Supplementary Text S1: Detailed PCR Protocols
... SYBR green readout and the primer IFNB1 OF that only uses the control oligonucleotides, present at 200 copies, as its template (Supplementary Figure S5B). This control assay demonstrated a very tight distribution in the presence of the genomic standard dilution series, suggesting PCR efficiency was ...
... SYBR green readout and the primer IFNB1 OF that only uses the control oligonucleotides, present at 200 copies, as its template (Supplementary Figure S5B). This control assay demonstrated a very tight distribution in the presence of the genomic standard dilution series, suggesting PCR efficiency was ...
Molecular insights into RNA and DNA helicase evolution from the
... RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a three-dimensional structure that is held in shape by regions of the molecule interacting with each other. Before DNA and RNA can perform their essential tasks in cells, enzymes called helicases must s ...
... RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a three-dimensional structure that is held in shape by regions of the molecule interacting with each other. Before DNA and RNA can perform their essential tasks in cells, enzymes called helicases must s ...
High throughput nucleic acid sample preparation in 96 well plates
... The Invisorb® DNA Tissue HTS 96 Kit enables genomic DNA purification from a variety of tissue types incl. mouse and rat tails, cryosections, food and from eucaryotic cells. PCR inhibitors, which can interfere with the amplification reaction, are efficiently removed. Pure DNA can be used in subsequen ...
... The Invisorb® DNA Tissue HTS 96 Kit enables genomic DNA purification from a variety of tissue types incl. mouse and rat tails, cryosections, food and from eucaryotic cells. PCR inhibitors, which can interfere with the amplification reaction, are efficiently removed. Pure DNA can be used in subsequen ...
Mechanisms of Unidirectional Translocation & Unwinding
... Stepping requires at least 2 NA binding sites that independently bind and release NA and change the distance between each other. • Monomeric helicases: 2 hands represent 2 parts of NA binding site that move relative to each other. Both sites are controlled by a single NTPase site. • Oligomeric helic ...
... Stepping requires at least 2 NA binding sites that independently bind and release NA and change the distance between each other. • Monomeric helicases: 2 hands represent 2 parts of NA binding site that move relative to each other. Both sites are controlled by a single NTPase site. • Oligomeric helic ...
Restriction fragment differential display of pediocin
... fragments. The analysis was therefore carried out with primers degenerate in the third position, NNA\G or NNC\T, giving a 50 % workload reduction. The amplifications contained on average 50–70 fragments (between 10 and 100 fragments), the majority of which were under 400 bp, the largest being 770 bp ...
... fragments. The analysis was therefore carried out with primers degenerate in the third position, NNA\G or NNC\T, giving a 50 % workload reduction. The amplifications contained on average 50–70 fragments (between 10 and 100 fragments), the majority of which were under 400 bp, the largest being 770 bp ...
TRIzol Reagent
... TRIzol LS is a complete, ready-to use reagent for easy and simultaneous isolation of total RNA, DNA and proteins from liquid samples. The reagent, a mono-phasic solution of phenol and guanidinium thiocyanate, is an improvement to the single step RNA isolation method developed by Chomzcynski and Sacc ...
... TRIzol LS is a complete, ready-to use reagent for easy and simultaneous isolation of total RNA, DNA and proteins from liquid samples. The reagent, a mono-phasic solution of phenol and guanidinium thiocyanate, is an improvement to the single step RNA isolation method developed by Chomzcynski and Sacc ...
Synthesis of Oligonucleotides
... to protect guanine (Figure 4.2). Thymidine does not require protection since it does not have an exocyclic amino group. While these acyl protecting groups are still suitable for oligonucleotide synthesis today, new chemistries and new nucleoside building blocks have been introduced, which require mi ...
... to protect guanine (Figure 4.2). Thymidine does not require protection since it does not have an exocyclic amino group. While these acyl protecting groups are still suitable for oligonucleotide synthesis today, new chemistries and new nucleoside building blocks have been introduced, which require mi ...
Alu Background Information
... DNA loci. Alu elements can be sorted into distinct lineages, or families, according to inherited patterns of new mutations. These studies suggest that the rate of Alu transposition has changed over time – from about one new jump in every live birth, early in primate evolution, to about one in every ...
... DNA loci. Alu elements can be sorted into distinct lineages, or families, according to inherited patterns of new mutations. These studies suggest that the rate of Alu transposition has changed over time – from about one new jump in every live birth, early in primate evolution, to about one in every ...
