Download High throughput nucleic acid sample preparation in 96 well plates

High throughput nucleic acid
sample preparation in 96 well plates
CONTENT
01//
02//
03//
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Product Selection Guide
2
High Throughput Purification Procedures
4
GENOTYPING
Invisorb® Blood Mini HTS 96 Kit
5
Invisorb® DNA Tissue HTS 96 Kit
7
PSP® SalivaGene DNA HTS 96 Kit
8
PATHOGEN DETECTION
Invisorb® Virus RNA HTS 96 Kit
10
Invisorb® Universal Bacteria HTS 96 Kit
11
AGRICULTURAL AND FOOD APPLICATIONS (GMO)
Invisorb® and InviTrap® Plant HTS 96 Kits
12
GENE EXPRESSION ANALYSIS
InviTrap® RNA Cell HTS 96 Kit
13
DNA FRAGMENT PURIFICATION
MSB® HTS PCRapace
14
Kit Overview
15
Ordering Information
16
1
Smarter Nucleic Acid Sample Preparation
STRATEC Molecular offers a broad range of products for high throughput applications, which are developed for highly efficient purification of DNA and RNA from a wide
range of starting materials. All kits are optimized for use on a centrifuge with a deep-well
rotor. Many kits can also be used with a vacuum manifold or on robotic workstations. For
adaption to robotic platforms please contact us ([email protected]) or your local sales
representative.
STRATEC Molecular developed a proprietary filter plate with a 1 ml working volume dedicated
to nucleic acid purification in 96 well format. The long drip directors guarantee the prevention
of well to well contamination during the purification process, and a reduction of aerosol development. In addition, this technology leads to a reduction of foam development if detergent
containing buffers are used. Additionally, the plates incorporate a unique one-piece design to
eliminate leaking and allow for extended incubations. Furthermore tear-resistant membranes ensure safe and reliable DNA/RNA purification. The plates are automation compatible to
provide maximum sample preparation throughput.
Long years of experience in kit development and reagent optimization in combination with a
thorough understanding of requirements and limitations in automation have resulted in highly optimized solutions for your automated nucleic acid extraction needs.
With our kits you can:
Improve Efficiency
Maximize Capabilities
Improve Safety
Improve Effectiveness
Increase Environmental-friendliness
Streamline your sample preparation process by using STRATEC Molecular products.
www.invitek.de
2
PRODUCT SELECTION GUIDE
NUCLEID ACID
TYPE
STARTING MATERIAL
RECOMMENDED KIT
DNA
Genomic
Blood, buffy coat, bone
marrow, swabs
Invisorb® Blood Mini HTS 96 Kit
InviMag® & Invisorb® Universal Kit
Tissue, rat or mouse tail,
cryosections, food
Invisorb® DNA Tissue HTS 96 Kit
Cells (eucaryotic)
Invisorb® DNA Tissue HTS 96 Kit
Saliva, swabs
PSP® SalivaGene DNA HTS 96 Kit
Plants (leaves, roots, fruits,
flowers, wood, oily seeds,
water plants, food)
Invisorb® DNA Plant HTS 96 Kit
Bacterial
Bacteria pellets,
tissue, swabs
cell-free body fluids, blood,
urine, food samples
Invisorb® Universal Bacteria HTS 96 Kit
InviMag® & Invisorb® Universal Kit
Viral
Blood, serum, plasma,
cell-free body fluids,
cell culture supernatants, swabs,
eucaryotic cells
Invisorb® Virus DNA HTS 96 Kit
InviMag® & Invisorb® Universal Kit
DNA
fragments
PCR products, amplification
reaction mixture, cDNA synthesis,
ligation mixtures,
restriction digestion mixtures
MSB® HTS PCRapace
Viral
Serum, plasma,cell culture
supernatants, swabs,
stool samples
Invisorb® Virus RNA HTS 96 Kit
InviMag® & Invisorb® Universal Kit
Total
Cells (eucaryotic)
InviTrap® RNA Cell HTS 96 Kit
Tissue
InviTrap® RNA Tissue HTS 96 Kit
Plants (leaves, roots, fruits,
flowers, wood, oily seeds,
water plants, food)
InviTrap® RNA Plant HTS 96 Kit
RNA
STA - STARlet from Hamilton Robotics
X
- X-tractor Gene from Corbett Robotics
ep
- epMotion 5075 Vac from Eppendorf
Tec
- Freedom Evo fom Tecan Group
Adaptions to other liquid handling platforms may be performed upon request.
