Vitis 37 (3), 119
... Amplification was performed in a Perkin Elmer DNA Thermal Cycler 480. After 5 min at 94 °C, 34 cycles of PCR were performed, (l min at 94 °C, l min at 44 °C, 2 min at 72 °C) followed by 10 min at 72 °C for extension. G e l e l e c t r o p h o r e s i s : Aliquots of the RAPD products were analysed i ...
... Amplification was performed in a Perkin Elmer DNA Thermal Cycler 480. After 5 min at 94 °C, 34 cycles of PCR were performed, (l min at 94 °C, l min at 44 °C, 2 min at 72 °C) followed by 10 min at 72 °C for extension. G e l e l e c t r o p h o r e s i s : Aliquots of the RAPD products were analysed i ...
5 DNA Replication
... must start a new at the replication fork and proceed in the direction opposite that of the movement of the fork until it runs into the previously replicated segment of DNA. This process is repeated again and again, so synthesis of this strand is in short, discontinuous bursts. The newly made strand ...
... must start a new at the replication fork and proceed in the direction opposite that of the movement of the fork until it runs into the previously replicated segment of DNA. This process is repeated again and again, so synthesis of this strand is in short, discontinuous bursts. The newly made strand ...
Immobilization_Mecha..
... was highly dependent on the phosphorylation state of the probe DNA. For the DADA film, DNA immobilization and hybridization was less dependent on phosphorylation state, and maximum hybridization (as measured by PicoGreen fluorescence intensity) of phosphorylated DNA was reduced. However, these results ...
... was highly dependent on the phosphorylation state of the probe DNA. For the DADA film, DNA immobilization and hybridization was less dependent on phosphorylation state, and maximum hybridization (as measured by PicoGreen fluorescence intensity) of phosphorylated DNA was reduced. However, these results ...
A structural determinant in the uracil DNA glycosylase superfamily
... ng of E. coli genomic DNA, 200 nM forward primer UDGNdeI and reverse primer UDG-HindIII, 1 x Taq PCR buffer (New England Biolabs), 200 M each dNTP and 5 units of Taq DNA polymerase (New England Biolabs). The PCR procedure included a predenaturation step at 94◦ C for 3 min, 30 cycles of three-step a ...
... ng of E. coli genomic DNA, 200 nM forward primer UDGNdeI and reverse primer UDG-HindIII, 1 x Taq PCR buffer (New England Biolabs), 200 M each dNTP and 5 units of Taq DNA polymerase (New England Biolabs). The PCR procedure included a predenaturation step at 94◦ C for 3 min, 30 cycles of three-step a ...
Comprehensive Analysis of Hyrdrogen Bonds in Regulatory Protein
... of these bonds involve the protein side-chains and the DNA atoms at the base edges and in the backbone, but interactions that involve the protein backbone are also found. The contacts that involve the DNA backbone are believed to stabilize the complex and to orient the protein against the DNA in a f ...
... of these bonds involve the protein side-chains and the DNA atoms at the base edges and in the backbone, but interactions that involve the protein backbone are also found. The contacts that involve the DNA backbone are believed to stabilize the complex and to orient the protein against the DNA in a f ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... localized in the mitochondria. Therefore, pol-y is thought to be the enzyme that replicates the mitochondria1 DNA. The general enzymatic properties and characteristics that distinguish each DNA pol are summarized in Table 1, and the optimal assay conditions for each enzyme, such as pH, preferred pri ...
... localized in the mitochondria. Therefore, pol-y is thought to be the enzyme that replicates the mitochondria1 DNA. The general enzymatic properties and characteristics that distinguish each DNA pol are summarized in Table 1, and the optimal assay conditions for each enzyme, such as pH, preferred pri ...
Cyanogen bromide
... monomers of β-D-galactopyranose and 3,6-anhydro-α-L-galactopyranose). Cyanogen bromide activation is one of the most common methods for preparing affinity gels, and is useful because it reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates, which are attacked by prima ...
... monomers of β-D-galactopyranose and 3,6-anhydro-α-L-galactopyranose). Cyanogen bromide activation is one of the most common methods for preparing affinity gels, and is useful because it reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates, which are attacked by prima ...
Silver Biotics Information and Usage Guide for Your Reference What
... efficient and effective ways to design and engineer products. All silver is not created equal. Ionic Silver is one of the most common forms of silver liquid found in the supplement industry today. Many are made by diluting chemical forms of silver, like silver nitrate, to a desired parts per million ...
... efficient and effective ways to design and engineer products. All silver is not created equal. Ionic Silver is one of the most common forms of silver liquid found in the supplement industry today. Many are made by diluting chemical forms of silver, like silver nitrate, to a desired parts per million ...
Fingerprinting the Fungal Community
... are also separated by the ITS (internally transcribed spacer) regions, which are highly variable both in length and sequence composition. Fig 1 shows the arrangement of the fungal rRNA genes, together with the ITS regions. This pattern of rRNA subunit genes interspersed with ITS regions continues al ...
... are also separated by the ITS (internally transcribed spacer) regions, which are highly variable both in length and sequence composition. Fig 1 shows the arrangement of the fungal rRNA genes, together with the ITS regions. This pattern of rRNA subunit genes interspersed with ITS regions continues al ...
An improved technique for isolating codominant compound
... SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for succes ...
... SSR markers, this approach substantially reduced the cost of developing codominant markers and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for succes ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.