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... kindly supplied by Dr. Sasson Cohen and Dr. Aaron Bendich. All other haptens used were commercial products of standard purity. Immunoehemical l~rocedures.--Rabbits were injected once weekly for 3 weeks into the foot-pads (10) with Pur-BSA or Pur-HSA in complete Freund's adjuvant mixture. Beginning 5 ...
... kindly supplied by Dr. Sasson Cohen and Dr. Aaron Bendich. All other haptens used were commercial products of standard purity. Immunoehemical l~rocedures.--Rabbits were injected once weekly for 3 weeks into the foot-pads (10) with Pur-BSA or Pur-HSA in complete Freund's adjuvant mixture. Beginning 5 ...
Promega Enzyme Resource Guide, Cloning Enzymes , BR075B
... having either complementary cohesive or blunt ends, and has an absolute requirement for ATP as a cofactor; it cannot use NAD. E. coli DNA ligase (which, like most prokaryotic DNA ligases uses NAD as a cofactor instead of ATP) can sometimes be used in place of T4 DNA ligase for ligation of single-str ...
... having either complementary cohesive or blunt ends, and has an absolute requirement for ATP as a cofactor; it cannot use NAD. E. coli DNA ligase (which, like most prokaryotic DNA ligases uses NAD as a cofactor instead of ATP) can sometimes be used in place of T4 DNA ligase for ligation of single-str ...
Programmed Cell Death during Leaf Senescence in Eucommia
... region, it is a very useful technique to detect in situ those nuclei in which the number of DNA ends has increased as a consequence of DNA degradation. In Figure2, the nuclei of mesophyll cells in green2 and yellow leaves were labeled by TUNEL (Fig.2, C and D) and green2 leaves were labeled stronger ...
... region, it is a very useful technique to detect in situ those nuclei in which the number of DNA ends has increased as a consequence of DNA degradation. In Figure2, the nuclei of mesophyll cells in green2 and yellow leaves were labeled by TUNEL (Fig.2, C and D) and green2 leaves were labeled stronger ...
Chapter 1
... ▪ Glycerol: a 3-carbon alcohol molecule ▪ Three clusters of carbon-chained atoms, termed fatty acids, attach to the glycerol molecule to form a ...
... ▪ Glycerol: a 3-carbon alcohol molecule ▪ Three clusters of carbon-chained atoms, termed fatty acids, attach to the glycerol molecule to form a ...
Chapter 3: DNA and the Genetic Code
... marks. For example, the nucleotide DNA triplets ATT, ATC, and ACT are analogous to a period (.) in ending a sentence—all three signal the end of a polypeptide chain. Other punctuation marks denote the start of the amino acid sequence for the peptide. Unlike the triplet nature of the DNA words for am ...
... marks. For example, the nucleotide DNA triplets ATT, ATC, and ACT are analogous to a period (.) in ending a sentence—all three signal the end of a polypeptide chain. Other punctuation marks denote the start of the amino acid sequence for the peptide. Unlike the triplet nature of the DNA words for am ...
Rapid and simple method for DNA extraction from plant and algal
... vitro and grown in a growth chamber; the DNA was subsequently tested by PCR amplification. We found that the transgene from the DNA of transgenic tomato plants was successfully amplified (Fig. 2a; lanes 12, 14, 16, 61, and 64) and that the samples without a band were from nontransgenic plants. This ...
... vitro and grown in a growth chamber; the DNA was subsequently tested by PCR amplification. We found that the transgene from the DNA of transgenic tomato plants was successfully amplified (Fig. 2a; lanes 12, 14, 16, 61, and 64) and that the samples without a band were from nontransgenic plants. This ...
Comparison of DNA extraction methods for Aspergillus fumigatus
... (Loffler et al., 1997). Loffler et al. (1997) compared a number of commercial kits and the Qiagen DNA Mini kit was reported to be the best available for the extraction and purification of DNA from fungi. Extra steps are still required initially to lyse the cell prior to purification, as fungal cell ...
... (Loffler et al., 1997). Loffler et al. (1997) compared a number of commercial kits and the Qiagen DNA Mini kit was reported to be the best available for the extraction and purification of DNA from fungi. Extra steps are still required initially to lyse the cell prior to purification, as fungal cell ...
eDNA GCN Analysis - SureScreen Scientifics
... we test it. Although the testing process takes a day, many laboratories wait until they have enough samples to make the cost economic, so results can sometimes take months. That’s a problem because you then have no option but to continue with surveys while you wait for the answer. We appreciate time ...
... we test it. Although the testing process takes a day, many laboratories wait until they have enough samples to make the cost economic, so results can sometimes take months. That’s a problem because you then have no option but to continue with surveys while you wait for the answer. We appreciate time ...
DNA replication - U of L Class Index
... The rate of fork movement in human cells, based on fiberlabeling experiments, is only about 100bp/second/fork. The entire human genome of 3 x 109 bp replicates in about 8 hours, suggesting that human genome might have about 1000 forks. However, fiber autoradiography and electron microscopy indicat ...
... The rate of fork movement in human cells, based on fiberlabeling experiments, is only about 100bp/second/fork. The entire human genome of 3 x 109 bp replicates in about 8 hours, suggesting that human genome might have about 1000 forks. However, fiber autoradiography and electron microscopy indicat ...
Genetic backgrounds of each Escherichia coli strain used
... mcrB+: McrB (modified cytosine restriction) system of E. coli interferes with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. Ref: http://www.ncbi.nlm.nih.gov/pubme ...
... mcrB+: McrB (modified cytosine restriction) system of E. coli interferes with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. Ref: http://www.ncbi.nlm.nih.gov/pubme ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""