PPT - Altogen Biosystems
... 3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
... 3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium 4. Incubate transfection complexes at RT for 15 - 30 minutes 5. Optional: ...
Eurofins Legionella PCR (Polymerase Chain Reaction) Technical
... Advantages of Using the Legionella PCR Method Rather than the Culture Technique The traditional Legionella culture technique takes 10 days to arrive at a confirmed negative result as Legionella bacteria are slow growing in the laboratory. A further 3 days are required for confirmation in the case of ...
... Advantages of Using the Legionella PCR Method Rather than the Culture Technique The traditional Legionella culture technique takes 10 days to arrive at a confirmed negative result as Legionella bacteria are slow growing in the laboratory. A further 3 days are required for confirmation in the case of ...
PPT - Altogen Biosystems
... time of 52 hours and a chromosomal modal number of 63. The cell line can be used as an in vitro model in tumorigenicity studies related to non-endocrine pancreatic cancer. L-asparagine inhibits growth of PANC-1 cells. FGF2 stimulates the cell line to differentiate into islet like clusters that produ ...
... time of 52 hours and a chromosomal modal number of 63. The cell line can be used as an in vitro model in tumorigenicity studies related to non-endocrine pancreatic cancer. L-asparagine inhibits growth of PANC-1 cells. FGF2 stimulates the cell line to differentiate into islet like clusters that produ ...
Lab IV: Recombinant DNA Analysis
... prevents DNA recombination from occurring, so a transformed plasmid will remain extrachromosomal and stable. Second, the lacZ mutation ( = a deletion in the listed gene) means that DH5 does not have a functional lacZ gene, one of the three genes encoded by the lac operon. The LacZ enzyme is norma ...
... prevents DNA recombination from occurring, so a transformed plasmid will remain extrachromosomal and stable. Second, the lacZ mutation ( = a deletion in the listed gene) means that DH5 does not have a functional lacZ gene, one of the three genes encoded by the lac operon. The LacZ enzyme is norma ...
The first true obligately syntrophic propionate
... resulted in a product of the expected size of 1?4 kb. This fragment was digested with BamHI and PstI and ligated in pUC18 linearized with the same enzymes. The ligation products were transformed into E. coli TG1 cells, which resulted in 38 recombinant plasmids. Ten of the recombinant plasmids were c ...
... resulted in a product of the expected size of 1?4 kb. This fragment was digested with BamHI and PstI and ligated in pUC18 linearized with the same enzymes. The ligation products were transformed into E. coli TG1 cells, which resulted in 38 recombinant plasmids. Ten of the recombinant plasmids were c ...
REPLI-g WTA Single Cell Handbook
... Different protocols in this handbook provide detailed instructions for using the REPLI-g WTA Single Cell Kit for cDNA amplification of single cells or purified RNA. Use Table 2 (page 15) to select the appropriate protocol based on starting material and downstream analysis. The protocol “Amplificatio ...
... Different protocols in this handbook provide detailed instructions for using the REPLI-g WTA Single Cell Kit for cDNA amplification of single cells or purified RNA. Use Table 2 (page 15) to select the appropriate protocol based on starting material and downstream analysis. The protocol “Amplificatio ...
Diagnostic protocol for
... the disease becomes sporadic as trees reach full fruiting development because when the leaves are not young and the fruits reach their final size, they are not susceptible under natural conditions and fewer angular shoots are produced. Disease severity also depends on the susceptibility of the host ...
... the disease becomes sporadic as trees reach full fruiting development because when the leaves are not young and the fruits reach their final size, they are not susceptible under natural conditions and fewer angular shoots are produced. Disease severity also depends on the susceptibility of the host ...
Expression and V (D) J recombination activity of mutated RAG
... immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination fun ...
... immune system. Little is known of the specific role these genes play. We have explored the sequences encoding mouse RAG-1 by deleting large parts of the gene and by introducing local sequence changes. We find that a RAG-1 gene with 40% of the coding region deleted still retains its recombination fun ...
... The first two bases of the anticodon loop will form “Watson-Crick” –H bonds with the mRNA. The 5’ base of the anticodon can recognize either a U or a C. If it is a G it can form a G-U “wobble” basepair the U, as shown in the upper right. The G can form a normal W-C basepair with the C, as shown in t ...
Lecture 3: Prokaryotic and Eukaryotic Cells
... molecular weight polysaccharides. It is impermeable to the water or other aqueous solvent and it is responsible for antigenicity of bacterial cells. Cell wall in bacteria and its response to gram staining is the basis of classification of bacterial species. WHAT IS GRAM STAINING? Gram staining is de ...
... molecular weight polysaccharides. It is impermeable to the water or other aqueous solvent and it is responsible for antigenicity of bacterial cells. Cell wall in bacteria and its response to gram staining is the basis of classification of bacterial species. WHAT IS GRAM STAINING? Gram staining is de ...
Shedding Light on the Bioluminescence “Paradox”
... is often argued that luciferase has a higher affinity for oxygen than respiration does, and therefore might be useful in decreasing ambient O2. However this is based on assumptions that V. fischeri possesses a low-O2-affinity CyoABCDtype terminal oxidase and on measurements of respiration in cells g ...
... is often argued that luciferase has a higher affinity for oxygen than respiration does, and therefore might be useful in decreasing ambient O2. However this is based on assumptions that V. fischeri possesses a low-O2-affinity CyoABCDtype terminal oxidase and on measurements of respiration in cells g ...
