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... An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low levels of mutagenicity and toxicity compared ...
... An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low levels of mutagenicity and toxicity compared ...
Mutagenesis identifies the critical amino acid residues of human
... colicin E9, His-98 in I-PpoI, and His-80 in Vvn) act as general bases to active water molecules for the nucleophilic attacks on the phosphorus atoms [13-17]. Arginine residues (Arg-93 in NucA, Arg-57 in Serratia nuclease, Arg-5 in colicin E9, Arg-61 in I-PpoI, and Arg-99 in Vvn) donate hydrogen bond ...
... colicin E9, His-98 in I-PpoI, and His-80 in Vvn) act as general bases to active water molecules for the nucleophilic attacks on the phosphorus atoms [13-17]. Arginine residues (Arg-93 in NucA, Arg-57 in Serratia nuclease, Arg-5 in colicin E9, Arg-61 in I-PpoI, and Arg-99 in Vvn) donate hydrogen bond ...
Molecule of the Month: AgrA DNA Binding Domain AgrA is the
... seen in hospitals (where they often infected wounds and sites of surgical repair), it has recently become a growing (and concerning) problem outside of clinical situations: as many as 19,000 people die every year in the United States from MRSA infections, more than from HIV2. The Agr pathway is nece ...
... seen in hospitals (where they often infected wounds and sites of surgical repair), it has recently become a growing (and concerning) problem outside of clinical situations: as many as 19,000 people die every year in the United States from MRSA infections, more than from HIV2. The Agr pathway is nece ...
Nucleosomal structure of sea urchin and starfish sperm chromatin
... sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, seemed to be very similar as judged from their amino acid compositions and electrophoretic behavior in two systems. To verify the above we considered also the data available on the primar ...
... sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, seemed to be very similar as judged from their amino acid compositions and electrophoretic behavior in two systems. To verify the above we considered also the data available on the primar ...
p53 regulation and function in normal cells and tumors
... and chemical carcinogens. Mechanisms to protect the integrity of inherited genetic information from the consequences of such exposure include the pathway of the tumor suppressor p53 protein, a guardian of the genome. p53 is a DNA binding protein; attention has focused on its specific binding to a co ...
... and chemical carcinogens. Mechanisms to protect the integrity of inherited genetic information from the consequences of such exposure include the pathway of the tumor suppressor p53 protein, a guardian of the genome. p53 is a DNA binding protein; attention has focused on its specific binding to a co ...
Gluconacetobacter entanii sp. nov., isolated from submerged high
... 4560T, LTH 4341, LTH 4551 and LTH 4637) were characterized in more detail and it was revealed that they have in common certain properties such as requirement of acetic acid, ethanol and glucose for growth, and no overoxidation of acetate. Growth occurs only at total concentrations (sum of acetic aci ...
... 4560T, LTH 4341, LTH 4551 and LTH 4637) were characterized in more detail and it was revealed that they have in common certain properties such as requirement of acetic acid, ethanol and glucose for growth, and no overoxidation of acetate. Growth occurs only at total concentrations (sum of acetic aci ...
Full-Text PDF
... impedance for rapid detection of foodborne pathogenic bacteria [7]. An analysis of foodborne outbreak data (events in which two or more people became ill after consuming a common food or meal) reported internationally has recently been described by Greig and Ravel [8]. Using outbreak data for food a ...
... impedance for rapid detection of foodborne pathogenic bacteria [7]. An analysis of foodborne outbreak data (events in which two or more people became ill after consuming a common food or meal) reported internationally has recently been described by Greig and Ravel [8]. Using outbreak data for food a ...
Bioinformatics Molecular Genetics
... determine one's genetic predisposition for obesity. Interplay between genes and environment, the influence of many genes—appear to be the norm with regard to many and perhaps most ("complex" or "multi-factoral") traits. Phenotype: the characteristics that result from this interplay. ...
... determine one's genetic predisposition for obesity. Interplay between genes and environment, the influence of many genes—appear to be the norm with regard to many and perhaps most ("complex" or "multi-factoral") traits. Phenotype: the characteristics that result from this interplay. ...
