Promoter Analysis of the Mouse Sterol Regulatory Element
... family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concentrations. Upon sterol deprivation, the precursor protein undergoes a sequential two-step cleavage process to release the NH2-termina ...
... family, SREBPs are synthesized as precursor proteins that remain bound to the endoplasmic reticulum and the nuclear envelope in the presence of sufficient sterol concentrations. Upon sterol deprivation, the precursor protein undergoes a sequential two-step cleavage process to release the NH2-termina ...
structure and mechanism of dna polymerases
... members of this family contain additional structural elements, which may be important for function. For example, polymerase l contains a nuclear localization signal and a breast cancer susceptibility gene (BRCT) domain. The BRCT domain is thought to mediate protein–protein and protein–DNA interactio ...
... members of this family contain additional structural elements, which may be important for function. For example, polymerase l contains a nuclear localization signal and a breast cancer susceptibility gene (BRCT) domain. The BRCT domain is thought to mediate protein–protein and protein–DNA interactio ...
Prokaryotes - Hardin County Schools
... in whole or in sections must include the referral attribution link http://www.ck12.org/saythanks (placed in a visible location) in addition to the following terms. Except as otherwise noted, all CK-12 Content (including CK-12 Curriculum Material) is made available to Users in accordance with the Cre ...
... in whole or in sections must include the referral attribution link http://www.ck12.org/saythanks (placed in a visible location) in addition to the following terms. Except as otherwise noted, all CK-12 Content (including CK-12 Curriculum Material) is made available to Users in accordance with the Cre ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... sugar-phosphate backbone, including one made by the amino acid at recognition helix position 9, may contribute to the stability of the repressor-operator complex. The amino acid sequence of the Bicoid homeodomain differs from that in proteins of the Anfennapedia (An@) sequence class (Scott et al., 1 ...
... sugar-phosphate backbone, including one made by the amino acid at recognition helix position 9, may contribute to the stability of the repressor-operator complex. The amino acid sequence of the Bicoid homeodomain differs from that in proteins of the Anfennapedia (An@) sequence class (Scott et al., 1 ...
Vitis 37 (3), 119
... genetic similarity was calculated and the dendrogram of the 14 cultivars was established. The results indicate that there is genetic variation among the cultivars of the muscat family with values of the genetic similarity ranging from 0.666 to 1.00. On the basis of the observed bands it was possible ...
... genetic similarity was calculated and the dendrogram of the 14 cultivars was established. The results indicate that there is genetic variation among the cultivars of the muscat family with values of the genetic similarity ranging from 0.666 to 1.00. On the basis of the observed bands it was possible ...
The relative rates of synthesis of DNA, sRNA and rRNA in the
... The onset and rates of synthesis of the major classes of nucleic acids have been extensively studied during the development of whole frog embryos (reviews by Brown, 1965; Gurdon, 1967a). Such information is of interest because nucleic acids are the immediate products of genes, and their rates of syn ...
... The onset and rates of synthesis of the major classes of nucleic acids have been extensively studied during the development of whole frog embryos (reviews by Brown, 1965; Gurdon, 1967a). Such information is of interest because nucleic acids are the immediate products of genes, and their rates of syn ...
J Urol - prostata
... Policlinico A. Gemelli, Largo A. Gemelli, 8, 00168 Roma, Italy. [email protected] Biologic factors affect the ability of radiation to effectively treat all patients with prostate cancer. The use of prognostic and genetic markers (12 lipoxygenase, p53, bc1-2 genes, ploidy) may aid in the develop ...
... Policlinico A. Gemelli, Largo A. Gemelli, 8, 00168 Roma, Italy. [email protected] Biologic factors affect the ability of radiation to effectively treat all patients with prostate cancer. The use of prognostic and genetic markers (12 lipoxygenase, p53, bc1-2 genes, ploidy) may aid in the develop ...
CHAPTER 4 ISOLATION, CHARACTERIZATION AND EXPRESSION OF GA20ox
... The activity of EtGA20 ox1 was monitored using a gel-purified PCR product (1.2 kb) that was amplified using primers sense: 5’-AGG GAT CCA GCC AGC TGC CCG TGA TG-3’ and antisense: 5’-TGA AGC TTA ACA GAA CAG GCG GTC ATG GAT GAC-3’ from a cDNA template. The PCR amplification was done using “Phusion” po ...