Products > Hep-3B Transfection Reagent
... Altogen Biosystems offers the Hep-3B Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Hep-3B Transfection Reagent is a nanoparticle-based liposome formulation, and it has been developed to provide high transfection efficiency with the Hep-3B cell line. Thi ...
... Altogen Biosystems offers the Hep-3B Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The Hep-3B Transfection Reagent is a nanoparticle-based liposome formulation, and it has been developed to provide high transfection efficiency with the Hep-3B cell line. Thi ...
Cell differentiation during sexual development of the
... per5, which exhibits a sterile phenotype with defects in fruiting body maturation. Cytological investigations revealed that the mutant strain forms only ascus precursors without any mature spores. Using an indexed cosmid library, we were able to complement the mutant to fertility by DNA-mediated tra ...
... per5, which exhibits a sterile phenotype with defects in fruiting body maturation. Cytological investigations revealed that the mutant strain forms only ascus precursors without any mature spores. Using an indexed cosmid library, we were able to complement the mutant to fertility by DNA-mediated tra ...
(THCA) synthase gene in
... # 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: Cannabis sativa; Marijuana; Hemp; THCA synthase gene; DNA polymorphism; Cannabinoid ...
... # 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: Cannabis sativa; Marijuana; Hemp; THCA synthase gene; DNA polymorphism; Cannabinoid ...
RTS™ pIVEX E. coli His-tag 2nd Generation Vector Set Manual
... containing the appropriate restriction sites is prepared, cloning into different pIVEX vectors can be done easily in parallel or successively. To minimize problems, biotechrabbit recommends selecting the cloning strategy strictly according to Table 1. For cloning strategies allowing the minimization ...
... containing the appropriate restriction sites is prepared, cloning into different pIVEX vectors can be done easily in parallel or successively. To minimize problems, biotechrabbit recommends selecting the cloning strategy strictly according to Table 1. For cloning strategies allowing the minimization ...
PPT - Altogen Biosystems
... Altogen Biosystems offers the MG-63 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The MG-63 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfection efficiency with the MG-63 cell line. ...
... Altogen Biosystems offers the MG-63 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The MG-63 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfection efficiency with the MG-63 cell line. ...
Directions for Use Taq DNA Polymerase, 500U
... Can AMRESCO’s UNG replace E. coli UDG in all applications? AMRESCO’s UNG can replace E. coli UDG in all applications except those that require activity above 40°C. Why must dUTP be used in place of dTTP in every amplification reaction? dUTP-containing DNA will be degraded by UNG, but dTTP- containin ...
... Can AMRESCO’s UNG replace E. coli UDG in all applications? AMRESCO’s UNG can replace E. coli UDG in all applications except those that require activity above 40°C. Why must dUTP be used in place of dTTP in every amplification reaction? dUTP-containing DNA will be degraded by UNG, but dTTP- containin ...
1 - CiteSeerX
... This was demonstrated by means of gluconate-l-C 14 in experiments in which the isotope content of the CO2 liberated was found to be 85 to 95 per cent of the isotope in the gluconate fed. Thus, the C1 was converted Io CO2 in the conversion of phosphogluconate to ribulose-5-phosphate, whereas formatio ...
... This was demonstrated by means of gluconate-l-C 14 in experiments in which the isotope content of the CO2 liberated was found to be 85 to 95 per cent of the isotope in the gluconate fed. Thus, the C1 was converted Io CO2 in the conversion of phosphogluconate to ribulose-5-phosphate, whereas formatio ...
Molecular cloning, characterization, and homologous
... effects of pH, temperature, and metal ions on both chitinases produced by recombinant and parental Bt strains were investigated. The present study reports molecular cloning and sequence analysis of an endochitinase gene (chi3023) from Bt serovar morrisoni strain 3023 as well as its homologous expres ...
... effects of pH, temperature, and metal ions on both chitinases produced by recombinant and parental Bt strains were investigated. The present study reports molecular cloning and sequence analysis of an endochitinase gene (chi3023) from Bt serovar morrisoni strain 3023 as well as its homologous expres ...
Diagnostic protocol for
... the disease becomes sporadic as trees reach full fruiting development because when the leaves are not young and the fruits reach their final size, they are not susceptible under natural conditions and fewer angular shoots are produced. Disease severity also depends on the susceptibility of the host ...
... the disease becomes sporadic as trees reach full fruiting development because when the leaves are not young and the fruits reach their final size, they are not susceptible under natural conditions and fewer angular shoots are produced. Disease severity also depends on the susceptibility of the host ...
and Transfer of Ruminococcus pasteurii Schink 1984 to
... ~ out by using previously described methods (15-17,25). Peptidoglycan analysis. Cell walls were prepared and the peptidoglycan structure was determined by the methods of Schleifer and Kandler (28), modified by using thin-layer chromatography on cellulose sheets instead of paper chromatography. Brief ...
... ~ out by using previously described methods (15-17,25). Peptidoglycan analysis. Cell walls were prepared and the peptidoglycan structure was determined by the methods of Schleifer and Kandler (28), modified by using thin-layer chromatography on cellulose sheets instead of paper chromatography. Brief ...
Cell Differentiation during Sexual Development of the Fungus
... per5, which exhibits a sterile phenotype with defects in fruiting body maturation. Cytological investigations revealed that the mutant strain forms only ascus precursors without any mature spores. Using an indexed cosmid library, we were able to complement the mutant to fertility by DNA-mediated tra ...
... per5, which exhibits a sterile phenotype with defects in fruiting body maturation. Cytological investigations revealed that the mutant strain forms only ascus precursors without any mature spores. Using an indexed cosmid library, we were able to complement the mutant to fertility by DNA-mediated tra ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".