Identification and first characterization of pairing
... are accidentally transported via the blood stream to inner organs such as the liver and spleen, where they cause additional inflammatory processes, resulting in granuloma formation and finally leading to hepatosplenomegaly and cirrhosis (Ross et al., 2012). Successful tissue passage of eggs takes ap ...
... are accidentally transported via the blood stream to inner organs such as the liver and spleen, where they cause additional inflammatory processes, resulting in granuloma formation and finally leading to hepatosplenomegaly and cirrhosis (Ross et al., 2012). Successful tissue passage of eggs takes ap ...
Understanding the Regulation of Metabolic Enzyme Acetylation in E
... Although much is known about the affects and regulation of CheY, Acs, RcsB, the α subunit of RNAP, and RNase R acetylation, there are still more than 245 proteins in E. coli reported to be acetylated, whose regulation and effects of protein acetylation remain unknown. Interestingly, of all the acety ...
... Although much is known about the affects and regulation of CheY, Acs, RcsB, the α subunit of RNAP, and RNase R acetylation, there are still more than 245 proteins in E. coli reported to be acetylated, whose regulation and effects of protein acetylation remain unknown. Interestingly, of all the acety ...
Identification and characterization of novel interaction
... C-terminal domain of the α subunit of the RNA polymerase C-terminal domain of the α subunit of the RNA polymerase Adenosindiphosphate Ampicillin Adenosinmomophosphate Ammoniumperoxodisulfate Adenosintriphosphate Bacillus base pair bovine serum albumin casamino acids cyclic Adenosinmomophosphate Cata ...
... C-terminal domain of the α subunit of the RNA polymerase C-terminal domain of the α subunit of the RNA polymerase Adenosindiphosphate Ampicillin Adenosinmomophosphate Ammoniumperoxodisulfate Adenosintriphosphate Bacillus base pair bovine serum albumin casamino acids cyclic Adenosinmomophosphate Cata ...
One-stop polymerase chain reaction (PCR): An improved PCR
... Figure 2. Efficiency and effect of one-stop PCR on nucleic acid amplification. (A) One-stop PCR is comparable to commercially available PCR kits. A 0.4 kb fragment of human β-actin mRNA was amplified with gradient template amount at dilutions of 1/1, 1/10, 1/100. All the PCR products were separated ...
... Figure 2. Efficiency and effect of one-stop PCR on nucleic acid amplification. (A) One-stop PCR is comparable to commercially available PCR kits. A 0.4 kb fragment of human β-actin mRNA was amplified with gradient template amount at dilutions of 1/1, 1/10, 1/100. All the PCR products were separated ...
Origin of the catalytic activity of bovine seminal ribonuclease against
... RNase homologue. In BS RNase, Lys residues replace Gln55, Asn-62, Tyr-76, and Asn-113 in pancreatic RNase and Arg replaces Ser-80. The pattern of glycosylation of ribonucleases is also believed to influence catalytic activity against duplex RNA (36, 38-40). We report here a systematic study of the r ...
... RNase homologue. In BS RNase, Lys residues replace Gln55, Asn-62, Tyr-76, and Asn-113 in pancreatic RNase and Arg replaces Ser-80. The pattern of glycosylation of ribonucleases is also believed to influence catalytic activity against duplex RNA (36, 38-40). We report here a systematic study of the r ...
- VU Research Repository
... been discovered that yeast acclimatise more quickly to ethanol stress in the presence of low acetaldehyde concentrations; however, the biochemical processes underpinning this effect are unknown. The objective of this project was to identify the mechanisms associated with the acetaldehyde-mediated ad ...
... been discovered that yeast acclimatise more quickly to ethanol stress in the presence of low acetaldehyde concentrations; however, the biochemical processes underpinning this effect are unknown. The objective of this project was to identify the mechanisms associated with the acetaldehyde-mediated ad ...
Sequence variation characteristics of D
... (Gadaleta et al., 1989), chicken {Desjiardins and Morals, 1990), fin whale (Arnason et al., 1991), and the blue moilusk My/ilii.s eJiili.s (Hoffman el ai, 1992). The mitochondrial genome is much larger in yeast. The exact size varies quite widely among different strains of Sacchromyces cerevisiae, i ...
