin Yip-cse-cuhk | Spring 2015 ...
TaqMan-Based Real-Time PCR for Genotyping Common
... frequencies of the HP1 and HP2 genotypes vary worldwide depending on the ethnic group, with the HP1 frequency varying from about 0.07 in parts of India to ⬎0.7 in parts of West Africa and South America (3 ). Because of its polymorphic nature, HP has been used as a genetic marker for identifying pare ...
... frequencies of the HP1 and HP2 genotypes vary worldwide depending on the ethnic group, with the HP1 frequency varying from about 0.07 in parts of India to ⬎0.7 in parts of West Africa and South America (3 ). Because of its polymorphic nature, HP has been used as a genetic marker for identifying pare ...
Receptions and Misconceptions of DNA Evidence in the New
... As the “customers” for forensic evidence,1 criminal justice systems have benefitted immeasurably from the development of DNA2 profiling technologies. Further refinements to the specificity and sensitivity of analytical techniques have widened the circumstances in which DNA can be of forensic use, in ...
... As the “customers” for forensic evidence,1 criminal justice systems have benefitted immeasurably from the development of DNA2 profiling technologies. Further refinements to the specificity and sensitivity of analytical techniques have widened the circumstances in which DNA can be of forensic use, in ...
Design-O-Saur - Beyond Benign
... Decode provided information to build a genetically unique dinosaur. Understand that this is a model that could be used to look at human genetics Understand gene sequences and the results of these sequences Materials: Clay – model magic works very well Student sheets Time needed: 1 x 45-60 ...
... Decode provided information to build a genetically unique dinosaur. Understand that this is a model that could be used to look at human genetics Understand gene sequences and the results of these sequences Materials: Clay – model magic works very well Student sheets Time needed: 1 x 45-60 ...
SURVEY AND SUMMARY New insights into Hoogsteen base pairs
... while, in contrast, G•C derivatives co-crystallized into the expected WC bps (6). The lack of experimental evidence for A•T/U WC bps raised considerable scepticism about the WC base-pairing scheme during 1960s and early 1970s as reviewed in (7). Computational studies on A•T base derivatives later sh ...
... while, in contrast, G•C derivatives co-crystallized into the expected WC bps (6). The lack of experimental evidence for A•T/U WC bps raised considerable scepticism about the WC base-pairing scheme during 1960s and early 1970s as reviewed in (7). Computational studies on A•T base derivatives later sh ...
safety in numbers? deciding when dna alone is enough to convict
... also never heard—and neither party volunteered or asked permission to state—that the chance that another prime-aged man living in the Bay Area in 1972 shared the matching profile was likely over fifty percent.6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of ...
... also never heard—and neither party volunteered or asked permission to state—that the chance that another prime-aged man living in the Bay Area in 1972 shared the matching profile was likely over fifty percent.6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of ...
Forensic Science Curriculum Guide - JSmithForensic
... this, law enforcement has had to continue to modify itself as criminal tendencies have become ever increasingly sophisticated. One would think that crime rates would eventually subside and level off, but the opposite is actually true. As new technologies are introduced to society, new crimes are dev ...
... this, law enforcement has had to continue to modify itself as criminal tendencies have become ever increasingly sophisticated. One would think that crime rates would eventually subside and level off, but the opposite is actually true. As new technologies are introduced to society, new crimes are dev ...
“EveryContact Leaves Its Trace”-Insight
... Dental Profiling:When dental records are unavailable and other methods of identification are not possible, the forensic dentist can often produce a "picture" of the general features of the individual. This process is known as post-mortem dental profiling. A dental profile will typically provide ...
... Dental Profiling:When dental records are unavailable and other methods of identification are not possible, the forensic dentist can often produce a "picture" of the general features of the individual. This process is known as post-mortem dental profiling. A dental profile will typically provide ...
Inference of homologous recombination in bacteria using whole
... donor contributes only a small contiguous segment of DNA whereas the recipient contributes the rest of the genome. For a given sample of bacteria, it is thus possible to define its clonal genealogy [7] irrespective of how frequently recombination happened, by tracing back in time the ancestry of th ...
... donor contributes only a small contiguous segment of DNA whereas the recipient contributes the rest of the genome. For a given sample of bacteria, it is thus possible to define its clonal genealogy [7] irrespective of how frequently recombination happened, by tracing back in time the ancestry of th ...
Introduction to Forensic Sciences - Beck-Shop
... a suspicious fluid or stain is saliva, semen or blood. Tests are available to determine if the evidence, such as blood, is of human origin. Once it is identified as human, then DNA (deoxyribonucleic acid) testing can be attempted. Forensic DNA testing is sometimes referred to as “DNA fingerprinting. ...
