Vanillin formation from ferulic acid in Vanilla planifolia is catalysed
... involved in vanillin glucoside biosynthesis in V. planifolia, a combination of transcriptomic and proteomic approaches was undertaken with an initial focus on candidates representing the five major enzyme families suggested from the literature to play a possible role in vanillin biosynthesis, namely ...
... involved in vanillin glucoside biosynthesis in V. planifolia, a combination of transcriptomic and proteomic approaches was undertaken with an initial focus on candidates representing the five major enzyme families suggested from the literature to play a possible role in vanillin biosynthesis, namely ...
Biosynthesis of geranial, a potent aroma compound in ginger
... compounds are structurally similar, they differ in aroma properties and threshold level, indicating that their composition reflects the aroma quality of whole ginger rhizome. The contents and composition of these compounds are dependent on the ginger cultivar, and variable according to cultivation a ...
... compounds are structurally similar, they differ in aroma properties and threshold level, indicating that their composition reflects the aroma quality of whole ginger rhizome. The contents and composition of these compounds are dependent on the ginger cultivar, and variable according to cultivation a ...
NAGS - BMC Biochemistry
... upon occasional exposure to air during drought [8-11] and low tides [12,13]. Two metabolic pathways, the urea cycle and urate pathway function in the liver to efficiently convert ammonia into urea and uric acid, which are nontoxic and easily excreted [14]. Based on the type of predominant nitrogen w ...
... upon occasional exposure to air during drought [8-11] and low tides [12,13]. Two metabolic pathways, the urea cycle and urate pathway function in the liver to efficiently convert ammonia into urea and uric acid, which are nontoxic and easily excreted [14]. Based on the type of predominant nitrogen w ...
Systems Biotechnology of Pseudomonas putida for the enhanced
... Oil-based plastic production processes have given rise to several environmental problems and energy availability. Over the past 30 years, polyhydroxyalkanoates (PHA) have become one of the main sustainable alternatives to replace petroleum-base commodities. These biopolymers have superior features t ...
... Oil-based plastic production processes have given rise to several environmental problems and energy availability. Over the past 30 years, polyhydroxyalkanoates (PHA) have become one of the main sustainable alternatives to replace petroleum-base commodities. These biopolymers have superior features t ...
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
... pharmaceutical and cosmetics industries. Its demand has been growing steadily. The most remarkable use of L-threonine is as a feed additive, as recent studies have acknowledged L-threonine as the second limiting amino acid in swine feeds after L-lysine and the third limiting amino acid in poultry fe ...
... pharmaceutical and cosmetics industries. Its demand has been growing steadily. The most remarkable use of L-threonine is as a feed additive, as recent studies have acknowledged L-threonine as the second limiting amino acid in swine feeds after L-lysine and the third limiting amino acid in poultry fe ...
Calreticulin, a multi-process calcium
... The ER is the first compartment in the secretory pathway. Approx. 30 % of all cellular proteins that are synthesized into the ER, where they interact with molecular chaperones and are transported as cargo through the ER to different intracellular destinations as well as to the extracellular environm ...
... The ER is the first compartment in the secretory pathway. Approx. 30 % of all cellular proteins that are synthesized into the ER, where they interact with molecular chaperones and are transported as cargo through the ER to different intracellular destinations as well as to the extracellular environm ...
Databases - HSLS - University of Pittsburgh
... http://www.hsls.pitt.edu/molbio/videos/play?v=19 http://www.hsls.pitt.edu/guides/genetics ...
... http://www.hsls.pitt.edu/molbio/videos/play?v=19 http://www.hsls.pitt.edu/guides/genetics ...
6.
... prevalent in mammalian genomes. Using ESTs and cDNAs aligned to the genomic sequence, these studies estimate that between 35% and 59% of all human genes undergo alternative splicing [1,2]. However, it is not clear how many of the splice variants predicted from ESTs are functional and how many repres ...
... prevalent in mammalian genomes. Using ESTs and cDNAs aligned to the genomic sequence, these studies estimate that between 35% and 59% of all human genes undergo alternative splicing [1,2]. However, it is not clear how many of the splice variants predicted from ESTs are functional and how many repres ...
