
Structure and function of Escherichia coli valine transfer RNA in
... located in the anticodon loop, possesses the necessary information to recognize the codon on mRNA. There are 15 invariant positions that always contain the same base, and eight semiinvariant positions that always have either a purine or a pyrimidine (Figure 1 A). The chain length of the known tRNAs ...
... located in the anticodon loop, possesses the necessary information to recognize the codon on mRNA. There are 15 invariant positions that always contain the same base, and eight semiinvariant positions that always have either a purine or a pyrimidine (Figure 1 A). The chain length of the known tRNAs ...
Carnitine metabolism and biosynthesis in yeast Saccharomyces
... carnitine shuttle, is supported by the activities of carnitine acyltransferases and carnitine/acylcarnitine transporters, and is reasonably well studied and understood. While this function remains the only metabolically well established role of carnitine, several studies have been reporting benefici ...
... carnitine shuttle, is supported by the activities of carnitine acyltransferases and carnitine/acylcarnitine transporters, and is reasonably well studied and understood. While this function remains the only metabolically well established role of carnitine, several studies have been reporting benefici ...
Identification and Cloning of a Cryptococcal Deacetylase That
... molecular mass of 25 kDa was found to produce DTH, as evidenced by increased footpad swelling in mice inununized with culture supernatants, relative to uninununized mice. The 20-amino-acid N-tenninal sequence of thè 25-kDa protein was used to search data of thè C. neoformans Genome Project. Based on ...
... molecular mass of 25 kDa was found to produce DTH, as evidenced by increased footpad swelling in mice inununized with culture supernatants, relative to uninununized mice. The 20-amino-acid N-tenninal sequence of thè 25-kDa protein was used to search data of thè C. neoformans Genome Project. Based on ...
Structural elements defining elongation factor Tu mediated
... [3H]-labeled aa-tRNA and EF-Tu GTP in concentrations ranging from 0.02 to 2 mM in 50 mM Na-Hepes pH 7.2, 5 mM NH4Cl, 50 mM KCl, 10 mM MgCl2 and 1 mM GTP. After 5 min incubation at 48C, the mixture was filtered on a nitrocellulose disc presoaked in washing buffer. Filters were washed with 400 ml of r ...
... [3H]-labeled aa-tRNA and EF-Tu GTP in concentrations ranging from 0.02 to 2 mM in 50 mM Na-Hepes pH 7.2, 5 mM NH4Cl, 50 mM KCl, 10 mM MgCl2 and 1 mM GTP. After 5 min incubation at 48C, the mixture was filtered on a nitrocellulose disc presoaked in washing buffer. Filters were washed with 400 ml of r ...
Nucleic acid vaccines for ehrlichia chaffeensis and methods of use
... disease caused by members of the tribe Ehrlichiae have been described. Ehrlichiae infect leukocytes and endothelial cells of many different mammalian species, some of them causing serious human and veterinary diseases. Over 400 cases of ...
... disease caused by members of the tribe Ehrlichiae have been described. Ehrlichiae infect leukocytes and endothelial cells of many different mammalian species, some of them causing serious human and veterinary diseases. Over 400 cases of ...
Elucidating Flux Regulation of the Fermentation Modes of
... and IL1403 as well as the transcriptional activity of the enzymes in the mixed-acid fermentation pathways in the two strains. Chapter 3 reports the findings of the modulation of the expression of pyruvate formate-lyase and the control analysis of it in MG1363. Chapter 4 presents the characterization ...
... and IL1403 as well as the transcriptional activity of the enzymes in the mixed-acid fermentation pathways in the two strains. Chapter 3 reports the findings of the modulation of the expression of pyruvate formate-lyase and the control analysis of it in MG1363. Chapter 4 presents the characterization ...
DMD #620 1 Identification of di (2-ethylhexyl) phthalate
... 1994), were raised in male Japanese white rabbits (2.5 – 3.0 kg in body weight) according to a previously described procedure (Hosokawa et al., 1987). Anti-CES RL1 antibodies and anti-CES D1 antibodies were prepared as previously described (Hosokawa et al., 1990; Hosokawa et al., 2001). The IgG frac ...
... 1994), were raised in male Japanese white rabbits (2.5 – 3.0 kg in body weight) according to a previously described procedure (Hosokawa et al., 1987). Anti-CES RL1 antibodies and anti-CES D1 antibodies were prepared as previously described (Hosokawa et al., 1990; Hosokawa et al., 2001). The IgG frac ...
Food/feed and environmental risk assessment of insect
... Genetically modified maize 1507 x 59122 has previously been risk assessed by the VKM Panel on Genetically Modified Organisms (GMO), commissioned by the Norwegian Food Safety Authority and the Norwegian Environment Agency related to the EFSAs public hearing of the applications EFSA/GMO/NL/2005/15 and ...
