![Heredity + Nucleic Acids](http://s1.studyres.com/store/data/001751443_1-38f5803ec6188b61456851ec5afa827c-300x300.png)
Heredity + Nucleic Acids
... start back at the cell. As it became more firmly established that all organisms were composed of cells, and all cells were derived from pre-existing cells, it became more and more likely that inheritance had to be a cellular phenomena. As part of their studies, cytologists (students of the cell) beg ...
... start back at the cell. As it became more firmly established that all organisms were composed of cells, and all cells were derived from pre-existing cells, it became more and more likely that inheritance had to be a cellular phenomena. As part of their studies, cytologists (students of the cell) beg ...
Protocol
... 6. The ratio of the 260 nm measurement to the 280 nm measurement indicates purity. Ratios of 1.8 to 2.1 are very pure. Lower ratios indicate possible protein contamination (or low pH in the solution used as a diluent for the spectrophotometric readings). Proteins absorb at 280 and nucleic acid (DNA, ...
... 6. The ratio of the 260 nm measurement to the 280 nm measurement indicates purity. Ratios of 1.8 to 2.1 are very pure. Lower ratios indicate possible protein contamination (or low pH in the solution used as a diluent for the spectrophotometric readings). Proteins absorb at 280 and nucleic acid (DNA, ...
Deoxyribonucleic Acid Base Composition of Acetic
... Catlin & Cunningham (1961) found all the strains of Neisseria they examined to have 49.5-51.5 yo (G + C), except for N . catarrhalis, which had 40.0-41.3 yo. They conclude that ‘the inclusion of “ catarrhalis ” strains in the genus Neisseria appears illogical from the evolutionary point of view.’ Th ...
... Catlin & Cunningham (1961) found all the strains of Neisseria they examined to have 49.5-51.5 yo (G + C), except for N . catarrhalis, which had 40.0-41.3 yo. They conclude that ‘the inclusion of “ catarrhalis ” strains in the genus Neisseria appears illogical from the evolutionary point of view.’ Th ...
Polymerase chain reaction (PCR) The polymerase chain reaction
... Multiplex-PCR: consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times ...
... Multiplex-PCR: consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times ...
RiboMAX(TM) Large Scale RNA Production Systems
... Optimal RNA yields depend on a high-quality DNA template. The Wizard® Plus SV Minipreps System (Cat.# A1470) and the PureYield™ Plasmid Midiprep System (Cat.# A2492) yield DNA suitable for transcription reactions. The DNA template must be free of RNase. If the presence of RNase is suspected, treat t ...
... Optimal RNA yields depend on a high-quality DNA template. The Wizard® Plus SV Minipreps System (Cat.# A1470) and the PureYield™ Plasmid Midiprep System (Cat.# A2492) yield DNA suitable for transcription reactions. The DNA template must be free of RNase. If the presence of RNase is suspected, treat t ...
Isolation of a UV Endonuclease from the
... the UV endonucleases of E. coli and Micrococcus luteus have been shown to be stimulated by, but not dependent upon Mg2+. Assays were routinely carried out for 15 rnin at 37 OC under yellow, non-photoreactivating light (550-700 nm). Reactions were started by adding the lysate or enzyme containing fra ...
... the UV endonucleases of E. coli and Micrococcus luteus have been shown to be stimulated by, but not dependent upon Mg2+. Assays were routinely carried out for 15 rnin at 37 OC under yellow, non-photoreactivating light (550-700 nm). Reactions were started by adding the lysate or enzyme containing fra ...
Extension Activity 1: Plasmid Mapping STUDENT MANU AL
... Note that the two 1000 bp fragments (undigested sample and sample digested with enzyme 2) might not run at exactly the same distance on an agarose gel. There is a small difference in migration if a fragment of the same size is uncut (circular), cut (linear) or uncut and twisted (supercoiled). Also f ...
... Note that the two 1000 bp fragments (undigested sample and sample digested with enzyme 2) might not run at exactly the same distance on an agarose gel. There is a small difference in migration if a fragment of the same size is uncut (circular), cut (linear) or uncut and twisted (supercoiled). Also f ...
Nucleic Acids
... A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair of pyrimidine bases is too small to account for the observed thickness, whereas a pair of purine bases is too large. Thus, according to the Watson–Crick ...
... A significant feature of Watson and Crick’s model is that no other base pairing is consistent with the observed thickness of a DNA molecule. A pair of pyrimidine bases is too small to account for the observed thickness, whereas a pair of purine bases is too large. Thus, according to the Watson–Crick ...
A BB B BB - AIMS Press
... quantification was performed with the dye RiboGreen, specific for ssRNA. The result showed that the detection limit of the established qPCR assay was 0.5 PFU/mL more sensitive then RT- PCR. ...
... quantification was performed with the dye RiboGreen, specific for ssRNA. The result showed that the detection limit of the established qPCR assay was 0.5 PFU/mL more sensitive then RT- PCR. ...
Chapter 1
... and gravitational forces, which are required to prevent evaporation into space, it may even be present in liquid form. This is important as the origin of life depends on a solvent. The reactive gases that can build complex molecules can also destroy them. However, when these complex compounds dissol ...
... and gravitational forces, which are required to prevent evaporation into space, it may even be present in liquid form. This is important as the origin of life depends on a solvent. The reactive gases that can build complex molecules can also destroy them. However, when these complex compounds dissol ...
INDIGO-BINDING DOMAINS IN CELLULASE MOLECULES
... residues seem to play a major role in binding indigo. Indigo is a hydrophobic compound (insoluble in water), and its aromatic rings may interact with the aromatic rings of Tyr, Trp and Phe, as well as with side chains of other non-polar amino acids, via the hydrophobic interactions. Another mechanis ...
... residues seem to play a major role in binding indigo. Indigo is a hydrophobic compound (insoluble in water), and its aromatic rings may interact with the aromatic rings of Tyr, Trp and Phe, as well as with side chains of other non-polar amino acids, via the hydrophobic interactions. Another mechanis ...
Structure and Physiological significance of lipid
... A method of screening recombinants for inserted DNA fragments. Using the plasmid pBR322, a piece of DNA is inserted into the unique PstI site. This insertion disrupts the gene coding for a protein that provides ampicillin resistance to the host bacterium. Hence, the chimeric plasmid will no longer s ...
... A method of screening recombinants for inserted DNA fragments. Using the plasmid pBR322, a piece of DNA is inserted into the unique PstI site. This insertion disrupts the gene coding for a protein that provides ampicillin resistance to the host bacterium. Hence, the chimeric plasmid will no longer s ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.