Magnetic bead based applications for use on KingFisher 96 and Flex are also available.
3
SAMPLE VOLUME
YIELD / WELL
CENTRIFUGE
VACUUM
MANIFOLD
Up to 200 µl
Up to 10 µg
x
x
Up to 20 mg
0.2 - 1.2 μg per mg tissue
10 - 35 μg per mouse tail (1.0 cm)
20 - 40 μg per rat tail (0.5 cm)
Up to 106 cells
AUTOMATED
PROTOCOLS
AVAILABLE FOR
PAGE
STA**, X, ep***
05
x
Tec
07
Up to 6 - 10 µg
x
Tec
07
500 - 800 µl
Depending on starting
material
x
ep***
08
Up to 50 mg
Up to 6 µg
x
Up to 109 cells
up 100 µl liquid sample
up to 10 mg tissue
up to 25 g food sample
15 - 50 ml urine
Depending on starting
material
x
x
STA**,X, ep***
Up to 200 µl
up to 106 cells
Depending on viral titer
x
x
ep, X, STA**,
X, ep***
Up to 100 µl
Up to 95 % recovery
x
x
Up to 200 µl or 50 mg
Depending on viral titer
x
x
Up to 5 x 105 cells
Up to 20 µg
x
x
Up to 10 mg
Up to 30 µg
x
*
Up to 50 mg
Up to 30 µg
x
*
x
12
11
*
14
STA**,X, ep***
10
13
*) Please find more information online at www.invitek.de
**) Please request specific brochure about Kits on STARlet from Hamilton Robotics
***) limited support
4
HIGH THROUGHPUT PURIFICATION PROCEDURES
3. Total RNA isolation
1.
1. Genomic DNA isolation
2. DNA/RNA isolation
from pathogens
1.
1.
2.
3.
2.
4. DNA fragment
purification
2.
3.
1.
4.
3.
4.
2.
5.
4.
3.
6.
5.
5.
(Prelysis of cell wall)
1. Lysis of starting
material
1. Lysis of starting
material
2. Adjustment of
binding conditions
2. Adjustment of
binding conditions
3. Binding of genomic
DNA
3. Binding of bacterial
and viral DNA/RNA
4. Washing and ethanol
removal
4. Washing and ethanol
removal
5. Elution of genomic
DNA
5. Elution of bacterial
and viral DNA/RNA
1. Cell lysis
Adjustment of DNA
binding conditions
2. Selective DNA
binding and removal
3. Adjustment of RNA
binding conditions
4. Binding of total RNA
5. Washing and ethanol
removal
6. Elution of total RNA
1. Adjustment of
binding conditions
2. Binding of
DNA fragments
3. Elution of
DNA fragments
5
Genotyping
DNA isolation from blood and body fluids
The Invisorb® Blood Mini HTS 96 Kit is designed for genomic DNA purification from human
and animal blood as well as from buffy coat, bone marrow or rinse liquid from swabs. Fresh
or frozen whole blood treated with EDTA or citrate, from routine blood collection systems can
be used. The kit is CE-marked in compliance with the Directive 98/79/EC on in vitro diagnostic
medical devices (IVD-Directive)*
EASY PROCEDURE
Purify up to 10 µg genomic DNA from 96 samples in parallel with no additional erythrocyte lysis in approx. one hour
COMPATIBLE WITH A VARIETY OF ANTICOAGULANTS AND TUBES
For human blood, mammalian and non-mammalian animal blood, rinse liquid from
swabs (max. 200 µl), buffy coat (max. 30 µl), bone marrow (20 µl), compatible with EDTA
and citrate
EFFECTIVE FOR A VARIETY OF DOWNSTREAM APPLICATIONS
Suitable for applications based on enzymatic amplification and detection of DNA
(PCR and real-time PCR, SNP analysis, genotyping etc.)
Fig. 1: AUTOMATED REPRODUCIBLE DNA ISOLATION ON THE epMotion® 5075 VAC
A
Genomic DNA from 40 blood samples was isolated using the Invisorb® Blood Mini HTS 96 Kit on the epMotion®
5075 VAC from Eppendorf. The procedure consistently delivered high molecular weight DNA as indicated by clear
bands without detectable RNA contamination (A). The DNA was suitable for PCR amplification which is demonstrated by the successful amplification of the GAPDH-sequence in the samples (B).