... Genetically modified maize 1507 x 59122 has previously been risk assessed by the VKM Panel on Genetically Modified Organisms (GMO), commissioned by the Norwegian Food Safety Authority and the Norwegian Environment Agency related to the EFSAs public hearing of the applications EFSA/GMO/NL/2005/15 and ...
Functional and Biochemical Analysis of Glucose-6
... is genetically heterogeneous with 217 mutations reported, which have been mainly found in the coding regions and are buried in the enzyme, producing functionally-deficient G6PD variants [2]. This enzymopathy is usually diagnosed and classified through hematological studies and classified based on th ...
... is genetically heterogeneous with 217 mutations reported, which have been mainly found in the coding regions and are buried in the enzyme, producing functionally-deficient G6PD variants [2]. This enzymopathy is usually diagnosed and classified through hematological studies and classified based on th ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
... salinities above 4.5% NaCl, reaching a value of 3.34 μmol/mg protein at 6.0% NaCl. In response to salt stress, A. fulgidus VC-16S accumulated mainly diglycerol phosphate, an osmolyte thus far confined to the Archaeoglobales. Furthermore, the level of diglycerol phosphate increased approximately 28-f ...
... salinities above 4.5% NaCl, reaching a value of 3.34 μmol/mg protein at 6.0% NaCl. In response to salt stress, A. fulgidus VC-16S accumulated mainly diglycerol phosphate, an osmolyte thus far confined to the Archaeoglobales. Furthermore, the level of diglycerol phosphate increased approximately 28-f ...
Complete genome sequence of the prototype lactic acid bacterium
... of genomic islands) (69), based on the scoring of codon frequencies combined with cluster analysis. Alien genes are defined as those that are potentially acquired through horizontal gene transfer (HGT). This program has also been used to analyze the genome sequence of Bacillus licheniformis DSM13 (1 ...
... of genomic islands) (69), based on the scoring of codon frequencies combined with cluster analysis. Alien genes are defined as those that are potentially acquired through horizontal gene transfer (HGT). This program has also been used to analyze the genome sequence of Bacillus licheniformis DSM13 (1 ...
MITOCHONDRIAL DNA REPLICATION IN PRE
... 4.7.1 High oocyte mtDNA copy numbers improve fertilisation outcomes ................138 4.7.2 Delayed mtDNA replication occurs in BCB-oocytes due to delayed expression of nuclear-encoded mtDNA replication factors..........................................................139 4.7.3 Continued mtDNA repl ...
... 4.7.1 High oocyte mtDNA copy numbers improve fertilisation outcomes ................138 4.7.2 Delayed mtDNA replication occurs in BCB-oocytes due to delayed expression of nuclear-encoded mtDNA replication factors..........................................................139 4.7.3 Continued mtDNA repl ...
Fast evolutionary rates associated with functional loss in class I
... the other specific to parasitic Platyhelminthes. To study the evolutionary paths of both groups and their functional implications, we determined evolutionary rates, relative divergence times, genomic organization and performed structural analyses with the protein sequences. We finally used the model ...
... the other specific to parasitic Platyhelminthes. To study the evolutionary paths of both groups and their functional implications, we determined evolutionary rates, relative divergence times, genomic organization and performed structural analyses with the protein sequences. We finally used the model ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... approach, the mAb is first selectively captured by binding to the ELP-Protein A fusion, followed by precipitation of the complex. The precipitant is then re-solubilized in an appropriate elution buffer to dissociate the ELP-Protein AmAb complex, followed by a final precipitation of the dissociated ELP ...
... approach, the mAb is first selectively captured by binding to the ELP-Protein A fusion, followed by precipitation of the complex. The precipitant is then re-solubilized in an appropriate elution buffer to dissociate the ELP-Protein AmAb complex, followed by a final precipitation of the dissociated ELP ...
Identification and first characterization of pairing
... http://www.who.int/mediacentre/factsheets/fs115/en), while approximately 800 million are at risk (Ross et al., 2012). Besides the resulting and underestimated socio economic importance (King, 2010), schistosomes are also of basic economic relevance as they can infect animals as well (Quack et al., 2 ...
... http://www.who.int/mediacentre/factsheets/fs115/en), while approximately 800 million are at risk (Ross et al., 2012). Besides the resulting and underestimated socio economic importance (King, 2010), schistosomes are also of basic economic relevance as they can infect animals as well (Quack et al., 2 ...