The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA
... Figure 8. Results from the Qubit RNA HS assay for samples containing RNA plus 25 ng/μL glycogen or GlycoBlue Coprecipitant (in the assay tube) compared to samples containing RNA only. The RNA concentration in the assay tubes was 500 ng/mL (near the high end of the Qubit RNA HS assay range). Note tha ...
... Figure 8. Results from the Qubit RNA HS assay for samples containing RNA plus 25 ng/μL glycogen or GlycoBlue Coprecipitant (in the assay tube) compared to samples containing RNA only. The RNA concentration in the assay tubes was 500 ng/mL (near the high end of the Qubit RNA HS assay range). Note tha ...
REPLI-g WTA Single Cell Handbook
... All enzymatic steps have been specifically developed to enable efficient processing of RNA for accurate amplification. For example, these processes include effective gDNA removal prior to cDNA synthesis. Novel REPLI-g SensiPhi DNA Polymerase is used for Multiple Displacement Amplification (MDA). ...
... All enzymatic steps have been specifically developed to enable efficient processing of RNA for accurate amplification. For example, these processes include effective gDNA removal prior to cDNA synthesis. Novel REPLI-g SensiPhi DNA Polymerase is used for Multiple Displacement Amplification (MDA). ...
Gene Silencing without DNA: RNA-Mediated Cross
... 1997; Ratcliff et al., 1997) that do not have homology to endogenous genes. In these examples, the infected plants exhibit a response very similar to the virus-induced recovery on transgenic plants in that the upper leaves are symptom free and contain reduced levels of virus. In nepovirus-infected p ...
... 1997; Ratcliff et al., 1997) that do not have homology to endogenous genes. In these examples, the infected plants exhibit a response very similar to the virus-induced recovery on transgenic plants in that the upper leaves are symptom free and contain reduced levels of virus. In nepovirus-infected p ...
Isr J Chem (2010) - Weizmann Institute of Science
... formation. We aim to define the minimal ribosomal component required for maintaining PTC structure and function, in order to shed light on the evolution of the ribosome. Since the discovery made in the 1960 s that the ribosome can catalyze peptide bond formation between minimal substrates, e. g. pur ...
... formation. We aim to define the minimal ribosomal component required for maintaining PTC structure and function, in order to shed light on the evolution of the ribosome. Since the discovery made in the 1960 s that the ribosome can catalyze peptide bond formation between minimal substrates, e. g. pur ...
a server for analyzing and predicting RNA
... calculated RNA-binding residues are also displayed on the PDB structure of the protein–RNA complex using Jmol (www.jmol.org). By default, the RNA-binding residues are displayed in red space-fill representation, the rest of the protein is displayed in blue space-fill and the bound RNA is displayed in g ...
... calculated RNA-binding residues are also displayed on the PDB structure of the protein–RNA complex using Jmol (www.jmol.org). By default, the RNA-binding residues are displayed in red space-fill representation, the rest of the protein is displayed in blue space-fill and the bound RNA is displayed in g ...
The Genetic Code and RNA-Amino Acid Affinities
... contiguous sequences, usually having ≤24 essential nucleotides, should be the most likely to be isolated. These 24 “essential nucleotides” are defined by statistics. If only purines occur at a given position, this is twice as likely to occur as one specific nucleotide. In this case, selection can is ...
... contiguous sequences, usually having ≤24 essential nucleotides, should be the most likely to be isolated. These 24 “essential nucleotides” are defined by statistics. If only purines occur at a given position, this is twice as likely to occur as one specific nucleotide. In this case, selection can is ...
Amiito acid sequence of the testosterone
... daltons. This agrees with the previous sizings of the in vitro synthesized polypeptide as 42,000 daltons (2) and 43,000 daltons (1). The amino acid sequence is rich in proline residues (9.8%) which undoubtably have a significant effect on the secondary structure of the protein by introducing bends a ...
... daltons. This agrees with the previous sizings of the in vitro synthesized polypeptide as 42,000 daltons (2) and 43,000 daltons (1). The amino acid sequence is rich in proline residues (9.8%) which undoubtably have a significant effect on the secondary structure of the protein by introducing bends a ...
Chpt14_Translation.doc
... ribosome ready for the elongation phase of translation. Note that f-mettRNAfmet is positioned at the P site. It has recognized the initiator AUG in the mRNA. 4. Identification of initiator AUG in eukaryotes a. Bases around AUG influence efficiency of initiation. (1) The most important effects are fr ...
