The demonstration of nickel in the urease of Helicobacter pylori by
... with 100 /~1 of 100 mM urea containing 0.2% (w/v) phenol red. After 10 rain the absorbance was read at 540 nm. ...
... with 100 /~1 of 100 mM urea containing 0.2% (w/v) phenol red. After 10 rain the absorbance was read at 540 nm. ...
TAP Express® Kit
... you will be able to transform any gene-of-interest into a transcriptionally active PCR (TAP) fragment that is ready for direct introduction into cells or animals. Because of its simplicity and speed, the TAP Express® technology is a powerful tool for functional genomics and basic research. Besides o ...
... you will be able to transform any gene-of-interest into a transcriptionally active PCR (TAP) fragment that is ready for direct introduction into cells or animals. Because of its simplicity and speed, the TAP Express® technology is a powerful tool for functional genomics and basic research. Besides o ...
Microplate-Based Pathlength Correction Method for Photometric
... pathlength of each well must be known before the BeerLambert Law can be applied to microplate measurements. If the microplate assay includes standards with known concentrations, pathlength correction is not needed. ...
... pathlength of each well must be known before the BeerLambert Law can be applied to microplate measurements. If the microplate assay includes standards with known concentrations, pathlength correction is not needed. ...
DNA sentences How are proteins coded for by DNA?
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a doublestranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribon ...
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a doublestranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribon ...
DNA sentences - seed2stem.org
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a double-stranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribo ...
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a double-stranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribo ...
Application Note 129 – Eppendorf MixMate
... As can be clearly seen from Figure 3, there is no crosscontamination or false-positive PCR signal in the negative controls (preparations without template DNA) as a result of the plate being mixed without being sealed, despite the relatively large PCR volume of 50 µl. Experiments performed to determi ...
... As can be clearly seen from Figure 3, there is no crosscontamination or false-positive PCR signal in the negative controls (preparations without template DNA) as a result of the plate being mixed without being sealed, despite the relatively large PCR volume of 50 µl. Experiments performed to determi ...
Nucleosides, Nucleotides,Nucleic Acids
... Watson and Crick proposed a double-stranded structure for DNA in which a purine or pyrimidine base in one chain is hydrogen bonded to its complement in the other. •Gives proper Chargaff ratios (A=T and G=C) •Because each pair contains one purine and one pyrimidine, the A---T and G---C distances betw ...
... Watson and Crick proposed a double-stranded structure for DNA in which a purine or pyrimidine base in one chain is hydrogen bonded to its complement in the other. •Gives proper Chargaff ratios (A=T and G=C) •Because each pair contains one purine and one pyrimidine, the A---T and G---C distances betw ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... torque and DNA supercoiling [38]. Optical tweezers, on the other hand, cover a very useful intermediate force range from a tenth of a piconewton to a nanonewton [126]. They are characterized by an extremely high spatiotemporal resolution, providing Ångström position resolution and millisecond time ...
... torque and DNA supercoiling [38]. Optical tweezers, on the other hand, cover a very useful intermediate force range from a tenth of a piconewton to a nanonewton [126]. They are characterized by an extremely high spatiotemporal resolution, providing Ångström position resolution and millisecond time ...
Properties of a newly characterized protein of the bovine - K-REx
... covalently bound in an amide linkage to the transacetylase subunit through the e-amino group of a lysine. The remaining approximately 300 amino acids contain the capacity to bind subunits and to catalyze the ...
... covalently bound in an amide linkage to the transacetylase subunit through the e-amino group of a lysine. The remaining approximately 300 amino acids contain the capacity to bind subunits and to catalyze the ...
Table of Contents - Appanna Lab
... The environment is very important to the health and safety of its inhabitants. This does not deal strictly with humans but also any wildlife and ecological habitats. Since the turn of the 19th and 20th centuries, we have become more reliant on industrialization1. Many of the luxuries in which we enj ...
... The environment is very important to the health and safety of its inhabitants. This does not deal strictly with humans but also any wildlife and ecological habitats. Since the turn of the 19th and 20th centuries, we have become more reliant on industrialization1. Many of the luxuries in which we enj ...
structure and mechanism of dna polymerases
... idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates for nucleotide insertion in the range of 10–3–10–6/base replicated and the diff ...
... idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates for nucleotide insertion in the range of 10–3–10–6/base replicated and the diff ...
Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
The Polymerase Chain Reaction
... – Single-stranded binding proteins prevent DNA from reannealing – Primase adds RNA nucleotides in the 5'3' direction – DNA polymerase III adds nucleotides to the ends of the RNA primers – DNA polymerase I corrects mistakes and removes RNA nucleotides and replaces them with DNA nucleotides – Ligase l ...
... – Single-stranded binding proteins prevent DNA from reannealing – Primase adds RNA nucleotides in the 5'3' direction – DNA polymerase III adds nucleotides to the ends of the RNA primers – DNA polymerase I corrects mistakes and removes RNA nucleotides and replaces them with DNA nucleotides – Ligase l ...
e Study of RNA Polymerase Pausing by Optical Traps
... downstream DNA. In the Class II ops pause (right), the RNAP moves backward along the RNADNA hybrid so that the RNA transcript is excluded from the NTP entry channel of the polymerase. ...
... downstream DNA. In the Class II ops pause (right), the RNAP moves backward along the RNADNA hybrid so that the RNA transcript is excluded from the NTP entry channel of the polymerase. ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.