... a suspicious fluid or stain is saliva, semen or blood. Tests are available to determine if the evidence, such as blood, is of human origin. Once it is identified as human, then DNA (deoxyribonucleic acid) testing can be attempted. Forensic DNA testing is sometimes referred to as “DNA fingerprinting. ...
Safety in Numbers - Deciding When DNA Alone
... also never heard-and neither party volunteered or asked permission to state-that the chance that another prime-aged man living in the Bay Area in 1972 shared the matching profile was likely over fifty percent. 6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of ...
... also never heard-and neither party volunteered or asked permission to state-that the chance that another prime-aged man living in the Bay Area in 1972 shared the matching profile was likely over fifty percent. 6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of ...
Forensic Sciences
... specialists. The job of the forensic investigators will be to use science and technology to perform tests on the evidence collected. The results from these tests can then be used to support a theory dictating guilt or innocence. Forensic scientists use the same instruments and techniques used by sci ...
... specialists. The job of the forensic investigators will be to use science and technology to perform tests on the evidence collected. The results from these tests can then be used to support a theory dictating guilt or innocence. Forensic scientists use the same instruments and techniques used by sci ...
Genotype C of hepatitis B virus can be classified into at least two
... detected in Asia could be classified into at least two subgroups, which we named HBV/C1 and HBV/C2. Notably, HBV/C1 was found only in southeast Asia including Vietnam, Myanmar and Thailand, while HBV/C2 was found in Far East Asia including Japan, Korea and China. By means of phylogenetic analysis in ...
... detected in Asia could be classified into at least two subgroups, which we named HBV/C1 and HBV/C2. Notably, HBV/C1 was found only in southeast Asia including Vietnam, Myanmar and Thailand, while HBV/C2 was found in Far East Asia including Japan, Korea and China. By means of phylogenetic analysis in ...
Structure, stability and function of 5-chlorouracil
... Uracil analogs with halogen substitution at the 5-position represent an important class of compounds with regard to their mutagenic activity (1). Such analogs were first synthesized in the 1950s as potential anti-tumor agents (2,3). The 5-fluorouracil (FU) analog is a well-known anti-cancer drug for ...
... Uracil analogs with halogen substitution at the 5-position represent an important class of compounds with regard to their mutagenic activity (1). Such analogs were first synthesized in the 1950s as potential anti-tumor agents (2,3). The 5-fluorouracil (FU) analog is a well-known anti-cancer drug for ...
Reclassification of Koreibacter algae as a later heterotypic synonym
... anteiso-C17 : 0 as major components, but the quantitative ratios of the cellular fatty acids differed (Table S2). For reinvestigation of the base composition, genomic DNA from the two type strains was degraded to nucleosides by using P1 nuclease and bovine intestinal mucosa alkaline phosphatase, as ...
... anteiso-C17 : 0 as major components, but the quantitative ratios of the cellular fatty acids differed (Table S2). For reinvestigation of the base composition, genomic DNA from the two type strains was degraded to nucleosides by using P1 nuclease and bovine intestinal mucosa alkaline phosphatase, as ...
Resolving the Discrepancies Among Nucleic Acid Conformational
... approaches, designed to meet guidelines set by the nucleic acid community, permit rigorous comparative analyses of different three-dimensional structures, as well as allow for reconstruction of chain molecules at the base-pair level. The different computer programs, however, yield inconsistent descr ...
... approaches, designed to meet guidelines set by the nucleic acid community, permit rigorous comparative analyses of different three-dimensional structures, as well as allow for reconstruction of chain molecules at the base-pair level. The different computer programs, however, yield inconsistent descr ...
Computational Definition of
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
Genotyping Errors
... Genotyping is the process of determining the alleles inherited by an individual at one or more loci. ...
... Genotyping is the process of determining the alleles inherited by an individual at one or more loci. ...
Characterisation of Aeromonas species isolated from diseased fish
... 18 strains of Aeromonas were isolated from healthy or diseased fish and water samples collected from several areas of the Vidharbha region of Maharashtra state, India, for identification and characterization. Aeromonas isolates were characterized by Enterobacterial Repetitive Intergenic Consensus (E ...
... 18 strains of Aeromonas were isolated from healthy or diseased fish and water samples collected from several areas of the Vidharbha region of Maharashtra state, India, for identification and characterization. Aeromonas isolates were characterized by Enterobacterial Repetitive Intergenic Consensus (E ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.