GeneMATRIX Universal DNA/RNA/Protein Purification Kit
... 3. Carefully transfer the supernatant to the DNA binding spin-column placed in a 2 ml collection tube. Centrifuge at maximum speed for 1 minute. 4. Store the DNA binding spin-column at room temperature 15÷25 oC or at 2÷8oC for later DNA purification (part IV of the protocol). Use the flow-through fo ...
... 3. Carefully transfer the supernatant to the DNA binding spin-column placed in a 2 ml collection tube. Centrifuge at maximum speed for 1 minute. 4. Store the DNA binding spin-column at room temperature 15÷25 oC or at 2÷8oC for later DNA purification (part IV of the protocol). Use the flow-through fo ...
RECOMBINANT-DNA METHODOLOGY
... Most useful restriction enzymes cut DNA at specific recognition sites, usually four to six nucleotides in length. There can be multiple restriction sites for a single endonuclease within a given piece of DNA, there can be only one (a unique restriction site), or there can be none. It all depends on ...
... Most useful restriction enzymes cut DNA at specific recognition sites, usually four to six nucleotides in length. There can be multiple restriction sites for a single endonuclease within a given piece of DNA, there can be only one (a unique restriction site), or there can be none. It all depends on ...
Protocol for RiboShredder™ RNase Blend
... of applications. When used for DNA purification, all unwanted RNA can be removed using a simple 10-minute procedure. After the reaction is complete, RiboShredder RNase Blend can be removed using a phenol-chloroform extraction procedure. It’s broad range of salt tolerance makes it ideal for use in ma ...
... of applications. When used for DNA purification, all unwanted RNA can be removed using a simple 10-minute procedure. After the reaction is complete, RiboShredder RNase Blend can be removed using a phenol-chloroform extraction procedure. It’s broad range of salt tolerance makes it ideal for use in ma ...
The effect of human serum DNAases on the ability to detect
... animals [2]. Nevertheless, it is still uncertain just how reliable PCR is and whether a negative result truly re¯ects the absence of bacteria in blood [4, 5], as mechanisms reducing the availability of the bacterial template DNA may cause false-negative PCR results. When bacteria are killed by antib ...
... animals [2]. Nevertheless, it is still uncertain just how reliable PCR is and whether a negative result truly re¯ects the absence of bacteria in blood [4, 5], as mechanisms reducing the availability of the bacterial template DNA may cause false-negative PCR results. When bacteria are killed by antib ...
MagJET Plasmid DNA Kit - Thermo Fisher Scientific
... ready for use in downstream applications or can be stored at -20°C for later use. ...
... ready for use in downstream applications or can be stored at -20°C for later use. ...
Single-stranded DNA-binding Proteins
... The combination of electrostatic, hydrogen-bonding and stacking interactions from proteins to ssDNA forms the basis for ssDNA binding and specificity. Unfortunately, dsDNA, dsRNA and single-stranded RNA (ssRNA) share many of these properties. How does an ssDNA-binding protein exclude these competing ...
... The combination of electrostatic, hydrogen-bonding and stacking interactions from proteins to ssDNA forms the basis for ssDNA binding and specificity. Unfortunately, dsDNA, dsRNA and single-stranded RNA (ssRNA) share many of these properties. How does an ssDNA-binding protein exclude these competing ...
DNA purification and isolation of genomic DNA from bacterial
... *Corresponding author. E-mail: [email protected]. ...
... *Corresponding author. E-mail: [email protected]. ...
Molecular and Immunological Methods
... fluorophore, and a 3’ fluorophore (e.g. TAMRA) or non-fluorescent quencher (NFQ). Energy generated by the excitation of the 5’ fluorophore is captured by the 3’ quencher, and emitted as fluorescence or heat (NFQ). If a second fluorophore is the quencher, the emission wavelength is different to that ...
... fluorophore, and a 3’ fluorophore (e.g. TAMRA) or non-fluorescent quencher (NFQ). Energy generated by the excitation of the 5’ fluorophore is captured by the 3’ quencher, and emitted as fluorescence or heat (NFQ). If a second fluorophore is the quencher, the emission wavelength is different to that ...
Glencoe Biology
... English Shorthorn cattle, which provide good beef but cannot withstand hot environments, and Brahman cattle from India that have a high heat tolerance but produce ...
... English Shorthorn cattle, which provide good beef but cannot withstand hot environments, and Brahman cattle from India that have a high heat tolerance but produce ...
dna replication
... Before the reader can gain an understanding of the practical applications of nucleic acids and proteins, the basic structure, mode of action and functions of these complex structures should be known. Two types of nucleic acids can be found in a eukaryotic cell; deoxyribonucleic acid (DNA) and ribonu ...
... Before the reader can gain an understanding of the practical applications of nucleic acids and proteins, the basic structure, mode of action and functions of these complex structures should be known. Two types of nucleic acids can be found in a eukaryotic cell; deoxyribonucleic acid (DNA) and ribonu ...
K-3034-2 96 well PCR Puri kit - +¦¦«++-+ 041001
... according to needs. Using less than 80 ul is optionally possible, however, DNA yield could be reduced due to the decreased volume. It will not cause any trouble to leave them longer or to use warm EL buffer, which is boiled up to 60°C or 140°F. Over 7 0% DNA will be obtained through following this ...
... according to needs. Using less than 80 ul is optionally possible, however, DNA yield could be reduced due to the decreased volume. It will not cause any trouble to leave them longer or to use warm EL buffer, which is boiled up to 60°C or 140°F. Over 7 0% DNA will be obtained through following this ...
Compiling DNA strand displacement reactions using a functional
... by parallel lines, with arrowheads denoting strand orientations. Instead of dealing with nucleotide sequences (G, A, T , C) we define domains as shorthands for particular finite sequences. We write x∗ for the complement of the domain x, which is the domain that binds to x. This is defined by the sta ...
... by parallel lines, with arrowheads denoting strand orientations. Instead of dealing with nucleotide sequences (G, A, T , C) we define domains as shorthands for particular finite sequences. We write x∗ for the complement of the domain x, which is the domain that binds to x. This is defined by the sta ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""