B
01
6
RT-PCR
Fig. 2: Genomic DNA isolation from blood samples and amplification
1K-ras qPCR
Genomic DNA was isolated from 24 samples of 200 µl transfusion blood using the InviMag® Universal Kit/ STARlet. 2.5 µl of isolated DNA
were amplified using an in-house assay for K-ras. The UV/VIS of 8 replicates in figure 1 shows the reproducible high quality of DNA.
Ratio A260/A280 (24 samples): 1.72 SD: 0.05
Ratio A260/A230 (24 samples): 1.65 SD: 0.35
Yield: 3.99 µg (24 samples):
SD: 0.46
References:
Short Communication: Association Analysis of Microsatellites and Mycobacterium avium
Subspecies paratuberculosis Antibody Response in German Holsteins.
M. Hinger, H. Brandt, S. Horner, and G. Erhardt
J Dairy Sci, Apr 2007; 90: 1957 - 1961.
Epidemiological and genetical differences between classical and atypical scrapie cases.
Lühken et al., 2007, Vet. Res. 38 (2007) 65–80
Association study in naturally infected helminth layers shows evidence for influence of
interferon-gamma gene variants on Ascaridia galli worm burden.
Lühken G, Gauly M, Kaufmann F, Erhardt G.
Vet Res. 2011 Jul 12;42(1):84.
PRODUCT
Invisorb® Blood Mini HTS 96 Kit/ C
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7031300200
24 x 96 purifications
7031300400
4 x 96 purifications
7031300300
Please find ordering information for vacuum based or automated kits on page 16.
For purification of genomic DNA from blood the InviMag®Blood or Universal Kit/ STARlet and Invisorb® Blood or Universal HTS 96 Kit/
STARlet are available. Please request the info material.
*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
7
Genotyping
DNA isolation from tissue samples and cells
The Invisorb® DNA Tissue HTS 96 Kit enables genomic DNA purification from a variety of tissue types incl. mouse and rat tails, cryosections, food and from eucaryotic cells. PCR inhibitors,
which can interfere with the amplification reaction, are efficiently removed. Pure DNA can be
used in subsequent downstream applications, such as PCR, real-time PCR, SNP detection, sequencing and cloning. The kit is CE-marked in compliance with the Directive 98/79/EC on in
vitro diagnostic medical devices (IVD-Directive)*.
OPTIMIZED PROTOCOLS FOR A VARIETY OF STARTING MATERIALS
For various tissue specimens (max. 20 mg), rodent tails, eucaryotic cells (max. 106)
MORE INTACT DNA
No DNA degradation by using a gentle, low-salt (non-chaotropic) buffer system
REPRODUCIBLE AMPLIFICATION RESULTS
Complete removal of contaminants and inhibitors
DNA from different stickleback eggs and spins was
isolated using the Invisorb® DNA Tissue HTS 96
Kit on Freedom Evo 200 workstation from Tecan.
Purified DNA from different samples was used as
template for Multiplex PCR using 5 microsatellites
targets.
Data kindly provided by Mr. Eizaguirre from
Department of Evolutionary Ecology, Max Planck
Offspring 2
Offspring 1
Father
Mother
Fig. 3: PATERNITY TESTING BY ISOLATION OF DNA FROM FISH EGGS AN SPINES
PRODUCT
Invisorb® DNA Tissue HTS 96 Kit/ C
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7032300200
24 x 96 purifications
7032300400
4 x 96 purifications
7032300300
Please find ordering information for vacuum based or automated kits on page 16.
*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
01
8
Genotyping
DNA sample preparation from saliva and swabs
The SalivaGene® system combines the use of the novel SalivaGene® Collector or the Saliva
Gene® Buccal Swab for saliva and swab sample collection and subsequent DNA purification.
The SalivaGene® Collector and the SalivaGene® Buccal Swab tubes are pre-filled with a DNA
stabilization buffer which eliminates the need of sample cooling and stabilizes genomic DNA
for 12 months at room temperature. The non-invasive saliva and swab collection methods
reduce puncture-associated infection risks and provide high quality DNA performing as well as
blood-derived DNA in downstream applications. For high throughput applications DNA purification can be completed using the PSP® SalivaGene DNA HTS 96 Kit.
