The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
... D. Kinetics of removal of VPg from poliovirus RNA in Hela cell extracts........................................ 84 E. Release of VPg from polio RNA in other cell extracts..... 88 F. Glycerol gradient sedimentation of Hela unlinking activity.................................................. 91 G. Mag ...
SURVEY AND SUMMARY A profusion of upstream open reading
... Particular nascent peptide sequences, while still within the ribosome, can lead to ribosome stalling. Upstream ORFs which exhibit such sequence dependent stalling are in contrast to those uORFs whose coding sequence identity is immaterial—the degree of influence of this category is wholly dependent o ...
... Particular nascent peptide sequences, while still within the ribosome, can lead to ribosome stalling. Upstream ORFs which exhibit such sequence dependent stalling are in contrast to those uORFs whose coding sequence identity is immaterial—the degree of influence of this category is wholly dependent o ...
SPT4, a gene important for tr
... Plasmids. The plasmids pJF17, pJF42, and pBM25 contain subclones of the cloned SPT4 gene in the centromere-containing vector YCp50 (Johnston and Davis 1984) and were used to delimit the SPT4 gene (Fig. 1). The HindIII fragment that contains SPT4 function was cloned in the integrating vector YIp5 (St ...
... Plasmids. The plasmids pJF17, pJF42, and pBM25 contain subclones of the cloned SPT4 gene in the centromere-containing vector YCp50 (Johnston and Davis 1984) and were used to delimit the SPT4 gene (Fig. 1). The HindIII fragment that contains SPT4 function was cloned in the integrating vector YIp5 (St ...
Polymerase Chain Reaction In Ophthalmology
... Relatively quick, as electrophoresis is not required. ...
... Relatively quick, as electrophoresis is not required. ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... to perform numerous tasks on complex macromolecular objects, namely cellular DNA. The DNA in living cells has a highly heterogeneous and dynamic structure upon which groups of DNA-processing proteins need to perform dedicated tasks. Some of these, such as the search for a damaged base, a promoter se ...
... to perform numerous tasks on complex macromolecular objects, namely cellular DNA. The DNA in living cells has a highly heterogeneous and dynamic structure upon which groups of DNA-processing proteins need to perform dedicated tasks. Some of these, such as the search for a damaged base, a promoter se ...
Class I tRNA
... RNA is concentrated at the interface with the 50S subunit. Both 30S and 50S subunits are self-assembled in vitro. In 30S subunit, S4 and S8 bind to 16S rRNA first, other proteins then join sequentially and cooperatively. ...
... RNA is concentrated at the interface with the 50S subunit. Both 30S and 50S subunits are self-assembled in vitro. In 30S subunit, S4 and S8 bind to 16S rRNA first, other proteins then join sequentially and cooperatively. ...
Document
... are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. ...
... are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. ...
USMLE Step 1 - Becker Professional Education
... of State Medical Boards (FSMB) and National Board of Medical Examiners® (NBME®). United States Medical Licensing Examination, USMLE, National Board of Medical Examiners, and NBME are registered trademarks of the National Board of Medical Examiners. The National Board of Medical Examiners does not sp ...
... of State Medical Boards (FSMB) and National Board of Medical Examiners® (NBME®). United States Medical Licensing Examination, USMLE, National Board of Medical Examiners, and NBME are registered trademarks of the National Board of Medical Examiners. The National Board of Medical Examiners does not sp ...
488KB - The Doudna Lab - University of California, Berkeley
... The structure of G. intestinalis Dicer revealed several patches of positively charged residues on the surface of the protein, particularly along the surface connecting the PAZ and the catalytic domains. To test the contributions of these residues to dsRNA substrate recognition, nine Dicer protein mu ...
... The structure of G. intestinalis Dicer revealed several patches of positively charged residues on the surface of the protein, particularly along the surface connecting the PAZ and the catalytic domains. To test the contributions of these residues to dsRNA substrate recognition, nine Dicer protein mu ...
Fulltext PDF
... language of nucleic acids, the specificity of charging the right amino acid on the cognate tRNA is 'bilingual', involving a protein and a nucleic acid. The major role played by aa-tRNA sythetase in maintaining the fidelity in charging of tRNA with the right amino acid was subsequently deciphered. It ...
