Cloning a Gene for Over-expression and Purification
... ATG start codon present on the pET translation vectors.PflM A C-terminal His•Tag® sequence is availApaB isI(674) able. Unique sites are shown on the circle map. Note that the sequence numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expressi ...
... ATG start codon present on the pET translation vectors.PflM A C-terminal His•Tag® sequence is availApaB isI(674) able. Unique sites are shown on the circle map. Note that the sequence numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expressi ...
Protocol
... 2. Less cloning complexity. The single-strand DNA oligo encoding shRNA sequence is a perfect palindrome, and the same (two) palindromic oligos can anneal to each other to form a double-strand oligo. This eliminates the need to mix and anneal two different DNA oligos and reduces operational mistakes ...
... 2. Less cloning complexity. The single-strand DNA oligo encoding shRNA sequence is a perfect palindrome, and the same (two) palindromic oligos can anneal to each other to form a double-strand oligo. This eliminates the need to mix and anneal two different DNA oligos and reduces operational mistakes ...
Differential Gene Expression in the Gastrula of Xenopus Laevis
... possible nuclear precursor molecules (in kilobases) ...
... possible nuclear precursor molecules (in kilobases) ...
KOD -Plus
... blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201], KOD -Plus- Neo [Code No. KOD-401] or KOD FX [Code No. KFX-101]. The kit contains pTA2 Vector, 2x Ligation Buffer, T4 DNA Ligase and 10x A-attachment Mix. 10 x A-attachment mix is a reagent comprising anti-KOD DNA polymerase antibo ...
... blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201], KOD -Plus- Neo [Code No. KOD-401] or KOD FX [Code No. KFX-101]. The kit contains pTA2 Vector, 2x Ligation Buffer, T4 DNA Ligase and 10x A-attachment Mix. 10 x A-attachment mix is a reagent comprising anti-KOD DNA polymerase antibo ...
DNA Technology
... • Only a small fraction of blood cells are infected (1/10,000). • also require high degree of specificity while targeting conserved regions of DNA to guard against high level of genetic variability characteristic of retroviruses. • High risk of cross-contaminating sample with small amounts of amplif ...
... • Only a small fraction of blood cells are infected (1/10,000). • also require high degree of specificity while targeting conserved regions of DNA to guard against high level of genetic variability characteristic of retroviruses. • High risk of cross-contaminating sample with small amounts of amplif ...
Vectors for expression and modification of cDNA sequences in
... Vectors for expression and modification of cDNA sequences in Neurospora crassa. J.W. Campbell, C.S. Enderlin, and C.P. Selitrennikoff - Mycotox, Inc., 4200 E. Ninth Avenue, B111, Denver, CO 80262 The quinic acid inducible qa-2 promoter of Neurospora crassa has been used to express cloned genes by a ...
... Vectors for expression and modification of cDNA sequences in Neurospora crassa. J.W. Campbell, C.S. Enderlin, and C.P. Selitrennikoff - Mycotox, Inc., 4200 E. Ninth Avenue, B111, Denver, CO 80262 The quinic acid inducible qa-2 promoter of Neurospora crassa has been used to express cloned genes by a ...
Vectors: The carriers of DNA molecules DNA vectors and their
... 322), ligated in vitro to a plasmid or virus replicon from another species. Shuttle vectors can be made, for example, for E. coli/B. subtilis, E. coli/yeast or E. coli/mammalian cells. The shuttle vector strategy permits the exploitation of the many manipulative procedures, such as amplification, av ...
... 322), ligated in vitro to a plasmid or virus replicon from another species. Shuttle vectors can be made, for example, for E. coli/B. subtilis, E. coli/yeast or E. coli/mammalian cells. The shuttle vector strategy permits the exploitation of the many manipulative procedures, such as amplification, av ...
Introduction to Biotechnology
... • Immunoassay tests are used to test for the presence of contaminants in soil, water and even blood • Installation of biological barriers to prevent the transfer of harmful microorganisms between production ...
