EPICENTRE Enzyme Catalog
... are resistant to RNase A-type RNases. T7 & SP6 R&DNA™ Polymerases, & DuraScribe™ T7 & SP6 Transcription Kits to synthesize nucleic acids with non-canonical bases or for partial ribosubstitution are covered by U.S. Patents 5,849,546; 6,107,037; or 6,596,494, and other patents issued or pending. These ...
... are resistant to RNase A-type RNases. T7 & SP6 R&DNA™ Polymerases, & DuraScribe™ T7 & SP6 Transcription Kits to synthesize nucleic acids with non-canonical bases or for partial ribosubstitution are covered by U.S. Patents 5,849,546; 6,107,037; or 6,596,494, and other patents issued or pending. These ...
Chapter 2 - University of the Free State
... •Larger molecules cannot migrate through small gel pores •Percentage acrylamide has range within which molecules can be separated •3% gels for protein of 1000kDa •20% gels for proteins of 10kDa •Polyacrylamide gel electrophoresis = PAGE ...
... •Larger molecules cannot migrate through small gel pores •Percentage acrylamide has range within which molecules can be separated •3% gels for protein of 1000kDa •20% gels for proteins of 10kDa •Polyacrylamide gel electrophoresis = PAGE ...
The nucleotide sequence and derived amino acid
... have a different residue at this position in the other two isozymes. For example, all ofthe CA II isozymes have His residues at positions 2 and 3 (as does the mouse CA isozymv), whereas the His does not appear at these positions in any of the CA I or CA III isozymes. This information is summarized i ...
... have a different residue at this position in the other two isozymes. For example, all ofthe CA II isozymes have His residues at positions 2 and 3 (as does the mouse CA isozymv), whereas the His does not appear at these positions in any of the CA I or CA III isozymes. This information is summarized i ...
Chemical Synthesis of Oligonucleotides
... mass of full-length product will be sufficient to produce the amplicon of interest in an equally overwhelming mass. Thus, for PCR, there is little need for purification beyond that of desalting particularly with the very high quality of oligos that IDT maintains. IDT also offers standard desalting f ...
... mass of full-length product will be sufficient to produce the amplicon of interest in an equally overwhelming mass. Thus, for PCR, there is little need for purification beyond that of desalting particularly with the very high quality of oligos that IDT maintains. IDT also offers standard desalting f ...
NUCLEOTIDES AND NUCLEIC ACIDS
... joined to the 3-hydroxyl group of the next nucleotide, creating a phosphodiester linkage (Fig. 8–7). Thus the covalent backbones of nucleic acids consist of alternating phosphate and pentose residues, and the nitrogenous bases may be regarded as side groups joined to the backbone at regular interva ...
... joined to the 3-hydroxyl group of the next nucleotide, creating a phosphodiester linkage (Fig. 8–7). Thus the covalent backbones of nucleic acids consist of alternating phosphate and pentose residues, and the nitrogenous bases may be regarded as side groups joined to the backbone at regular interva ...
Identification
... After amplification, 10 µL from the cycled reactions is mixed with 2 µL of loading dye (25µg bromophenol blue and 25 µg xylene cyanol FF in 10 mL 50 % glycerol) and amplification products are resolved by electrophoresis on a 1.5 % agarose gel made with 1X TBE buffer at pH 8.0 (9.0 mM Tris, 8.9 mM bo ...
... After amplification, 10 µL from the cycled reactions is mixed with 2 µL of loading dye (25µg bromophenol blue and 25 µg xylene cyanol FF in 10 mL 50 % glycerol) and amplification products are resolved by electrophoresis on a 1.5 % agarose gel made with 1X TBE buffer at pH 8.0 (9.0 mM Tris, 8.9 mM bo ...
Copy of NAR30_7.book(gkf263.fm)
... displace the alkylthiol-capped oligonucleotides from the Au nanoparticle surface (19). To increase the stability of the DNA–nanoparticle conjugates, we have previously developed a cyclic dithiane-epiandrosterone anchor group capped oligonucleotides such as 2 (Scheme 1B), which leads to significantly ...
... displace the alkylthiol-capped oligonucleotides from the Au nanoparticle surface (19). To increase the stability of the DNA–nanoparticle conjugates, we have previously developed a cyclic dithiane-epiandrosterone anchor group capped oligonucleotides such as 2 (Scheme 1B), which leads to significantly ...
The DnaE polymerase from Deinococcus radiodurans features
... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
... (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E. coli RNA polymerase, from which αDr could not be dissociated. On the contrary, when refolded from inc ...
Diagnostic protocol for
... Aliquots of 25 µl of each bacterial preparation or plant samples to be tested are pipetted onto the windows of a plastic-coated multiwindow microscope slide, allowed to air-dry and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative cont ...
... Aliquots of 25 µl of each bacterial preparation or plant samples to be tested are pipetted onto the windows of a plastic-coated multiwindow microscope slide, allowed to air-dry and gently heat-fixed over a flame. Separate slides are set up for each test bacterium and also, positive and negative cont ...
Strategies for Attaching Oligonucleotides to Solid Supports
... properties have been well studied and is amenable to chemical modification using very versatile and well developed silanization chemistry [1]. Most attachment protocols involve chemically modifying the glass surface to facilitate attachment of the oligo. Silianized oligonucleotides can also be coval ...
... properties have been well studied and is amenable to chemical modification using very versatile and well developed silanization chemistry [1]. Most attachment protocols involve chemically modifying the glass surface to facilitate attachment of the oligo. Silianized oligonucleotides can also be coval ...
Molecular Evolution of Functional Nucleic Acids
... Perrin et al. obtained a modified DNA enzyme from a library of doubly modified DNA involving both a deoxyuridine analog with allylamine at the 5 position and a deoxyadenosine analog with histamine at the 8 position, instead of the corresponding natural nucleotides [24]. Interestingly, the 20 bases o ...
... Perrin et al. obtained a modified DNA enzyme from a library of doubly modified DNA involving both a deoxyuridine analog with allylamine at the 5 position and a deoxyadenosine analog with histamine at the 8 position, instead of the corresponding natural nucleotides [24]. Interestingly, the 20 bases o ...
JOIN2004 Universidade do Minho
... Regular expressions are a language within the Perl language that describe many kinds of patterns in strings, e.g. motifs in DNA. ...
... Regular expressions are a language within the Perl language that describe many kinds of patterns in strings, e.g. motifs in DNA. ...
Agarose gel electrophoresis
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix.Agarose gels are easy to cast and are particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. Most agarose gels used are between 0.7 - 2% dissolved in a suitable electrophoresis buffer.