... (Gadaleta et al., 1989), chicken {Desjiardins and Morals, 1990), fin whale (Arnason et al., 1991), and the blue moilusk My/ilii.s eJiili.s (Hoffman el ai, 1992). The mitochondrial genome is much larger in yeast. The exact size varies quite widely among different strains of Sacchromyces cerevisiae, i ...
Structure and Function of Mammalian Ribosomes
... and function in mammalian ribosomes. Our first goal was to isolate structurally and functionally intact ribosomal subunits. Preparation of bacterial subunits with these qualities hasbeen possiblefor many years (Gilbert, 1963a; Gesteland, 1966). However, until recently the same accomplishment with ma ...
... and function in mammalian ribosomes. Our first goal was to isolate structurally and functionally intact ribosomal subunits. Preparation of bacterial subunits with these qualities hasbeen possiblefor many years (Gilbert, 1963a; Gesteland, 1966). However, until recently the same accomplishment with ma ...
+ΔFosB
... - Nave is the mean N value for the control - Ctave is the mean Ct value for the control. - Fold differences (stress ChIP relative to control ChIP) were then determined by raising 2 to the Ct power. Input : One hundred microliters of the pre-immunoprecipitated lysate Control ChIP : samples were immun ...
... - Nave is the mean N value for the control - Ctave is the mean Ct value for the control. - Fold differences (stress ChIP relative to control ChIP) were then determined by raising 2 to the Ct power. Input : One hundred microliters of the pre-immunoprecipitated lysate Control ChIP : samples were immun ...
Metabolic decision making by protein-metabolite - ETH E
... Metabolism lies at the core of microbial life and fuels all cellular activities with building blocks, reducing power, and energy. To regulate metabolic activity, microbes utilize a complex network of regulatory layers, such as transcriptional regulation, covalent posttranslational modificat ...
... Metabolism lies at the core of microbial life and fuels all cellular activities with building blocks, reducing power, and energy. To regulate metabolic activity, microbes utilize a complex network of regulatory layers, such as transcriptional regulation, covalent posttranslational modificat ...
Locked Nucleic Acid (LNA™)
... the oligonucleotide and great care must be taken to find the right design for your experimental purpose. While you will want to take advantage of the properties of LNA™ in order to achieve high target specificity it is important not to use too much LNA™ since this can result in a very “sticky” oligo ...
... the oligonucleotide and great care must be taken to find the right design for your experimental purpose. While you will want to take advantage of the properties of LNA™ in order to achieve high target specificity it is important not to use too much LNA™ since this can result in a very “sticky” oligo ...
Origins and Early Evolution of the tRNA Molecule
... Abstract: Modern transfer RNAs (tRNAs) are composed of ~76 nucleotides and play an important role as “adaptor” molecules that mediate the translation of information from messenger RNAs (mRNAs). Many studies suggest that the contemporary full-length tRNA was formed by the ligation of half-sized hairp ...
... Abstract: Modern transfer RNAs (tRNAs) are composed of ~76 nucleotides and play an important role as “adaptor” molecules that mediate the translation of information from messenger RNAs (mRNAs). Many studies suggest that the contemporary full-length tRNA was formed by the ligation of half-sized hairp ...
In situ hybridization
... hybridizing the complementary strand of a nucleotide probe to the sequence of interest. Normal hybridization requires the isolation of DNA or RNA, separating it on a gel, blotting it onto nitrocellulose and probing it with a complementary sequence. The basic principles for in situ hybridization are ...
... hybridizing the complementary strand of a nucleotide probe to the sequence of interest. Normal hybridization requires the isolation of DNA or RNA, separating it on a gel, blotting it onto nitrocellulose and probing it with a complementary sequence. The basic principles for in situ hybridization are ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... in Escherichia coli and the physical and regulatory properties of the operon’s controlling gene, araC. It also describes the light switch mechanism as an explanation for many of the protein’s properties. Although many thousands of homologs of AraC exist and regulate many diverse operons in response ...
... in Escherichia coli and the physical and regulatory properties of the operon’s controlling gene, araC. It also describes the light switch mechanism as an explanation for many of the protein’s properties. Although many thousands of homologs of AraC exist and regulate many diverse operons in response ...
RNA interference - Bio
... Choose target sites from among the sequences identified in Step 1 based on the guidelines: ...
... Choose target sites from among the sequences identified in Step 1 based on the guidelines: ...