... ribosome ready for the elongation phase of translation. Note that f-mettRNAfmet is positioned at the P site. It has recognized the initiator AUG in the mRNA. 4. Identification of initiator AUG in eukaryotes a. Bases around AUG influence efficiency of initiation. (1) The most important effects are fr ...
The polymerase chain reaction
... • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequ ...
... • Thermal asymmetric interlaced PCR (TAIL-PCR): for isolation of an unknown sequence flanking a known sequence. Within the known sequence, TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequ ...
Reverse Transcription PCR (RT-PCR): The Molecular
... or LTRs (see Figure 2). The integrated DNA is called proviral DNA or the provirus. The provirus enters a period of latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the majo ...
... or LTRs (see Figure 2). The integrated DNA is called proviral DNA or the provirus. The provirus enters a period of latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the majo ...
Friesland Foods Normal
... organisation, the identity of the EC numbers and the position in the reaction network (see Figure 1). Small differences in operon organization between the families Lactobacillaceae, Enterococcaceae and Leuconostocaceae (Table 1) and the family Streptococcaceae (Table 2) were observed, implying a dif ...
... organisation, the identity of the EC numbers and the position in the reaction network (see Figure 1). Small differences in operon organization between the families Lactobacillaceae, Enterococcaceae and Leuconostocaceae (Table 1) and the family Streptococcaceae (Table 2) were observed, implying a dif ...
Translation tRNA is a link between the mRNA and the polypeptide
... RF-1 which recognizes the termination codons UAA and UAG RF-2 which recognizes UAA and UGA RF-3 which plays supporting role. ...
... RF-1 which recognizes the termination codons UAA and UAG RF-2 which recognizes UAA and UGA RF-3 which plays supporting role. ...
Microenvironment analysis and identification of magnesium binding
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
Press Release
... Now a team from EMBL Heidelberg and Stanford University led by Lars Steinmetz has turned this on its head. The researchers have shown that one end of the mRNA begins to decay while the other is still serving as a template for protein production. Thus, studying the decaying mRNA also provides a snaps ...
... Now a team from EMBL Heidelberg and Stanford University led by Lars Steinmetz has turned this on its head. The researchers have shown that one end of the mRNA begins to decay while the other is still serving as a template for protein production. Thus, studying the decaying mRNA also provides a snaps ...
RNA–Amino Acid Binding - University of Colorado-MCDB
... side chain is hooked, not quite completely extended in fitting its site (Fig. 4). Further, the site has been shown to tolerate variation and bulky, even polar, substitutions midside chain, as long as the side chain remains about the right length (Blount et al. 2007). This observed tolerance for modi ...
... side chain is hooked, not quite completely extended in fitting its site (Fig. 4). Further, the site has been shown to tolerate variation and bulky, even polar, substitutions midside chain, as long as the side chain remains about the right length (Blount et al. 2007). This observed tolerance for modi ...
Gene Therapy and Transgenic Animals
... of sequence the minimum ribozyme structure into and plasmids introduction 1. Identification Ribozyme-coding has been incorporated and of chemical modifications that retain ribozyme activity and administered, in effect ribozyme gene therapy. enhancing stability to nucleases. ...
... of sequence the minimum ribozyme structure into and plasmids introduction 1. Identification Ribozyme-coding has been incorporated and of chemical modifications that retain ribozyme activity and administered, in effect ribozyme gene therapy. enhancing stability to nucleases. ...
structure and mechanism of dna polymerases
... function in a distributive manner; they do not remain bound to the DNA during multiple cycles of nucleotide addition, so as not to cause mutagenic incorporation events after lesion bypass is completed. The distributive mode of synthesis should also allow their displacement by the more processive rep ...
... function in a distributive manner; they do not remain bound to the DNA during multiple cycles of nucleotide addition, so as not to cause mutagenic incorporation events after lesion bypass is completed. The distributive mode of synthesis should also allow their displacement by the more processive rep ...
Eukaryotic transcription
Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Unlike prokaryotic RNA polymerase that initiates the transcription of all different types of RNA, RNA polymerase in eukaryotes (including humans) comes in three variations, each encoding a different type of gene. A eukaryotic cell has a nucleus that separates the processes of transcription and translation. Eukaryotic transcription occurs within the nucleus where DNA is packaged into nucleosomes and higher order chromatin structures. The complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.