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Regulation of the phosphotransferase system (PTS)
Regulation of the phosphotransferase system (PTS)

... The first step of glycolysis involves activation of glucose via phosphorylation to glucose-6phosphate. This process is catalysed by a hexokinase or in the majority of bacteria this phosphorylation often occurs during the uptake of the sugar into the cell, as it will be described more explicitly belo ...
Intra- and Intergeneric Similarities of Pseudomonas and
Intra- and Intergeneric Similarities of Pseudomonas and

... temperature (Flavobacterium and Aplanobacter only). The compositions of the growth media used are listed in Table 2. In some cultures we discovered two different colony types, which we named t, and t2; when these two types displayed different soluble protein electropherograms (K. Kersters, unpublish ...
MITOCHONDRIAL DNA REPLICATION IN PRE
MITOCHONDRIAL DNA REPLICATION IN PRE

... 2.1.3 Cryopreservation of cultured cells.......................................................................51 2.1.4 Spontaneous ESC differentiation and embryoid body production ......................52 2.1.5 Directed production of embryoid bodies and ESC differentiation ......................52 ...
Analysis and engineering of acetyl
Analysis and engineering of acetyl

... tool that has recently become afiailable is the CRISPR/Cas9 system. his system consists of a Cas9 nffclease and a so-called gffideRNA (gRNA). he gRNA gffides the nffclease to a speci c, complementary, seqffence of DNA, flhere Cas9 then introdffces a doffblestrand break. he gRNA-dependence of the Cas9 nffclease ...
Saccharomyces species in the Production of Beer
Saccharomyces species in the Production of Beer

... come from the genus Saccharomyces—a minority of non-Saccharomyces yeast cultures employed in brewing will be discussed in Section 8. Yeast is cultured in an acidic aqueous sugary solution called wort prepared from barley malt and other cereals such as corn (maize), wheat, rice, sorghum, and also can ...
Molecular Mechanisms of Inhibition of Streptococcus Species by
Molecular Mechanisms of Inhibition of Streptococcus Species by

... Many of these adhesins are anchored to the cell wall peptidoglycan via their C-terminus or to the cell membrane via their N-terminal lipid (lipoproteins), and other adhesins remain surface localized through non-covalent interactions with other proteins or polysaccharides on the cell surface [6,44]. ...
Electron Transport Chains of Lactic Acid Bacteria
Electron Transport Chains of Lactic Acid Bacteria

... the fermented food products, and the positive (probiotics) or negative (pathogens) effects of their interaction with the human body. By studying bacteria derived from fermentations, a more controlled fermentation process may be achieved and a precise manipulation of nutrient and flavor production is ...
Deoxyribonucleic Acid Homology Studies of Lactobacillus casei
Deoxyribonucleic Acid Homology Studies of Lactobacillus casei

... subsp. casei. In a more comprehensive study, Dellaglio et al. (1) found that L. casei subsp. alactosus, L. casei subsp. pseudoplantarum, L. casei subsp. tolerans, and most strains designated L . casei subsp. casei formed a single homology group distinct from the type strain of L . casei subsp. casei ...
Journal of Applied Microbiology
Journal of Applied Microbiology

... strains, not isolated from potato, have been described as atypical E. c. atroseptica because, although they produce acid from a-methylglucoside and reducing substances from sucrose like E. c. atrospetica, they differ in having the ability to grow at 37C. Some of these strains, isolated from several ...
- Synthesis Bikaner
- Synthesis Bikaner

... Whichs tatement is wrong for Kreb’s cycle ? (1) The cycle starts with condensation of acetyl group (acetyl CoA) with pyruvic acid to yield citric acid (2) There are three points in the cycle where NAD+ is reducewd to NADH + H+ (3) There is one point in the cycle where FAD+ is reduced to FADH2 (4) Du ...
- UEA Digital Repository
- UEA Digital Repository

... Autotrophic microorganisms (cbbLR / cbbLG / cbbM genes) ........................ 164 ...
dhaA - Queen`s University Belfast
dhaA - Queen`s University Belfast

Characterization of the Phosphoenolpyruvate Carboxykinase Gene
Characterization of the Phosphoenolpyruvate Carboxykinase Gene

Characterization of NAD Salvage Pathways and their Role in
Characterization of NAD Salvage Pathways and their Role in

... overall virulence. Here, we seek to characterize the substrate specificity of NiaX and PnuC in ...
SPT4, a gene important for tr
SPT4, a gene important for tr

... Boston, Mass) as previously described (Swanson et al. 1991). Probes were hybridized by the dextran sulfate method as described in the GeneScreen manual. Prior to hybridization of additional probes, membranes were stripped of old probes by incubation at 80° C in 0.15 M NaC1, 0.015 M sodium citrate, 5 ...
bacterial hemoglobin proteins - ETH E-Collection
bacterial hemoglobin proteins - ETH E-Collection

... successfully expressed ...
Interactions of TCA cycle enzymes and of the CcpA
Interactions of TCA cycle enzymes and of the CcpA

... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
De novo production of resveratrol from glucose or
De novo production of resveratrol from glucose or

... et al., 2003). Deamination of tyrosine or phenylalanine, catalyzed by tyrosine/phenylalanine ammonia lyase (TAL/PAL), is the first step of resveratrol biosynthesis pathway in plants. Transcription and enzyme activity of PAL is reduced by its product, trans-cinnamic acid (Blount et al., 2000; Bolwell ...
Snorks Lab File
Snorks Lab File

... DNA TO PROTEINS Demonstrate how the genetic information in DNA molecules provides instructions for assembling protein molecules and that this is virtually the same mechanism for all life forms. ...
Cells: building blocks of living organisms
Cells: building blocks of living organisms

... Mitochondria are energy-generating organelles (cellular power plants) ...
The Utilization of Magnesium by Certain Gram
The Utilization of Magnesium by Certain Gram

... Although the magnesium requirements for maximal growth in simple chemically defined media are some ten times greater for Gram-positive bacteria than for Gramnegative bacteria (Webb, 1949a) the magnesium contents of these organisms do not differ greatly (Webb, 19498; Rouf, 1964), and it is probable t ...
Sequence variation characteristics of D
Sequence variation characteristics of D

... inner membrane. Other proteins in this membrane include enzymes involved in mitochondrial lipid synthesis and enzymes that convert lipid substrates into forms that are subsequently metabolized in the matrix. The intermembrane space contains several enzymes that use the ATP passing out of the matrix ...
Dokumentation of Lactobacillus rhamnosus 271
Dokumentation of Lactobacillus rhamnosus 271

The impact of sperm metabolism during in vitro storage: the stallion
The impact of sperm metabolism during in vitro storage: the stallion

... Adenosine triphosphate (ATP) is the energy molecule used by all living cells. It is an organic molecule containing high phosphate bonds. Energy is harvests from ATP through the breaking of these phosphate bonds by the ATPase enzyme, leaving behind adenosine diphosphate (ADP) which is rephosphorylate ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn

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Transformation (genetics)



In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".
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