![Purification and Partial Characterization of a Latent Serine Protease](http://s1.studyres.com/store/data/022246065_1-a534944e387409e2794a3a9fdb4f9057-300x300.png)
Dynamic DNA nanotechnology using strand displacement reactions
... branch migration, or dissociation. Domains are represented here by numbers; a starred domain denotes a domain complementary in sequence to the domain without a star (e.g. domain 2∗ is complementary to domain 2). The sequences of the nucleotide bases are not typically shown because it is expected tha ...
... branch migration, or dissociation. Domains are represented here by numbers; a starred domain denotes a domain complementary in sequence to the domain without a star (e.g. domain 2∗ is complementary to domain 2). The sequences of the nucleotide bases are not typically shown because it is expected tha ...
Chemotaxis Assays for Marine and Freshwater Amoeba Jessica
... process has barely been studied in free-living environmental amoeba, which would undoubtedly also benefit from this fitness advantage while living in such heterogeneous environments. One study on the soil-amoeba Dictyostelium discoideum suggested a strong chemotactic response towards folate, however ...
... process has barely been studied in free-living environmental amoeba, which would undoubtedly also benefit from this fitness advantage while living in such heterogeneous environments. One study on the soil-amoeba Dictyostelium discoideum suggested a strong chemotactic response towards folate, however ...
Fusobacterium pseudonecrophorurn Is a Synonym for Fusobacten
... M NaCl containing 5 mM HEPES (N-2-hydroxyethylpiperazine-N'-Zethanesulfonic acid) buffer (pH 7.0), and 25 p1 of deionized formamide. These vials were incubated at 50°C for 20 h. The S1 nuclease procedure was used for DNA similarity determinations (8). Labeled reference DNA preparations reassociated ...
... M NaCl containing 5 mM HEPES (N-2-hydroxyethylpiperazine-N'-Zethanesulfonic acid) buffer (pH 7.0), and 25 p1 of deionized formamide. These vials were incubated at 50°C for 20 h. The S1 nuclease procedure was used for DNA similarity determinations (8). Labeled reference DNA preparations reassociated ...
Chapter 19: DNA Ligases - DNA Replication and Human
... The Saccharomyces cerevisiae CDC9 and Schizosaccharomyces pombe cdcl7+ gene products appear to be the yeast counterparts of mammalian DNA ligase I. Thus, the purified S. cerevisiae and S. pombe enzymes have catalytic properties practically identical to those of the mammalian enzyme, whereas they dif ...
... The Saccharomyces cerevisiae CDC9 and Schizosaccharomyces pombe cdcl7+ gene products appear to be the yeast counterparts of mammalian DNA ligase I. Thus, the purified S. cerevisiae and S. pombe enzymes have catalytic properties practically identical to those of the mammalian enzyme, whereas they dif ...
Protein Structure Analysis - G
... into their constituent’s subunits or polypeptides to examine the structure as well as composition of protein molecules. Sodium dodecyl sulfate (SDS) is commonly used for denaturing proteins into their constituent subunits or polypeptides and the method is known as sodium dodecyl sulfate (SDS)-polyac ...
... into their constituent’s subunits or polypeptides to examine the structure as well as composition of protein molecules. Sodium dodecyl sulfate (SDS) is commonly used for denaturing proteins into their constituent subunits or polypeptides and the method is known as sodium dodecyl sulfate (SDS)-polyac ...
Alteration by site-directed mutagenesis of the
... The final preparation obtained after chromatography on hydroxylapatite and heparin-agarose contained the RecB-K29Q, RecC, and RecD proteins, and a single major contaminant (Fig. 2). Analysis of the gel shown in Fig. 2 by densitometry showed that the contaminant was about 70% of the total protein in ...
... The final preparation obtained after chromatography on hydroxylapatite and heparin-agarose contained the RecB-K29Q, RecC, and RecD proteins, and a single major contaminant (Fig. 2). Analysis of the gel shown in Fig. 2 by densitometry showed that the contaminant was about 70% of the total protein in ...
Purification and characterization of the 1-3
... Molecular weight of the native enzyme was determined by two methods: 1. Gel filtration on a FPLC column of superose 12 HR 10/30 equilibrated with 50 mM KPB (pH 7.4) containing 100 mM KCl. Column was calibrated with known molecular weight standards (Da) (Sigma, St. Louis, MO, USA): blue dextran (2 00 ...
... Molecular weight of the native enzyme was determined by two methods: 1. Gel filtration on a FPLC column of superose 12 HR 10/30 equilibrated with 50 mM KPB (pH 7.4) containing 100 mM KCl. Column was calibrated with known molecular weight standards (Da) (Sigma, St. Louis, MO, USA): blue dextran (2 00 ...
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof
... (bright) and low (dark) quantum yields. To characterize the fluorescence properties of 2AP in dsDNA, we annealed a 15-nucleotide complementary oligonucleotide to 2AP-ssDNA. To confirm hybridization, we labeled the complementary strand with 3′-Cy3 (Cy3cDNA, Figure 2c, Table S1 in the Supporting Informa ...
... (bright) and low (dark) quantum yields. To characterize the fluorescence properties of 2AP in dsDNA, we annealed a 15-nucleotide complementary oligonucleotide to 2AP-ssDNA. To confirm hybridization, we labeled the complementary strand with 3′-Cy3 (Cy3cDNA, Figure 2c, Table S1 in the Supporting Informa ...
CHAPTER 13 DNA manipulation
... One class of enzymes can cut double-stranded DNA into a reproducible number of fragments. These enzymes are called restriction enzymes or cutting enzymes. These cutting enzymes occur naturally in microbes, mainly in bacteria, but also in some archaea. The main feature of cutting enzymes is that they ...
... One class of enzymes can cut double-stranded DNA into a reproducible number of fragments. These enzymes are called restriction enzymes or cutting enzymes. These cutting enzymes occur naturally in microbes, mainly in bacteria, but also in some archaea. The main feature of cutting enzymes is that they ...
Compiling DNA strand displacement reactions using a functional
... DNA strand displacement [7] is a robust mechanism for engineering sequence-specific interactions between DNA molecules. As shown in Figure 1a, we use the secondary structure abstraction of DNA structure, which ignores the double helical structure and absolute positions of the molecules and represent ...
... DNA strand displacement [7] is a robust mechanism for engineering sequence-specific interactions between DNA molecules. As shown in Figure 1a, we use the secondary structure abstraction of DNA structure, which ignores the double helical structure and absolute positions of the molecules and represent ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.