Chapter 20 Prokaryotes
... Prokaryotes reproduce asexually by means of binary fission. – Conjugation occurs between bacteria when the donor cell passes DNA to recipient cell by way of sex pilus. ...
... Prokaryotes reproduce asexually by means of binary fission. – Conjugation occurs between bacteria when the donor cell passes DNA to recipient cell by way of sex pilus. ...
A structural determinant in the uracil DNA glycosylase superfamily
... was cloned into pET21a as described above. The recombinant plasmid (pET21a-UNG-N123A) containing the desired mutated gene was confirmed by DNA sequencing and electroporated into E. coli strain BH214 (mug− , ung− ). The E. coli MUG and UNG mutant proteins were expressed and purified as described abov ...
... was cloned into pET21a as described above. The recombinant plasmid (pET21a-UNG-N123A) containing the desired mutated gene was confirmed by DNA sequencing and electroporated into E. coli strain BH214 (mug− , ung− ). The E. coli MUG and UNG mutant proteins were expressed and purified as described abov ...
Mitochondrial DNA and aging
... III) [4]. The inheritance of mtDNA is almost exclusively maternal, although some important exceptions have been reported [5–7]. mtDNA is present in one to several thousand copies per cell [8] and is ‘encapsulated’ into mitochondria at 1–11 copies per mitochondrion with the mean being two genomes per ...
... III) [4]. The inheritance of mtDNA is almost exclusively maternal, although some important exceptions have been reported [5–7]. mtDNA is present in one to several thousand copies per cell [8] and is ‘encapsulated’ into mitochondria at 1–11 copies per mitochondrion with the mean being two genomes per ...
NSPCD LABORATORIES - World Health Organization
... Distinguish relapse from re-infection Identify types associated with increased transmission & virulence Emergence of new types ; implications on control measures Clonality of acute infection: ...
... Distinguish relapse from re-infection Identify types associated with increased transmission & virulence Emergence of new types ; implications on control measures Clonality of acute infection: ...
The pyruvate dehydrogenase complex of the chemol
... and (ii) to generate metabolites required for biosynthesis, such as the amino acids of the glutamate family. T . ferrooxidans is an obligately chemolithoautotrophic bacterium that obtains its energy by oxidizing Fe2+ to Fe3+ or reduced sulfur compounds to sulfuric acid (Kelly & Harrison, 1989). It w ...
... and (ii) to generate metabolites required for biosynthesis, such as the amino acids of the glutamate family. T . ferrooxidans is an obligately chemolithoautotrophic bacterium that obtains its energy by oxidizing Fe2+ to Fe3+ or reduced sulfur compounds to sulfuric acid (Kelly & Harrison, 1989). It w ...
5 DNA Replication
... playing the game, the more garbled the message becomes. This game illustrates an important principle: errors arise whenever information is copied; the more times it is copied, the greater the number of errors. A complex, multicellular organism faces a problem similar to that of the children in the s ...
... playing the game, the more garbled the message becomes. This game illustrates an important principle: errors arise whenever information is copied; the more times it is copied, the greater the number of errors. A complex, multicellular organism faces a problem similar to that of the children in the s ...
FEMS Microbiology Letters
... of growth. The cells were lysed with 500 mg mL1 of lysozyme in 100 mL of buffer TES [50 mM Tris-HCl, pH 8.5, 50 mM EDTA, 50 mM NaCl]. The mixture was stirred for 18 h, at 4 1C and centrifuged at 10 000 g for 20 min. The supernatant (crude extract) was brought to 30% (w/v) by adding ammonium sulfate ...
... of growth. The cells were lysed with 500 mg mL1 of lysozyme in 100 mL of buffer TES [50 mM Tris-HCl, pH 8.5, 50 mM EDTA, 50 mM NaCl]. The mixture was stirred for 18 h, at 4 1C and centrifuged at 10 000 g for 20 min. The supernatant (crude extract) was brought to 30% (w/v) by adding ammonium sulfate ...
DNA and Protein Production
... strand comes from taking the other two phosphates off. The energy gained from breaking the bonds is used to build the new bond ...
