A Mathematical Formulation of DNA Computation
... detected. In conventional terminologies of computing, the DNA strands may be regarded as hardware. Strand coding can be treated as software. The operating system is to read DNA strands through bio-molecular techniques. DNA computation is attractive mainly for three reasons. First, the computation re ...
... detected. In conventional terminologies of computing, the DNA strands may be regarded as hardware. Strand coding can be treated as software. The operating system is to read DNA strands through bio-molecular techniques. DNA computation is attractive mainly for three reasons. First, the computation re ...
DNA Sequencing
... Identifying Clones Carrying a Gene of Interest • A clone carrying the gene of interest – Can be identified with a radioactively labeled nucleic acid probe that has a sequence complementary to the gene, a process called ...
... Identifying Clones Carrying a Gene of Interest • A clone carrying the gene of interest – Can be identified with a radioactively labeled nucleic acid probe that has a sequence complementary to the gene, a process called ...
III. MATERIAL AND METHODS The present study was undertaken
... Taqpolymerase enzyme (Genei, India), two concentrations each of DNA (5, 10 ng) and dNTP (0.1, 0.2 mM, Eppendorf, USA) were varied in different combinations and the combination that gave good amplification was selected and used for further experiments. Amplifications were performed in a final volume ...
... Taqpolymerase enzyme (Genei, India), two concentrations each of DNA (5, 10 ng) and dNTP (0.1, 0.2 mM, Eppendorf, USA) were varied in different combinations and the combination that gave good amplification was selected and used for further experiments. Amplifications were performed in a final volume ...
Nucleic Acids
... site of primary genetic activity within cells. In prokaryotic cells (i.e., cells lacking a nucleus) genetic activity occurs throughout the cytoplasm. Thus, the various molecules of circular DNA (chromosome and plasmids) residing in prokaryotic cells are not localized to a specific compartment of the ...
... site of primary genetic activity within cells. In prokaryotic cells (i.e., cells lacking a nucleus) genetic activity occurs throughout the cytoplasm. Thus, the various molecules of circular DNA (chromosome and plasmids) residing in prokaryotic cells are not localized to a specific compartment of the ...
Measuring Double-Stranded DNA Concentration Using the Quantus
... • Qubit® dsDNA BR Assay Kit (Life Technologies Cat.# Q32850) Caution: We recommend the use of gloves, lab coats and eye protection when working with these or any chemical reagents. Protocol: Quantus™ Fluorometer Operating Manual #TM396 is available at: ...
... • Qubit® dsDNA BR Assay Kit (Life Technologies Cat.# Q32850) Caution: We recommend the use of gloves, lab coats and eye protection when working with these or any chemical reagents. Protocol: Quantus™ Fluorometer Operating Manual #TM396 is available at: ...
1. Introduction - diss.fu
... sites on chromosomes or plasmids. They are distributed across the living world, and play a fundamental role as motors of genome plasticity. Transposons were first discovered in maize (=HD PD\V) by Barbara McClintock in the 1940s (McClintock, 1987). McClintock was studying the genetic consequences o ...
... sites on chromosomes or plasmids. They are distributed across the living world, and play a fundamental role as motors of genome plasticity. Transposons were first discovered in maize (=HD PD\V) by Barbara McClintock in the 1940s (McClintock, 1987). McClintock was studying the genetic consequences o ...
Table of Contents
... extend long templates in a fraction of the time, making Phusion a superior choice for cloning. Phusion is suitable for all PCR applications requiring greater accuracy or long amplicons. 3. Have the formulations or any other characteristics of these products changed now that they are manufactured by ...
... extend long templates in a fraction of the time, making Phusion a superior choice for cloning. Phusion is suitable for all PCR applications requiring greater accuracy or long amplicons. 3. Have the formulations or any other characteristics of these products changed now that they are manufactured by ...
Bacterial Screening PCR Kit
... 4) Use the centrifuged supernatant as the DNA Sample Solution for PCR. [Option 2] (Use of 1.5 ml micro test tube with screw cap is recommended.) 1) Suspend the pellet containing the centrifuged bacteria (see step 2 in section C above) in 100 μ l of acromopeptidase*** (250 U/ml, in TE buffer) and inc ...
... 4) Use the centrifuged supernatant as the DNA Sample Solution for PCR. [Option 2] (Use of 1.5 ml micro test tube with screw cap is recommended.) 1) Suspend the pellet containing the centrifuged bacteria (see step 2 in section C above) in 100 μ l of acromopeptidase*** (250 U/ml, in TE buffer) and inc ...
- Peanut Science
... samples were loaded as for agarose gels. Care was taken to avoid overexposure of the gel to air to prevent shrinkage. Five ml of Type IV gel loading buffer described by Sambrook et al. (1989) were added to the 10 ml PCR product, and 4 ml of the sample were loaded on the gel. Gels were run in a subma ...
... samples were loaded as for agarose gels. Care was taken to avoid overexposure of the gel to air to prevent shrinkage. Five ml of Type IV gel loading buffer described by Sambrook et al. (1989) were added to the 10 ml PCR product, and 4 ml of the sample were loaded on the gel. Gels were run in a subma ...
DNA Sequencing
... Identifying Clones Carrying a Gene of Interest • A clone carrying the gene of interest – Can be identified with a radioactively labeled nucleic acid probe that has a sequence complementary to the gene, a process called ...
... Identifying Clones Carrying a Gene of Interest • A clone carrying the gene of interest – Can be identified with a radioactively labeled nucleic acid probe that has a sequence complementary to the gene, a process called ...
Automated genomic DNA purification of 6 different marine
... invertebrate samples can easily be purified with the MACHEREY‐NAGEL NucleoSpin® 8/96 Tissue kit and the automated epMotion® 5075 VAC system. The method delivers consistently high purity DNA with an average A260/280 ratio of 1.83, indicating low protein contamination. The average yield across the sam ...
... invertebrate samples can easily be purified with the MACHEREY‐NAGEL NucleoSpin® 8/96 Tissue kit and the automated epMotion® 5075 VAC system. The method delivers consistently high purity DNA with an average A260/280 ratio of 1.83, indicating low protein contamination. The average yield across the sam ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""