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Transcript
REVIEW OF MOLECULAR GENETICS DR. EDELBERG Genes, DNA, & Chromosomes QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. Anatomy of DNA Gene anatomy-simple QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. Anatomy of a Gene QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. DNA LIBRARIES DNA library - a random collection of DNA fragments from an organism cloned into a vector Ideally contains at least one copy of every DNA sequence. Easily maintained in the laboratory Can be manipulated in various ways to facilitate the isolation of a DNA fragment of interest to a scientist. Numerous types of libraries exist for various organisms - Genomic and cDNA Genomic Library Construction of cDNA Library Genomic Vs. cDNA Library Genomic Library Promoters Introns Intergenic Non-expressed genes cDNA Library Expressed genes Transcription start sites Open reading frames (ORFs) Splice points Extraction of mRNA Collect and grind up animals in mild denaturing solution Spin out debris (Tissue, membranes, etc) Treat with DNAse (removes DNA) Treat with Phenol (removes protein) Synthesis of cDNA from mRNA Cloning DNA into vector Cloning into vectordetailed Cloned into multiple cloning site Plasmid Characteristics Ori Selectable Marker 3.6 Kb Color screen MCS Circular DNA Color Screen Transformation Over night