... • Immunoassay tests are used to test for the presence of contaminants in soil, water and even blood • Installation of biological barriers to prevent the transfer of harmful microorganisms between production ...
New Developments in the Embryology Laboratory
... nucleus (small nuclear RNAs)and in the nucleolus (small nucleolar RNAs) are important components of transcriptomics • Proportion of mRNAs in a transcriptome account for only 1-2% of total RNA content in somatic cells ...
... nucleus (small nuclear RNAs)and in the nucleolus (small nucleolar RNAs) are important components of transcriptomics • Proportion of mRNAs in a transcriptome account for only 1-2% of total RNA content in somatic cells ...
REPRODUCTION: THE CONTINUITY OF LIFE
... in the ovule to form a zygote. The other sperm nucleus combines with another bigger nucleus in the ovule which develops into the endosperm. Sexual Reproduction in Humans and Animals Humans (and all animals that reproduce sexually) have cells called gametes. Gametes are formed during meiosis and come ...
... in the ovule to form a zygote. The other sperm nucleus combines with another bigger nucleus in the ovule which develops into the endosperm. Sexual Reproduction in Humans and Animals Humans (and all animals that reproduce sexually) have cells called gametes. Gametes are formed during meiosis and come ...
Overview - BioMed Central
... e.g. ref 37). The M13 leader and 5’ UTR was created by annealing oligos G3HindNdeS and G3NcoA, extending with DNA polymerase, digesting and cloning into the Hind3 and NcoI sites of pSANG3. The region of hybridization is underlined in the sequence given below. G3HindNdeS is based on the sequence of M ...
... e.g. ref 37). The M13 leader and 5’ UTR was created by annealing oligos G3HindNdeS and G3NcoA, extending with DNA polymerase, digesting and cloning into the Hind3 and NcoI sites of pSANG3. The region of hybridization is underlined in the sequence given below. G3HindNdeS is based on the sequence of M ...
CRISPR-Cas9 Mouse Toolbox
... 3. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 4. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR 5. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR 6. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 7. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 8. AAV:ITR-U6-sgRNA ...
... 3. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 4. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR 5. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR 6. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 7. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 8. AAV:ITR-U6-sgRNA ...
Gene cloning of P43 surface protein of toxoplasma gondii tachyzoite
... intermediate hosts. The sexual and asexual cycle of parasite respectively takes place in the intestinal epithelial of the cat (as definitive host) and any warm blooded, like mammals and birds (as intermediate hosts) (Frankel et al, 1970). Previous studies have showed that the main human infection ca ...
... intermediate hosts. The sexual and asexual cycle of parasite respectively takes place in the intestinal epithelial of the cat (as definitive host) and any warm blooded, like mammals and birds (as intermediate hosts) (Frankel et al, 1970). Previous studies have showed that the main human infection ca ...
pSAT vectors: a modular series of plasmids for autofluorescent
... Assembly of several pSAT expression cassettes into single plasmids For expression of two autofluorescently-tagged proteins from a single plasmid, we first produced pSAT1-ECFP-C1-VIP1 by cloning the VIP1 ORF as a SalI-BamHI fragment from pSAT6EGFP-C1-VIP1 into pSAT1-ECFP-C1. The ECFP-VIP1 expression ...
... Assembly of several pSAT expression cassettes into single plasmids For expression of two autofluorescently-tagged proteins from a single plasmid, we first produced pSAT1-ECFP-C1-VIP1 by cloning the VIP1 ORF as a SalI-BamHI fragment from pSAT6EGFP-C1-VIP1 into pSAT1-ECFP-C1. The ECFP-VIP1 expression ...
Cloning and Expression of Endoglucanase genes from Trichoderma
... Na 2 EDTA, 100 mM NaCl, and 0.1% [w/v] SDS). The cells were centrifuged at 13,000 rpm for 5 minutes at 4°C. The supernatant was discarded and the cells were resuspended in 200 µl of breaking buffer. An equal volume of acid washed glass beads were added. The mixture was vortexed for 30 seconds, follo ...
