2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... order of 6 nm. In a stiffer trap these fluctuations are reduced. However, this will not necessarily be of benefit, because trap stiffness often reduces the signal measured from a DNA-protein complex with an equal factor, resulting in no net gain in signal-to-noise ratio [132]. Trap stiffness can be ...
... order of 6 nm. In a stiffer trap these fluctuations are reduced. However, this will not necessarily be of benefit, because trap stiffness often reduces the signal measured from a DNA-protein complex with an equal factor, resulting in no net gain in signal-to-noise ratio [132]. Trap stiffness can be ...
... iii) (4 pts) After constructing the expression vector, you transform the DNA into bacteria such that each bacteria receives one DNA molecule. To your surprise, you find that none of the bacterial cells produce peptide from the expression vector. Plasmid DNA is isolated from a number of different bac ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... Fig. 1. Analysis of the Protein and Nucleotide Sequences Used in this Study A, Amino acid sequence corresponding to the Cys4-Cys4 zinc finger DBD of mouse SF1. The locations of the highly conserved core DBD shared by NRs as well as the FTZ-F1 box unique to the NR5A subfamily are indicated. SF1 resid ...
... Fig. 1. Analysis of the Protein and Nucleotide Sequences Used in this Study A, Amino acid sequence corresponding to the Cys4-Cys4 zinc finger DBD of mouse SF1. The locations of the highly conserved core DBD shared by NRs as well as the FTZ-F1 box unique to the NR5A subfamily are indicated. SF1 resid ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
Assessment of the mosaic structure in the
... amplicons are sequenced using a battery of gene specific primers (often the PCR primers). In a recent study, we described an improved strategy using M13 and T7 sequence tagged primers in PCR amplification covering the cagA EPIYA motifs. Tagging of the PCR primers enables rapid sequencing using unive ...
... amplicons are sequenced using a battery of gene specific primers (often the PCR primers). In a recent study, we described an improved strategy using M13 and T7 sequence tagged primers in PCR amplification covering the cagA EPIYA motifs. Tagging of the PCR primers enables rapid sequencing using unive ...
Plant and Soil.
... nod- and nif (nitrogen-fixing) genes linked to host-plant specificity. A different grouping was obtained when chromosomal probes were analyzed, some strains of R. galegae from G. officinalis being more closely related to strains isolated from G. orientalis (Kaijalainen and Lindstr6m, 1989). Interstr ...
... nod- and nif (nitrogen-fixing) genes linked to host-plant specificity. A different grouping was obtained when chromosomal probes were analyzed, some strains of R. galegae from G. officinalis being more closely related to strains isolated from G. orientalis (Kaijalainen and Lindstr6m, 1989). Interstr ...
reactive_oxygen_species
... The loss of Nrf2 causes a decrease in GST expression and increased levels of ROS. This ultimately leads to DNA damage associated with tumorigenesis. ...
... The loss of Nrf2 causes a decrease in GST expression and increased levels of ROS. This ultimately leads to DNA damage associated with tumorigenesis. ...
Sequence Analysis of the DNA Encoding the Eco RI Endonuclease
... model system for probing the molecular mechanisms of se- methods of DNA sequencing, are identical in both thecoding and noncoding regions. We are, therefore, confident that the quence-specific DNA-protein interactions. The two enzymes (1, nucleotide sequence and the predicted amino acid sequences re ...
... model system for probing the molecular mechanisms of se- methods of DNA sequencing, are identical in both thecoding and noncoding regions. We are, therefore, confident that the quence-specific DNA-protein interactions. The two enzymes (1, nucleotide sequence and the predicted amino acid sequences re ...
Effect of RNAi down-regulation of three lysine-deficient
... content of the essential amino acids lysine and methionine. The chapter concludes by formulating a genetic engineering strategy that aims at improving the seed lysine content. The first technical effort towards achieving the main aims is covered in Chapter 2. This involves in vitro screening of five ...
... content of the essential amino acids lysine and methionine. The chapter concludes by formulating a genetic engineering strategy that aims at improving the seed lysine content. The first technical effort towards achieving the main aims is covered in Chapter 2. This involves in vitro screening of five ...
