Streptococcus faecium - International Journal of Systematic and
... Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus norn. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. KARL H. SCHLEIFER* A N D RENATE KILPPER-BALZ Lehrstuhl fur Mikrobiologie, Technische Universitat Miinchen, D-8000 Miinchen 2, Fede ...
... Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus norn. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. KARL H. SCHLEIFER* A N D RENATE KILPPER-BALZ Lehrstuhl fur Mikrobiologie, Technische Universitat Miinchen, D-8000 Miinchen 2, Fede ...
KingFisher Pure DNA Plant Kit
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
An Investigation of Acetobacter aceti N5
... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
A viability-linked metagenomic analysis of
... as representing an extreme environment [17], characterized by rigorous cleaning and bioburden control regimens, controlled humidity (45 ± 5 %) and temperature (25° C), and a paucity of available nutrients. As a proactive measure to monitor cleanliness and ensure mission integrity, researchers have b ...
... as representing an extreme environment [17], characterized by rigorous cleaning and bioburden control regimens, controlled humidity (45 ± 5 %) and temperature (25° C), and a paucity of available nutrients. As a proactive measure to monitor cleanliness and ensure mission integrity, researchers have b ...
... or van der Waals, plays an important role in the stability of double stranded DNA, but not protein stability. Why is this interaction more important for DNA? Electrostatic interactions (not H-bonds, these are also important in stabilizing the folded protein). The phosphate groups on DNA (and RNA) ar ...
thymine dimers - Glen Research
... outstanding. by extreme sensitivity to sunlight and high frequency It has been clear to us for some time that of skin cancer. Polymerases encountering unrepaired researchers into DNA damage and repair would value CPD lesions are quite error-prone, presumably the ability to produce oligonucleotides c ...
... outstanding. by extreme sensitivity to sunlight and high frequency It has been clear to us for some time that of skin cancer. Polymerases encountering unrepaired researchers into DNA damage and repair would value CPD lesions are quite error-prone, presumably the ability to produce oligonucleotides c ...
Mutations lowering the phosphatase activity of HPr kinase
... phosphorylation cascade leading to the phosphorylation of carbohydrates during their transport (Postma et al., 1993). In addition, P-Ser-HPr has been reported to inhibit the activity of several non-PTS permeases (Ye and Saier, 1995; Gauthier et al., 1997; Dossonnet et al., 2000; Viana et al., 2000), ...
... phosphorylation cascade leading to the phosphorylation of carbohydrates during their transport (Postma et al., 1993). In addition, P-Ser-HPr has been reported to inhibit the activity of several non-PTS permeases (Ye and Saier, 1995; Gauthier et al., 1997; Dossonnet et al., 2000; Viana et al., 2000), ...
CH Zinc Fingers As DNA Binding Domains
... Louis. *The number is f o r proteins containing two C2C2 fingers. finger proteins can be divided into four classes (Fig. 2), (A) single C2H2, (B) triple C2H2, (C) multiple-adjacent C2H2, and (D) separated-paired C2H2 zinc finger proteins. ^^ This classification is usefiil to predict how the zinc fin ...
... Louis. *The number is f o r proteins containing two C2C2 fingers. finger proteins can be divided into four classes (Fig. 2), (A) single C2H2, (B) triple C2H2, (C) multiple-adjacent C2H2, and (D) separated-paired C2H2 zinc finger proteins. ^^ This classification is usefiil to predict how the zinc fin ...
The aconitase of Escherichia cok purification of the
... Subsequent steps were performed at 4 "C and a citrate-containing buffer (TC; Tris/citrate, 2 0 m ~ ,pH 8-0) was used to protect the enzyme. The bacteria were harvested (14000 g for 15 min), washed and resuspended (1 g wet wt ml-I) in TC buffer and then disrupted by three passes through a French pres ...
... Subsequent steps were performed at 4 "C and a citrate-containing buffer (TC; Tris/citrate, 2 0 m ~ ,pH 8-0) was used to protect the enzyme. The bacteria were harvested (14000 g for 15 min), washed and resuspended (1 g wet wt ml-I) in TC buffer and then disrupted by three passes through a French pres ...
Identification of Functional Connections Between Calmodulin and the Yeast Actin Cytoskeleton.
... (Johnston et al. 1991; Brockerhoff et al. 1994) and the other is Nuf1p (Geiser et al. 1993; Kilmartin et al. 1993; Stirling et al. 1994). Myo2p is a class V myosin that is involved in polarized growth and functionally implicated in a post-Golgi stage of the secretory pathway (Johnston et al. 1991; G ...
... (Johnston et al. 1991; Brockerhoff et al. 1994) and the other is Nuf1p (Geiser et al. 1993; Kilmartin et al. 1993; Stirling et al. 1994). Myo2p is a class V myosin that is involved in polarized growth and functionally implicated in a post-Golgi stage of the secretory pathway (Johnston et al. 1991; G ...
Molecular insights into mitochondrial transcription and its
... mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochondrial RNA polymerase, POLRMT, and its two transcription factors, TFAM and ...
... mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochondrial RNA polymerase, POLRMT, and its two transcription factors, TFAM and ...
Skeletal muscle actin mRNA. Characterization of the 3
... skeletal muscle actin mRNA. Nucleotide sequence analysis indicates the f o l lowing sequence relationships: p749 specifies codons 171 to 360; pl50 specif i e s codons 357 t o 374 together with 120 nucleotides of the 3 1 -non-translated region; pi06 specifies the l a s t actin amino acid codon, the t ...