Chapter 4: Cellular Metabolism
... (Outcome 4.20) 10. To complete protein synthesis, mRNA leaves_____________ and associates with _________________________________________________ . (Outcome 4.19) 11. Translation is _____________________________________ . F. Protein Synthesis (Outcome 4.20) 1. Transfer RNA functions to ______________ ...
... (Outcome 4.20) 10. To complete protein synthesis, mRNA leaves_____________ and associates with _________________________________________________ . (Outcome 4.19) 11. Translation is _____________________________________ . F. Protein Synthesis (Outcome 4.20) 1. Transfer RNA functions to ______________ ...
UNIVERSITAT ROVIRA I VIRGILI CODON USAGE ADAPTATION IN PROKARYOTIC GENOMES
... Translational selection and highly expressed genes Since the first nucleic acid sequences were sequenced and compared, several hypotheses about the evolution of genes and genomes have been proposed. Today, with the availability of a vast amount of sequences of proteins, genes and even genomes from a ...
... Translational selection and highly expressed genes Since the first nucleic acid sequences were sequenced and compared, several hypotheses about the evolution of genes and genomes have been proposed. Today, with the availability of a vast amount of sequences of proteins, genes and even genomes from a ...
ASD v2.0: updated content and novel features
... In the new version of ASD 2.0, the allosteric proteins and allosteric modulators deposited have been approximately trebled to 1286 and 22 008, the allosteric interactions and allosteric diseases increased to 23 099 and 565, respectively, and for the first time, 218 unique allosteric site structures a ...
... In the new version of ASD 2.0, the allosteric proteins and allosteric modulators deposited have been approximately trebled to 1286 and 22 008, the allosteric interactions and allosteric diseases increased to 23 099 and 565, respectively, and for the first time, 218 unique allosteric site structures a ...
Analysis of the Arabidopsis rsr4-1/pdx1
... reduced capability to oligomerize, potentially suggesting that oligomerization is important for function. In summary, our results demonstrate the critical function of the PDX1 protein family for metabolism, whole-plant development, and vitamin B6 biosynthesis in higher plants. ...
... reduced capability to oligomerize, potentially suggesting that oligomerization is important for function. In summary, our results demonstrate the critical function of the PDX1 protein family for metabolism, whole-plant development, and vitamin B6 biosynthesis in higher plants. ...
pdf
... The gammaproteobacterium Nitrosococcus oceani (ATCC 19707) is a gram-negative obligate chemolithoautotroph capable of extracting energy and reducing power from the oxidation of ammonia to nitrite. Sequencing and annotation of the genome revealed a single circular chromosome (3,481,691 bp; GⴙC conten ...
... The gammaproteobacterium Nitrosococcus oceani (ATCC 19707) is a gram-negative obligate chemolithoautotroph capable of extracting energy and reducing power from the oxidation of ammonia to nitrite. Sequencing and annotation of the genome revealed a single circular chromosome (3,481,691 bp; GⴙC conten ...
The Origin and Evolution of the Genetic Code
... There is strong support for the idea that code evolution proceeds through an ambiguous intermediate. The molecular basis for certain code changes can be elucidated: in ciliates, the mutations in the release factor eRF1 that prevent stop codon recognition can be recaptured by statistical techniques a ...
... There is strong support for the idea that code evolution proceeds through an ambiguous intermediate. The molecular basis for certain code changes can be elucidated: in ciliates, the mutations in the release factor eRF1 that prevent stop codon recognition can be recaptured by statistical techniques a ...
please refer to anzfa`s guide to applications and proposals for a
... required to be labelled as genetically modified if novel DNA and/or novel protein is present in the final food. Studies conducted by the Applicant show that novel proteins are present in the grain. Soybean DP-356043-5 has elevated levels of two minor fatty acids, heptadecanoic acid (C17:0) and hepta ...
... required to be labelled as genetically modified if novel DNA and/or novel protein is present in the final food. Studies conducted by the Applicant show that novel proteins are present in the grain. Soybean DP-356043-5 has elevated levels of two minor fatty acids, heptadecanoic acid (C17:0) and hepta ...
Mechanisms underlying inhibition of muscle disuse
... epigenetic modification. In particular, the expression levels of genes encoding cell cycle regulatory-, pro-survival and chromatin remodelling proteins, such as serine/threonine-protein kinase Chk1, cell division protein kinase 2, survivin, vesicular overexpressed in cancer prosurvival protein 1 and ...
... epigenetic modification. In particular, the expression levels of genes encoding cell cycle regulatory-, pro-survival and chromatin remodelling proteins, such as serine/threonine-protein kinase Chk1, cell division protein kinase 2, survivin, vesicular overexpressed in cancer prosurvival protein 1 and ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.