HIGHEST DNA YIELDS
High quality DNA suitable for various downstream applications e.g. genotyping and
genomic sequencing, pharmacogenomics
LONG-TERM ROOM TEMPERATURE STABILITY OF SAMPLES
Less costs and sample management during transport and storage without refrigeration
PAINLESS SELF-COLLECTION
Pre-filled sample collection tubes for easy sample collection with DNA stabilization
buffer – no medical personnel required
Fig. 4: SalivaGene® COLLECTOR
9
Genotyping
Fig. 5: AUTOMATED DNA ISOLATION FROM STABILIZED SWAB SAMPLES
01
A
B
Genomic DNA was isolated using the PSP® SalivaGene DNA HTS 96 Kit on the epMotion® 5075 VAC (Eppendorf AG, Hamburg, Germany) from SalivaGene® stabilized swabs from 8 different donors stored at room temperature for six weeks. 10 µl of the eluted DNA were
analyzed on a 1.5 % agarose gel stained with ethidium bromide (A). The DNA quantity ranged from 1.5 – 6 µg. The GAPDH sequence was
amplified in a real-time PCR using the StepOnePlus™ Real-Time PCR System from Applied Biosystems (B). 2.5 µl of the eluted DNA were
used as template.
(Yellow lane – negative control; green lane: positive control)
Fig. 6: BIOLOGICAL VARIABILITY OF SALIVA SAMPLES
53 different saliva samples of randomly selected volunteers were extracted after stabilization for 2 – 4 weeks at room temperature.
500 µl of stabilized saliva was used for manual extraction and were measured by photometric analysis. An average DNA yield of about
25 µg / 500 µl sample was obtained. Data are shown in comparison to typical theoretical data of DNA obtained from 200µl of EDTA
stabilized blood (typical yield from 4 to 12 µg). The high standard deviation is due to the variability of the collected samples reflecting
biological variability of individuals as well as sampling variability.
PRODUCT
PSP® SalivaGene DNA HTS 96 Kit / C
SalivaGene® Collector
SalivaGene® Buccal Swab
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7035360200
24 x 96 purifications
7035360400
4 x 96 purifications
50 pieces
50 pieces
Please find ordering information for vacuum based or automated kits on page 16.
7035360300
1035210200
1035230200
10
Pathogen Detection
Viral RNA from clinical samples
Use the Invisorb® Virus RNA HTS 96 Kit for isolation of high-quality RNA from viruses found in
a diverse range of starting materials from 96 samples in parallel. The kit simplifies viral RNA
isolation by combining efficient lysis and the use of Carrier RNA for the enhancement of RNA
recovery in samples with low viral titer, allowing for sensitive virus detection in qualitative and
quantitative assays. The kit is CE-marked in compliance with the Directive 98/79/EC on in vitro
diagnostic medical devices (IVD-Directive)*.
RELIABLE PROCEDURE
RT-PCR templates in one hour with high sensitivity at low virus titers
FOR VARIOUS SAMPLE TYPES
Plasma, serum, cell culture supernatants, rinse liquid from swabs (max. 200 µl), stool
samples (max. 50 mg)
COMPLETE REMOVAL OF PCR INHIBITORS
Pure viral RNA for downstream analysis, e.g. real-time RT-PCR
Fig. 7: NOROVIRUS RNA ISOLATION FROM HUMAN STOOL SAMPLES
A
B
qRT-PCR of Norovirus genogroup I (FAM channel (A)) and Norovirus genogroup II (ROX channel (B)). Amplification plots of normalized
fluorescence versus the PCR cycle numbers are displayed for manually extracted RNA specimens (red plots) and automatically extracted
RNA specimens using the Invisorb® Virus RNA HTS 96 Kit/ X (green or blue plots) on the X-tractor™ Gene. Plots of Norovirus genogroup I
and II-negative specimens, respectively, are below the threshold.
Data kindly provided by C. Helmeke, H-M., Irmscher, State Office of Consumer Protection, Saxony-Anhalt, Department Medical Microbiology, Magdeburg, Germany
PRODUCT
Invisorb® Virus RNA HTS 96 Kit/ C
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7043300200
24 x 96 purifications
7043300400
4 x 96 purifications
7043300300
Please find ordering information for vacuum based or automated kits on page 16.
For DNA purification from DNA viruses the Invisorb® Virus DNA HTS 96 Kit (7042300200) is also available
For purification of viral DNA or RNA the InviMag® Universal Kit/ STARlet, Invisorb® Universal HTS 96 Kit/ STARlet and for purification
of viral RNA the Invisorb® Virus RNA HTS 96 Kit/ STARlet or InviMag® Virus RNA Kit/ STARlet are available.