... language of nucleic acids, the specificity of charging the right amino acid on the cognate tRNA is 'bilingual', involving a protein and a nucleic acid. The major role played by aa-tRNA sythetase in maintaining the fidelity in charging of tRNA with the right amino acid was subsequently deciphered. It ...
PDF File
... structure of the Tetrahymena intron (Kim and Cech, 1987); this model is shown in Figure 3. To guide the modeling, Kim and Cech used information about residue accessibility from chemical and enzymatic probes, a predilection to place the most conserved elements together in a catalytic core or center, ...
... structure of the Tetrahymena intron (Kim and Cech, 1987); this model is shown in Figure 3. To guide the modeling, Kim and Cech used information about residue accessibility from chemical and enzymatic probes, a predilection to place the most conserved elements together in a catalytic core or center, ...
Late events of translation initiation in bacteria: a kinetic analysis
... shown above, this step was fast compared with the subsequent reactions and was therefore not considered in the kinetic model (Materials and methods). The next step of GTP hydrolysis by IF2 proceeded with a rate constant of 30/s (cf. Figure 1) followed by Pi release, 1.5/s. When GDP or no nucleotide ...
... shown above, this step was fast compared with the subsequent reactions and was therefore not considered in the kinetic model (Materials and methods). The next step of GTP hydrolysis by IF2 proceeded with a rate constant of 30/s (cf. Figure 1) followed by Pi release, 1.5/s. When GDP or no nucleotide ...
Function and Control of the Spx-Family of Proteins Within
... sporulation in response to nutritional stress. This is a process of cellular differentiation in which a vegetative cell transitions into a prespore state in which the cell is composed of two compartments, one of which will become the dormant spore and the larger compartment, or mother cell that supp ...
... sporulation in response to nutritional stress. This is a process of cellular differentiation in which a vegetative cell transitions into a prespore state in which the cell is composed of two compartments, one of which will become the dormant spore and the larger compartment, or mother cell that supp ...
Pseudomon-1 motif
... decision in the present work to group bacteria at the level of order, rather than the more-broad phylum or class, also might help to reduce spurious sequences in clusters. 3. The use of environmental sequences helped to find RNAs that are not well represented in organisms whose genomes have been fu ...
... decision in the present work to group bacteria at the level of order, rather than the more-broad phylum or class, also might help to reduce spurious sequences in clusters. 3. The use of environmental sequences helped to find RNAs that are not well represented in organisms whose genomes have been fu ...
Next Generation Quantitative PCR
... allowing the user to easily incorporate a reliable positive control into ...
... allowing the user to easily incorporate a reliable positive control into ...
5 DNA Replication
... must start a new at the replication fork and proceed in the direction opposite that of the movement of the fork until it runs into the previously replicated segment of DNA. This process is repeated again and again, so synthesis of this strand is in short, discontinuous bursts. The newly made strand ...
... must start a new at the replication fork and proceed in the direction opposite that of the movement of the fork until it runs into the previously replicated segment of DNA. This process is repeated again and again, so synthesis of this strand is in short, discontinuous bursts. The newly made strand ...
PCR Cloning Considerations
... The use of fresh PCR products in TA, TOPO® TA, and Directional TOPO® Cloning is recommended due the potential presence of exonucleases that will, over time, degrade the nucleotide overhangs, reducing the efficiency of the cloning event. While it is not recommended, some PCR products have been succes ...
... The use of fresh PCR products in TA, TOPO® TA, and Directional TOPO® Cloning is recommended due the potential presence of exonucleases that will, over time, degrade the nucleotide overhangs, reducing the efficiency of the cloning event. While it is not recommended, some PCR products have been succes ...
Expanded roles of leucine-responsive regulatory protein in
... et al., 2002). A certain proportion of the regulated genes are involved, as originally proposed, in transport and metabolism of amino acids, but Lrp has also been suggested to regulate genes involved in biosynthesis and degradation of various metabolites other than amino acids (Brinkman et al., 2003 ...
... et al., 2002). A certain proportion of the regulated genes are involved, as originally proposed, in transport and metabolism of amino acids, but Lrp has also been suggested to regulate genes involved in biosynthesis and degradation of various metabolites other than amino acids (Brinkman et al., 2003 ...
