Physiological Strategies During Animal Diapause: Lessons from
... Jason E. Podrabsky1, * and Steven C. Hand2, * ...
... Jason E. Podrabsky1, * and Steven C. Hand2, * ...
Health outcomes of children born after IVF/ICSI: a review of current
... The age-related diminution of the quality and fertility of oocytes is key in assisted reproduction: a high proportion of human oocytes have chromosome abnormalities and this increases with maternal age (Balasch and Gratacós, 2012; Cheung et al., 2011; Fragouli et al., 2011b; Johnson and Tough, 2012 ...
... The age-related diminution of the quality and fertility of oocytes is key in assisted reproduction: a high proportion of human oocytes have chromosome abnormalities and this increases with maternal age (Balasch and Gratacós, 2012; Cheung et al., 2011; Fragouli et al., 2011b; Johnson and Tough, 2012 ...
Neurotransmitter Transporters in the Central Nervous System
... as the enlargement of their fourth and sixth extra- ...
... as the enlargement of their fourth and sixth extra- ...
213 porters and neurotransmitter transporters
... Termination of neurotransmission Neurotransmission in mammals is primed by the synthesis of a specific neurotransmitter that is accumulated into synaptic vesicles or granules. Following a specific signal, the transmitter is secreted from the vesicles into the synaptic cleft (Kanner, 1989; Nelson, 19 ...
... Termination of neurotransmission Neurotransmission in mammals is primed by the synthesis of a specific neurotransmitter that is accumulated into synaptic vesicles or granules. Following a specific signal, the transmitter is secreted from the vesicles into the synaptic cleft (Kanner, 1989; Nelson, 19 ...
Tetraploid rescue - Development
... arm in common [i.e., monobrachial homology (MBH) for X chromosome], give rise to a high frequency of embryos with X chromosome aneuploidy, but do not give birth to either XXY or XXX pups (Tease and Fisher, 1993). During female mouse development, XM and XP chromosomes are often distinguished from eac ...
... arm in common [i.e., monobrachial homology (MBH) for X chromosome], give rise to a high frequency of embryos with X chromosome aneuploidy, but do not give birth to either XXY or XXX pups (Tease and Fisher, 1993). During female mouse development, XM and XP chromosomes are often distinguished from eac ...
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... medium and fixed immediately for electron microscopy. The fixation and embedding protocols of Stein & Stein (1971) for lipid high-resolution autoradiography were used in the present study. Thin sections were collected on copper grids and coated with Ilford L-4 nuclear emulsion. After a suitable expo ...
... medium and fixed immediately for electron microscopy. The fixation and embedding protocols of Stein & Stein (1971) for lipid high-resolution autoradiography were used in the present study. Thin sections were collected on copper grids and coated with Ilford L-4 nuclear emulsion. After a suitable expo ...
Good quality blastocyst from non-/mono
... there was a significant positive relationship between the cell numbers of embryos on D2 and D3 and blastocyst development [Neuber et al. 2003]. Therefore, we believe that the incidence of blastocysts with normal chromosomal composition is higher if the embryos are at 4-cell stage on D2 and/or at 6-8 ...
... there was a significant positive relationship between the cell numbers of embryos on D2 and D3 and blastocyst development [Neuber et al. 2003]. Therefore, we believe that the incidence of blastocysts with normal chromosomal composition is higher if the embryos are at 4-cell stage on D2 and/or at 6-8 ...
Automated sorting of live transgenic embryos
... studies, for example to determine whether a multiprotein complex forms or localizes correctly in vivo when one component is missing, and (3) the need for rapid genetic screening for gene expression changes in living embryos using a fluorescent protein reporter. Keywords: Drosophila, embryo, developm ...
... studies, for example to determine whether a multiprotein complex forms or localizes correctly in vivo when one component is missing, and (3) the need for rapid genetic screening for gene expression changes in living embryos using a fluorescent protein reporter. Keywords: Drosophila, embryo, developm ...
BLASTOCYST TRANSFER
... Blastocyst and derivation rate in relation to embryo origin and quality •Sjogren et al, RBM online 2004 •Findikli et al, RBM online 2005 •Mitalipova et al, Stem Cells 2004 (discarded embryos) •Chen et al, Hum Reprod 2005 •Kim et al, Stem Cells 2005 (derivation method depending on blastocyst quality ...
... Blastocyst and derivation rate in relation to embryo origin and quality •Sjogren et al, RBM online 2004 •Findikli et al, RBM online 2005 •Mitalipova et al, Stem Cells 2004 (discarded embryos) •Chen et al, Hum Reprod 2005 •Kim et al, Stem Cells 2005 (derivation method depending on blastocyst quality ...
