Phospholipid synthesis in Borrelia burgdorferi: BB0249 and BB0721
... alignment, the predicted protein for BB0721 has slight homology to Pgs of Bacillus subtilis, Treponema pallidum, Mycoplasma genitalium and Mycoplasma pneumoniae (16 % similarity and 8 % identity). However, BB0721 possesses a conserved CDP-alcohol phosphatidyltransferase motif, DG(X)2AR(X)8G(X)3D(X)3 ...
... alignment, the predicted protein for BB0721 has slight homology to Pgs of Bacillus subtilis, Treponema pallidum, Mycoplasma genitalium and Mycoplasma pneumoniae (16 % similarity and 8 % identity). However, BB0721 possesses a conserved CDP-alcohol phosphatidyltransferase motif, DG(X)2AR(X)8G(X)3D(X)3 ...
8679821 - Southern Illinois University System
... The invention also provides methods of protecting against bacterial contamination. A method of the invention includes adding bactericidal yeast expressing at least one antibacterial protein of the invention to an environment at risk of bacterial contamination. Another method of the invention include ...
... The invention also provides methods of protecting against bacterial contamination. A method of the invention includes adding bactericidal yeast expressing at least one antibacterial protein of the invention to an environment at risk of bacterial contamination. Another method of the invention include ...
Expedient synthesis of 1,2,4-triazolin-3
... wide range of anticancer cells, binding of small molecules with DNA is extremely useful in understanding drug–DNA interactions. Several 2-(2hydroxybenzylideneamino)-isoindoline-1,3-dione transition metal complexes have been reported as DNA cleavage agents23 and pthalidomide (2-(2,6-dioxo-3piperidyl- ...
... wide range of anticancer cells, binding of small molecules with DNA is extremely useful in understanding drug–DNA interactions. Several 2-(2hydroxybenzylideneamino)-isoindoline-1,3-dione transition metal complexes have been reported as DNA cleavage agents23 and pthalidomide (2-(2,6-dioxo-3piperidyl- ...
A systematic search for DNA methyltransferase polymorphisms
... DNMT1 gene at 19p13.2. We screened all 40 exons of the DNMT1 gene. A total number of 25 different single nucleotide polymorphisms (SNPs) and a small fraction of deletions of an ‘AG’ dinucleotide at intron 21 were detected (Supplementary Material, Table S1). Three of these SNPs were found to induce a ...
... DNMT1 gene at 19p13.2. We screened all 40 exons of the DNMT1 gene. A total number of 25 different single nucleotide polymorphisms (SNPs) and a small fraction of deletions of an ‘AG’ dinucleotide at intron 21 were detected (Supplementary Material, Table S1). Three of these SNPs were found to induce a ...
Structural insights into the first incision reaction
... (Sancar, 1994). The strategy employed by NER is the same in all three kingdoms of life. NER in prokaryotes was one of the first repair mechanisms discovered (Boyce and HowardFlanders, 1964; Setlow and Carrier, 1964) and is mediated by the UvrA, UvrB and UvrC proteins. These three proteins recognize a ...
... (Sancar, 1994). The strategy employed by NER is the same in all three kingdoms of life. NER in prokaryotes was one of the first repair mechanisms discovered (Boyce and HowardFlanders, 1964; Setlow and Carrier, 1964) and is mediated by the UvrA, UvrB and UvrC proteins. These three proteins recognize a ...
Structure and expression of the PHO80 gene of Saccharomyces
... PHO80 mRNA levels isolated from cells grown in either high- (H) or low- (L) phosphate-containing growth medium. Total RNA was isolated from strain S288C, fractionated on a 1.5% agarose gel containing 2.2M formaldehyde, transferred to nitrocellulose and hybridized with a p2p]-iabeled 558 bp Xbal-Xbal ...
... PHO80 mRNA levels isolated from cells grown in either high- (H) or low- (L) phosphate-containing growth medium. Total RNA was isolated from strain S288C, fractionated on a 1.5% agarose gel containing 2.2M formaldehyde, transferred to nitrocellulose and hybridized with a p2p]-iabeled 558 bp Xbal-Xbal ...
Structure of DIG
... Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
... Detection by chemiluminescence: a complicated chain of events First: Incorporate “DIG” (Digoxigenin) into your probe DNA Structure of DIG: ...
Lecture 2 - Washington State University
... •Motile by polar flagella; often produce pigments •Most are strict aerobes; no fermentation •Extreme metabolic diversity important in degradation ...
... •Motile by polar flagella; often produce pigments •Most are strict aerobes; no fermentation •Extreme metabolic diversity important in degradation ...
Pfx50™ DNA Polymerase - Thermo Fisher Scientific
... Amplification of longer targets (up to 7 kb) may be possible, but may require more template and longer elongation times. ...
... Amplification of longer targets (up to 7 kb) may be possible, but may require more template and longer elongation times. ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... the bacterial phage pols such as the E. coli phage PRD1 and T4, and the Bacillus phage29. The similarity between these conserved regions is
most striking in their linear spatial arrangement on each pol polypeptide.
The regions are designated I-VI by their extent of similarity, with region
I bein ...
