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pIVEX - ISBG
... Design PCR primers according to section 3.3.1. • PCR conditions Optimal reaction conditions depend on the template/ primer pairs and have to be calculated accordingly. • To avoid nonspecific products and misincorporation, try to keep cycle number as low as possible (⬍25). • To reduce the error rate ...
... Design PCR primers according to section 3.3.1. • PCR conditions Optimal reaction conditions depend on the template/ primer pairs and have to be calculated accordingly. • To avoid nonspecific products and misincorporation, try to keep cycle number as low as possible (⬍25). • To reduce the error rate ...
16_Lecture_Presentation
... various proteins within the white blood cells. • he came across a substance from the cell nuclei that had chemical properties unlike any protein, – -a much higher phosphorous content ...
... various proteins within the white blood cells. • he came across a substance from the cell nuclei that had chemical properties unlike any protein, – -a much higher phosphorous content ...
The trimethoprim-resistant dihydrofolate reductase associated with
... Molecular weight determinations. The subunit molecular weight of the purified R388 dihydrofolate reductase was determined by electrophoresis on 7% and 10% polyacrylamide gels containing 0.2% SDS (14). The gels were calibrated with insulin chain B (Mr 34OO), aprotinin (Mr 6500), cytochrorae C (Mr 12 ...
... Molecular weight determinations. The subunit molecular weight of the purified R388 dihydrofolate reductase was determined by electrophoresis on 7% and 10% polyacrylamide gels containing 0.2% SDS (14). The gels were calibrated with insulin chain B (Mr 34OO), aprotinin (Mr 6500), cytochrorae C (Mr 12 ...
molecular_general_theory_complete
... (which can only synthesize new DNA from the 5’ to 3’). As a result, the DNA of the lagging strand is replicated in a piecemeal fashion. The primase, which accompanies the holoenzyme, synthesizes RNA primers along the lagging strand every few hundreds of base pairs, which are then used as primers for ...
... (which can only synthesize new DNA from the 5’ to 3’). As a result, the DNA of the lagging strand is replicated in a piecemeal fashion. The primase, which accompanies the holoenzyme, synthesizes RNA primers along the lagging strand every few hundreds of base pairs, which are then used as primers for ...
dna replication
... (which can only synthesize new DNA from the 5’ to 3’). As a result, the DNA of the lagging strand is replicated in a piecemeal fashion. The primase, which accompanies the holoenzyme, synthesizes RNA primers along the lagging strand every few hundreds of base pairs, which are then used as primers for ...
... (which can only synthesize new DNA from the 5’ to 3’). As a result, the DNA of the lagging strand is replicated in a piecemeal fashion. The primase, which accompanies the holoenzyme, synthesizes RNA primers along the lagging strand every few hundreds of base pairs, which are then used as primers for ...
Promega Notes: Separate Isolation of Genomic DNA and Total RNA
... the basic SV System protocol, the separate purification of both DNA and RNA from the same sample can be easily processed. In situations where it is desirable to purify only genomic DNA, the RNA purification can be omitted and high quality genomic DNA can be isolated. These procedures do not involve ...
... the basic SV System protocol, the separate purification of both DNA and RNA from the same sample can be easily processed. In situations where it is desirable to purify only genomic DNA, the RNA purification can be omitted and high quality genomic DNA can be isolated. These procedures do not involve ...
The effect of human serum DNAases on the ability to detect
... count was performed by two independent observers; the number of bacteria obtained in the phase-contrast mode was taken as the total bacterial count and set at 100%. The percentage of bacteria that had released DNA was calculated as the difference between the total bacterial count and the number of b ...
... count was performed by two independent observers; the number of bacteria obtained in the phase-contrast mode was taken as the total bacterial count and set at 100%. The percentage of bacteria that had released DNA was calculated as the difference between the total bacterial count and the number of b ...
The polymerase chain reaction
... different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a ...
... different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a ...
DNA recognition code of transcription factors
... factor specifically contact bases and shows what residue sizes are compatible with these positions. From a fixed position on the interaction surface, a long side chain can reach further into the DNA major groove, while at another position which is very close to the DNA a small residue can easily fit ...
... factor specifically contact bases and shows what residue sizes are compatible with these positions. From a fixed position on the interaction surface, a long side chain can reach further into the DNA major groove, while at another position which is very close to the DNA a small residue can easily fit ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... a conformation that did not alter the overall B-form of the DNA helix. These spectra were also insensitive to the salt concentration and solvent composition investigated in this study (Figure S1). The CD spectral data suggested that the differences in thermodynamic stabilities among the duplexes res ...
... a conformation that did not alter the overall B-form of the DNA helix. These spectra were also insensitive to the salt concentration and solvent composition investigated in this study (Figure S1). The CD spectral data suggested that the differences in thermodynamic stabilities among the duplexes res ...
Protein_Synthesis_and_Words
... The X marked nucleotides are an example of a DNA sequence that would be used to code for a particular protein, with the sequence of these nucleotides determining which protein it is. The sequence of these nucleotides are used to create amino acids, where chains of amino acids form to make a protein. ...
... The X marked nucleotides are an example of a DNA sequence that would be used to code for a particular protein, with the sequence of these nucleotides determining which protein it is. The sequence of these nucleotides are used to create amino acids, where chains of amino acids form to make a protein. ...
Heredity + Nucleic Acids
... start back at the cell. As it became more firmly established that all organisms were composed of cells, and all cells were derived from pre-existing cells, it became more and more likely that inheritance had to be a cellular phenomena. As part of their studies, cytologists (students of the cell) beg ...
... start back at the cell. As it became more firmly established that all organisms were composed of cells, and all cells were derived from pre-existing cells, it became more and more likely that inheritance had to be a cellular phenomena. As part of their studies, cytologists (students of the cell) beg ...
Agarose gel electrophoresis
![](https://commons.wikimedia.org/wiki/Special:FilePath/DNAgel4wiki.png?width=300)
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.