DNA-Directed Antibody Immobilization for
... diameter of ∼100−120 μm, and antibody spots had a diameter of ∼150 μm. Antibody−DNA Conjugate Synthesis. Antibody−DNA conjugates were synthesized by using Thunder-Link Oligo Conjugation Kit (Innova Biosciences). The DNA concentration used in the reaction was optimized to yield 1−2 DNA sequences per ...
... diameter of ∼100−120 μm, and antibody spots had a diameter of ∼150 μm. Antibody−DNA Conjugate Synthesis. Antibody−DNA conjugates were synthesized by using Thunder-Link Oligo Conjugation Kit (Innova Biosciences). The DNA concentration used in the reaction was optimized to yield 1−2 DNA sequences per ...
Quick Ligation™ Kit
... work well. For optimum ligation, the volume of DNA and insert should be 10 µl before adding 2X Quick Ligation Buffer. For DNA volumes greater than 10 µl, increase the volume of 2X Quick Ligation Buffer such that it remains 50% of the reaction and correspondingly increase the volume of ligase. The ov ...
... work well. For optimum ligation, the volume of DNA and insert should be 10 µl before adding 2X Quick Ligation Buffer. For DNA volumes greater than 10 µl, increase the volume of 2X Quick Ligation Buffer such that it remains 50% of the reaction and correspondingly increase the volume of ligase. The ov ...
Ribosome Display: In Vitro Selection of Protein
... allowing the selection from very large combinatorial libraries. In add ition, the ropid selection cycles require an integra l polymerase cboin re«ction (PCR) step, which can be used for ra ndomization, making this method ideal for directed evolution experiments. The fact that the ribosomal complex u ...
... allowing the selection from very large combinatorial libraries. In add ition, the ropid selection cycles require an integra l polymerase cboin re«ction (PCR) step, which can be used for ra ndomization, making this method ideal for directed evolution experiments. The fact that the ribosomal complex u ...
Isolate and Purify Phage Genomic DNA
... until you are sure that the phage precipitant solution is completely mixed with the lysate. Look at the tube near a source of light so you can be sure that the solution is homogeneously mixed. This mixing step is important for efficient precipitation. If you see schlierin lines (visible streaks prod ...
... until you are sure that the phage precipitant solution is completely mixed with the lysate. Look at the tube near a source of light so you can be sure that the solution is homogeneously mixed. This mixing step is important for efficient precipitation. If you see schlierin lines (visible streaks prod ...
Isolating, Cloning, and Sequencing DNA
... that is required to make protein molecules move uniformly toward the positive electrode. For DNA fragments less than 500 nucleotides long, specially designed polyacrylamide gels allow separation of molecules that differ in length by as little as a single nucleotide (Figure 8-23A). The pores in polya ...
... that is required to make protein molecules move uniformly toward the positive electrode. For DNA fragments less than 500 nucleotides long, specially designed polyacrylamide gels allow separation of molecules that differ in length by as little as a single nucleotide (Figure 8-23A). The pores in polya ...
Nucleic Acids
... synthesis of two or more polypeptide chains). Since the ribosomes bind the mRNA and initiate translation at defined positions (the ribosome-binding sites), translation may begin from more than one site on a single molecule of mRNA. As suggested above, mRNAs can be quite variable in size. mRNAs are a ...
... synthesis of two or more polypeptide chains). Since the ribosomes bind the mRNA and initiate translation at defined positions (the ribosome-binding sites), translation may begin from more than one site on a single molecule of mRNA. As suggested above, mRNAs can be quite variable in size. mRNAs are a ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... appeared within 2 min. When the acid-unfolded DMSOR has been incubated in refolding buffer (pH 8.0) at 20°C for 2 h, it became almost undetectable after electrophoresis on a non-denaturing gel. This result suggests that the acid-unfolded DMSOR might have aggregated after incubation. The aggregation ...
... appeared within 2 min. When the acid-unfolded DMSOR has been incubated in refolding buffer (pH 8.0) at 20°C for 2 h, it became almost undetectable after electrophoresis on a non-denaturing gel. This result suggests that the acid-unfolded DMSOR might have aggregated after incubation. The aggregation ...
Biology Review
... protein. This type of structure are maintained by : Covalent, ionic bonds between amino acid R groups Quaternary structure- If the protein is made up of more than one polypeptide chain i.e. hemoglobin It is critical that proteins have a certain structure (structure function relationship) It is cruci ...
... protein. This type of structure are maintained by : Covalent, ionic bonds between amino acid R groups Quaternary structure- If the protein is made up of more than one polypeptide chain i.e. hemoglobin It is critical that proteins have a certain structure (structure function relationship) It is cruci ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.