... The activity of EtGA20 ox1 was monitored using a gel-purified PCR product (1.2 kb) that was amplified using primers sense: 5’-AGG GAT CCA GCC AGC TGC CCG TGA TG-3’ and antisense: 5’-TGA AGC TTA ACA GAA CAG GCG GTC ATG GAT GAC-3’ from a cDNA template. The PCR amplification was done using “Phusion” po ...
MD Simulations of the P53 oncoprotein structure
... The p53 tumor suppressor protein is involved in preventing cancer and plays a central role in conserving genomic stability by preventing a genome mutation [1-5]. p53 (the 53 kilodalton (kDa) protein) is activated either to induce a cell cycle arrest allowing the repair and survival of the cell, or a ...
... The p53 tumor suppressor protein is involved in preventing cancer and plays a central role in conserving genomic stability by preventing a genome mutation [1-5]. p53 (the 53 kilodalton (kDa) protein) is activated either to induce a cell cycle arrest allowing the repair and survival of the cell, or a ...
Colicins produced by the Escherichia fergusonii strains closely
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
PPT - Altogen Biosystems
... 3. Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
... 3. Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
Bacillus clausii Probiotic Strains
... four B. clausii strains, which are included in the probiotic Enterogermina, exhibited an antimicrobial activity in vitro. Moreover, we determined that B. clausii O/C strain produced at least one antimicrobial substance secreted in the media, which displayed an activity against the Gram-positive bact ...
... four B. clausii strains, which are included in the probiotic Enterogermina, exhibited an antimicrobial activity in vitro. Moreover, we determined that B. clausii O/C strain produced at least one antimicrobial substance secreted in the media, which displayed an activity against the Gram-positive bact ...
The KIebsieIIa pneumoniae cytochrome bd
... severely impaired in the presence of low concentrations of oxygen compared with the wild-type bacterium. Only the wild-type organism was capable of microaerobic nitrogenase activity supported by fermentation products. It is proposed that formate dehydrogenase-0may be involved in supplying electrons ...
... severely impaired in the presence of low concentrations of oxygen compared with the wild-type bacterium. Only the wild-type organism was capable of microaerobic nitrogenase activity supported by fermentation products. It is proposed that formate dehydrogenase-0may be involved in supplying electrons ...
Final Sabatini Project 2009
... may not have been allowed to sit long enough to completely absorb it. •The lag time between making each tube may have caused slightly different times for plating and absorption ...
... may not have been allowed to sit long enough to completely absorb it. •The lag time between making each tube may have caused slightly different times for plating and absorption ...
Two novel species of marine phototrophic Gammaproteobacteria
... The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of ...
... The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of ...
Nested PCR Assays for Detection of Monilinia fructicola in Stone
... the anamorphs are also similar among some Botryosphaeria species and can be strongly influenced by the ...
... the anamorphs are also similar among some Botryosphaeria species and can be strongly influenced by the ...
Isolation, cloning and sequence analysis of the lactate
... tropical theileriosis and genomic DNA was extracted following the confirmation of the clinical diagnosis. For the first time, in this study, the lactate dehydrogenase sequence was isolated from from a Theileria species. Following extraction from genomic DNA by PCR the sequence was cloned into the ve ...
... tropical theileriosis and genomic DNA was extracted following the confirmation of the clinical diagnosis. For the first time, in this study, the lactate dehydrogenase sequence was isolated from from a Theileria species. Following extraction from genomic DNA by PCR the sequence was cloned into the ve ...
Unit 10 Simple Life Forms Chp 27 Prokaryotic
... peptidoglycans, preventing the formation of a functional wall, particularly in grampositive species. ...
... peptidoglycans, preventing the formation of a functional wall, particularly in grampositive species. ...
DNAse I Qualification and Sample Treatment | Molecular Devices
... Total DNA Assay specifically measures DNA and not a non-DNA component of the sample, or To confirm that observed inhibition of the Total DNA Assay is caused by the presence of small fragment DNA, rather than protein or buffer interference. This application note describes procedures to address both of ...
... Total DNA Assay specifically measures DNA and not a non-DNA component of the sample, or To confirm that observed inhibition of the Total DNA Assay is caused by the presence of small fragment DNA, rather than protein or buffer interference. This application note describes procedures to address both of ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".