Please request the info material.
*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
11
Pathogen Detection
Bacterial DNA from different sample types
The Invisorb® Universal Bacteria HTS 96 Kit is designed for genomic DNA isolation from different bacteria species from a broad range of starting materials. Pure DNA can be used in subsequent downstream applications, such as PCR, real-time PCR, restriction analysis, and sequencing.
FOR A WIDE VARIETY OF SAMPLES
Bacteria cell pellets (max. 109), blood, cell-free body fluids, rinse liquid from swabs
(max 100 µl), tissue (max. 10 mg), food samples (max. 25 g), urine (15 - 50 ml)
RELIABLE PROCEDURE
PCR templates in approx. 80 minutes with high sensitivity at low bacterial titer
REPRODUCIBLE AMPLIFICATION RESULTS
Complete removal of contaminants and inhibitors
Fig. 8: HIGHLY REPRODUCIBLE AND SENSITIVE ISOLATION OF BACTERIAL DNA
A
B
Real-time PCR results using DNA extracted from a dilution series of Bacillus subtilis cells in TE buffer (A). The melting curve for the resulting PCR products is shown (B). NTC (no template control, black) and PTC (positive template control, pink) are also shown. The efficiency
of the extraction kit and detection system leads to positive amplification results, if the starting material includes > 100 bacterial cells
(5 copies per PCR reaction).
PRODUCT
Invisorb® Universal Bacteria HTS 96 Kit/ C
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7033300200
24 x 96 purifications
7033300400
4 x 96 purifications
7033300300
Please find ordering information for vacuum based or automated kits on page 16
For purification of bacterial DNA the InviMag® Universal Kit/ STARlet or Invisorb® Universal HTS 96 Kit/STARlet are also available.
Please request the info material.
02
12
Agricultural and Food Applications (GMO)
DNA and RNA isolation from plants
The Invisorb® DNA Plant HTS 96 Kit or the InviTrap® RNA Plant HTS 96 Kit are effective solutions for isolation of high-quality total cellular DNA or total RNA from a wide variety of plant
species and tissue types. Furthermore the kits are is suitable for co-purification of DNA or RNA
from phytopathogens. Up to 50 mg of fresh or frozen material can be processed in one hour.
The isolated, pure DNA can be used in subsequent downstream applications such as PCR, restriction analysis, real-time PCR, cloning, genotyping etc. The high pure RNA can be used in
subsequent downstream applications such as RT-PCR, real-time RT-PCR,Northern Blot analysis,
microarray analysis ...etc.
HIGH YIELDS AND PURITY
Up to 6 µg/ well DNA, free of polysaccharides and other secondary metabolites
03
DNA FREE RNA WITHOUT DNASE DIGESTION
Selective removal of DNA during lysis
FOR VARIOUS PLANT MATERIALS AND FOOD (GMO)
Leaves, roots, fruits, flowers, wood, oily seeds, water plants
Fig. 9: DNA ISOLATION FROM ARABIDOPIS THALIANA
AND SUBSEQUENT PCR AMPLIFICATION
Fig. 10: DNA FREE RNA FROM HYBRID ASPEN LEAFS WITHOUT DNASE
TREATMENT
DNA was isolated from single leaves from Arabidopsis
thaliana using the Invisorb® DNA Plant HTS 96 Kit on a
deep well rotor centrifuge. Leaves were homogenized
before lysis by using a Retsch Mixer mill under liquid
nitrogen. The DNA was eluted in 60 µl and 5 µl were
used in a subsequent PCR reaction. 10 µl of the PCR
products were analyzed on a 2% agarose gel.
Figure 10 demonstrates the effective removal of genomic DNA during the
isolation of total RNA without DNase treatment from hybrid aspen plant
leafs using InviTrap® Plant RNA HTS 96 Kit and a competitor’s kit. Here
RNA was DNase or left without treatment. Heat-shock either subjected to
genes were either induced (I) or not (C). Samples were analyzed for heatshock gene expression via reverse- transcript respective signals ion PCR.
Only induced samples should display the respective signals. The presences
of bands in control sample lanes indicate inefficient removal of genomic
DNA.
