Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
... containing 20 units of T4 RNA Ligase 1 with 40 ng of RNA transcript for 2 hours at 37°C resulted in no detectable degradation of the RNA as determined by gel electrophoresis. DNA Exonuclease Activity: Incubation of 20 units of T4 RNA Ligase 1 with 1 µg of mixed single and double-stranded sonicated 3 ...
... containing 20 units of T4 RNA Ligase 1 with 40 ng of RNA transcript for 2 hours at 37°C resulted in no detectable degradation of the RNA as determined by gel electrophoresis. DNA Exonuclease Activity: Incubation of 20 units of T4 RNA Ligase 1 with 1 µg of mixed single and double-stranded sonicated 3 ...
Incorporation of reporter molecule
... modi®ed dNTP combinations identi®ed and con®rmed with these two model template assay systems were then tested further in the natural DNA template assay with a pUC19-derived template (see Materials and Methods). Evaluation of modi®ed dNTP substrates First, the substrate properties of each modi®ed dNT ...
... modi®ed dNTP combinations identi®ed and con®rmed with these two model template assay systems were then tested further in the natural DNA template assay with a pUC19-derived template (see Materials and Methods). Evaluation of modi®ed dNTP substrates First, the substrate properties of each modi®ed dNT ...
Electron transfer from aromatic amino acids to guanine and adenine
... Because in most complexes G–X, DE is positive and the coupling is relatively small, the excess charge is mainly confined to G. Only in the edge dimers with X = Trp, Tyr and His, where DE < 0, the radical cation state is found on X. Since in the stacked dimers G–Trp, absolute values of DE and V are si ...
... Because in most complexes G–X, DE is positive and the coupling is relatively small, the excess charge is mainly confined to G. Only in the edge dimers with X = Trp, Tyr and His, where DE < 0, the radical cation state is found on X. Since in the stacked dimers G–Trp, absolute values of DE and V are si ...
Chapter 6 Pichia pastoris
... Cytochrome P450s are one of the largest superfamilies of heme-containing enzymes (Chapple, 1998) found in almost all living organisms. Up to now P450s have been found in bacteria, insects, fish, mammals, plants, and fungi (Chapple, 1998). Cinnamic acid 4hydroxylase (C4H) is a cytochrome P450-depende ...
... Cytochrome P450s are one of the largest superfamilies of heme-containing enzymes (Chapple, 1998) found in almost all living organisms. Up to now P450s have been found in bacteria, insects, fish, mammals, plants, and fungi (Chapple, 1998). Cinnamic acid 4hydroxylase (C4H) is a cytochrome P450-depende ...
Alu electrophoresis PCR lab
... Agarose is a polysaccharide (carbohydrate) polymer material, generally extracted from seaweed. ...
... Agarose is a polysaccharide (carbohydrate) polymer material, generally extracted from seaweed. ...
DNA and RNA Extraction Controls Performance Summary
... 0.5mM EDTA 1mM EDTA 2mM EDTA 2.5mM EDTA 3mM EDTA 3.5mM EDTA 4mM EDTA ...
... 0.5mM EDTA 1mM EDTA 2mM EDTA 2.5mM EDTA 3mM EDTA 3.5mM EDTA 4mM EDTA ...
Week Date Hours Topic Standard IBO Assessment Statement Labs
... these tissues. Identify modifications of roots, stems and leaves for different functions: bulbs, stem tubers, storage roots and tendrils. State that dicots have apical and lateral meristems. ...
... these tissues. Identify modifications of roots, stems and leaves for different functions: bulbs, stem tubers, storage roots and tendrils. State that dicots have apical and lateral meristems. ...
Carcinoembryonic Antigens - The Journal of Cell Biology
... expressed by a number of tumor cell lines and by normal fetal liver. Despite their extensive amino acid sequence similarities, the four protein species differ from CEA and NCA in that they contain transmembrane and cytoplasmic domains. For this reason we have designated these molecules transmembrane ...
... expressed by a number of tumor cell lines and by normal fetal liver. Despite their extensive amino acid sequence similarities, the four protein species differ from CEA and NCA in that they contain transmembrane and cytoplasmic domains. For this reason we have designated these molecules transmembrane ...
The methylcitric acid pathway in Ralstonia eutropha
... complementation of a prpC mutant of Salmonella enterica serovar Typhimurium and high sequence similarity. (ii) For the translational product of acnM the function of a 2-methyl-cis-aconitic acid hydratase (94 726 Da) is proposed. This protein and also the ORF5 translational product are essential for ...
... complementation of a prpC mutant of Salmonella enterica serovar Typhimurium and high sequence similarity. (ii) For the translational product of acnM the function of a 2-methyl-cis-aconitic acid hydratase (94 726 Da) is proposed. This protein and also the ORF5 translational product are essential for ...
