One-stop polymerase chain reaction (PCR): An improved PCR
... Figure 2. Efficiency and effect of one-stop PCR on nucleic acid amplification. (A) One-stop PCR is comparable to commercially available PCR kits. A 0.4 kb fragment of human β-actin mRNA was amplified with gradient template amount at dilutions of 1/1, 1/10, 1/100. All the PCR products were separated ...
... Figure 2. Efficiency and effect of one-stop PCR on nucleic acid amplification. (A) One-stop PCR is comparable to commercially available PCR kits. A 0.4 kb fragment of human β-actin mRNA was amplified with gradient template amount at dilutions of 1/1, 1/10, 1/100. All the PCR products were separated ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... The GMO Investigator kit uses multiplex PCR in the GMO reactions, while the plant primer reactions are singleplex. Please note that the “multiplex” in the GMO kit is slightly different than the “multiplexing” described above. In the GMO Investigator kit, primer pairs are mixed (multiplexed) together ...
... The GMO Investigator kit uses multiplex PCR in the GMO reactions, while the plant primer reactions are singleplex. Please note that the “multiplex” in the GMO kit is slightly different than the “multiplexing” described above. In the GMO Investigator kit, primer pairs are mixed (multiplexed) together ...
TRIzol Reagent
... Chomczynski, P. and Mackey, K. (1995) Anal. Biochem. 225, 163-164). The quantity and quality of RNA is the same with both reagents. The amount of BCP used for phase separation equals 10% of the TRIzol volume. Using lower centrifugation speeds: Centrifugation speeds as low as 5000 -6000 x g have been ...
... Chomczynski, P. and Mackey, K. (1995) Anal. Biochem. 225, 163-164). The quantity and quality of RNA is the same with both reagents. The amount of BCP used for phase separation equals 10% of the TRIzol volume. Using lower centrifugation speeds: Centrifugation speeds as low as 5000 -6000 x g have been ...
Microplate-Based Pathlength Correction Method for Photometric
... correction is performed, the results are no longer dependent on the volume. The microplate results—after pathlength correction—correlate perfectly with the cuvette results. The only exception seen in the figures is a slight variation between the 20 µl assay volume results using 384-well plates and t ...
... correction is performed, the results are no longer dependent on the volume. The microplate results—after pathlength correction—correlate perfectly with the cuvette results. The only exception seen in the figures is a slight variation between the 20 µl assay volume results using 384-well plates and t ...
Identiflcation and typing of grapevine phytoplasma amplified by graft
... causative agent of the grapevine yellows disease flavescence doree is transmitted by Scaphoideus titanus (CAUDWELL et a[. 1971; MEIGNOZ et al. 1992) and in Germany the Vergilbungskrankheit is transmitted by Hyalesthes obsoletus (MAIXNER et al. 1995). Many plants are affected by phytoplasma and many ...
... causative agent of the grapevine yellows disease flavescence doree is transmitted by Scaphoideus titanus (CAUDWELL et a[. 1971; MEIGNOZ et al. 1992) and in Germany the Vergilbungskrankheit is transmitted by Hyalesthes obsoletus (MAIXNER et al. 1995). Many plants are affected by phytoplasma and many ...
unit 2: mechanisms of inheritance
... B12-0-N1: Describe the role of evidence in developing scientific understanding and explain how this understanding changes when new evidence is introduced. (GLO: A2) B12-0-N2: understand that development and acceptance of scientific evidence, theories, or technologies are affected by many factors. (G ...
... B12-0-N1: Describe the role of evidence in developing scientific understanding and explain how this understanding changes when new evidence is introduced. (GLO: A2) B12-0-N2: understand that development and acceptance of scientific evidence, theories, or technologies are affected by many factors. (G ...
Instructor`s Guide
... Students will determine the genotype of SNP785 of their own TAS2R38 genes, in which a cytosine to thymine change at the nucleotide level results in an alanine to valine change at the amino acid level. At the same time students will test their ability to taste the bitter compound PTC and correlate it ...
... Students will determine the genotype of SNP785 of their own TAS2R38 genes, in which a cytosine to thymine change at the nucleotide level results in an alanine to valine change at the amino acid level. At the same time students will test their ability to taste the bitter compound PTC and correlate it ...
DNA sentences How are proteins coded for by DNA?
... words of the sentence and write that sentence in large print/script on the transcription/translation data sheet. Extension: After groups have translated sentences, research each statement to find evidence to support or refute. Hint: The first triplet code is a “start” code, which in eukaryotes, repr ...
... words of the sentence and write that sentence in large print/script on the transcription/translation data sheet. Extension: After groups have translated sentences, research each statement to find evidence to support or refute. Hint: The first triplet code is a “start” code, which in eukaryotes, repr ...
Novel Research Starts with GAPDH - Bio-Rad
... Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School and Delta College, Tracy, CA Bio-Rad Curriculum and Training Specialists: Sherri Andrews, Ph.D. [email protected] Essy Levy, M.Sc. [email protected] Leigh Brown, M.A. [email protected] ...
... Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School and Delta College, Tracy, CA Bio-Rad Curriculum and Training Specialists: Sherri Andrews, Ph.D. [email protected] Essy Levy, M.Sc. [email protected] Leigh Brown, M.A. [email protected] ...
J Urol - prostata
... PURPOSE: DNA ploidy has consistently been found to be a correlate of prostate cancer patient outcome. However, a minority of studies have used pretreatment diagnostic material and have involved radiotherapy (RT)-treated patients. In this retrospective study, the predictive value of DNA ploidy was ev ...
... PURPOSE: DNA ploidy has consistently been found to be a correlate of prostate cancer patient outcome. However, a minority of studies have used pretreatment diagnostic material and have involved radiotherapy (RT)-treated patients. In this retrospective study, the predictive value of DNA ploidy was ev ...
Vitis 37 (3), 119
... The group of "Muscat grapes" ("Moschoudia" in Greece) includes some very interesting cultivars grown all over Europe. The most important are Muscat de Frontignan, Moschato aspro (production of dessert wines), Muscat of Alexandria (a multipurpose variety) and Muscat Hamburg (a table grape variety). T ...
... The group of "Muscat grapes" ("Moschoudia" in Greece) includes some very interesting cultivars grown all over Europe. The most important are Muscat de Frontignan, Moschato aspro (production of dessert wines), Muscat of Alexandria (a multipurpose variety) and Muscat Hamburg (a table grape variety). T ...
The Polymerase Chain Reaction
... Uses retroviral enzyme reverse transcriptase to reverse transcribe mRNA into DNA before the PCR begins When DNA is made this way it is referred to as cDNA – RT-PCR is often used in combination with real-time PCR to measure levels of gene expression Reverse transcription ...
... Uses retroviral enzyme reverse transcriptase to reverse transcribe mRNA into DNA before the PCR begins When DNA is made this way it is referred to as cDNA – RT-PCR is often used in combination with real-time PCR to measure levels of gene expression Reverse transcription ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""