PRODUCT
Invisorb® DNA Plant HTS 96 Kit/ C
InviTrap® RNA Plant HTS 96 Kit/ C
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7037300200
24 x 96 purifications
7037300400
4 x 96 purifications
4 x 96 purifications
24 x 96 purifications
For RNA purification from plants the InviTrap® RNA Plant HTS 96 Kit (7064300300) is also available.
7037300300
7064300300
7064300400
13
Gene Expression Analysis
RNA purification from cells
The InviTrap® RNA Cell HTS 96 Kit simplifies total RNA isolation from a variety of cell types.
The genomic DNA is removed without an enzymatic digestion step. RNases are inactivated to
prevent RNA degradation. The isolated RNA is ready to use for a broad panel of downstream
applications like RT-PCR, Northern Blotting and array technologies.
PURE RNA WITHOUT DNASE DIGESTION
Selective genomic DNA removal during lysis step
CLEANER RNA FOR BETTER RT-PCR RESULTS
No DNA contamination - up to 20 µg RNA in 30 minutes for 2 x 96 samples
OPTIMIZED PROTOCOL
For up to 5 x 105 human or animal cultured cells
Fig. 11: CROSS CONTAMINATION
Fig. 12: HIGH RNA PURITY - EFFECTIVE DNA REMOVAL
28S rRNA 18S rRNA -
RT
no RT
28S rRNA 18S rRNA -
_
+
_
+
_
+
_
+
Total RNA was isolated from 5 x 105 NIH 3T3 cells
using the InviTrap® RNA Cell HTS 96 Kit in a centrifuge. The samples and control samples without
template were arranged in alternating wells. 10 µl of
the eluted RNA were analyzed on a 1.2 % denaturing
agarose gel stained with ethidium bromide.
(+) Samples
(-) No Template Controls
PRODUCT
InviTrap® RNA Cell HTS 96 Kit/ C
Total RNA was prepared from 500, 3000 and 7000 3Y1 cells
according to the protocol for the InviTrap® RNA Cell HTS 96 Kit on
a centrifuge without DNase I treatment. 100 ng RNA were used
in real-time RT-PCR and standard PCR of the caveolin gene using
TaqMan technology to determine the amount of residual genomic
DNA and of amplifiable, intact RNA. The figures show the
RT-reaction in comparison with the no-RT-reaction.
Data kindly provided by Dr. A. Klippel, Silence Therapeutics AG,
Berlin, Germany.
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7061300200
24 x 96 purifications
7061300400
4 x 96 purifications
For RNA purification from tissue the InviTrap® RNA Tissue HTS 96 Kit (7062300200) is also available.
7061300300
04
14
DNA Fragment Purification
The fastest DNA fragment preparation
The MSB® HTS PCRapace is the fastest system for DNA fragment purification in 96 well format.
The kit uses a two-step procedure with only a binding and an elution step. Washing and drying
steps are not necessary. Use the MSB® HTS PCRapace for DNA product cleanup from amplification reaction mixtures, restriction digestion mixtures, ligation mixtures, cDNA synthesis
reactions, and DNA concentration.
READY IN 15 MINUTES USING A CENTRIFUGE
Remove primers, dNTPs, additives and salts without washing steps for 2 x 96 samples
HIGH RECOVERIES
95 % recovery of PCR products for fragment sizes from 80 bp – 30 kb
ENVIRONMENT- FRIENDLY
Less plastic waste and less power consumption due to reduced pipetting and centrifugation steps – only “bind-and-elute”
Fig. 13: RECOVERY ANALYSIS WITH AGILENT BIOANALYZER 2100
FRAGMENT SIZE
05
a
a
b
a
b
b
a
987 bp
b
a
b
a
b
1964 bp
BEFORE
PURIFICATION
AFTER
PURIFICATION
RECOVERY
RATE %
98 bp
14.59
11.86
81.32
538 bp
12.46
10.56
84.75
987 bp
27.32
26.23
96.02
1469 bp
45.76
36.78
80.38
538 bp
A
Randomly selected samples were analysed with the Bioanalyzer 2100 „Lab on a Chip“ system from Agilent. The figure shows the electropherogram for the fragments 538 bp, 987 bp and 1469 bp PCR products before (a) and after (b) purification using the MSB® HTS PCRapace
in duplicates. The table shows the recovery rates for the corresponding DNA fragment sizes in %.
PRODUCT
MSB® HTS PCRapace/ C
PACKAGE SIZE
CATALOGUE NUMBER
2 x 96 purifications
7021320200
24 x 96 purifications
7021320400
4 x 96 purifications
Please find ordering information for vacuum based or automated kits on page 16.
