Gel Electrophoresis
... Agarose is a polysaccharide obtained from red seaweed. The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relativ ...
... Agarose is a polysaccharide obtained from red seaweed. The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relativ ...
e Study of RNA Polymerase Pausing by Optical Traps
... experiment because one RNAP transcribes identical pause sequences multiple times. We tracked the movements of individual Escherichia coli RNAPs with an optical trap, a device that utilizes a highly focused infrared laser to manipulate single molecules. Optical trapping experiments give accurate meas ...
... experiment because one RNAP transcribes identical pause sequences multiple times. We tracked the movements of individual Escherichia coli RNAPs with an optical trap, a device that utilizes a highly focused infrared laser to manipulate single molecules. Optical trapping experiments give accurate meas ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... Substantial effort has been spent in pushing the resolution of optical tweezers to allow one of the most elementary events in molecular biology, the stepping of a polymerase enzyme over a single base pair, to be resolved in real time. However, what are the ultimate limits of optical tweezers and wha ...
... Substantial effort has been spent in pushing the resolution of optical tweezers to allow one of the most elementary events in molecular biology, the stepping of a polymerase enzyme over a single base pair, to be resolved in real time. However, what are the ultimate limits of optical tweezers and wha ...
Southern molecular hybridization experiments with parallel
... The previously cloned sequences of A282 and p8.3, containing the same 8.3 kb EcoRl fragment from the cut locus of D. melanogaster [14] were used as the model for studying the hybridization of Southern filters with the parallel complementary probe (probe A). The same blot was hybridized in 2x SSC sol ...
... The previously cloned sequences of A282 and p8.3, containing the same 8.3 kb EcoRl fragment from the cut locus of D. melanogaster [14] were used as the model for studying the hybridization of Southern filters with the parallel complementary probe (probe A). The same blot was hybridized in 2x SSC sol ...
Strategies in the interfield discovery of the mechanism of protein
... bonds. Zamecnik and his colleagues, especially Mahlon Hoagland, sought to understand energetic intermediates between free amino acids and their linkage in polypeptides (recalled in Zamecnik, 1962a, 1969; Hoagland, 1990, 1996). They were thus working backward from peptide bonds to the mechanisms of p ...
... bonds. Zamecnik and his colleagues, especially Mahlon Hoagland, sought to understand energetic intermediates between free amino acids and their linkage in polypeptides (recalled in Zamecnik, 1962a, 1969; Hoagland, 1990, 1996). They were thus working backward from peptide bonds to the mechanisms of p ...
EPICENTRE Enzyme Catalog
... manufactures, and sells high-quality enzyme systems for life science research. Located in Madison, Wisconsin, EPICENTRE was founded in 1987, and now occupies a state-of-the art 72,000-s.f. building. EPICENTRE is well-known for its unique expertise in making a broad range of enzymes for molecular bio ...
... manufactures, and sells high-quality enzyme systems for life science research. Located in Madison, Wisconsin, EPICENTRE was founded in 1987, and now occupies a state-of-the art 72,000-s.f. building. EPICENTRE is well-known for its unique expertise in making a broad range of enzymes for molecular bio ...
DNAse I Qualification and Sample Treatment | Molecular Devices
... The Threshold Total DNA Assay quantitates contaminant DNA in biopharmaceutical drugs. DNase I can be used in conjunction with the Total DNA Assay for two purposes: To validate that the assay signal and subsequent quantitation generated by the Total DNA Assay specifically measures DNA and not a non-DN ...
... The Threshold Total DNA Assay quantitates contaminant DNA in biopharmaceutical drugs. DNase I can be used in conjunction with the Total DNA Assay for two purposes: To validate that the assay signal and subsequent quantitation generated by the Total DNA Assay specifically measures DNA and not a non-DN ...
De Bruijn Graphs for DNA Sequencing (Part 1)
... that are complementary to substrings of length k of the fragment. Modelli Biologici Discreti ...
... that are complementary to substrings of length k of the fragment. Modelli Biologici Discreti ...
GF-1 Food DNA Extraction Kit
... origins. This kit uses a specially-treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. The kit applies the principle of a minicolumn spin technology and the use of optimized buffers to ensure that only DNA is isolated while proteins and other impur ...
... origins. This kit uses a specially-treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. The kit applies the principle of a minicolumn spin technology and the use of optimized buffers to ensure that only DNA is isolated while proteins and other impur ...
Classification of nucleic acids structures by means of the
... Since the initial elucidation of the secondary structure of B-DNA duplex by Watson and Crick in 1953 [1], additional DNA secondary structures have been described in the literature (Scheme 1) [2, 3]. These are related to base and sugar geometries different from those found in the B-DNA duplex structu ...
... Since the initial elucidation of the secondary structure of B-DNA duplex by Watson and Crick in 1953 [1], additional DNA secondary structures have been described in the literature (Scheme 1) [2, 3]. These are related to base and sugar geometries different from those found in the B-DNA duplex structu ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""