... the bacterial phage pols such as the E. coli phage PRD1 and T4, and the Bacillus phage
PPT - Altogen Biosystems
... Transfection Reagent (Prostate Cancer Cells) Altogen Biosystems offers the PC-3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The PC-3 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transf ...
... Transfection Reagent (Prostate Cancer Cells) Altogen Biosystems offers the PC-3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The PC-3 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transf ...
Molecular and General Genetics
... from 94% to 99%) between these genes, and the sequences were equally close to the thyA sequence from B. subtilis 168 and the thyP3 gene from phage /3T. Slight, but signi®cant dierences between the sequences derived from B. subtilis 168, B. amyloliquefaciens S18 and B. atrophaeus S223 on the one han ...
... from 94% to 99%) between these genes, and the sequences were equally close to the thyA sequence from B. subtilis 168 and the thyP3 gene from phage /3T. Slight, but signi®cant dierences between the sequences derived from B. subtilis 168, B. amyloliquefaciens S18 and B. atrophaeus S223 on the one han ...
Genetics revisited - Institut Montefiore
... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information. DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
... These were originally discovered in 1868 by Friedrich Meischer (isolating DNA from pus cells on bandages). At that time, he could not confirm that nucleic acids might contain genetic information. DNA IS the genetic information of most living organisms. In contrast, some viruses (called retroviruse ...
CHaPter 2 Nucleic acids and proteins: a review
... Table 2.1 Part of the sequences of different genes from various organisms. Numbers are placed above the sequences for ease of locating a particular nucleotide. ...
... Table 2.1 Part of the sequences of different genes from various organisms. Numbers are placed above the sequences for ease of locating a particular nucleotide. ...
Cloning and characterization of CmGPD1, the Candida magnoliae
... yeast as compatible solutes. When yeast cells are exposed to hyperosmotic stress, they accumulate one or more protective solutes such as glycerol, D-arabitol and mannitol. These polyols prevent the rapid diffusion of water from the cell into the surrounding medium to compensate for the loss of turgo ...
... yeast as compatible solutes. When yeast cells are exposed to hyperosmotic stress, they accumulate one or more protective solutes such as glycerol, D-arabitol and mannitol. These polyols prevent the rapid diffusion of water from the cell into the surrounding medium to compensate for the loss of turgo ...
Vibrio diabolicus sp. nov., a New Polysaccharide
... Physiological characteristics. Growth experiments were performed in 20-ml tubes containing 5 ml of marine 2216 broth. The tubes were inoculated (5%, vol/vol) with a primary bacterial culture in the exponential phase and were incubated inclined on a table rotary shaker (Infors HT, Bottmingen, Switzer ...
... Physiological characteristics. Growth experiments were performed in 20-ml tubes containing 5 ml of marine 2216 broth. The tubes were inoculated (5%, vol/vol) with a primary bacterial culture in the exponential phase and were incubated inclined on a table rotary shaker (Infors HT, Bottmingen, Switzer ...
This article was published in an Elsevier journal. The attached copy
... mammalian cell to create the full-length viral genome. Additionally, VZV has a narrow host range and is highly cell-associated in vitro, which makes it more complicated to be mutagenized and isolated than other alpha herpesviruses (Cohen et al., 2007). To solve this problem, the full-length VZV (P-O ...
... mammalian cell to create the full-length viral genome. Additionally, VZV has a narrow host range and is highly cell-associated in vitro, which makes it more complicated to be mutagenized and isolated than other alpha herpesviruses (Cohen et al., 2007). To solve this problem, the full-length VZV (P-O ...
Caloranaerobacter ferrireducens sp. nov., an anaerobic
... acid profiles of strain DY22619T and Caloranaerobacter azorensis MV1087T are given in Table S1. The G+C content of the chromosomal DNA was determined according to the methods described using reverse-phase HPLC (Mesbah & Whitman, 1989). The DNA G+C content of the novel isolate DY22619T was 29.0 mol%, ...
... acid profiles of strain DY22619T and Caloranaerobacter azorensis MV1087T are given in Table S1. The G+C content of the chromosomal DNA was determined according to the methods described using reverse-phase HPLC (Mesbah & Whitman, 1989). The DNA G+C content of the novel isolate DY22619T was 29.0 mol%, ...
A. Introduction
... There is a balance between the rate at which species already present become locally extinct. The number of species is an equilibrium between those two rates. The composition of the species is in non-equilibrium. When a species becomes extinct, it may be replaced by a different immigrating species. T ...
... There is a balance between the rate at which species already present become locally extinct. The number of species is an equilibrium between those two rates. The composition of the species is in non-equilibrium. When a species becomes extinct, it may be replaced by a different immigrating species. T ...
ADVANCING JUSTICE THROUGH DNA TECHNOLOGY
... was required to provide a sample of his DNA, and the resulting DNA profile was entered into a DNA database. Several years later, another sexual assault was committed. A Sexual Assault Nurse Examiner worked with the victim and was able to obtain biological evidence from the rape. This evidence was an ...
... was required to provide a sample of his DNA, and the resulting DNA profile was entered into a DNA database. Several years later, another sexual assault was committed. A Sexual Assault Nurse Examiner worked with the victim and was able to obtain biological evidence from the rape. This evidence was an ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".