7021320300
15
KIT OVERVIEW
NUCLEID ACID
TYPE
ORIGIN
PRODUCTS
DNA
DNA fragments
DNA fragments, PCR products
MSB® HTS PCRapace/ C
MSB® HTS PCRapace/ V
14
DNA
plasmid
bacteria suspension
Invisorb® Plasmid HTS 96 Kit/ C
Invisorb® Plasmid HTS 96 Kit/ R
*
DNA
genomic
human & animal blood, bone marrow
Invisorb® Blood Mini HTS 96 Kit/ C
Invisorb® Blood Mini HTS 96 Kit/ X
Invisorb® Blood Mini HTS 96 Kit/ ep ***
Invisorb® Blood Mini HTS 96 Kit/ STARlet
InviMag® Blood Mini Kit/ STARlet
Invisorb® Universal HTS 96 Kit/ STARlet
InviMag® Universal Kit/ STARlet
05
(PCR products, ligation reaction mixture,
enzyme digestion mixture, cDNA synthesis
mixture)
(fresh, old, frozen whole blood with common
anticoagulants (EDTA or citrate, no heparin),
buffy coat)
PAGE
**
**
**
**
human & animal tissue, rodent
tails, formalin fixed paraffin embedded
tissue or insects
Invisorb® DNA Tissue HTS 96 Kit/ C
Invisorb® DNA Tissue HTS 96 Kit/ Tec
07
human & animal cells, cell cultures
Invisorb® DNA Tissue HTS 96 Kit/ C
Invisorb® DNA Tissue HTS 96 Kit/ Tec
07
bacteria species
Invisorb® Universal Bacteria HTS 96 Kit/ C
Invisorb® Universal Bacteria HTS 96 Kit/ V
Invisorb® Universal Bacteria HTS 96 Kit/ X
Invisorb® Universal Bacteria HTS 96 Kit/ ep ***
Invisorb® Universal HTS 96 Kit/ STARlet
InviMag® Universal Kit/ STARlet
11
(fresh and frozen tissue)
(from different kinds of starting materials,
bacteria pellets, tissue samples, blood, cellfree body fluids, urine, food samples, swabs)
forensic material
Invisorb® Forensic HTS 96 Kit/ C
saliva & swab material
PSP® SalivaGene DNA HTS 96 Kit/ C
PSP® SalivaGene DNA HTS 96 Kit/ ep
08
plant material
Invisorb® DNA Plant HTS 96 Kit/ C
12
clinical samples
Invisorb® Virus DNA HTS 96 Kit/ C
Invisorb® Virus DNA HTS 96 Kit/ V
Invisorb® Virus DNA HTS 96 Kit/ X
Invisorb® Virus DNA HTS 96 Kit/ ep ***
Invisorb® Universal HTS 96 Kit/ STARlet
InviMag® Universal Kit/ STARlet
*
plant material
Invisorb® DNA Plant HTS 96 Kit/ C
12
(clotted blood, bones, teeth, ancient bones,
nail clippings, hair roots, blood, saliva and
sperm stains, chewing gum, cigarette butts,
stamps and envelopes, tissue, swab material)
(fresh, frozen or dried plant tissue, such as
leaves, roots, fruits , seeds, water plants, GMO
– also for phytopathogens)
DNA
viral
(fresh or frozen plasma, serum, whole blood,
cell-free body fluids of human origin, rinse
liquid from swabs)
(fresh, frozen or dried plant tissue, such as
leaves, roots, fruits or seeds)
RNA
total
human & animal cells, cell cultures
InviTrap® RNA Cell HTS 96 Kit/ C
13
InviTrap® RNA Tissue HTS 96 Kit/ C
12
plant material
InviTrap® RNA Plant HTS 96 Kit/ C
12
clinical samples
Invisorb® Virus RNA HTS 96 Kit/ C
Invisorb® Virus RNA HTS 96 Kit/ V
Invisorb® Virus RNA HTS 96 Kit/ X
Invisorb® Virus RNA HTS 96 Kit/ ep ***
Invisorb® Virus RNA HTS 96 Kit/ STARlet
InviMag® Virus RNA Kit/ STARlet
Invisorb® Universal HTS 96 Kit/ STARlet
InviMag® Universal Kit/ STARlet
10
**
**
**
**
InviTrap® RNA Plant HTS 96 Kit/ C
12
(fresh or frozen plant tissue and filamenteous fungi)
viral
**
**
human & animal tissue
(fresh and frozen tissue)
RNA
**
**
*
(cell-free body fluids of human origin, rinse
liquid or transport media from swabs, cell
culture supernatants, stool samples)
plant material
(fresh or frozen plant tissue and filamenteous fungi )
The Invisorb® and InviTrap® HTS kits in 96 well format can be used in a centrifuge, on a
vacuum manifold or on robotic workstations. The InviMag® HTS kits in 96 well format can
only be used on robotic platforms. For adaption to robotic platforms please contact us
([email protected]) or your local sales representative.