Investigating the link between tRNA and mRNA - EMBL-EBI
... This thesis summarises my research into the control of the abundance of trnas by individual trna gene expression changes in mammalian organisms. I will show that trna gene expression is subject to tight regulation, and that the abundance of trna molecules is thus kept highly stable even across vastl ...
... This thesis summarises my research into the control of the abundance of trnas by individual trna gene expression changes in mammalian organisms. I will show that trna gene expression is subject to tight regulation, and that the abundance of trna molecules is thus kept highly stable even across vastl ...
Cells: building blocks of living organisms
... Organelles are discrete structures having specialized functions ...
... Organelles are discrete structures having specialized functions ...
attachment of amino acids to tRNA
... small and large ribosome subunits associate with each other and the mRNA, translate it, and then dissociate after each round of translation. This sequence of association and dissociation is called the ribosome cycle. ...
... small and large ribosome subunits associate with each other and the mRNA, translate it, and then dissociate after each round of translation. This sequence of association and dissociation is called the ribosome cycle. ...
Identity elements in tRNA-mediated transcription
... These results showed that when acceptor and antiacceptor are compatible, specifier codon–anticodon complementarity is neither sufficient for an efficient response to occur nor strictly indispensable for a low level response, and consequently suggested the existence of additional means of recognition ...
... These results showed that when acceptor and antiacceptor are compatible, specifier codon–anticodon complementarity is neither sufficient for an efficient response to occur nor strictly indispensable for a low level response, and consequently suggested the existence of additional means of recognition ...
The deleterious effect of missense mutations on pre
... Whether a suspected disease-causing mutation affects splicing should ideally be determined in RNA from the affected tissue because cis-acting splicing mutations can have tissue-specific effects. Unfortunately, the appropriate tissues are often not available. Frequently, the study of RNA extracted fr ...
... Whether a suspected disease-causing mutation affects splicing should ideally be determined in RNA from the affected tissue because cis-acting splicing mutations can have tissue-specific effects. Unfortunately, the appropriate tissues are often not available. Frequently, the study of RNA extracted fr ...
Comparison of conserved structural and regulatory domains within
... structure models were then made with RNAdraw for each sequence. These models enabled us to validate the alignment of conserved domains such as D1, D1h, D2, D3, D4 and box A, and to deduce their roles in the formation of the structure of the transcript or in the control of transcription (see below). ...
... structure models were then made with RNAdraw for each sequence. These models enabled us to validate the alignment of conserved domains such as D1, D1h, D2, D3, D4 and box A, and to deduce their roles in the formation of the structure of the transcript or in the control of transcription (see below). ...
Practical Aspects of Estimating Energy Components in
... High expression of SAA in carcinomas was detected mostly in tumor cells, but not in normal mucosa. AG490, inhibitor of STAT3 activation, reduced SAA1 expression in SAS cells. SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway ...
... High expression of SAA in carcinomas was detected mostly in tumor cells, but not in normal mucosa. AG490, inhibitor of STAT3 activation, reduced SAA1 expression in SAS cells. SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway ...
Tricarboxylic Acid Cycle-Dependent Regulation of Staphylococcus
... complementation plasmid. The pMRS2 complementation plasmid was constructed by amplifying the 2.7-kb promoterless acnA gene from S. epidermidis strain 1457 using SalI-SD-acnA-f and SacI-acnA-r primers, followed by ligation of the PCR product into plasmid pCL15 digested with SalI and SacI. Plasmid pMR ...
... complementation plasmid. The pMRS2 complementation plasmid was constructed by amplifying the 2.7-kb promoterless acnA gene from S. epidermidis strain 1457 using SalI-SD-acnA-f and SacI-acnA-r primers, followed by ligation of the PCR product into plasmid pCL15 digested with SalI and SacI. Plasmid pMR ...
Chromatin Condensing Functions of the Linker Histone C
... order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered protein domains in eukaryotic proteomes (23), there is a relative paucity of experimental biochemical data that address the ...
... order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered protein domains in eukaryotic proteomes (23), there is a relative paucity of experimental biochemical data that address the ...
Eukaryotic transcription
Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Unlike prokaryotic RNA polymerase that initiates the transcription of all different types of RNA, RNA polymerase in eukaryotes (including humans) comes in three variations, each encoding a different type of gene. A eukaryotic cell has a nucleus that separates the processes of transcription and translation. Eukaryotic transcription occurs within the nucleus where DNA is packaged into nucleosomes and higher order chromatin structures. The complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.