UNIT – I: NUCLEIC ACID AND PROTEIN SYNTHESIS AND
... base, uracil , which can also base pair with adenine In addition, RNA molecules normally exist as a single polynucletide strand and do not form a double helix. However, it is possible for base pairing to occur between complementary parts of the same RNA strand resulting in short double stranded regi ...
... base, uracil , which can also base pair with adenine In addition, RNA molecules normally exist as a single polynucletide strand and do not form a double helix. However, it is possible for base pairing to occur between complementary parts of the same RNA strand resulting in short double stranded regi ...
Development of an internally controlled real-time PCR
... (c. 30% (8 ⁄ 27) of the annual reported cases in The Netherlands) [3]. One of these samples had been shown previously to be PCR-positive with a different primer set [8,11], and six cases were confirmed serologically (the two remaining cases showed increasing titres only of specific IgG). In general, ...
... (c. 30% (8 ⁄ 27) of the annual reported cases in The Netherlands) [3]. One of these samples had been shown previously to be PCR-positive with a different primer set [8,11], and six cases were confirmed serologically (the two remaining cases showed increasing titres only of specific IgG). In general, ...
Diagnostic protocol for
... the bacterial multiplication and development of incipient pustules of the disease. Once inoculated with macerated lesions, it constitutes a very sensitive (detect 102 cfu/ml) and specific diagnosis method. The procedure for the application of this bioassay begin by sterilizing ELISA plates for 15 mi ...
... the bacterial multiplication and development of incipient pustules of the disease. Once inoculated with macerated lesions, it constitutes a very sensitive (detect 102 cfu/ml) and specific diagnosis method. The procedure for the application of this bioassay begin by sterilizing ELISA plates for 15 mi ...
The Family of SMF Metal Ion Transporters in Yeast Cells*
... Genes—The main feature of the SMF1 null mutant was its sensitivity to EGTA concentrations, toward which the parental wild-type strain was insensitive (8). The growth inhibition of ⌬SMF1 in the presence of EGTA could be alleviated by low concentrations of manganese or copper but not by any other meta ...
... Genes—The main feature of the SMF1 null mutant was its sensitivity to EGTA concentrations, toward which the parental wild-type strain was insensitive (8). The growth inhibition of ⌬SMF1 in the presence of EGTA could be alleviated by low concentrations of manganese or copper but not by any other meta ...
Metabolic Enzymes
... – Temperate phages (or lysogenic phages) inject their DNA into a bacterial cell. – The phage DNA integrates into the bacterial chromosome but does not cause the lytic cycle to occur. This is known as lysogeny. – A phage is called a prophage when all that remains of it is its DNA. – The bacterial cel ...
... – Temperate phages (or lysogenic phages) inject their DNA into a bacterial cell. – The phage DNA integrates into the bacterial chromosome but does not cause the lytic cycle to occur. This is known as lysogeny. – A phage is called a prophage when all that remains of it is its DNA. – The bacterial cel ...
Quantitative parameters for amino acid–base
... The continuously increasing number of crystallographically solved protein–DNA complexes challenges the derivation of a quantitative measure for all possible amino acid–base interactions from their frequencies in the three-dimensional structures of the complexes, similarly to the extraction of knowle ...
... The continuously increasing number of crystallographically solved protein–DNA complexes challenges the derivation of a quantitative measure for all possible amino acid–base interactions from their frequencies in the three-dimensional structures of the complexes, similarly to the extraction of knowle ...
DNA Base Composition, DNA-DNA Homology and Long
... 1983). Streptococcus mitis, S. oralis and S . sanguis differed from S.salivarius and S . thermophilus in lacking substantial levels (about 12 to 17%) of C20:lfatty acids (Table 3). The presence of closely related fatty acid profiles within S. salivarius and S . therrnophilus further supports the clo ...
... 1983). Streptococcus mitis, S. oralis and S . sanguis differed from S.salivarius and S . thermophilus in lacking substantial levels (about 12 to 17%) of C20:lfatty acids (Table 3). The presence of closely related fatty acid profiles within S. salivarius and S . therrnophilus further supports the clo ...
DNA Base Composition, DNA-DNA Homology and Long
... 1983). Streptococcus mitis, S. oralis and S . sanguis differed from S.salivarius and S . thermophilus in lacking substantial levels (about 12 to 17%) of C20:lfatty acids (Table 3). The presence of closely related fatty acid profiles within S. salivarius and S . therrnophilus further supports the clo ...
... 1983). Streptococcus mitis, S. oralis and S . sanguis differed from S.salivarius and S . thermophilus in lacking substantial levels (about 12 to 17%) of C20:lfatty acids (Table 3). The presence of closely related fatty acid profiles within S. salivarius and S . therrnophilus further supports the clo ...
Lower Respiratory Tract Infections
... Special media for growth amino acids L-cysteine Ferric ions Cx on buffered media (pH restrictions) SLOW growth (2-5d) ...
... Special media for growth amino acids L-cysteine Ferric ions Cx on buffered media (pH restrictions) SLOW growth (2-5d) ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".