Oxidative stress and protection against reactive oxygen
... NADPH oxidase: Production of superoxide anion and H2O2 via NADPH oxidase has been described on a rabbit blastocyst surface (Manes and Lai, 1995). Also, incubation of 2-cell mouse embryos with an inhibitor of NADPH oxidase induces a concentrationdependent reduction in H2O2 production (Nasr-Esfahani a ...
... NADPH oxidase: Production of superoxide anion and H2O2 via NADPH oxidase has been described on a rabbit blastocyst surface (Manes and Lai, 1995). Also, incubation of 2-cell mouse embryos with an inhibitor of NADPH oxidase induces a concentrationdependent reduction in H2O2 production (Nasr-Esfahani a ...
The Future of Human Embryo Culture Media – Or Have We
... 20 years of research before this problem was overcome. This research led to the discovery of the ‘substrate triad’ of pyruvate, lactate and glucose, as together they could support the development of some inbred mouse strain embryos from the 1-cell to the blastocyst (Bavister 1995). These substrates ...
... 20 years of research before this problem was overcome. This research led to the discovery of the ‘substrate triad’ of pyruvate, lactate and glucose, as together they could support the development of some inbred mouse strain embryos from the 1-cell to the blastocyst (Bavister 1995). These substrates ...
In vitro fertilization (IVF) in mammals: epigenetic and developmental
... Fertilization of eutherian animals occurs in the maternal oviduct. This is the natural and unique environment to achieve the necessary requirements for embryo life and its early and late development. The embryo conceived in vitro is manipulated and cultured in very different conditions [11, 16]. Con ...
... Fertilization of eutherian animals occurs in the maternal oviduct. This is the natural and unique environment to achieve the necessary requirements for embryo life and its early and late development. The embryo conceived in vitro is manipulated and cultured in very different conditions [11, 16]. Con ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
Metabolism in the pre-implantation oocyte and embryo
... vivo and usually with some degree of further in vitro manipulation. This includes physical removal from the follicle, exposure to culture media, sometimes combined with hyperstimulation to retrieve adequate numbers of COCs. This begs the question as to the influence of even brief exposure to in vitr ...
... vivo and usually with some degree of further in vitro manipulation. This includes physical removal from the follicle, exposure to culture media, sometimes combined with hyperstimulation to retrieve adequate numbers of COCs. This begs the question as to the influence of even brief exposure to in vitr ...
Analysis and nucleotide sequence of an origin of DNA replication in
... transformation efficiency.This indicates that no restriction barrier exists in A. calcoaceticus BD413 for E. coli-derived DNA. The yields of plasmid preparations from E. coli (up to 1/tg/ml) indicated that pWH 1266 has a high copy number, while it appears to be a low copy number plasmid in A. calcoa ...
... transformation efficiency.This indicates that no restriction barrier exists in A. calcoaceticus BD413 for E. coli-derived DNA. The yields of plasmid preparations from E. coli (up to 1/tg/ml) indicated that pWH 1266 has a high copy number, while it appears to be a low copy number plasmid in A. calcoa ...
The cell lineage of the muscles of the Drosophila head
... were observed (39 clones scored). Muscles not allotted to any set The following muscles could not be allotted to any of the three sets. Muscles 1, 2,14,15,16 and 24 (Fig. 1) were damaged during preparation of the samples and possible clones in them were therefore disregarded. Muscles 27 and 10 are v ...
... were observed (39 clones scored). Muscles not allotted to any set The following muscles could not be allotted to any of the three sets. Muscles 1, 2,14,15,16 and 24 (Fig. 1) were damaged during preparation of the samples and possible clones in them were therefore disregarded. Muscles 27 and 10 are v ...
Cloning and Expression of Cellulosimicrobium cellulans β
... Endo-1,3-β-glucanases (EC 3.2.1.6 and EC 3.2.1.39) are widely distributed among bacteria and higher plants [1]. These enzymes catalyse the hydrolysis of β-1,3-glucan component found in the yeast cell wall and other β-1,3glucans such as laminarin, curdlan and pachyman [2,3]. The bacterium Cellulosimi ...
... Endo-1,3-β-glucanases (EC 3.2.1.6 and EC 3.2.1.39) are widely distributed among bacteria and higher plants [1]. These enzymes catalyse the hydrolysis of β-1,3-glucan component found in the yeast cell wall and other β-1,3glucans such as laminarin, curdlan and pachyman [2,3]. The bacterium Cellulosimi ...