Chapter C4
... • Proteins and Traits Proteins act as chemical triggers for many of the processes within cells. Proteins help determine traits. • Help from RNA Another type of molecule that helps make proteins is called RNA, or ribonucleic acid. RNA is so similar to DNA that RNA can serve as a temporary copy of a D ...
... • Proteins and Traits Proteins act as chemical triggers for many of the processes within cells. Proteins help determine traits. • Help from RNA Another type of molecule that helps make proteins is called RNA, or ribonucleic acid. RNA is so similar to DNA that RNA can serve as a temporary copy of a D ...
Chapter 6
... form proteins of many shapes and sizes. The function of a protein depends on the shape of the bonded amino acids. If even a single amino acid is missing or out of place, the protein may not function correctly or may not function. Foods such as meat, fish, cheese, and beans contain proteins, which ar ...
... form proteins of many shapes and sizes. The function of a protein depends on the shape of the bonded amino acids. If even a single amino acid is missing or out of place, the protein may not function correctly or may not function. Foods such as meat, fish, cheese, and beans contain proteins, which ar ...
Characterization of Phosphorus Forms in Soil Microorganisms
... the growth-limiting nutrient, although the number of cells can still increase if initiated cell division is completed. Unlike for carbon, biomass production can continue due to relocation of the nutrient. For example, teichoic acids can be replaced by P-free teichuronic acids in Gram-positive bacter ...
... the growth-limiting nutrient, although the number of cells can still increase if initiated cell division is completed. Unlike for carbon, biomass production can continue due to relocation of the nutrient. For example, teichoic acids can be replaced by P-free teichuronic acids in Gram-positive bacter ...
Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... cerevisiae have been cloned from a library of yeast genomic DNA. Using an l yjtro glucose phosphorylation assay, the NKB gene was located on a plasmid carrying a 13.6 kb fragment of yeast DNA. After subeloning the relevant restriction fragments, the nucleotide sequence of the BIB gene was determined ...
... cerevisiae have been cloned from a library of yeast genomic DNA. Using an l yjtro glucose phosphorylation assay, the NKB gene was located on a plasmid carrying a 13.6 kb fragment of yeast DNA. After subeloning the relevant restriction fragments, the nucleotide sequence of the BIB gene was determined ...
mitochondrial dysfunction and treatment strategies
... cells. The daughter cells enter the G1 phase again and the process repeats itself. Cells can also enter a non-dividing state, which is either not reversible (senescence or apoptosis), or a non-proliferative phase (G0 phase, resting phase or quiescence). There are several check points in the cell cy ...
... cells. The daughter cells enter the G1 phase again and the process repeats itself. Cells can also enter a non-dividing state, which is either not reversible (senescence or apoptosis), or a non-proliferative phase (G0 phase, resting phase or quiescence). There are several check points in the cell cy ...
1. Which of the following are connected by hydrogen bonds? A
... Escherichia coli is a unicellular organism, so each cell must carry out all of the processes required for life. Outline the functions of each of the structures in the cells of Escherichia coli. ...
... Escherichia coli is a unicellular organism, so each cell must carry out all of the processes required for life. Outline the functions of each of the structures in the cells of Escherichia coli. ...
Metalloenzyme Functions
... structural and functional details on each COG and literature references, improvements of the COGNITOR program that is used to fit new proteins into the COGs, and classification of genomes and COGs constructed by ...
... structural and functional details on each COG and literature references, improvements of the COGNITOR program that is used to fit new proteins into the COGs, and classification of genomes and COGs constructed by ...
III BSC BT - 609 A -core- Animal Biotechnology
... 16. In gene therapy, the abnormal gene could be repaired through selective ___________, that returns the gene to its normal function. A. alteration. B. reverse mutation. C. removal. D. retrival. ANSWER: B 17. Restriction enzymes can be used to _____________ . A. join up bits of dna. B. inhibit the ...
... 16. In gene therapy, the abnormal gene could be repaired through selective ___________, that returns the gene to its normal function. A. alteration. B. reverse mutation. C. removal. D. retrival. ANSWER: B 17. Restriction enzymes can be used to _____________ . A. join up bits of dna. B. inhibit the ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".