Hormone sensitive lipase: structure, function and
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
... acids (FFA). In addition, it has hydrolyzing activity against cholesterol esters. In this sight it is not remarkable that HSL is not only found in adipose tissue, but also in tissues that store cholesterol esters. In these tissues HSL plays a key role in the cholesterol metabolism. HSL is regulated ...
Data mining for important amino acid residues in multiple sequence
... products which induce more than one transition state in this particular reaction. As enzymes are such outstanding bio-catalysts, one of the most important goals in biochemical research is to determine the function of an enzyme (and how exactly such an enzyme catalyzes its chemical reaction). Because ...
... products which induce more than one transition state in this particular reaction. As enzymes are such outstanding bio-catalysts, one of the most important goals in biochemical research is to determine the function of an enzyme (and how exactly such an enzyme catalyzes its chemical reaction). Because ...
Raines, ChemRev 1998
... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
post- translational modifications
... We are pleased to present a selection of our building blocks for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practicall ...
... We are pleased to present a selection of our building blocks for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practicall ...
Sucrose breakdown in the potato tuber - publish.UP
... understanding of the regulation of this pathway, different strategies were followed: (I) generation and biochemical analysis of potato plants expressing a yeast invertase in their tubers (a) in the cytosol in an inducible manner and (b) in the vacuole; (II) the simulation of this pathway in silico u ...
... understanding of the regulation of this pathway, different strategies were followed: (I) generation and biochemical analysis of potato plants expressing a yeast invertase in their tubers (a) in the cytosol in an inducible manner and (b) in the vacuole; (II) the simulation of this pathway in silico u ...
Functional characterization of rare FOXP2 variants in
... the FOXP2 sequence in individuals with speech/language-related disorders have identified several rare protein-altering variants, but their phenotypic relevance is often unclear. FOXP2 encodes a transcription factor with a forkhead box DNA-binding domain, but little is known about the functions of pr ...
... the FOXP2 sequence in individuals with speech/language-related disorders have identified several rare protein-altering variants, but their phenotypic relevance is often unclear. FOXP2 encodes a transcription factor with a forkhead box DNA-binding domain, but little is known about the functions of pr ...
Solution Structure of the Tandem Acyl Carrier Protein Domains from
... ketoreductase (KR) domain; pfaB consists of a single AT domain; pfaC contains two KS homology domains and two DH domains; pfaD contains a single enoyl reductase (ER) domain and pfaE encodes for a phosphopantetheinyl transferase which is typically required for the activation of the ACP domains [3,4]. ...
... ketoreductase (KR) domain; pfaB consists of a single AT domain; pfaC contains two KS homology domains and two DH domains; pfaD contains a single enoyl reductase (ER) domain and pfaE encodes for a phosphopantetheinyl transferase which is typically required for the activation of the ACP domains [3,4]. ...
Mechanisms of animal diapause: recent
... through the dauer stage (97). Both of these dauer-suppressible phenotypes suggest that as the animal transits through dauer, the various pathways that are active during this state affect gene activity in an unprecedented manner; by altering the global genomic readout of the animal. In specific conte ...
... through the dauer stage (97). Both of these dauer-suppressible phenotypes suggest that as the animal transits through dauer, the various pathways that are active during this state affect gene activity in an unprecedented manner; by altering the global genomic readout of the animal. In specific conte ...
A Comparative Study - Carlos Ibanez Lab @ KI
... Production and quantitation of recombinant NGF Genomic fragments containing the preproNGF coding sequences from the human (see Materials and Methods), rat (Whittemore et al., 1988), and chicken (Ebendal et al., 1986) NGF genes were subcloned in the expression plasmid vector pXM (Yang et al., 1986) a ...
... Production and quantitation of recombinant NGF Genomic fragments containing the preproNGF coding sequences from the human (see Materials and Methods), rat (Whittemore et al., 1988), and chicken (Ebendal et al., 1986) NGF genes were subcloned in the expression plasmid vector pXM (Yang et al., 1986) a ...
Hydrolyzed Source Proteins as Used in Cosmetics
... The ingredients in this group are interrelated because they each are prepared from proteins by partial hydrolysis to yield cosmetically acceptable raw materials. The definitions exclude the complete hydrolysis products of proteins, the amino acids. These protein derivatives are prepared by subjectin ...