... Na 2 EDTA, 100 mM NaCl, and 0.1% [w/v] SDS). The cells were centrifuged at 13,000 rpm for 5 minutes at 4°C. The supernatant was discarded and the cells were resuspended in 200 µl of breaking buffer. An equal volume of acid washed glass beads were added. The mixture was vortexed for 30 seconds, follo ...
Lesson 2 | Asexual Reproduction
... mammoth. Dr. Ian Barnes of the University of London stated that he now believes a mammoth will be cloned in his lifetime. Tikhonov, however, points out that whole cells are needed for cloning. He is doubtful that Lyuba’s cells, which endured freezing temperatures, are intact. Other scientists note t ...
... mammoth. Dr. Ian Barnes of the University of London stated that he now believes a mammoth will be cloned in his lifetime. Tikhonov, however, points out that whole cells are needed for cloning. He is doubtful that Lyuba’s cells, which endured freezing temperatures, are intact. Other scientists note t ...
Prediction of Blastulation
... commonly used to assess embryo viability on day 3 in assisted reproduction clinics may both underestimate and overestimate embryo potential, with well-documented consequences, such as multiple births, the need for fetal reduction and miscarriage” Racowsky, C. High rates of embryonic loss, yet high i ...
... commonly used to assess embryo viability on day 3 in assisted reproduction clinics may both underestimate and overestimate embryo potential, with well-documented consequences, such as multiple births, the need for fetal reduction and miscarriage” Racowsky, C. High rates of embryonic loss, yet high i ...
Reproduction in Plants and animals
... Definition of reproduction: Reproduction is the biological process by which new offspring/ individual organisms are produced from their parents. Reproduction is a characteristic of all living organisms; each individual organism exists as the result of reproduction. Reproduction is necessary for the ...
... Definition of reproduction: Reproduction is the biological process by which new offspring/ individual organisms are produced from their parents. Reproduction is a characteristic of all living organisms; each individual organism exists as the result of reproduction. Reproduction is necessary for the ...
Reproduction
... What are the types of asexual reproduction? Budding: offspring grow out of the body of the parent (unequal division). Examples: hydra, tapeworm, and ...
... What are the types of asexual reproduction? Budding: offspring grow out of the body of the parent (unequal division). Examples: hydra, tapeworm, and ...
Construction of an arabidopsis BAC library and isolation of clones
... disease-resistance genes. This clone (ATTS0477) displays significant similarity to the disease-resistance genes RPM1 and RPS2 (Grant et al., 1995; Bent et al., 1995), and co-segregates with the disease-resistance gene RPS5 (Siminoch and Innes, 1995; E. Drenkard and F. Ausubel, personal communication ...
... disease-resistance genes. This clone (ATTS0477) displays significant similarity to the disease-resistance genes RPM1 and RPS2 (Grant et al., 1995; Bent et al., 1995), and co-segregates with the disease-resistance gene RPS5 (Siminoch and Innes, 1995; E. Drenkard and F. Ausubel, personal communication ...
Asexual Reproduction
... medium such as water, which the sperms can use to swim towards the egg cell. External fertilization usually occur in fish and amphibians. The females lay the eggs in the water and the male squirts the sperm in the same area. ...
... medium such as water, which the sperms can use to swim towards the egg cell. External fertilization usually occur in fish and amphibians. The females lay the eggs in the water and the male squirts the sperm in the same area. ...
Cloning
In biology, cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. The term also refers to the production of multiple copies of a product such as digital media or software.The term clone, invented by J. B. S. Haldane, is derived from the Ancient Greek word κλών klōn, ""twig"", referring to the process whereby a new plant can be created from a twig. In horticulture, the spelling clon was used until the twentieth century; the final e came into use to indicate the vowel is a ""long o"" instead of a ""short o"". Since the term entered the popular lexicon in a more general context, the spelling clone has been used exclusively.In botany, the term lusus was traditionally used.