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Transcript
Review for Exam 4 Jones and Bartlett Publishers © 2005 Terms to know: Ch. 10 aminoacyl-tRNA synthetase polycistronic DNA anticodon polysome E/P/A sites of ribosome primary transcript charged tRNA promoter coding sequence RNA processing exon/intron snRNP exon-shuffle model translation lariat structure wobble polyA tail Terms to know: Ch. 11 anti-sense RNA enhancer aporepressor epigenetic attenuation housekeeping genes beta-galactosidase inducer cAMP-CRP complex operator chromatin-remodeling complex operon constitutive mutant repressor CpG island silencer DNA methylation antibody parts Terms to know: Ch. 12 BAC genomics bioinformatics library Blunt/sticky end proteomics cloned DNA reporter gene cDNA STS contig Ti plasmid DNA microarray transgenic organism EST vector knockout mutation polylinker Discussion topics • Know the details of transcription and translation – enzymes involved, what is produced, where the process happens, etc. (about 50% of the exam). • Know why the genetic ‘code’ is said to be degenerate (or redundant), and what wobble is. • Know the different ways genetic expression is regulated. • Know the lac operon details. • In addition to the terms in Chapter 12, know how reverse transcriptase is used to make cDNA, and how this is helpful. • Also, know the ten fill-in-the-blank questions for each of the three chapters.