... The ingredients in this group are interrelated because they each are prepared from proteins by partial hydrolysis to yield cosmetically acceptable raw materials. The definitions exclude the complete hydrolysis products of proteins, the amino acids. These protein derivatives are prepared by subjectin ...
Purification of Arrestin from Bovine Retinas
... freshly bleached phosphorylated rhodopsin occurs rapidly (<200 msec) and quenches the activation of G protein (transducin) (1-4). We have shown that arrestin also acts in the phototransduction process by blocking rapid dephosphorylation of phosphorylated and photolyzed rhodopsin until activated rhod ...
... freshly bleached phosphorylated rhodopsin occurs rapidly (<200 msec) and quenches the activation of G protein (transducin) (1-4). We have shown that arrestin also acts in the phototransduction process by blocking rapid dephosphorylation of phosphorylated and photolyzed rhodopsin until activated rhod ...
Functional characterization of the Mycobacterium
... Eukaryotic-like Ser/Thr protein kinases (STPKs) are present in many bacterial species, where they control various physiological and virulence processes by enabling microbial adaptation to specific environmental signals. PknJ is the only member of the 11 STPKs identified in Mycobacterium tuberculosis ...
... Eukaryotic-like Ser/Thr protein kinases (STPKs) are present in many bacterial species, where they control various physiological and virulence processes by enabling microbial adaptation to specific environmental signals. PknJ is the only member of the 11 STPKs identified in Mycobacterium tuberculosis ...
Advanced Stochastic Protein Sequence Analysis
... to traditional research in molecular biology, i.e. explorations in the so-called wet labs, insilico investigations are mostly cheaper and faster by some orders of magnitude. Here, the term in-silico stands for experiments using bioinformatics methods on computers. Thus, contrary to traditional resea ...
... to traditional research in molecular biology, i.e. explorations in the so-called wet labs, insilico investigations are mostly cheaper and faster by some orders of magnitude. Here, the term in-silico stands for experiments using bioinformatics methods on computers. Thus, contrary to traditional resea ...
Analysis of the genetic potential of the spongederived fungus
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... (1,2). The L10 protein binds to ribosomal RNA (rRNA) (nucleotides 1030–1124 in Escherichia coli) next to the protein L11 at the base of the stalk (3,4). L12 is the only multicopy protein on the ribosome (5), its number varying from species to species (1). In E. coli, two L12 dimers and one L10 prote ...
... (1,2). The L10 protein binds to ribosomal RNA (rRNA) (nucleotides 1030–1124 in Escherichia coli) next to the protein L11 at the base of the stalk (3,4). L12 is the only multicopy protein on the ribosome (5), its number varying from species to species (1). In E. coli, two L12 dimers and one L10 prote ...
Lessons from the Genome Sequence of Neurospora crassa: Tracing
... Department of Plant Pathology, University of California, Riverside, California 925211; Department of Chemistry, California State Polytechnic University, Pomona, California 917682; Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The He ...
... Department of Plant Pathology, University of California, Riverside, California 925211; Department of Chemistry, California State Polytechnic University, Pomona, California 917682; Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The He ...
Developement of strategies for the isotopic labeling of methyl
... Indeed, proteins as large as 1 MDa could be investigated by NMR. Conversely, this strategy presents an important drawback consisting of the drastically reduced number of protonated probes. The project of this thesis falls within the framework of developing new methodologies to cope with this scarce ...
... Indeed, proteins as large as 1 MDa could be investigated by NMR. Conversely, this strategy presents an important drawback consisting of the drastically reduced number of protonated probes. The project of this thesis falls within the framework of developing new methodologies to cope with this scarce ...
Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA.The process of gene expression is used by all known life - eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses - to generate the macromolecular machinery for life.Several steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-translational modification of a protein. Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism.In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. The genetic code stored in DNA is ""interpreted"" by gene expression, and the properties of the expression give rise to the organism's phenotype. Such phenotypes are often expressed by the synthesis of proteins that control the organism's shape, or that act as enzymes catalysing specific metabolic pathways characterising the organism.