... strand comes from taking the other two phosphates off. The energy gained from breaking the bonds is used to build the new bond ...
Document
... by E.M. Southern (1975). Southern was the first to show that immobilization of sizefractionated DNA fragments could be carried out in a reliable and efficient manner. The advent of Southern transfer and the associated hybridization techniques made it possible for the first time to obtain information ...
... by E.M. Southern (1975). Southern was the first to show that immobilization of sizefractionated DNA fragments could be carried out in a reliable and efficient manner. The advent of Southern transfer and the associated hybridization techniques made it possible for the first time to obtain information ...
Altering substrate specificity of catechol 2,3
... Planococcus sp. strain S5 was randomly mutagenized to generate variant forms of the enzyme with higher degradation activity. Additionally, the effect of introduced mutations on the enzyme structure was analyzed based on the putative 3D models the wild-type and mutant enzymes. C23OB58 and C23OB81 mut ...
... Planococcus sp. strain S5 was randomly mutagenized to generate variant forms of the enzyme with higher degradation activity. Additionally, the effect of introduced mutations on the enzyme structure was analyzed based on the putative 3D models the wild-type and mutant enzymes. C23OB58 and C23OB81 mut ...
PDF - FEMS Microbiology Letters
... site was only present in the Ent. faecium RM58 decarboxylase gene sequence (accession AJ783966). This unwanted cloning event produces a truncated protein of 541 aminoacid residues, lacking 84 amino-acid residues of the Cterminus. In order to solve this cloning problem, primer 98 was designed. Follow ...
... site was only present in the Ent. faecium RM58 decarboxylase gene sequence (accession AJ783966). This unwanted cloning event produces a truncated protein of 541 aminoacid residues, lacking 84 amino-acid residues of the Cterminus. In order to solve this cloning problem, primer 98 was designed. Follow ...
Bacterial genome replication at subzero temperatures in permafrost
... CH4, but the studies were performed at higher incubation temperatures ranging from 4 to 25 1C (Martineau et al., 2010; Liebner et al., 2011; He et al., 2012). Here, we used the SIP method to elucidate how specific members of the bacterial community responded to various subzero incubation temperature ...
... CH4, but the studies were performed at higher incubation temperatures ranging from 4 to 25 1C (Martineau et al., 2010; Liebner et al., 2011; He et al., 2012). Here, we used the SIP method to elucidate how specific members of the bacterial community responded to various subzero incubation temperature ...
Diagnostic protocol for
... reach full fruiting development because when the leaves are not young and the fruits reach their final size, they are not susceptible under natural conditions and fewer angular shoots are produced. Disease severity also depends on the susceptibility of the host plant species and cultivars (Goto, 199 ...
... reach full fruiting development because when the leaves are not young and the fruits reach their final size, they are not susceptible under natural conditions and fewer angular shoots are produced. Disease severity also depends on the susceptibility of the host plant species and cultivars (Goto, 199 ...
Directions for Use Uracil-DNA Glycosylase (UNG), Cod
... UNG does not act on uracil on the ribose sugar backbone. It will work on uracilcontaining oligos as small as 5-mers. What is the difference between UNG and UDG? The E. coli enzyme Uracil DNA Glycosylase, which may also be called Uracil-NGlycosylase, is abbreviated frequently as UDG or UNG. Both ...
... UNG does not act on uracil on the ribose sugar backbone. It will work on uracilcontaining oligos as small as 5-mers. What is the difference between UNG and UDG? The E. coli enzyme Uracil DNA Glycosylase, which may also be called Uracil-NGlycosylase, is abbreviated frequently as UDG or UNG. Both ...
PPT - Altogen Biosystems
... Products > HCAEC Transfection Reagent (Coronary Artery Endothelial) Altogen Biosystems offers the HCAEC Cell Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HCAEC Cell Transfection Reagent is a nanoparticle-based liposome mediated formulation, and it has ...
... Products > HCAEC Transfection Reagent (Coronary Artery Endothelial) Altogen Biosystems offers the HCAEC Cell Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The HCAEC Cell Transfection Reagent is a nanoparticle-based liposome mediated formulation, and it has ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".