... skeletal muscle actin mRNA. Nucleotide sequence analysis indicates the f o l lowing sequence relationships: p749 specifies codons 171 to 360; pl50 specif i e s codons 357 t o 374 together with 120 nucleotides of the 3 1 -non-translated region; pi06 specifies the l a s t actin amino acid codon, the t ...
DNA Three Way Junction Core Decorated with Amino Acids
... at the core is described. One 50 -C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 50 -C(S)-functionalization were investigated to ev ...
... at the core is described. One 50 -C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 50 -C(S)-functionalization were investigated to ev ...
Paenibacillus xanthinilyticus sp. nov., isolated from agricultural soil
... of diversity of agroecosystems. A standard dilution plating technique on R2A agar (Difco) was used to isolate bacteria from an agricultural soil cultivated with Chinese cabbage (Brassica campestris L.). After plating, cultures were incubated at 28 8C for 7 days, and a white-pigmented bacterial strai ...
... of diversity of agroecosystems. A standard dilution plating technique on R2A agar (Difco) was used to isolate bacteria from an agricultural soil cultivated with Chinese cabbage (Brassica campestris L.). After plating, cultures were incubated at 28 8C for 7 days, and a white-pigmented bacterial strai ...
International Plant Protection Convention Draft annex to ISPM 27
... Xanthomonas strains that cause citrus canker symptoms. This includes Xcc, X. fuscans subsp. aurantifolii (formerly citrus canker pathotype strains B and C) and the atypical Xcc strains A* and Aw detected in Florida (Cubero & Graham, 2002). They are universal, but they have lower sensitivity (104 cfu ...
... Xanthomonas strains that cause citrus canker symptoms. This includes Xcc, X. fuscans subsp. aurantifolii (formerly citrus canker pathotype strains B and C) and the atypical Xcc strains A* and Aw detected in Florida (Cubero & Graham, 2002). They are universal, but they have lower sensitivity (104 cfu ...
File
... I did not write Introduction to A10lecular Biology to serve as a stand-alone text. Rather, the student should use it as a supplement to class lecture notes and the more comprehensive textbooks required in introductory biology courses. Students enrolled in majors' or non majors' introductory biology ...
... I did not write Introduction to A10lecular Biology to serve as a stand-alone text. Rather, the student should use it as a supplement to class lecture notes and the more comprehensive textbooks required in introductory biology courses. Students enrolled in majors' or non majors' introductory biology ...
Identification of the Gene Encoding the Tryptophan Synthase ß
... BLASTP search (Altschul et al., 1990) were to TSB sequences from other photosynthetic organisms. For example, the C. reinhardtii predicted translation product was 75% identical and 86% similar to the TRP2 precursor protein from maize (Zea mays) (P 5 2.02216). Identity and similarity to TSB from the ...
... BLASTP search (Altschul et al., 1990) were to TSB sequences from other photosynthetic organisms. For example, the C. reinhardtii predicted translation product was 75% identical and 86% similar to the TRP2 precursor protein from maize (Zea mays) (P 5 2.02216). Identity and similarity to TSB from the ...
The sequence of human serum albumin cDNA and its expression in
... recombinant plasmids contained s u f f i c i e n t l y large inserts to code for the remaining 5' portion of the HSA message. Two of these (F-15 and F-47) contained the extreme 51 coding portion of the mature protein message but f a i l e d to extend back to a Pstl s i t e necessary for joining with ...
... recombinant plasmids contained s u f f i c i e n t l y large inserts to code for the remaining 5' portion of the HSA message. Two of these (F-15 and F-47) contained the extreme 51 coding portion of the mature protein message but f a i l e d to extend back to a Pstl s i t e necessary for joining with ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Expression and purification of OE1472F, OE2019F & OE1477R ............ 100 Expression of OE1472F in H. salinarum......................................................... 100 ...
... Expression and purification of OE1472F, OE2019F & OE1477R ............ 100 Expression of OE1472F in H. salinarum......................................................... 100 ...
Internal expression of Yarrowia NDH2
... vector pINA443 (Barth and Gaillardin, 1996) by removing the URA3 marker by digestion with BamHI and SalI and replacing it with the Klebsiella pneumoniae hygromycin B phosphotransferase (HygBR) gene (Gritz and Davies, 1983) under the control of a strong hybrid promoter, consisting of four direct repe ...
... vector pINA443 (Barth and Gaillardin, 1996) by removing the URA3 marker by digestion with BamHI and SalI and replacing it with the Klebsiella pneumoniae hygromycin B phosphotransferase (HygBR) gene (Gritz and Davies, 1983) under the control of a strong hybrid promoter, consisting of four direct repe ...
As a PDF file
... connected to their biosynthetic activity. Therefore, their sugar metabolism can be engineered without substantial interference to the biosynthesis pathways. This engineering ability has increased the interest in and significance of LAB as novel hosts for the production of valuable metabolites. Durin ...
... connected to their biosynthetic activity. Therefore, their sugar metabolism can be engineered without substantial interference to the biosynthesis pathways. This engineering ability has increased the interest in and significance of LAB as novel hosts for the production of valuable metabolites. Durin ...
(lip) that - Repositories
... monoxygenases, but using sulfur rather than oxygen, would also achieve the desired result. Knight et al. (1963) showed that sulfur could be directly inserted into C-H bonds of hydrocarbons (White, 1980b). However, most monoxygenase-catalyzed hydroxylations proceed with retention of configuration, bu ...
... monoxygenases, but using sulfur rather than oxygen, would also achieve the desired result. Knight et al. (1963) showed that sulfur could be directly inserted into C-H bonds of hydrocarbons (White, 1980b). However, most monoxygenase-catalyzed hydroxylations proceed with retention of configuration, bu ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".