- Annals of Forest Science
... C. dupreziana embryos were diploid [35] and some of the enzymatic phenotypes observed in the present study corresponded to heterozygous embryos. A “paternal sexual reproduction” could also be hypothesized, where nuclei fusion occurs between male gametes produced by one or several pollen grains. Howe ...
... C. dupreziana embryos were diploid [35] and some of the enzymatic phenotypes observed in the present study corresponded to heterozygous embryos. A “paternal sexual reproduction” could also be hypothesized, where nuclei fusion occurs between male gametes produced by one or several pollen grains. Howe ...
Conference7 - Zodletone Spring
... • 11 fungal clones from the mat and crust clone libraries formed a novel fungal group together with several other clones encountered in a variety of anaerobic environments (from Lake Ketelmeer, Netherlands, and Berkeley Aquatic Park sediments, Berkeley, CA). ...
... • 11 fungal clones from the mat and crust clone libraries formed a novel fungal group together with several other clones encountered in a variety of anaerobic environments (from Lake Ketelmeer, Netherlands, and Berkeley Aquatic Park sediments, Berkeley, CA). ...
mRNA translation during oocyte maturation plays a key role in
... (PGCs) in Xenopus embryos. In this study, we sought to determine if translation of maternal mRNA during oocyte maturation is involved in the development of PGCs. Donor oocytes were collected from both stimulated (those who receive gonadotropin) and unstimulated females, artificially matured and fert ...
... (PGCs) in Xenopus embryos. In this study, we sought to determine if translation of maternal mRNA during oocyte maturation is involved in the development of PGCs. Donor oocytes were collected from both stimulated (those who receive gonadotropin) and unstimulated females, artificially matured and fert ...
Modern Genetics
... (Dolly was put down on Feb. 14, 2003. She suffered from lung cancer and crippling arthritis. She was 6 yrs old. Most Finn Dorset sheep live to be 11-12 yrs. old). ...
... (Dolly was put down on Feb. 14, 2003. She suffered from lung cancer and crippling arthritis. She was 6 yrs old. Most Finn Dorset sheep live to be 11-12 yrs. old). ...
Modern Genetics
... (Dolly was put down on Feb. 14, 2003. She suffered from lung cancer and crippling arthritis. She was 6 yrs old. Most Finn Dorset sheep live to be 11-12 yrs. old). ...
... (Dolly was put down on Feb. 14, 2003. She suffered from lung cancer and crippling arthritis. She was 6 yrs old. Most Finn Dorset sheep live to be 11-12 yrs. old). ...
MA112 Expresso® Rhamnose SUMO Cloning and
... their native sequences. Mutations in the SUMO tag render it resistant to cleavage by the native SUMO proteases found in eukaryotic cells (4, 5). Compensating mutations have been introduced into the SUMO Express Protease to restore full cleavage activity; only this engineered form of the protease can ...
... their native sequences. Mutations in the SUMO tag render it resistant to cleavage by the native SUMO proteases found in eukaryotic cells (4, 5). Compensating mutations have been introduced into the SUMO Express Protease to restore full cleavage activity; only this engineered form of the protease can ...
Cloning and characterization of the Xenopus laevis p8 gene
... (Fig. 2c) and, given that Xp8 is localized to the nucleus, it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-co ...
... (Fig. 2c) and, given that Xp8 is localized to the nucleus, it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-co ...
Definitions for annotating CDS sequences
... 3. Defining the Linker Sequences In the context of this analysis, “Linkers” refers to nucleotide sequences that flank the relevant CDS that will be evaluated on the nucleotide level but not at the amino acid level. From a molecular biology perspective, these are often thought of as “junction sequenc ...
... 3. Defining the Linker Sequences In the context of this analysis, “Linkers” refers to nucleotide sequences that flank the relevant CDS that will be evaluated on the nucleotide level but not at the amino acid level. From a molecular biology perspective, these are often thought of as “junction sequenc ...
Cloning
In biology, cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. The term also refers to the production of multiple copies of a product such as digital media or software.The term clone, invented by J. B. S. Haldane, is derived from the Ancient Greek word κλών klōn, ""twig"", referring to the process whereby a new plant can be created from a twig. In horticulture, the spelling clon was used until the twentieth century; the final e came into use to indicate the vowel is a ""long o"" instead of a ""short o"". Since the term entered the popular lexicon in a more general context, the spelling clone has been used exclusively.In botany, the term lusus was traditionally used.