*) Please find more information online at www.invitek.de
**) Please request specific brochure about Kits on STARlet from Hamilton Robotics
***) On request only
16
ORDERING INFORMATION
PRODUCT
PACKAGE SIZE
CATALOG NUMBER
Invisorb® Blood Mini HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7031300200
7031300300
7031300400
Invisorb® Blood Mini HTS 96 Kit/ X
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7131310200
7131310300
7131310400
InviMag® Universal Kit/ STARlet
4 x 96 purifications
24 x 96 purifications
7450330300
7450330400
Invisorb® DNA Tissue HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7032900200
7032900300
7032900400
PSP® SalivaGene DNA HTS 96 Kit / C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7035360200
7035360300
7035360400
SalivaGene® Collector
50 pieces
1035210200
SalivaGene® Buccal Swab
50 pieces
1035230200
Invisorb® Virus RNA HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7043300200
7043300300
7043300400
Invisorb® Virus RNA HTS 96 Kit/ V
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7043310200
7043310300
7043310400
Invisorb® Virus RNA HTS 96 Kit/ X
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7143310200
7143310300
7143310400
Invisorb® Virus DNA HTS 96 Kit/ C
(using a centrifuge)
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7042300200
7042300300
7042300400
InviMag® Universal Kit/ STARlet
4 x 96 purifications
24 x 96 purifications
7450330300
7450330400
Invisorb® Universal Bacteria HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7033300200
7033300300
7033300400
Invisorb® Universal Bacteria HTS 96 Kit/ X
(for use on X-tractor Gene)
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7133310200
7133310300
7133310400
InviMag® Universal Kit/ STARlet
4 x 96 purifications
24 x 96 purifications
7450330300
7450330400
Invisorb® DNA Plant HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7037300200
7037300300
7037300400
InviTrap® RNA Plant HTS 96 Kit/ C
4 x 96 purifications
24 x 96 purifications
7064300300
7064300400
InviTrap® RNA Cell HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7061300200
7061300300
7061300400
InviTrap® RNA Tissue HTS 96 Kit/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7062300200
7062300300
7062300400
InviTrap® RNA Plant HTS 96 Kit/ C
(using a centrifuge)
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7062300200
7062300300
7062300400
MSB® HTS PCRapace/ C
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7021320200
7021320300
7021320400
Invisorb® Plasmid HTS 96 Kit/ C
(using a centrifuge)
2 x 96 purifications
4 x 96 purifications
24 x 96 purifications
7010300200
7010300300
7010300400
(using a centrifuge)
(for use on X-tractor Gene)
(using a centrifuge)
(using a centrifuge)
(using a centrifuge)
(using a vacuum manifold)
(for use on X-tractor Gene)
(using a centrifuge)
(using a centrifuge)
(using a centrifuge)
(using a centrifuge)
(using a centrifuge)
(using a centrifuge)
STRATEC Molecular GmbH
Robert-Rössle-Str. 10
13125 Berlin, Germany
Phone: +49 30 94 89 29 01 / 29 10
Fax: +49 30 94 89 29 09
E-mail: [email protected]
www.invitek.de
www.stratec.com
Invisorb®, InviTrap®, ®, InviMag®,
MSB®, PSP® and SalivaGene® are
registered trademarks of STRATEC
Molecular.
Other brands or product names
are trademarks of their respective
holders.
© 2013 STRATEC Molecular,
all rights reserved.
6C7o/06/2013
YOUR LOCAL DISTRIBUTOR:
STRATEC Molecular GmbH
Robert-Rössle-Str. 10
13125 Berlin, Germany
Phone: +49 30 94 89 29 01
Fax: +49 30 94 89 29 09
E-mail: [email protected]
www.invitek.de