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Genetic Engineering Genetic Engineering aka .. Genetic Modification Make changes in DNA code by Insert or delete specific genes Use modern molecular biology techniques. 1) Recombinant DNA Aka .. Gene Splicing made by combining DNA from > 1 source (often from very different species) 2) Gel electrophoresis An electric current smaller fragments faster larger ones – slower DNA fragments move to positive end + - A B DNA on murder weapon 3)Transgenic organisms carries a foreign gene, deliberately inserted into its genome. transgenic animal Mice--- so immune sys more like humans- can study ‘em Livestock --- leaner meat transgenic plant Soybeans/corn/crops --- to produce nat insecticiside so don’t have to be sprayed Also: Improved Nutritional Quality Insect Resistance Disease Resistance Drought Tolerance SMART 4) Cloning Clone --- genetically identical copy replacing the nucleus of an unfertilized ovum with the nucleus of a body cell from the organism. first adult mammal cloned was Dolly in 1997 SMART Come back!!!! 5) DNA Fingerprinting Compare genetic similarities/differences b/w individuals. often used to ID source of blood / tissue found at crime scenes. Polymerase Chain Reaction (PCR) 6) Gene Therapy the insertion of normal or genetically altered genes into cells usually to replace defective genes (cancer & genetic disorders) Eye cancer SMART My lab experience……. SMART Come back!!!!