Detection of GM Papaya Event 55-1 in Fresh
... Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene ...
... Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene ...
Sex-based individual variation of snake venom proteome among
... seven male snakes (B) was applied to 3–10 IPG strips (24 cm) followed by electrophoresis on 12% SDS-polyacrylamide gels. Gels were stained with silver. Circled outlines indicate gel regions that were chosen for comparison between the venoms. Arrows indicate spots seen only in female (A) or male (B) ...
... seven male snakes (B) was applied to 3–10 IPG strips (24 cm) followed by electrophoresis on 12% SDS-polyacrylamide gels. Gels were stained with silver. Circled outlines indicate gel regions that were chosen for comparison between the venoms. Arrows indicate spots seen only in female (A) or male (B) ...
The effecT of chlorinaTion of nucleoTide bases on The
... Fig. 4. The structure of the pairs of a conformers 42 of 5'-thymidylic acid (a) and 5-chloro-2'-deoxyuridine monophosphate (b) molecules where the cause of conformational parameters changes is turning of the phosphate group due to the formation and / or rupture of C7-H72···Oр аnd Op1-Hp1···Cl bonds ...
... Fig. 4. The structure of the pairs of a conformers 42 of 5'-thymidylic acid (a) and 5-chloro-2'-deoxyuridine monophosphate (b) molecules where the cause of conformational parameters changes is turning of the phosphate group due to the formation and / or rupture of C7-H72···Oр аnd Op1-Hp1···Cl bonds ...
Quantitative Analysis of the Kinetics of End
... of the few methods available for RecA, which provides a real-time measure of DNA binding, yet requires no arti®cial alterations in RecA or DNA structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that obser ...
... of the few methods available for RecA, which provides a real-time measure of DNA binding, yet requires no arti®cial alterations in RecA or DNA structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that obser ...
Purification and Some: Characteristics of a Monomeric Racemase
... NaCl connected to a fast protein liquid chromatography (FPLC) system. El&on was carried out with the standard buffer containing 0.2 M NaCl at a flow rate of 0.5 ml/min. After the active fractions were pooled, concentrated and dialyzed against the standard buffer, the solution was frozen and stored a ...
... NaCl connected to a fast protein liquid chromatography (FPLC) system. El&on was carried out with the standard buffer containing 0.2 M NaCl at a flow rate of 0.5 ml/min. After the active fractions were pooled, concentrated and dialyzed against the standard buffer, the solution was frozen and stored a ...
background of the invention
... With reference to FIG. 2 for a schematic view showing the layout of a field-effect transistor chip of a field-effect transistor type biosensor in accordance with an embodiment of the present invention, a field-effect transistor chip is fabrication by an n-well complementary metal oxide semiconductor ...
... With reference to FIG. 2 for a schematic view showing the layout of a field-effect transistor chip of a field-effect transistor type biosensor in accordance with an embodiment of the present invention, a field-effect transistor chip is fabrication by an n-well complementary metal oxide semiconductor ...
Identification of a Chloroplast-encoded 9-kDa
... gas-phase sequenator Model 479A usingthe program provided by the company. Phenylthiohydantoins were identified by reverse-phase high-pressure liquid chromatographyas described by Svendsen et al. (26). C-terminal amino acid analysis (27) was carried out by carboxypeptidase Y (649 ng) digestion of the ...
... gas-phase sequenator Model 479A usingthe program provided by the company. Phenylthiohydantoins were identified by reverse-phase high-pressure liquid chromatographyas described by Svendsen et al. (26). C-terminal amino acid analysis (27) was carried out by carboxypeptidase Y (649 ng) digestion of the ...
TRIzol Reagent
... Chomczynski, P. and Mackey, K. (1995) Anal. Biochem. 225, 163-164). The quantity and quality of RNA is the same with both reagents. The amount of BCP used for phase separation equals 10% of the TRIzol volume. Using lower centrifugation speeds: Centrifugation speeds as low as 5000 -6000 x g have been ...
... Chomczynski, P. and Mackey, K. (1995) Anal. Biochem. 225, 163-164). The quantity and quality of RNA is the same with both reagents. The amount of BCP used for phase separation equals 10% of the TRIzol volume. Using lower centrifugation speeds: Centrifugation speeds as low as 5000 -6000 x g have been ...
Comparing the Prevalence of Verotoxin
... viewing of the DNA bands. Next, I poured the agarose mixture into a casting tray and allowed it to solidify, which took about 20 minutes. I placed the gel into the electrophoresis chamber, and added buffer to the chamber until the gel was completely submerged. Loading samples into gel wells. The fir ...
... viewing of the DNA bands. Next, I poured the agarose mixture into a casting tray and allowed it to solidify, which took about 20 minutes. I placed the gel into the electrophoresis chamber, and added buffer to the chamber until the gel was completely submerged. Loading samples into gel wells. The fir ...
Polymerase Chain Reaction In Ophthalmology
... copies of a particular DNA sequence. So, broadly PCR can be described as “molecular photocopier” or more simply it can be termed as “DNA replication in a test tube”. Polymerase chain reaction was invented by Kary Mullis of USA1 in 1983 for which he was awarded the Nobel Prize in chemistry in 1993. H ...
... copies of a particular DNA sequence. So, broadly PCR can be described as “molecular photocopier” or more simply it can be termed as “DNA replication in a test tube”. Polymerase chain reaction was invented by Kary Mullis of USA1 in 1983 for which he was awarded the Nobel Prize in chemistry in 1993. H ...
Rabbit Reticulocyte Lysate Technical Manual
... isoprenylation and some phosphorylation activity that will vary from lot to lot (2). Processing events such as signal peptide cleavage and core glycosylation can be examined by adding Canine Pancreatic Microsomal Membranes (Cat.# Y4041) to a standard translation reaction (3). 1.B. Rabbit Reticulocy ...
... isoprenylation and some phosphorylation activity that will vary from lot to lot (2). Processing events such as signal peptide cleavage and core glycosylation can be examined by adding Canine Pancreatic Microsomal Membranes (Cat.# Y4041) to a standard translation reaction (3). 1.B. Rabbit Reticulocy ...
CLINICAL CHEMISTRY CHAPTER 5
... – In the last chapter, we discussed a variety of analytical techniques – In this chapter we’ll add some new techniques … They all involve the use of antibody – antigen reactions – Antibody – Antigen reactions have the advantage of being very specific ...
... – In the last chapter, we discussed a variety of analytical techniques – In this chapter we’ll add some new techniques … They all involve the use of antibody – antigen reactions – Antibody – Antigen reactions have the advantage of being very specific ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.