Single Processing Center Models for Human Dicer and Bacterial
... tially cleaves off siRNAs from the termini of dsRNA substrates (Zhang et al., 2002). We took advantage of this observation to map cleavage sites introduced by the wt and mutant proteins in the 30 bp substrate. The 30 bp dsRNA can be cleaved by Dicer only once yielding the ⵑ21 nt siRNA-like products ...
... tially cleaves off siRNAs from the termini of dsRNA substrates (Zhang et al., 2002). We took advantage of this observation to map cleavage sites introduced by the wt and mutant proteins in the 30 bp substrate. The 30 bp dsRNA can be cleaved by Dicer only once yielding the ⵑ21 nt siRNA-like products ...
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
... et al., 1996) have shown that removal of the NH2-terminal 148 amino acids enhanced the NTPase activity of JEV NTPase/helicase. On the other hand, a comparative study with recombinant full-length NS3 and its isolated NH2- and COOH-terminal domains, performed by Gallinari et al. (1998), did not reveal ...
... et al., 1996) have shown that removal of the NH2-terminal 148 amino acids enhanced the NTPase activity of JEV NTPase/helicase. On the other hand, a comparative study with recombinant full-length NS3 and its isolated NH2- and COOH-terminal domains, performed by Gallinari et al. (1998), did not reveal ...
File
... The past 20 years have witnessed an explosion of knovvlcdge in biology, especially at the molecular level. The application of new experimental techniques for the isolation and manipulation of specific fragments of DNA has greatly increased our understanding of the processes responsible for the repl ...
... The past 20 years have witnessed an explosion of knovvlcdge in biology, especially at the molecular level. The application of new experimental techniques for the isolation and manipulation of specific fragments of DNA has greatly increased our understanding of the processes responsible for the repl ...
Stringent Response in Myxococcus xanthus
... Starve for amino acids Fruiting body formation Starve for amino acids Accumulation of (p)ppGpp Accumulate (p)ppGpp ??Starve for aa Fruiting bodies Is this a causal relationship? Is there a connection between initiation of fruiting body development and (p)ppGpp accumulation? ...
... Starve for amino acids Fruiting body formation Starve for amino acids Accumulation of (p)ppGpp Accumulate (p)ppGpp ??Starve for aa Fruiting bodies Is this a causal relationship? Is there a connection between initiation of fruiting body development and (p)ppGpp accumulation? ...
Histones - scientia.global
... DNA. They are destroyed when the S phase is complete or are rapidly degraded during the S phase if DNA replication is cut short. This type of regulation also requires specialised factors made specifically for these histone mRNAs. As mentioned above, since they have a unique 3′ end, histone mRNAs req ...
... DNA. They are destroyed when the S phase is complete or are rapidly degraded during the S phase if DNA replication is cut short. This type of regulation also requires specialised factors made specifically for these histone mRNAs. As mentioned above, since they have a unique 3′ end, histone mRNAs req ...
WS Procs 975 x 65
... dependency. Although it has a lower descriptive power than SPSP representation, experimental results on real biological data showed that it has almost the same performance as SPSP representation. Besides, since DPS representation uses fewer parameters to describe a motif, it is possible to find the ...
... dependency. Although it has a lower descriptive power than SPSP representation, experimental results on real biological data showed that it has almost the same performance as SPSP representation. Besides, since DPS representation uses fewer parameters to describe a motif, it is possible to find the ...
Nucleosides, Nucleotides,Nucleic Acids
... Watson and Crick proposed a double-stranded structure for DNA in which a purine or pyrimidine base in one chain is hydrogen bonded to its complement in the other. •Gives proper Chargaff ratios (A=T and G=C) •Because each pair contains one purine and one pyrimidine, the A---T and G---C distances betw ...
... Watson and Crick proposed a double-stranded structure for DNA in which a purine or pyrimidine base in one chain is hydrogen bonded to its complement in the other. •Gives proper Chargaff ratios (A=T and G=C) •Because each pair contains one purine and one pyrimidine, the A---T and G---C distances betw ...
Eukaryotic transcription
Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Unlike prokaryotic RNA polymerase that initiates the transcription of all different types of RNA, RNA polymerase in eukaryotes (including humans) comes in three variations, each encoding a different type of gene. A eukaryotic cell has a nucleus that separates the processes of transcription and translation. Eukaryotic transcription occurs within the nucleus where DNA is packaged into nucleosomes and higher order chromatin structures. The complexity of the eukaryotic genome necessitates a great variety and complexity of gene expression control.