Download Multiple choice questions

February 5, 2010
Multiple choice questions
(numbers in brackets indicate the number of correct answers)
Restriction fragment length polymorphisms (RFLPs)
Are used to determine the position of restriction sites in a genome
Are used in physical mapping
Are used in genetic mapping
Usually occur as multiple (more than 2) alleles in a genome
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(1)
Ribose is found in
Nucleic acids
Proteins
Lipids
RNA
DNA
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(2)
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Most RNA molecules in cells belong to
t-RNAs
mRNAs
ribosomal RNAs
repeat elements
small nuclear RNAs
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(1)
Hydrogen bonds
are covalent bonds
stabilize the DNA double helix
stabilize protein structures
form between two electronegative atoms
form between adenin and guanine
have a bond energy of 20-30 kcal mol-1
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(2)
The genetic code
is a triplet code
consists of 62 codons
has three stop codons
is used in the process of transcription
specifies 25 amino acids
is degenerate
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(3)
PCR is used for
reverse transcribing RNA into DNA
digesting proteins
digesting DNA
copying plasmids
amplifying DNA
amplifying proteins
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(1)
Microsatellites
are tandemly repeated sequences
are not very common in the human genome
can be used in genetic mapping of genomes
are usually longer than 200 bp
are normally found at the end of chromosomes
Transcriptomes
consist of RNA
consist of DNA
consist of proteins
are translated into proteins
do not change
differ in different cells
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-sheets are stabilized by
hydrophobic bonds
ionic bonds
hydrogen bonds
covalent bonds
all of the above
none of the above
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(3)
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The shotgun method
is used in analyzing transcriptomes
requires computers
is more accurate than the clone contig method
is normally used with large genomes
takes more time than other genome sequencing approaches
Restriction endonucleases
are located in the nucleus of cells
degrade DNA completely
bind to DNA
are enzymes
are proteins
were discovered in the 1980s
(2)
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Cloning vectors
are always plasmids
contain an origin of replication
have an average size of 20 kilobase pairs (kb)
are always circular molecules
contain always an antibiotics resistance gene
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(1)
(1)
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(3)
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(1)
Transformation
converts DNA into RNA
converts RNA into proteins
joins two DNA fragments
cuts DNA into fragments
introduces DNA into cells
removes genomes from cells
is used in cloning of DNA
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DNA polymerases
join DNA fragments
replicate RNA
replicate DNA
synthesize DNA in 5’->3’ direction
synthesize DNA in 3’->5’ direction
require a primer to function
require nucleotides to function
require ATP
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(2)
(4)
DNA
is a positively charged molecule at neutral pH
contains phosphate
is always double-stranded
has a diameter of around 2 µm
is stabilized by base stacking
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(2)
Simple sequence length polymorphisms
can be minisatellites
can be microsatellites
can be satellite DNA
are used in genetic mapping
are typed by oligonucleotide hybridization
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(3)
Bacteriophage lambda
is a virus
infects several species of bacteria
has a genome of about 20,000 bp
has three types of infection cycles
can only be seen in an electron microscope
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(2)
Total: (34)
In addition:
Multiple choice and short answer questions from chapters 1-3 of the Genomes 3
textbook.